Article
Biochemistry & Molecular Biology
Ivan Ponce, Nelson Garrido, Nicolas Tobar, Francisco Melo, Patricio C. Smith, Jorge Martinez
Summary: The research revealed that increased tissue stiffness stimulates lactate production and glucose uptake by mammary fibroblasts, which is correlated with the expression of specific proteins. Additionally, mammary stromal cells cultured on stiff matrices generated factors that stimulated epithelial breast migration.
Article
Multidisciplinary Sciences
Gang Cheng, Micael Hardy, Balaraman Kalyanaraman
Summary: N-acetylcysteine (NAC) is an antioxidant drug that has been used in tumor cells and preclinical mice tumor xenografts. A new thiol-containing molecule, Mito(10)-NAC, has been synthesized and found to be nearly 2000-fold more effective than NAC in inhibiting cancer cell proliferation, including pancreatic cancer cells.
SCIENTIFIC REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Holger M. Becker, Joachim W. Deitmer
Summary: Intra- and extracellular pH regulation is crucial for cells and tissues, with distorted pH regulation in tumor tissues creating a hostile environment for cancer cells. Cancer cells manipulate a variety of transporters and carbonic anhydrases to alter pH values, promoting tumor progression. Transport metabolons formed by these components play a key role in tumor pH regulation and potential therapeutic interventions for cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marta Baczewska, Elzbieta Supruniuk, Klaudia Bojczuk, Pawel Guzik, Patrycja Milewska, Katarzyna Kononczuk, Jakub Dobroch, Adrian Chabowski, Pawel Knapp
Summary: The study aimed to assess the dominant energy substrate transport mechanism in ovarian cancer cells and predict clinical outcomes using genomic aberrations. The researchers found differential gene expression in high-grade serous carcinoma and suggested the potential use of glucose and lactate transport as therapeutic targets to impede ovarian cancer growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yi Rao, Seth T. Gammon, Margie N. Sutton, Niki M. Zacharias, Pratip Bhattacharya, David Piwnica-Worms
Summary: This study reveals that high concentrations of exogenous pyruvate inhibit intracellular LDH activity and glycolytic ECAR, with the extent of inhibition being highly dependent on pyruvate influx mediated by monocarboxylate transporter-1. These findings suggest that maintaining plasma pyruvate at submillimolar concentrations could minimize transient metabolic perturbations and enhance the accuracy of metabolic studies using hyperpolarized [1-C-13]pyruvate magnetic resonance spectroscopic imaging and stable isotope tracer experiments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Vicente Medel, Nicolas Crossley, Ivana Gajardo, Eli Muller, L. Felipe Barros, James M. Shine, Jimena Sierralta
Summary: By analyzing gene expression data, we found that neuronal MCT2 lactate transporter is enriched in the cerebral cortex and negatively correlated with cortical thickness. MCT2 expression is associated with glucose utilization and brain function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ehsan Sohrabi, Masoumeh Moslemi, Ehsan Rezaie, Nahid Nafissi, Mansoor Khaledi, Hamed Afkhami, Javad Fathi, Ali Zekri
Summary: The study evaluated the expression of MCT3 (SLC16A8), MCT8 (SLC16A2), and MCT9 (SLC16A9) genes in breast cancer samples and compared to normal adjacent tissues. The results showed significantly lower expression of the MCT3 gene in breast cancer tissue, suggesting potential as a therapeutic target in breast cancer patients.
Article
Oncology
Vera Miranda-Goncalves, Celine S. Goncalves, Sara Granja, Joana Vieira de Castro, Rui M. Reis, Bruno M. Costa, Fatima Baltazar
Summary: The overexpression of MCT1 is associated with poor prognosis in GBM patients. Inhibiting MCT1 can slow down tumor growth and enhance response to temozolomide treatment in GBM.
