Article
Virology
Longxiang Zhang, Rui Li, Rui Geng, Lei Wang, Xin-Xin Chen, Songlin Qiao, Gaiping Zhang
Summary: Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses in the swine industry. This study identifies tumor susceptibility gene 101 (TSG101), an ESCRT component, as an important factor in PRRSV infection. TSG101 plays a role in virion formation and subcellular localization of PRRSV N protein along the early secretory pathway. These findings suggest that TSG101 is a potential target for interfering with PRRSV infection.
JOURNAL OF VIROLOGY
(2022)
Review
Plant Sciences
Erika Isono
Summary: ESCRTs, initially identified in yeast, play crucial roles in endosomal trafficking, cytokinesis, autophagy, viral budding, and other cellular events. Recent studies have highlighted their versatile functions in diverse organisms, emphasizing their significance in physiological processes.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Virology
Chun-chun Liu, Ya-yun Liu, Yan Cheng, Yun-Na Zhang, Jin Zhang, Xiao-Dong Liang, Ya Gao, Huan Chen, Abdul Sattar Baloch, Qian Yang, Yun Young Go, Bin Zhou
Summary: This study highlights the regulatory role of the ESCRT-I complex protein Tsg101 in the entry and replication processes of CSFV, suggesting that ESCRT plays a crucial role in the life cycle of the virus. Tsg101 may serve as a potential molecular target for developing pestivirus inhibitors.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Yukari Itakura, Koshiro Tabata, Takeshi Saito, Kittiya Intaruck, Nijiho Kawaguchi, Mai Kishimoto, Shiho Torii, Shintaro Kobayashi, Naoto Ito, Michiko Harada, Satoshi Inoue, Ken Maeda, Ayato Takada, William W. Hall, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki
Summary: The interaction between the structural protein of the Ebola virus and the host TSG101 protein plays a significant role in the replication and spread of the virus.
JOURNAL OF VIROLOGY
(2023)
Review
Virology
Kevin M. Rose
Summary: Proper assembly and dissemination of progeny virions is crucial for virus replication, with viruses evolving various strategies to exploit cellular mechanisms for successful infection. Different viruses, such as retroviruses and herpesviruses, utilize host ESCRT complexes in distinct ways to ensure viral particle formation. Despite occurring in separate subcellular compartments and at different steps, the role of ESCRTs in viral lifecycles appears to be conserved.
Review
Oncology
Ye Yang, Min Wang, Ying-Ying Zhang, Shu-Zhi Zhao, Song Gu
Summary: ESCRT plays a crucial role in cell death and autophagy by repairing damaged plasma membranes and removing pores, thus preserving cell survival. Additionally, ESCRT is involved in overcoming drug resistance during tumor therapy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biophysics
Lijuan Yin, Shuli Man, Shengying Ye, Guozhen Liu, Long Ma
Summary: This article highlights the recent advances in virus detection using CRISPR-Cas systems, particularly CRISPR-Cas12a/Cas13a. These systems, known for their sensitivity, specificity, high base resolution, and programmability, are revolutionizing virus detection and offering new possibilities in the field.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Yichen Ju, Haocheng Bai, Linzhu Ren, Liying Zhang
Summary: The ESCRT system is composed of multiple protein complexes, playing important roles in the degradation of plasma membrane proteins, biogenesis of lysosomes and vacuoles, enveloped virus budding, and cytokinesis. Recent studies have shown the significance of exosomes and the ESCRT pathway in virus infections, providing valuable insights for understanding the infection mechanisms of other enveloped viruses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Genetics & Heredity
Muntazir Mushtaq, Aejaz Ahmad Dar, Umer Basu, Basharat Ahmad Bhat, Rakeeb Ahmad Mir, Sanskriti Vats, M. S. Dar, Anshika Tyagi, Sajad Ali, Monika Bansal, Gyanendra Kumar Rai, Shabir Hussain Wani
Summary: Plant pathology has been revolutionized by next-generation sequencing technologies, enabling fast, cost-effective, and reliable diagnostics for pathogens. NGS has advanced research and diagnostics of plant infecting viromes, bridging plant virology with genome editing technologies, and holds potential for improving plant health. Integration of NGS with CRISPR-based genome editing technologies is poised to revolutionize combating deadly plant viruses at the genomic level.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Alberto Blanch Jover, Cees Dekker
Summary: The Cdv system performs cell division and membrane-deforming processes in certain archaea and is evolutionarily related to the eukaryotic ESCRT machinery. Recent discoveries have renewed interest in the Cdv system and shed light on its molecular mechanisms. This paper provides a comprehensive overview of the research on the Cdv system and its cell-division function, offering insights into biophysics, evolution, and synthetic cell engineering.
TRENDS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Zainul A. Khan, Rohit Kumar, Indranil Dasgupta
Summary: This review discusses the application of CRISPR/Cas9 in antiviral research, including strategies targeting viral genomes and host factors, as well as the use of base editing to generate transgene-free virus-resistant plants. The review also compares the efficiencies of different approaches and discusses how to increase editing efficiency and bypass the generation of escape mutants through combinatorial approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Sajad Najafi, Shing Cheng Tan, Shahin Aghamiri, Pourya Raee, Zahra Ebrahimi, Zahra Kargar Jahromi, Yazdan Rahmati, Javid Sadri Nahand, Ahmad Piroozmand, Vahid Jajarmi, Hamed Mirzaei
Summary: The article reviews the research progress of CRISPR-Cas technology in treating viral infections, discusses delivery strategies and challenges, and proposes future research directions.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Nebojsa Jukic, Alma P. Perrino, Frederic Humbert, Aurelien Roux, Simon Scheuring
Summary: In this study, the authors used high-speed AFM to investigate the characteristics of Snf7, the major component of ESCRT-III, on nano-patterned and soft supports. They found that loaded Snf7 spiral springs are curvature sensitive and deform membranes.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Sai Sudha Mannemuddhu, Huanzhou Xu, Christopher K. E. Bleck, Nico Tjandra, Carol Carter, Sumita Bhaduri-McIntosh
Summary: Three structurally distinct prazoles were found to impair EBV capsid maturation and virion transport from the nucleus, preventing EBV release. These findings implicate Tsg101 in EBV production and suggest prazoles as potential candidates for preventing posttransplant EBV lymphomas.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Wenyuan Shen, Chang Liu, Yue Hu, Qian Ding, Jiabin Feng, Zhou Liu, Xiaohong Kong
Summary: This study found that the replication of human immunodeficiency virus-1 (HIV-1) is inhibited when the level of spastin protein in cells is reduced, because spastin enhances virus production by promoting the lysosomal degradation of the Gag protein. Further investigation revealed that increased sodium tolerance 1 (IST1), a subunit of the endosomal sorting complex required for transport (ESCRT), can interact with the MIT domain of spastin to regulate the production of intracellular Gag. In summary, spastin is crucial for HIV-1 replication, and the interaction between spastin and IST1 facilitates virus production by regulating the intracellular trafficking and degradation of Gag protein. Therefore, spastin may serve as a new target for HIV-1 prophylactic and therapy.
Editorial Material
Virology
Melissa V. Fernandez, Krista A. Delviks-Frankenberry, David A. Scheiblin, Christine Happel, Vinay K. Pathak, Eric O. Freed
JOURNAL OF VIROLOGY
(2019)
Article
Multidisciplinary Sciences
Nairi Pezeshkian, Nicholas S. Groves, Schuyler B. van Engelenburg
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Virology
Melissa V. Fernandez, Huxley K. Hoffman, Nairi Pezeshkian, Philip R. Tedbury, Schuyler B. van Engelenburg, Eric O. Freed
JOURNAL OF VIROLOGY
(2020)
Article
Microbiology
Nicholas S. Groves, Merissa M. Bruns, Schuyler B. van Engelenburg
Article
Cell Biology
Paulius Gibieza, Eric Peterman, Huxley K. Hoffman, Schuyler Van Engeleburg, Vytenis Arvydas Skeberdis, Rytis Prekeris
Summary: The study identified Rab14 as a novel regulator of cytokinesis that contributes to the efficiency of recruiting Rab11-endosomes to the thin intracellular bridge (ICB) microtubules and affects actin clearance at the abscission site. Furthermore, Rab14 was found to bind to microtubule minus-end interacting MACF2/CAMSAP3 complex, influencing the targeting of endosomes to the ICB microtubules during cytokinesis.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Virology
Xenia Snetkov, Tafhima Haider, Dejan Mesner, Nicholas Groves, Schuyler B. van Engelenburg, Clare Jolly
Summary: The W757 amino acid motif in the HIV-1 envelope was found to play a key role in viral replication and spread between T cells. Mutation at this position inhibits cell-cell spread and affects virological synapse formation and viral fusion. The W757 motif regulates the recruitment of Env to virus assembly sites and the fusogenicity of the Env ectodomain, thus supporting efficient HIV-1 replication and spread.
Review
Cell Biology
Huxley K. Hoffman, Rytis Prekeris
Summary: Primary cilia are important for cells to respond to extracellular stimuli. Recent research has shed light on the regulation of ciliogenesis in different cell types. Actin cytoskeleton dynamics play a crucial role in various stages of ciliogenesis. Additionally, studies have revealed the requirements for actin remodeling in the formation of motile cilia in multiciliated epithelial cells, suggesting an alternative ciliogenesis pathway.
JOURNAL OF CELL SCIENCE
(2022)
Article
Virology
Huxley K. Hoffman, Rebekah S. Aguilar, Austin R. Clark, Nicholas S. Groves, Nairi Pezeshkian, Merissa M. Bruns, Schuyler B. van Engelenburg
Summary: This study investigates the trafficking pathways of internalized HIV-1 envelope glycoprotein (Env) in infected CD4(+) T cells and the role of host cell Rab GTPases. The findings suggest that internalized Env traffics to Rab14(+) compartments that resemble late endosomes and lysosomes. Additionally, the study demonstrates that Env can be recycled back to the plasma membrane.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Melissa V. Fernandez V. de Cespedes, Huxley K. Hoffman, Hannah Carter, Lacy M. Simons, Lwar Naing, Sherimay D. Ablan, David A. Scheiblin, Judd F. Hultquist, Schuyler B. van Engelenburg, Eric O. Freed
Summary: This study investigates the role of FIP1C in HIV-1 Env incorporation and virus replication in different human T-cell types. The results suggest that while FIP1C may contribute to Env incorporation in some contexts, it is not essential for efficient HIV-1 replication in primary CD4(+) T cells.
JOURNAL OF VIROLOGY
(2022)