期刊
NATURE IMMUNOLOGY
卷 20, 期 11, 页码 1530-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41590-019-0489-8
关键词
-
类别
资金
- CNRS
- INSERM
- European Research Council (ERC) [322465]
- European Union [787300]
- Agence Nationale de la Recherche
- MSDAVENIR Fund
- Investissement d'Avenir program of the French Ministry of Research ProFI [ANR-10-INBS-08]
- PHENOMIN (French National Infrastructure) [ANR-10-INBS-07]
- National Natural Science Foundation of China [81471595, 31400759]
- Education Department of Henan Province, China [16HASTIT030]
- INTEGRATE
- MSDAVENIR
- PHENOMIN
- European Research Council (ERC) [787300] Funding Source: European Research Council (ERC)
The activation of T cells by the T cell antigen receptor (TCR) results in the formation of signaling protein complexes (signalosomes), the composition of which has not been analyzed at a systems level. Here, we isolated primary CD4(+) T cells from 15 gene-targeted mice, each expressing one tagged form of a canonical protein of the TCR-signaling pathway. Using affinity purification coupled with mass spectrometry, we analyzed the composition and dynamics of the signalosomes assembling around each of the tagged proteins over 600 s of TCR engagement. We showed that the TCR signal-transduction network comprises at least 277 unique proteins involved in 366 high-confidence interactions, and that TCR signals diversify extensively at the level of the plasma membrane. Integrating the cellular abundance of the interacting proteins and their interaction stoichiometry provided a quantitative and contextual view of each documented interaction, permitting anticipation of whether ablation of a single interacting protein can impinge on the whole TCR signal-transduction network.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据