Article
Multidisciplinary Sciences
Yu Ri Seo, Junghyeon Lee, Han Suk Ryu, EunHee G. Kim, So Hyun Kim, Jieun Jeong, Hyeryeon Jung, Yeojin Jung, Han Byeol Kim, Yeon Hui Jo, Yeong Dong Kim, Min-Sun Jin, Yong Yook Lee, Kristine M. Kim, Eugene C. Yi
Summary: This study analyzed the cell-surface membrane proteins interacting with CD147 in cancer stem cells using a proximity-labeling proteomic approach. The lateral interaction between CD147 and CD276 was found to confer resistance to docetaxel in breast cancer. Co-expression of CD147 and CD276 was associated with poor survival rates and a poor response to chemotherapy in breast cancer patients.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Xiao Hu, Zhanzhao Liu, Xianxian Duan, Xiao Han, Mengci Yuan, Lingyan Liu, Xiaojun Xia, Ning Li, Junfang Qin, Yue Wang
Summary: The study revealed that SUMOylation of MCT4 stabilizes its protein levels in breast cancer, with K448 being crucial for this process. Mutations of K448 in MCT4 could potentially have therapeutic significance in breast cancer treatment.
MOLECULAR CARCINOGENESIS
(2021)
Article
Oncology
Xiangru Li, Xiaoju Zhou, Ying Liu, Jingjing Fan, Hongjing Huo, Jingjing Yao, Lin Wang, Ningning Ma
Summary: Metastasis is a major contributing factor to low cancer patient survival rates, and overexpression of MCT4 promotes cancer cell migration and invasion by modulating the EGF/EGFR pathway.
Article
Biochemistry & Molecular Biology
Julianne D. Twomey, Baolin Zhang
Summary: Circulating tumor cells (CTCs) are the source of metastasis and can be used as a liquid biopsy for monitoring cancer progression and treatment response. However, accurately detecting CTCs has been challenging due to their low frequency and heterogeneity. In this study, an in vitro model of CTCs was developed using non-adherent suspension culture to study breast cancer cell lines of different molecular subtypes. It was found that detached breast cancer cells accumulated cancer stem cells (CSCs) expressing stem cell markers and exhibiting high expressions of hypoxia-inducible gene products, especially CAIX. Inhibiting CAIX activity reduced CAIX expression and stem cell phenotypes in the targeted CTCs.
Article
Oncology
Arne Van der Vreken, Inge Oudaert, Gamze Ates, Sylvia Faict, Philip Vlummens, Hatice Satilmis, Rong Fan, Anke Maes, Ann Massie, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Eline Menu
Summary: This study suggests an alternative combination treatment for multiple myeloma by targeting metabolic pathways. The combination of syrosingopine and metformin shows strong cytotoxic effects on multiple myeloma cell lines and patient samples, suppressing both glycolysis and oxidative phosphorylation. Furthermore, the combination treatment leads to a significant reduction in tumor burden in vivo.
JOURNAL OF PATHOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Michiko Yamashita, Udo R. Markert
Summary: This review summarizes the current knowledge of drug transporters in the human placenta, emphasizing the importance for the health of pregnant women and their fetuses in need of drug treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Balaraman Kalyanaraman, Gang Cheng, Micael Hardy
Summary: This review focuses on the vulnerabilities of tumor cells and immune cells in the tumor immune microenvironment and discusses the therapeutic opportunities associated with these vulnerabilities.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ashok Aspatwar, Holger M. Becker, Nanda Kumar Parvathaneni, Milka Hammaren, Aleksandra Svorjova, Harlan Barker, Claudiu T. Supuran, Ludwig Dubois, Philippe Lambin, Mataleena Parikka, Seppo Parkkila, Jean-Yves Winum
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Cyril Corbet, Estelle Bastien, Nihed Draoui, Bastien Doix, Lionel Mignion, Benedicte F. Jordan, Arnaud Marchand, Jean-Christophe Vanherck, Patrick Chaltin, Olivier Schakman, Holger M. Becker, Olivier Riant, Olivier Feron
NATURE COMMUNICATIONS
(2018)
Article
Biology
Sina Ibne Noor, Somayeh Jamali, Samantha Ames, Silke Langer, Joachim W. Deitmer, Holger M. Becker
Article
Biochemistry & Molecular Biology
