期刊
CHEMISTRY & BIOLOGY
卷 18, 期 6, 页码 805-815出版社
CELL PRESS
DOI: 10.1016/j.chembiol.2011.03.014
关键词
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资金
- Swiss National Science Foundation
- Federal Office for Professional Education and Technology (CTI) [8440]
- Fondation Claude et Giuliana
Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident intracellular and extracellular proteins in living mice. First, we demonstrate that SNAP-tag substrates have wide bioavailability in mice and can be used for the specific in vivo labeling of SNAP-tag fusion proteins. We then apply near-infrared probes to perform noninvasive imaging of in vivo-labeled tumors. Finally, we use SNAP-mediated chemical pulse-chase labeling to perform measurement of the in vivo half-life of different extra- and intracellular proteins. These results open broad perspectives for studying protein function in living animals.
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