One-step site-specific antibody fragment auto-conjugation using SNAP-tag technology

Impact of Site-Specific Conjugation of ScFv to Multifunctional Nanomedicines Using Second Generation Maleimide

(2018) Katel Hervé-Aubert et al.   BIOCONJUGATE CHEMISTRY

Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons

(2018) Rahul Dhandapani et al.   Nature Communications

Recent progress in enzymatic protein labelling techniques and their applications

(2018) Yi Zhang et al.   CHEMICAL SOCIETY REVIEWS

Simultaneous and Independent Dual Site-Specific Self-Labeling of Recombinant Antibodies

(2018) Carolin Wollschlaeger et al.   BIOCONJUGATE CHEMISTRY

Beyond O6-Benzylguanine: O6-(5-Pyridylmethyl)guanine as a Substrate for the Self-Labeling Enzyme SNAP-Tag

(2018) Miguel Macias-Contreras et al.   BIOCONJUGATE CHEMISTRY

Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer

(2017) Claudia Kessler et al.   JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY

Spirohexene-Tetrazine Ligation Enables Bioorthogonal Labeling of Class B G Protein-Coupled Receptors in Live Cells

(2017) Carlo P. Ramil et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Development and applications of a near-infrared dye–benzylguanine conjugate to specifically label SNAP-tagged proteins

(2017) Xinbo Song et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Detection and Specific Elimination of EGFR+ Ovarian Cancer Cells Using a Near Infrared Photoimmunotheranostic Approach

(2017) Dirk Bauerschlag et al.   PHARMACEUTICAL RESEARCH

Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates

(2017) Alex R. Nanna et al.   Nature Communications

Monitoring Nitric Oxide in Subcellular Compartments by Hybrid Probe Based on Rhodamine Spirolactam and SNAP-tag

(2016) Chao Wang et al.   ACS Chemical Biology

SNAP-Tag Technology: A Useful Tool To Determine Affinity Constants and Other Functional Parameters of Novel Antibody Fragments

(2016) Judith Niesen et al.   BIOCONJUGATE CHEMISTRY

The efficient elimination of solid tumor cells by EGFR-specific and HER2-specific scFv-SNAP fusion proteins conjugated to benzylguanine-modified auristatin F

(2016) Mira Woitok et al.   CANCER LETTERS

Engineering antibody therapeutics

(2016) Mark L Chiu et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Peptide-tags for site-specific protein labelling in vitro and in vivo

(2016) Jonathan Lotze et al.   Molecular BioSystems

Water-soluble allyl sulfones for dual site-specific labelling of proteins and cyclic peptides

(2016) Tao Wang et al.   Chemical Science

Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand

(2016) Manal Amoury et al.   Oncotarget

A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis

(2016) Sylwia A. Mankowska et al.   Scientific Reports

Antibody-drug conjugates: recent advances in conjugation and linker chemistries

(2016) Kyoji Tsuchikama et al.   Protein & Cell

Site-Specific Dual Antibody Conjugation via Engineered Cysteine and Selenocysteine Residues

(2015) Xiuling Li et al.   BIOCONJUGATE CHEMISTRY

HaloTag Technology: A Versatile Platform for Biomedical Applications

(2015) Christopher G. England et al.   BIOCONJUGATE CHEMISTRY

Current methods for the synthesis of homogeneous antibody–drug conjugates

(2015) Alicja M. Sochaj et al.   BIOTECHNOLOGY ADVANCES

Novel EGFR-specific immunotoxins based on panitumumab and cetuximab show in vitro and ex vivo activity against different tumor entities

(2015) Judith Niesen et al.   JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY

Antibody Conjugates with Unnatural Amino Acids

(2015) Trevor J. Hallam et al.   MOLECULAR PHARMACEUTICS

Principles in the design of ligand-targeted cancer therapeutics and imaging agents