Linda S. Forero-Quintero, Samantha Ames, Hans-Peter Schneider, Anne Thyssen, Christopher D. Boone, Jacob T. Andring, Robert McKenna, Joseph R. Casey, Joachim W. Deitmer, Holger M. Becker
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Ashok Aspatwar, Martti E. E. Tolvanen, Hans-Peter Schneider, Holger M. Becker, Susanna Narkilahti, Seppo Parkkila, Joachim W. Deitmer
Article
Cell Biology
Kathrin Renner, Christina Bruss, Annette Schnell, Gudrun Koehl, Holger M. Becker, Matthias Fante, Ayse-Nur Menevse, Nathalie Kauer, Raquel Blazquez, Lisa Hacker, Sonja-Maria Decking, Toszka Bohn, Stephanie Faerber, Katja Evert, Lisa Aigle, Sabine Amslinger, Maria Landa, Oscar Krijgsman, Elisa A. Rozeman, Christina Brummer, Peter J. Siska, Katrin Singer, Stefanie Pektor, Matthias Miederer, Katrin Peter, Eva Gottfried, Wolfgang Herr, Ibtisam Marchiq, Jacques Pouyssegur, William R. Roush, SuFey Ong, Sarah Warren, Tobias Pukrop, Philipp Beckhove, Sven A. Lang, Tobias Bopp, Christian U. Blank, John L. Cleveland, Peter J. Oefner, Katja Dettmer, Mark Selby, Marina Kreutz
Review
Oncology
Holger M. Becker
BRITISH JOURNAL OF CANCER
(2020)
Review
Neurosciences
Joachim W. Deitmer, Shefeeq M. Thepraambil, Ivan Ruminot, Sina Noor, Holger M. Becker
FRONTIERS IN NEUROSCIENCE
(2019)
Review
Oncology
Holger M. Becker, Joachim W. Deitmer
Article
Behavioral Sciences
Ellen McMullen, Astrid Weiler, Holger M. Becker, Stefanie Schirmeier
Summary: Neuronal function requires efficient transport of nutrients into the CNS with protection from unwanted solutes. The Drosophila BBB is formed by specific glial cells that separate the brain from the hemolymph, facilitating the uptake of glucose and trehalose. Loss of BBB transporters MFS3 and Pippin results in pupal lethality, but compensation through upregulation of Tret1-1 rescues transport efficiency and lethality, showing differences between RNA interference and null mutation compensatory mechanisms.
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Holger M. Becker, Joachim W. Deitmer
Summary: Intra- and extracellular pH regulation is crucial for cells and tissues, with distorted pH regulation in tumor tissues creating a hostile environment for cancer cells. Cancer cells manipulate a variety of transporters and carbonic anhydrases to alter pH values, promoting tumor progression. Transport metabolons formed by these components play a key role in tumor pH regulation and potential therapeutic interventions for cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Justina Kazokaite-Adomaitiene, Holger M. Becker, Joana Smirnoviene, Ludwig J. Dubois, Daumantas Matulis
Summary: This review presents the design, development, and identification of selective nano-to picomolar CA IX inhibitors VD11-4-2, VR16-09, and VD12-09. Various methods and results were discussed, including compound binding, biophysical techniques, protein cloning and purification strategies, and cancer cell-based assays which confirmed nanomolar activities of lead inhibitors. Novel CA IX inhibitors show promise for in vivo exploration and targeting multiple proteins involved in tumor acidosis may improve cancer management.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jurgita Matuliene, Gediminas Zvinys, Vytautas Petrauskas, Agne Kvietkauskaite, Audrius Zaksauskas, Kirill Shubin, Asta Zubriene, Lina Baranauskiene, Lina Kacenauskaite, Sergei Kopanchuk, Santa Veiksina, Vaida Paketuryte-Latve, Joana Smirnoviene, Vaida Juozapaitiene, Aurelija Mickeviciute, Vilma Michailoviene, Jelena Jachno, Dovile Stravinskiene, Aiste Sliziene, Agne Petrosiute, Holger M. Becker, Justina Kazokaite-Adomaitiene, Ala Yaromina, Edita Capkauskaite, Ago Rinken, Virginija Dudutiene, Ludwig J. Dubois, Daumantas Matulis
Summary: This study designed a series of high affinity and high selectivity fluorescein-labeled compounds targeting CAIX for visualization and quantification of CAIX expression in cancer cells. The competitive binding model was used to determine the dissociation constants of common CA inhibitors for CAIX in cancer cells.
SCIENTIFIC REPORTS
(2022)
Article
Mathematical & Computational Biology
Sandesh Athni Hiremath, Christina Surulescu, Somayeh Jamali, Samantha Ames, Joachim W. Deitmer, Holger M. Becker
MATHEMATICAL BIOSCIENCES AND ENGINEERING
(2019)