Article
Biochemistry & Molecular Biology
Diego Carballares, Javier Rocha-Martin, Roberto Fernandez-Lafuente
Summary: By chemically aminating immobilized TLL, Lecitase can be ionically exchanged and released from the biocatalyst, allowing the utilization of the fully active TLL to build a new combibiocatalyst. However, the release of Lecitase is not possible using high ionic strength solutions alone, requiring the use of octyl-vinylsulfone supports for irreversible immobilization of TLL.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Shiyan Wang, Lingxuan Duan, Liang Jiang, Kailong Liu, Shizhen Wang
Summary: A new strategy for assembling amino acid dehydrogenase with peptide linker onto metal-organic framework (MOF) ZIF-8 has been successfully developed, leading to improved enzyme activity and stability. The catalytic efficiency of PheDH_1D02/ZIF-8 is significantly higher than other controls, demonstrating that this method is simple, cost-effective, and efficient for enhancing enzyme stability.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Vivek C. Badgujar, Kirtikumar C. Badgujar, Pravin M. Yeole, Bhalchandra M. Bhanage
Summary: The study focuses on the synthesis of lab-made carboxymethyl cellulose (CMC) and chitosan (CHI) based co-polymer cross-linked with glutaraldehyde (GLU) for immobilization of Candida rugosa (CRL) lipase (CMC:CHI:GLU:CRL). The immobilized biocatalyst showed significant activity-stability and half-life-time in ultrasonicated medium, enabling excellent conversion of biomass-derived furfuryl derivative. Further optimization of synthetic protocol and recyclability study demonstrated the efficiency of the developed biocatalyst in green and sustainable synthesis.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Oliwia Degorska, Daria Szada, Agata Zdarta, Wojciech Smulek, Teofil Jesionowski, Jakub Zdarta
Summary: In this study, lipase immobilized from Aspergillus niger by physical immobilization and mixed physical immobilization and ion exchange was used for kinetic resolution of the ketoprofen racemic mixture. The immobilized lipase showed improved activity and stability, and phosphate buffer at pH 7 was found to be the best solvent.
Review
Environmental Sciences
Xing Zhu, Chenxi Du, Bo Gao, Bin He
Summary: High carbon dioxide concentration in the atmosphere calls for eco-friendly mitigation strategies. Carbonic anhydrase (CA) immobilization can enhance its stability and enzyme recycling, but there is limited mass transfer between gaseous CO2 and the active center of immobilized CA. This review focuses on improving the mass transfer process to increase the efficiency of enzymatic CO2 capture for industrial applications.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Chemistry, Multidisciplinary
Sebastian C. Cosgrove, Gavin J. Miller, Amin Bornadel, Beatriz Dominguez
Summary: The immobilization of nitroreductase enzyme enables continuous chemoenzymatic synthesis of anilines. The application of biocatalysis in classical synthetic transformations has gained attention due to the sustainability benefits it offers to the chemical industry. However, the biocatalytic reduction of aromatic nitro compounds using nitroreductase biocatalysts has not been extensively explored in synthetic chemistry. This study demonstrates the successful reduction of aromatic nitro compounds using immobilized nitroreductase in a continuous packed-bed reactor, allowing for extended reuse of the immobilized system.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Johannes Gottschalk, Miriam Assmann, Juergen Kuballa, Lothar Elling
Summary: The study focused on enzymatic synthesis of industrial hyaluronic acid, using immobilized cascade enzymes on magnetic beads to increase productivity and facilitate enzyme recovery. Results showed successful production of high-quality HA in repetitive batches.
Article
Chemistry, Physical
Christopher K. Prier, Karla Camacho Soto, Jacob H. Forstater, Nadine Kuhl, Jeffrey T. Kuethe, Wai Ling Cheung-Lee, Michael J. Di Maso, Claire M. Eberle, Shane T. Grosser, Hsing- Ho, Erik Hoyt, Anne Maguire, Kevin M. Maloney, Amanda Makarewicz, Jonathan P. McMullen, Jeffrey C. Moore, Grant S. Murphy, Karthik Narsimhan, Weilan Pan, Nelo R. Rivera, Anumita Saha-Shah, David A. Thaisrivongs, Deeptak Verma, Adeya Wyatt, Daniel Zewge
Summary: Enzymes immobilized on solid supports in organic solvents have been used to catalyze a biocatalytic transaminase reaction, resulting in efficient and selective chemical synthesis. The advantages of enzyme immobilization include improved reaction efficiency, enhanced enzyme stability, reaction in non-aqueous media, and simplified product separation. By combining directed enzyme evolution with optimal reaction solvent and solid support for immobilization, the transaminase reaction of Cyrene (2) was successfully carried out, producing the amine intermediate 3a en route to the BTK inhibitor nemtabrutinib as a crystalline salt with dr > 50:1.
Article
Chemistry, Physical
Daniel A. Grajales-Hernandez, Eleftheria Diamanti, Riccardo Moro, Susana Velasco-Lozano, Elisabet Pires, Fernando Lopez-Gallego
Summary: In this study, a multienzymatic system was designed to synthesize drugs, and the reaction efficiency and product purity were improved by controlling the spatial organization of enzymes. The results showed that optimal assembly of biocatalysts can alleviate inhibition and enhance reaction rate. Furthermore, integrating a coenzyme regeneration system with the biocatalyst allowed large-scale synthesis of the substrate.
Article
Chemistry, Multidisciplinary
Satyadip Paul, Mani Gupta, Kaushik Dey, Ashok Kumar Mahato, Saikat Bag, Arun Torris, E. Bhoje Gowd, Hasnain Sajid, Matthew A. Addicoat, Supratim Datta, Rahul Banerjee
Summary: In this study, a hierarchical TpAzo COF-foam with micro-, meso-, and macroporosity was synthesized using an in situ CO2 gas effervescence technique. The resulting TpAzo foam matrix showed higher immobilization efficiency for enzymes compared to the COF. The enzyme-immobilized TpAzo foam exhibited activity and stability, and was successfully utilized for a one-pot tandem conversion reaction with high recyclability.
Article
Engineering, Environmental
Jian Peng, Enhui Wu, Xiaoxuan Lou, Qian Deng, Xiaohong Hou, Chongning Lv, Qi Hu
Summary: In this study, a MOF/PVA/Lac composite with excellent enzymatic properties for degrading AQs and removing alizarin green efficiently was synthesized. The degradation product p-toluidine was identified using molecular docking, mass spectrometry, and 2DNMR, confirming the reaction mechanism C-alpha oxidation.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Rongrong Yuan, Yujie He, Bo Tang, Hongming He
Summary: Uniform spherical covalent organic frameworks (COFs) were synthesized at room temperature and used to immobilize horseradish peroxidase (HRP) with high loading capacity. The resulting HRP@COF displayed excellent reusability and enhanced tolerance to extreme conditions.
Review
Chemistry, Physical
Lucy C. Ward, Hannah V. McCue, Andrew J. Carnell
Summary: The review focuses on the potential use of carboxyl methyltransferases (CMTs) in industrial biotechnology, highlighting their ability to generate diverse methyl esters under aqueous conditions. Strategies for regenerating the required cofactor S-adenosylmethionine (SAM) are discussed, which will further improve the industrial applications of CMTs.
Article
Chemistry, Multidisciplinary
Xin Zhang, Jing Guo, Yue Yu, Xinmin Hao, Fucheng Guan, Sen Zhang
Summary: A cleaning and reusable biochemical degumming method using calcium alginate microspheres loaded with pectinase lyase (CA-PL) was proposed in this study. The method achieved complete degumming in a shorter time and at a lower temperature. The results showed that the biochemical degumming solution could be reused three times with high cellulose extraction rates and breaking strength. Repeating the method two times was considered optimal for better degumming efficiency and cellulose extraction degree.
NEW JOURNAL OF CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Xue Er Crystal Thew, Sewn Cen Lo, Ramakrishnan Nagasundara Ramanan, Beng Ti Tey, Nguyen Duc Huy, Ooi Chien Wei
Summary: Plastic biodegradation is seen as a sustainable and environmentally friendly solution to the growing problem of plastic waste. While there have been advancements in the biodegradation of certain types of plastics, there is still ongoing research focused on finding new microorganisms and enzymes for more effective and complete biodegradation. This review highlights various strategies to improve plastic biodegradation, including pretreatment methods and the use of additives. The challenges and future prospects in the field of plastic biodegradation are also discussed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Yingjun Cai, Yuqiu Chen, Haitao Zhang, Nicolas von Solms, Georgios M. Kontogeorgis, John M. Woodley, Kaj Thomsen
Summary: A computer-aided ionic liquid design method was used to screen and identify suitable ionic liquids for use as additives in electrolytes. A new type of ionic liquid was synthesized and tested in LTO/Li batteries, showing improved specific capacity and rate properties.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Review
Biotechnology & Applied Microbiology
Alexander Virklund, Sheila I. Jensen, Alex T. Nielsen, John M. Woodley
Summary: This article presents recent advances in genetic engineering and bioprocess concepts for the production of phenylpropanoids, including strategies such as adaptive laboratory evolution, enzyme engineering, in-situ product removal, and biocatalysis. The pros and cons of bacterial and yeast hosts for phenylpropanoid production are discussed.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Physical
Helena D. D. Tjornelund, Jesper Brask, John M. M. Woodley, Gunther H. J. Peters
Summary: In this study, an optimized protocol for controlling the initial water activity in organic reaction mixtures with soluble lipase was proposed. The protocol ensures reproducible and consistent enzyme activity measurements. The importance of consistent stirring and volume correction to compensate for solvent evaporation was highlighted. The study also tested the protocol on soluble and immobilized lipase and found similar water activity profiles and the highest enzyme activity in hexane. This optimized protocol expands the possibilities for studying enzyme kinetics.
Review
Chemistry, Physical
Julie Osterby Madsen, John M. M. Woodley
Summary: In vitro transaminase catalyzed asymmetric synthesis has been studied for some time, and the current challenge lies in downstream processing to recover the produced amine.
Article
Biotechnology & Applied Microbiology
Carlos Domingo-Felez, Katarzyna Jankowska, Ioannis V. Skiadas, John M. Woodley, Manuel Pinelo
Summary: Continuous glucose fermentation with ultrasonic sedimentation of yeast cells achieves higher bioethanol production rates. The efficiency of the ultrasonic system for cell separation was predicted and validated. The results showed that ultrasonic sedimentation improves cell retention and ethanol production efficiency.
FOOD AND BIOPRODUCTS PROCESSING
(2023)
Article
Biotechnology & Applied Microbiology
Angela V. Ruales-Salcedo, Oscar Andres Prado-Rubio, Javier Fontalvo, Juan Carlos Higuita, John M. Woodley
Summary: In this study, a kinetic model of the glucose oxidase (GOx) and catalase (CAT) system was established and validated through experiments. The results showed that the highest oxygen transport rate was achieved with the combined system of buffer, antifoam, GOx, and CAT. The kinetic models for the individual enzymes were accurate and identifiable. The model evaluation provided insights into the system's phenomenology. Rating: 8 points
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Simone Lauterbach, Hannah Dienhart, Jan Range, Stephan Malzacher, Jan-Dirk Spoering, Doerte Rother, Maria Filipa Pinto, Pedro Martins, Colton E. Lagerman, Andreas S. Bommarius, Amalie Vang Host, John M. Woodley, Sandile Ngubane, Tukayi Kudanga, Frank T. Bergmann, Johann M. Rohwer, Dorothea Iglezakis, Andreas Weidemann, Ulrike Wittig, Carsten Kettner, Neil Swainston, Santiago Schnell, Juergen Pleiss
Summary: The design of biocatalytic reaction systems is complex due to the dependency of kinetic parameters on the enzyme, reaction conditions, and modeling method. Enzymatic experiments and data reusability are challenging, leading to the development of EnzymeML, an XML-based markup language for storing and exchanging enzymatic data. EnzymeML facilitates the communication between experimental platforms, lab notebooks, modeling tools, publication platforms, and enzymatic reaction databases.