(2015) Madduri Srinivasarao et al.   NATURE REVIEWS DRUG DISCOVERY

A Fluorogenic Probe for SNAP-Tagged Plasma Membrane Proteins Based on the Solvatochromic Molecule Nile Red

(2014) Efthymia Prifti et al.   ACS Chemical Biology

Negative Electrospray Ionization via Deprotonation: Predicting the Ionization Efficiency

(2014) Anneli Kruve et al.   ANALYTICAL CHEMISTRY

Site-Specific Labeling of Cysteine-Tagged Camelid Single-Domain Antibody-Fragments for Use in Molecular Imaging

(2014) Sam Massa et al.   BIOCONJUGATE CHEMISTRY

Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes

(2014) Penelope M. Drake et al.   BIOCONJUGATE CHEMISTRY

Evaluation of Fluorophores to Label SNAP-Tag Fused Proteins for Multicolor Single-Molecule Tracking Microscopy in Live Cells

(2014) Peter J. Bosch et al.   BIOPHYSICAL JOURNAL

Metabolic Engineering of Monoclonal Antibody Carbohydrates for Antibody–Drug Conjugation

(2013) Nicole M. Okeley et al.   BIOCONJUGATE CHEMISTRY

Targeted Delivery of Dendritic Polyglycerol–Doxorubicin Conjugates by scFv-SNAP Fusion Protein Suppresses EGFR+ Cancer Cell Growth

(2013) Ahmad Fawzi Hussain et al.   BIOMACROMOLECULES

SNAP-tag based Agents for Preclinical In Vitro Imaging in Malignant Diseases

(2013) Manal Amoury et al.   CURRENT PHARMACEUTICAL DESIGN

Site-specific antibody drug conjugates for cancer therapy

(2013) Siler Panowski et al.   mAbs

Development of a split SNAP-tag protein complementation assay for visualization of protein–protein interactions in living cells

(2012) Masayasu Mie et al.   ANALYST

Sensitive Detection of Intracellular Sumoylation via SNAP Tag-Mediated Translation and RNA Polymerase-Based Amplification

(2012) Yong Yang et al.   ANALYTICAL CHEMISTRY

Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG

(2012) Amy B. Foraker et al.   JOURNAL OF CELL BIOLOGY

Synthesis of Bispecific Antibodies using Genetically Encoded Unnatural Amino Acids

(2012) Chan Hyuk Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-specific chemical protein conjugation using genetically encoded aldehyde tags

(2012) David Rabuka et al.   Nature Protocols

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SNAP-Tag Technology Mediates Site Specific Conjugation of Antibody Fragments with a Photosensitizer and Improves Target Specific Phototoxicity in Tumor Cells

(2011) Ahmad Fawzi Hussain et al.   BIOCONJUGATE CHEMISTRY

Engineering of an anti-epidermal growth factor receptor antibody to single chain format and labeling by sortase A-mediated protein ligation

(2011) Mariusz P. Madej et al.   BIOTECHNOLOGY AND BIOENGINEERING

Measuring In Vivo Protein Half-Life

(2011) Karolina Bojkowska et al.   CHEMISTRY & BIOLOGY

Cancer cell–selective in vivo near infrared photoimmunotherapy targeting specific membrane molecules

(2011) Makoto Mitsunaga et al.   NATURE MEDICINE

Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging

(2011) Graham T Dempsey et al.   NATURE METHODS

Rapid optical imaging of EGF receptor expression with a single-chain antibody SNAP-tag fusion protein

(2010) Florian Kampmeier et al.   EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING

Live-cell dSTORM with SNAP-tag fusion proteins

(2010) Teresa Klein et al.   NATURE METHODS

Site-Specific, Covalent Labeling of Recombinant Antibody Fragments via Fusion to an Engineered Version of 6-O-Alkylguanine DNA Alkyltransferase

(2009) Florian Kampmeier et al.   BIOCONJUGATE CHEMISTRY

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY