Review
Oncology
Ye Yang, Min Wang, Ying-Ying Zhang, Shu-Zhi Zhao, Song Gu
Summary: ESCRT plays a crucial role in cell death and autophagy by repairing damaged plasma membranes and removing pores, thus preserving cell survival. Additionally, ESCRT is involved in overcoming drug resistance during tumor therapy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Virology
Wenyuan Shen, Chang Liu, Yue Hu, Qian Ding, Jiabin Feng, Zhou Liu, Xiaohong Kong
Summary: This study found that the replication of human immunodeficiency virus-1 (HIV-1) is inhibited when the level of spastin protein in cells is reduced, because spastin enhances virus production by promoting the lysosomal degradation of the Gag protein. Further investigation revealed that increased sodium tolerance 1 (IST1), a subunit of the endosomal sorting complex required for transport (ESCRT), can interact with the MIT domain of spastin to regulate the production of intracellular Gag. In summary, spastin is crucial for HIV-1 replication, and the interaction between spastin and IST1 facilitates virus production by regulating the intracellular trafficking and degradation of Gag protein. Therefore, spastin may serve as a new target for HIV-1 prophylactic and therapy.
Review
Virology
Bo Meng, Andrew M. L. Lever
Summary: Viruses are obligate parasites that rely on host cellular factors for replication and spread, often hijacking host machinery such as the ESCRT system. Research on ESCRT and viruses has advanced our understanding of various cellular functions, with a special focus on the interplay between the ESCRT pathway and enveloped viruses like retroviruses.
Review
Microbiology
Chunxuan Wang, Yu Chen, Shunlin Hu, Xiufan Liu
Summary: The endosomal sorting complex required for transport (ESCRT) is a molecular machinery in eukaryotic cells that facilitates membrane invagination and the formation of multivesicular bodies. It participates in various cellular processes and can be hijacked by enveloped viruses for their entry, replication, and budding. This review focuses on the interaction between ESCRT and different viruses.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Immunology
Cecilia Banuelos, Abigail Betanzos, Rosario Javier-Reyna, Ausencio Galindo, Esther Orozco
Summary: This study summarizes the research progress on the ESCRT machinery in E. histolytica, focusing on the role of ESCRT-I and ESCRT-III components and the EhADH and EhVps4-ATPase accessory proteins in phagocytosis. The study found that EhADH plays a multifunctional role in the endocytic pathway and interacts with EhVps32 to form a concurrent route for MVBs biogenesis. In addition, other components from the endosomal pathway are also secreted.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Cell Biology
Amra Saric, Spencer A. Freeman
Summary: Eukaryotic cells utilize diverse uptake mechanisms for internalizing extracellular solutes and fluid into membrane-bound endosomes. After internalization, the endosomes undergo extensive remodeling involving tubulation and vesiculation, followed by maturation through retrograde transport and fusion with late endosomes and lysosomes. Control of tension on endomembranes is crucial for sorting and trafficking, with the efflux of major solutes along the endocytic pathway playing a key role in regulating membrane tension.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Chemical
Mafalda G. Moleirinho, Sean Feast, Ana S. Moreira, Ricardo J. S. Silva, Paula M. Alves, Manuel J. T. Carrondo, Tim Huber, Conan Fee, Cristina Peixoto
Summary: Advancements in designing new biotherapeutic products using viral particles have been witnessed in recent decades, with the limitations of manufacturing these particles often related to purification processes. This study demonstrates the successful use of 3D-printed cellulose chromatographic columns functionalized with different ligands for the purification of oncolytic adenovirus and lentiviral vectors, achieving efficient separations with high recovery yields. The application of 3D printing technologies in viral vector manufacturing shows potential to improve downstream process efficiency.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Thermodynamics
Leonard F. Pease, Na Wang, Gourihar R. Kulkarni, Julia E. Flaherty, Carolyn A. Burns
Summary: This study evaluates the impact of mucus layers on the evaporation time and transmission of enveloped viruses. The findings suggest that enveloped viruses can remain infective over larger distances when surrounded by mucus shells. This has significant implications for public health policies.
INTERNATIONAL COMMUNICATIONS IN HEAT AND MASS TRANSFER
(2022)
Review
Biochemistry & Molecular Biology
Sunandini Chandra, C. Patrick Lusk
Summary: Studies have found that repeat expansions in the C9ORF72 gene are associated with ALS and may cause pathogenesis by disrupting nuclear transport machinery. Recent research has revealed changes in the molecular composition of the nuclear pore complex in patient neurons and dysregulation of quality control mechanisms at the nuclear envelope, which may contribute to the development of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Kai-En Chen, Qian Guo, Timothy A. Hill, Yi Cui, Amy K. Kendall, Zhe Yang, Ryan J. Hall, Michael D. Healy, Joanna Sacharz, Suzanne J. Norwood, Sachini Fonseka, Boyang Xie, Robert C. Reid, Natalya Leneva, Robert G. Parton, Rajesh Ghai, David A. Stroud, David P. Fairlie, Hiroaki Suga, Lauren P. Jackson, Rohan D. Teasdale, Toby Passioura, Brett M. Collins
Summary: The retromer complex plays a crucial role in endosomal membrane trafficking and signaling. Novel macrocyclic peptides have been developed to modulate its function by specifically binding to different subunits and disrupting interactions with other factors. These peptides provide insights into retromer function and offer a new therapeutic avenue for targeting this complex.
Article
Oncology
Jianguo Wang, Yan Liu, Yuanbin Zhang, Xiaoyang Li, Min Fang, Dong Qian
Summary: This study investigated the function and mechanism of a targeting exosome system (iRGD-exo-antagomiR) against vasculogenic mimicry (VM) and angiogenesis in nasopharyngeal carcinoma (NPC) and provided new approaches for NPC treatment.
Review
Biochemistry & Molecular Biology
America Chandia Cristi, Sampath Rapuri, Alyssa N. Coyne
Summary: Nuclear pore complexes (NPCs) are crucial for maintaining nuclear-cytoplasmic balance and proper nuclear organization, and perturbations in their regulation are implicated in neurodegenerative diseases. The ESCRT-III pathway plays a critical role in the surveillance and preservation of functional NPCs and nuclear envelope sealing. Maintaining quality control of the nuclear envelope and NPCs is pivotal in the pathogenesis of neurodegenerative diseases.
Article
Medicine, General & Internal
Yu Guo, Jian Shi, Zeyun Zhao, Min Wang
Summary: Pan-cancer analysis showed that CHMP7 expression is correlated with clinical prognosis, DNA methylation, protein phosphorylation, and immune cell infiltration, providing new ideas or targets for diagnosis or treatment. CHMP7 is involved in the composition of ESCRT complexes and functions in binding to GTP, exerting GTPase activity, and promoting multivesicular bodies assembly, with main pathways expressed in endocytosis, gap junction, and phagosome.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2021)
Article
Immunology
Lucia Barrado-Gil, Isabel Garcia-Dorival, Inmaculada Galindo, Covadonga Alonso, Miguel angel Cuesta-Geijo
Summary: The African swine fever virus relies on the endocytic pathway and cellular membrane remodeling for its infection. ESCRT-related proteins, VPS4A and ALIX, play crucial roles in ASFV replication and viral production. Furthermore, the unconventional phospholipid LBPA and its interaction with ALIX affect ASFV infection at both early and late stages of the viral cycle.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
K. Imai, A. Hagi, S. Yamagawa, D. Sano
Summary: This study demonstrated the potential of olanexidine gluconate formulations as an environmental disinfectant against enveloped viruses, showing immediate virucidal activity and long-lasting efficacy on environmental surfaces.
JOURNAL OF HOSPITAL INFECTION
(2021)
Correction
Infectious Diseases
Linzhu Ren, Chun Li, Yanling Yang
INFECTION GENETICS AND EVOLUTION
(2021)
Letter
Multidisciplinary Sciences
Si Chen, Liying Zhang, Lin Wang, Hongsheng Ouyang, Linzhu Ren
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Lin Yang, Xiaohua Liu, Liying Zhang, Xue Li, Xinwei Zhang, Guyu Niu, Weilong Ji, Si Chen, Hongsheng Ouyang, Linzhu Ren
Summary: The study indicates that porcine TRIM21 plays a critical role in enhancing PCV2 infection by increasing interferons and proinflammatory factors while decreasing cellular apoptosis.
Review
Virology
Weijuan Jia, Si Chen, Shanshan Chi, Yunjiang He, Linzhu Ren, Xueli Wang
Summary: This paper reviews recent progress on tick-borne bacterial diseases, viral diseases, and parasitic diseases in China, emphasizing the significance of research on ticks and tick-borne diseases.
Article
Biochemistry & Molecular Biology
Xue Li, Si Chen, Guyu Niu, Xinwei Zhang, Weilong Ji, Ying Ren, Liying Zhang, Linzhu Ren
Summary: This study analyzed the genetic relationship between PCV4 and other circoviruses, and found that PCV4 is more similar to MiCV, suggesting a potential common origin or derivation from MiCV. This raises concerns about the potential threat of PCV4 to animals and even humans. Therefore, continuous monitoring of PCV4 infection and variation, as well as the establishment of prevention and treatment methods, is necessary.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xue Li, Si Chen, Liying Zhang, Guyu Niu, Xinwei Zhang, Lin Yang, Weilong Ji, Linzhu Ren
Summary: Coinfection of PCV2 and PRV can enhance the activation of immune and inflammatory pathways, promoting the expression of interferons and interleukins while inhibiting other immune pathways. These results contribute to understanding the immune escape mechanisms of PCV2 and PRV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Si Chen, Xue Li, Xinwei Zhang, Guyu Niu, Lin Yang, Weilong Ji, Liying Zhang, Linzhu Ren
Summary: This study found that PRV inhibits PCV2 proliferation at 36 to 72 hours post-infection, while PCV2 enhances PRV proliferation in the middle stage of infection. PRV is the main factor during coinfection. Coinfection with PCV2 and PRV activates the ERS pathway and upregulates the expression of related proteins, leading to increased cell apoptosis and exacerbation of diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Guyu Niu, Xinwei Zhang, Weilong Ji, Si Chen, Xue Li, Lin Yang, Liying Zhang, Hongsheng Ouyang, Chang Li, Linzhu Ren
Summary: In this study, PCV4 was successfully rescued and shown to be pathogenic in pigs. Visible pathological changes were observed in the inoculated piglets, and effective humoral and cellular immune responses were stimulated.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Review
Virology
Guyu Niu, Si Chen, Xue Li, Liying Zhang, Linzhu Ren
Summary: This review summarizes the latest research progress on the molecular mechanism of porcine circoviruses (PCVs)-host interaction, discussing their pathogenicity and infection mechanism, which provides a scientific basis for disease prevention and control.
Article
Biochemistry & Molecular Biology
Xue Li, Si Chen, Liying Zhang, Jiawei Zheng, Guyu Niu, Lin Yang, Xinwei Zhang, Linzhu Ren
Summary: This study compared the amino acid sequences and spatial conformation of proteins, and evaluated the interactions between the virus and host proteins. The results shed light on the impact of viral variants on immune surveillance and the potential mechanism of cross-species transmission.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Xinwei Zhang, Si Chen, Xue Li, Liying Zhang, Linzhu Ren
Summary: Flavonoids are natural substances with phenolic structures that have antioxidant, anti-inflammatory, anticancer, and antiviral activities. This review summarizes the recent progress in understanding the antiviral mechanisms of flavonoids against porcine viruses, highlighting their potential for controlling porcine virus infection and developing pig feed additives.
Review
Biochemistry & Molecular Biology
Xue Li, Lin Yang, Si Chen, Jiawei Zheng, Huimin Zhang, Linzhu Ren
Summary: TRIM21, a member of the TRIM family, possesses an E3 ubiquitin ligase activity. Recent studies have shown that TRIM21 is involved in virus infection by interacting with viral proteins or modulating immune and inflammatory responses. TRIM21 also acts as a cytosol high-affinity antibody Fc receptor, triggering antibody-dependent intracellular neutralization (ADIN).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Virology
Hongsheng Ouyang, Linzhu Ren
Article
Microbiology
Si Chen, Xue Li, Liying Zhang, Jiawei Zheng, Lin Yang, Guyu Niu, Huimin Zhang, Ying Ren, Jing Qian, Changjiang Sun, Linzhu Ren
Summary: This study analyzed 302 samples collected in the Jilin Province of China from 2016 to 2021 and investigated the genotypes and genetic characteristics of PCV2 isolates. The predominant genotypes of PCV2 in Jilin Province were found to be PCV2b, followed by PCV2e and PCV2d. Mutations were detected in the isolates, but no recombination occurred, indicating a stable genotype of PCV2 in Jilin Province. The study also highlighted changes in antigen epitopes and amino acid mutations compared to currently used vaccine strains.
Review
Biochemistry & Molecular Biology
Yuqi Wang, Linzhu Ren, Haocheng Bai, Qing Jin, Liying Zhang
Summary: Exosomes, extracellular vesicles (EVs) in bodily fluids, play important roles in various physiological processes. The interaction between autophagy and endosomal/exosomal pathways has significant influences on normal physiology and human diseases. The interplay between exosomes and the autophagy pathway is complex, with exosomes inducing intracellular autophagy and autophagy modulating the biogenesis and degradation of exosomes. They also contribute to the infection process of enveloped viruses by encapsulating viral components and transferring them between cells. This review provides valuable insights into the interplay between exosomes and autophagy, as well as their implications in enveloped virus infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