Article
Chemistry, Applied
Thomas Hilberath, Remco van Oosten, Juliet Victoria, Hugo Brasselet, Miguel Alcalde, John M. Woodley, Frank Hollmann
Summary: This study reports the mol-scale oxyfunctionalization of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using an unspecific peroxygenase. By utilizing AaeUPO from Agrocybe aegerita and simple H2O2 as an oxidant, cyclohexanol concentrations of more than 300 mM (>60% yield) with attractive productivities (157 mM h(-1), approx. 15 g L-1 h(-1)) were achieved. The current limitations of the proposed biooxidation system have been identified, paving the way for future improvements and implementation.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Article
Chemistry, Applied
Jingyu Wang, Elif Erdem, John M. Woodley
Summary: Biocatalytic oxidation holds promise for the selective synthesis of active pharmaceutical intermediates. Bubbling air or oxygen is an effective method to enhance oxygen transfer, but it leads to enzyme deactivation. This study investigates the stability of NAD-(P)H oxidase (NOX) at different gas-liquid interfaces and provides insights for improving biocatalyst performance.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Editorial Material
Chemistry, Multidisciplinary
Frank Hollmann, John M. Woodley
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Engineering, Chemical
Jinxin Fan, Sobhana A. Sripada, Dan N. Pham, Marina Y. Linova, John M. Woodley, Stefano Menegatti, Cristiana Boi, Ruben G. Carbonell
Summary: A high-capacity, multimodal cation exchange chromatographic membrane (MMC-MPCA) was developed using a polybutylene terepthalate (PBT) nonwoven fabric conjugated with 2-mercaptopyridine-3-carboxylic acid (MPCA) ligand. The membrane exhibited significantly higher binding capacities and clearance of impurities compared to commercial multimodal resins. It was also effective in protein elution under mild conditions. Proteomics analysis confirmed the clearance of high-risk impurities from the protein A eluate when using the MMC-MPCA membrane.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Deborah E. Carberry, Khosrow Bagherpour, John M. Woodley, Christian Beenfeldt, Martin P. Andersson, Ulrich Kruhne, Seyed Soheil Mansouri
Summary: This study designs and develops a virtual reality product to support teaching and learning in chemical engineering. By analyzing learning objectives and measuring resources, it identifies the opportunity to cultivate students' basic skills using virtual reality solutions in undergraduate courses. It proposes a strategic plan for developing virtual reality teaching tools for experiment-based courses.
DIGITAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Chemical
Deborah E. Carberry, Khosrow Bagherpour, Christian Beenfeldt, John M. Woodley, Seyed Soheil Mansouri, Martin P. Andersson
Summary: This paper explores the impact of shifting from paper-based to XR teaching tools through a design project, analyzing the cognitive and instructional aspects.
DIGITAL CHEMICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Helena D. Tjornelund, Jesper Vind, Jesper Brask, John M. Woodley, Gunther H. J. Peters
Summary: This study investigates the molecular mechanism of the specific activity of Candida antarctica lipase B (CALB) in low-water environments using molecular dynamics simulations. Based on experiments and simulations, four criteria influencing the performance of the enzyme in organic media were proposed and it was shown that specific mutants can resist changes in water activity.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Chemical
Sobhana A. Sripada, Driss Elhanafi, Leonard B. Collins, Taufika I. Williams, Marina Y. Linova, John M. Woodley, Cristiana Boi, Stefano Menegatti
Summary: This study presents the development and validation of a novel adsorbent, PichiaGuard, which is functionalized with peptide ligands and targets the whole spectrum of K. phaffii HCPs for protein purification. The PichiaGuard adsorbent has high HCP binding capacity and successfully purified monoclonal antibodies and fragments, showing its potential for modern biopharmaceutical processing.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)