Review
Biochemistry & Molecular Biology
Kim Thomsen, Niels Hoiby, Peter Ostrup Jensen, Oana Ciofu, Claus Moser
Summary: Biofilm infections are resistant to host responses and antibiotics. The host immune response plays a crucial role in the outcome and exacerbation of chronic infections. In chronic Pseudomonas aeruginosa lung infections, antibody response and Th1/Th2 imbalance have been associated with a worse outcome.
Article
Microbiology
Hernan F. Penaloza, Tolani F. Olonisakin, William G. Bain, Yanyan Qu, Rick van der Geest, Jill Zupetic, Mei Hulver, Zeyu Xiong, Michael W. Newstead, Chunbin Zou, Jonathan K. Alder, Joel A. Ybe, Theodore J. Standiford, Janet S. Lee
Summary: IL-36γ is a proinflammatory cytokine that requires proteolytic activation, while TSP-1 limits this activation by inhibiting extracellular proteolysis. TSP-1 deficiency leads to exaggerated inflammation during P. aeruginosa infection.
Article
Medicine, Research & Experimental
Lei Han, Yuning Huang, Qiang Fu, Yishu Xue, Fengming Ding, Min Zhang
Summary: Pseudomonas aeruginosa infection contributes to the progression of COPD, as an early Th1-related inflammation gradually shifts to a later Th2-related inflammation, and small-airway function decline occurs earlier than that of large-airway function.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Immunology
Qian Li, Hongyan Wang, Lisi Tan, Shuwei Zhang, Li Lin, Xiaolin Tang, Yaping Pan
Summary: The study revealed that the oral pathogen F. nucleatum could synergistically invade pulmonary epithelial cells with P. aeruginosa, leading to increased inflammation and altered cytotoxicity. This coaggregation may contribute to the deterioration of lung function in COPD patients with P. aeruginosa and F. nucleatum coinfection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Natalia Rustetska, Magdalena Szczepaniak, Krzysztof Goryca, Elwira Bakula-Zalewska, Malgorzata Figat, Artur Kowalik, Stanislaw Gozdz, Magdalena Kowalewska
Summary: This study identified tumour-promoting bacteria in vulvar squamous cell carcinoma (VSCC), which are associated with neutrophil recruitment and cancer progression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Immunology
Anne-Charlotte Jonckheere, Sven F. Seys, Brecht Steelant, Tatjana Decaesteker, Kaat Dekoster, Jonathan Cremer, Ellen Dilissen, Dominique Schols, Yoichiro Iwakura, Greetje Vande Velde, Christine Breynaert, Rik Schrijvers, Jeroen Vanoirbeek, Jan L. Ceuppens, Lieven J. Dupont, Dominique M. A. Bullens
Summary: This study developed a murine model for non-allergic neutrophilic asthma and found that ILC play a key role in inducing airway hyperreactivity. ILC are also associated with inflammatory response and cytokine release.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Raquel Lopez-Galvez, Isabelle Fleurot, Pablo Chamero, Sascha Trapp, Michel Olivier, Claire Chevaleyre, Celine Barc, Mickael Riou, Christelle Rossignol, Antoine Guillon, Mustapha Si-Tahar, Tobias May, Pascal Barbry, Andrea Bahr, Nikolai Klymiuk, Jean-Claude Sirard, Ignacio Caballero
Summary: Research using a pig model demonstrated that stimulation of the TLR5 receptor by flagellin can modulate NF-kappa B signaling and intracellular calcium homeostasis, reducing inflammation in inflammatory lung diseases. This finding suggests flagellin treatment may help regulate the inflammatory response in diseases like cystic fibrosis.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Microbiology
Lisa Juliane Kahl, Kelly N. Eckartt, Diana K. Morales, Alexa Price-Whelan, Lars E. P. Dietrich
Summary: Organisms that do not obtain energy from light can be affected by daily changes in light exposure. This study found that light/dark and temperature cycling have lasting effects on redox metabolism in biofilms formed by a nonphototrophic, pathogenic bacterium.
Article
Biochemistry & Molecular Biology
Marko Nedeljkovic, Sandra Postel, Brian G. Pierce, Eric J. Sundberg
Summary: The study identified the D1 domain of FliD as crucial for functional flagella formation, with the head region showing good tolerance to sequences from different bacterial species. Additionally, head domains can be replaced by small peptides or larger head domains from different species and still produce functional flagella.
Article
Microbiology
Ayesha Z. Beg, Faraz Rashid, Absar Talat, Mohd Azam Haseen, Nadeem Raza, Kafil Akhtar, Morten Kam Dahl Dueholm, Asad U. Khan
Summary: The Fap operon of Pseudomonas aeruginosa plays a crucial role in pathoadaptation during pulmonary infection. Its presence increases pathogenesis and biofilm sustainability by modulating bacterial physiology. Targeting amyloids could be a potential clinical application to exploit the pathoadaptive role of Fap in pulmonary infections. The genetic conservation and extracellular exposure of Fap make it a commendable target for interventions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Ananya Datta, Justin Lee, Tiffany Truong, David J. Evans, Suzanne M. J. Fleiszig
Summary: This study examined the impact of ocular-associated bacteria on corneal cell responses during contact lens wear. The results showed that antibiotic treatment reduced CD11c+ cell numbers and suppressed cell responses to lens wear. The presence of some bacteria played a role in maintaining baseline cell populations and lens-associated cell responses.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Nina Pennetzdorfer, Medeea C. Popescu, Naomi L. Haddock, Fannie Dupuy, Gernot Kaber, Aviv Hargil, Patrik K. Johansson, Annika Enejder, Paul L. Bollyky
Summary: Pseudomonas aeruginosa, a major human pathogen, can effectively colonize the airways of patients with cystic fibrosis. This study found that the bacteriophage Pf4 produced by P. aeruginosa modifies the innate immune response to P. aeruginosa infections via TLR3 signaling. The phage dampens inflammatory responses to bacterial endotoxin by attaching to bacterial vesicles, potentially reducing inflammation at sites of bacterial colonization or infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Immunology
Pamella Constantino-Teles, Albane Jouault, Lhousseine Touqui, Alessandra Mattos Saliba
Summary: Pseudomonas aeruginosa, an opportunistic pathogen, causes respiratory infections by utilizing various virulence factors, such as bacterial lipids and enzymes, and by interacting with host lipids. These mechanisms play important roles in the establishment and persistence of P. aeruginosa in the lungs, as well as in modulating host immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Henning Carstens, Katharina Kalka, Rabea Verhaegh, Fabian Schumacher, Matthias Soddemann, Barbara Wilker, Simone Keitsch, Carolin Sehl, Burkhard Kleuser, Thorsten Wahlers, Gerald Reiner, Achim Koch, Ursula Rauen, Erich Gulbins, Markus Kamler
Summary: Inhalation of sphingosine increases sphingosine concentrations in the luminal plasma membrane of tracheal and bronchial epithelial cells, with no local side effects in ex-vivo perfused and ventilated minipig lungs.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Tong Wu, Annie Gagnon, Katherine McGourty, Rebecca DosSantos, Lucia Chanetsa, Boce Zhang, Dhimiter Bello, Shannon L. Kelleher
Summary: Excessive zinc exposure significantly enhances various virulence mechanisms of P. aeruginosa, promoting its transition from commensal to pathogen and resulting in illness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pediatrics
Chhinder P. Sodhi, Peter Wipf, Yukihiro Yamaguchi, William B. Fulton, Mark Kovler, Diego F. Nino, Qinjie Zhou, Emilyn Banfield, Adam D. Werts, Mitchell R. Ladd, Rachael H. Buck, Karen C. Goehring, Thomas Prindle, Sanxia Wang, Hongpeng Jia, Peng Lu, David J. Hackam
Summary: The study showed that human milk oligosaccharides 2'-FL and 6'-SL can prevent NEC through inhibition of TLR4 signaling, while lactose cannot. The results indicate that these HMOs reduced NEC occurrence in mouse and piglet models.
PEDIATRIC RESEARCH
(2021)
Article
Cell Biology
Jiaxi Xu, Eric Lazartigues
Summary: The recent outbreak of COVID-19 caused by the novel coronavirus SARS-CoV-2 has spread rapidly worldwide. Similar to SARS-CoV, this new coronavirus utilizes ACE2 as an entry point to cells. Growing evidence suggests that COVID-19 patients, particularly those in severe condition, exhibit neurological symptoms, indicating the potential for SARS-CoV-2 to infect and damage neurons in the central nervous system. By studying human neurons derived from pluripotent stem cells, we have demonstrated the expression of ACE2 in human neurons. This highlights the importance of considering the neuro-invasive potential of SARS-CoV-2 as both a contributing factor and a therapeutic target for the severe respiratory symptoms in critical COVID-19 cases.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Blessing Ogunlade, Jessie J. Guidry, Snigdha Mukerjee, Srinivas Sriramula, Eric Lazartigues, Catalin M. Filipeanu
Summary: In this study, the researchers discovered that ACE2 is internalized and degraded in lysosomes after chronic Ang-II treatment. They identified fascin-1 as an accessory protein of ACE2 that interacts with the enzyme in an Ang-II dependent manner. Overexpression of fascin-1 attenuates the effects of Ang-II on ACE2 activity, while downregulation of fascin-1 severely decreases ACE2 enzymatic activity.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Pediatrics
Chhinder P. Sodhi, Peter Wipf, Yukihiro Yamaguchi, William B. Fulton, Mark Kovler, Diego F. Nino, Qinjie Zhou, Emilyn Banfield, Adam D. Werts, Mitchell R. Ladd, Rachael H. Buck, Karen C. Goehring, Thomas Prindle, Sanxia Wang, Hongpeng Jia, Peng Lu, David J. Hackam
PEDIATRIC RESEARCH
(2021)
Review
Medicine, Research & Experimental
Mona Elgazzaz, Eric Lazartigues
Summary: Cardiometabolic diseases are prevalent and high mortality diseases. The complexity of their etiology involves the intertwining of genetic and environmental factors through epigenetics, leading to diverse disease courses and outcomes among individuals. Targeting epigenetic imprints on genes like RAS can potentially impact the susceptibility to cardiometabolic dysfunction.
Article
Cardiac & Cardiovascular Systems
Juan Hong, Shubin Fu, Lie Gao, Yanhui Cai, Eric Lazartigues, Han-Jun Wang
Summary: The dysfunction of voltage-gated potassium (Kv) channels in dorsal root ganglions plays a critical role in mediating the exaggerated exercise pressor reflex and muscle afferent sensitization in chronic heart failure (CHF) patients. Manipulating Kv channels in DRG neurons could be a potential new approach to reduce sympathoexcitation and improve exercise intolerance in CHF, ultimately enhancing quality of life and reducing mortality.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Cell Biology
Mark L. Kovler, Andres J. Gonzalez Salazar, William B. Fulton, Peng Lu, Yukihiro Yamaguchi, Qinjie Zhou, Maame Sampah, Asuka Ishiyama, Thomas Prindle, Sanxia Wang, Hongpeng Jia, Peter Wipf, Chhinder P. Sodhi, David J. Hackam
Summary: Necrotizing enterocolitis (NEC) is a devastating disease in premature infants, with pathogenesis involving intestinal dysmotility, enteric glia loss, and TLR4 activation. Restoration of intestinal motility or enhancing enteric glial BDNF release can alleviate NEC severity.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Family Studies
Mark L. Kovler, Susan Ziegfeld, Leticia M. Ryan, Mitchell A. Goldstein, Rebecca Gardner, Alejandro Garcia, Isam W. Nasr
Summary: The study found an increase in the proportion of traumatic injuries caused by physical child abuse at the pediateric trauma center during the Covid-19 pandemic, indicating a need for thoughtful implementation of strategies to mitigate this secondary effect of social distancing.
CHILD ABUSE & NEGLECT
(2021)
Review
Gastroenterology & Hepatology
David J. Hackam, Chhinder P. Sodhi
Summary: Necrotizing enterocolitis (NEC) is a serious gastrointestinal disease that affects premature infants, causing high morbidity and mortality. Recent research has provided new insights into the pathogenesis of NEC and potential therapies.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2022)
Editorial Material
Pediatrics
David J. Hackam
Summary: This study suggests that anemia may increase the risk of necrotizing enterocolitis development through impaired tissue oxygenation, shifting the focus back onto the trigger for blood transfusion rather than the transfused blood itself.
PEDIATRIC RESEARCH
(2022)
Article
Cell Biology
Stephanie N. Langel, Susan Johnson, Clarissa Martinez, Sarah N. Tedjakusuma, Nadine Peinovich, Emery G. Dora, Philip J. Kuehl, Hammad Irshad, Edward G. Barrett, Adam D. Werts, Sean N. Tucker
Summary: Vaccination through oral or intranasal routes can generate strong antibody responses, reduce viral load in the nose and lungs, and mitigate lung pathology caused by SARS-CoV-2. Mucosally vaccinated hamsters transmitted fewer viruses with less severity to naive hamsters, suggesting that mucosal immunization can reduce viral transmission. In a clinical trial, the same platform also induced mucosal cross-reactive antibody responses.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Nicholas D. Fried, Anna Whitehead, Eric Lazartigues, Xinping Yue, Jason D. Gardner
Summary: This study using a bilateral ovariectomy model suggests that the cardiopulmonary protection observed in nicotine-exposed female mice may be independent of ovarian hormones.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Pediatrics
Mitchell R. Ladd, R. Ellen Jones, Ranim Alsaad, Samuel M. Alaish
Summary: This article presents a rare and important complication of central venous access in pediatric surgery, involving a calcified fibrin sheath embolizing into the right pulmonary artery system. The diagnosis was initially unclear, leading to multiple diagnostic studies and cardiac catheterization for final diagnosis, followed by successful treatment with anticoagulation.
JOURNAL OF PEDIATRIC SURGERY CASE REPORTS
(2022)
Article
Gastroenterology & Hepatology
Johannes W. W. Duess, Maame E. E. Sampah, Carla M. M. Lopez, Koichi Tsuboi, Daniel J. J. Scheese, Chhinder P. P. Sodhi, David J. J. Hackam
Summary: Necrotizing enterocolitis (NEC) is a devastating disease in premature infants, caused by dietary and bacterial factors in a vulnerable host. Intestinal perforation can lead to severe infection and sepsis. Toll-like receptor 4 has been identified as a critical regulator of NEC development. This review article presents recent findings on microbial signaling, the immature immune system, intestinal ischemia, systemic inflammation, and potential therapeutic approaches.