Article
Multidisciplinary Sciences
Bowen Wang, Zhiheng Zhao, Michael Xiong, Rui Yan, Ke Xu
Summary: The endoplasmic reticulum (ER) is a versatile organelle with distinct forms of tubules, regulated by Rtn4 and Climp63, that together form a ribbon-like structure of well-defined width.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Cell Biology
Hana Popelka, Daniel J. J. Klionsky
Summary: A recent study used cryo-electron tomography combined with computational analysis to gain insights into autophagosome biogenesis in yeast cells. This approach provided new information on autophagic structures, their contacts with organelles, membrane sources, and transition mechanisms. These findings open new avenues for autophagy research and highlight the potential of cryo-ET in cell biology.
Review
Cell Biology
Chenlu Li, Tiantian Qian, Ruyue He, Chun Wan, Yinghui Liu, Haijia Yu
Summary: Endoplasmic reticulum (ER) forms direct membrane contact sites with the plasma membrane (PM) in eukaryotic cells, playing crucial roles in lipid homeostasis, ion dynamics, and cell signaling through protein-protein or protein-lipid interactions. Distinct tethering factors dynamically regulate the architecture of ER-PM junctions in response to signals, and the physiological roles of these contact sites rely on various regulators acting in diverse cellular processes. This review focuses on proteins at ER-PM contact sites, highlighting recent progress in their mechanisms and physiological roles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Antonio de Jesus Lopez-Fuentes, Karime Naid Nachon-Garduno, Fernando Suaste-Olmos, Ariadna Mendieta-Romero, Leonardo Peraza-Reyes
Summary: This study demonstrates the involvement of the reticulon protein RTN1 in shaping the endoplasmic reticulum and aiding in the process of ascospore formation and nuclear segregation during sexual development in the model fungus Podospora anserina. The proper functioning and positioning of the spindle during meiosis rely on accurate dynamics of the ER membrane, with proteins such as RTN1 playing a key role in regulating nuclear dynamics.
Review
Plant Sciences
Jiahui Bian, Xiao Su, Xiaoyan Yuan, Yuan Zhang, Jinxing Lin, Xiaojuan Li
Summary: This review focuses on the structural organization, dynamics, and physiological functions of membrane contact sites between the endoplasmic reticulum and various organelles, particularly in plants. Membrane contact sites play a vital role in development and response to stress by facilitating highly dynamic and organized interactions among organelles. The endoplasmic reticulum acts as an architectural scaffold to maintain the spatial distribution of other membrane-bound organelles. Future research directions related to membrane contact sites are also discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biology
Tricia T. Nguyen, Gia K. Voeltz
Summary: This study investigates how ER-associated mitochondrial nodes regulate the assembly of fission and fusion machinery on mitochondrial membranes. The researchers identified an ER membrane protein, ABHD16A, as a major regulator of node formation. The absence of ABHD16A results in the failure of fission and fusion machineries to recruit to the nodes, leading to decreased rates of fission and fusion.
Review
Cell Biology
Hannah T. Perkins, Viki Allan
Summary: The endoplasmic reticulum (ER) is a crucial organelle responsible for vital cellular processes, with its complex morphology and dynamics linked to its function and implications in various diseases. Bacteria and viruses can exploit the ER for their replication. Research focuses on understanding ER morphology, subdomains, membrane contact sites, quantifying ER motion, and exploring diseases associated with ER morphology and dynamics.
Review
Biochemistry & Molecular Biology
Vaishali Kumar, Shuvadeep Maity
Summary: Recent studies have highlighted the significance of inter organelle connections in maintaining cellular homeostasis, particularly the specialized physical connection between the endoplasmic reticulum (ER) and mitochondria at the mitochondria-associated ER membrane (MAM). While IRE1alpha and PERK are well-known for their canonical role as ER stress sensors, their potential non-canonical functions at the MAM beyond ER stress signaling have become a focus of current research.
Article
Multidisciplinary Sciences
Gunes Parlakgul, Ana Paula Arruda, Song Pang, Erika Cagampan, Nina Min, Ekin Guney, Grace Yankun Lee, Karen Inouye, Harald F. Hess, C. Shan Xu, Gokhan S. Hotamisligil
Summary: This study reveals the complex structural organization of organelles in liver tissue and highlights the significant alterations in obese mice. The functional importance of these structural changes on cellular and systemic metabolism is also demonstrated.
Article
Biochemistry & Molecular Biology
Menno Spits, Iris T. Heesterbeek, Lennard M. Voortman, Jimmy J. Akkermans, Ruud H. Wijdeven, Birol Cabukusta, Jacques Neefjes
Summary: The co-migration of ER with other organelles, facilitated by late endosomes and lysosomes, is a major mechanism controlling ER network architecture. This process alters local ER structure and links late endosomal/lysosomal positioning and mobility to ER network architecture.
Review
Biochemistry & Molecular Biology
Michela Rossini, Paola Pizzo, Riccardo Filadi
Summary: The strategic importance of inter-organelle cross-talk in regulating physiological cellular mechanisms is well established. Efforts have been made to study these organelle interactions and understand their functional roles within the cell. The available methods for investigating membrane contact sites have both advantages and disadvantages.
Article
Multidisciplinary Sciences
Sascha Waidmann, Chloe Beziat, Jonathan Ferreira Da Silva Santos, Elena Feraru, Mugurel I. Feraru, Lin Sun, Seinab Noura, Yohann Boutte, Juergen Kleine-Vehn
Summary: This study reveals a mechanism that integrates multiple external cues into auxin-dependent growth programs in Arabidopsis thaliana. This mechanism involves the regulation of PIN-LIKES (PILS) protein abundance and turnover, which affects nuclear auxin signaling and growth rates, contributing to stress-induced growth adaptation in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Clinical Neurology
Marijn Kuijpers, Phuong T. Nguyen, Volker Haucke
Summary: The neuronal endoplasmic reticulum (ER) is a dynamic, tubular network that extends throughout the neuron and plays crucial roles in calcium regulation, membrane dynamics, and the supply of ions and lipids to other organelles. Recent advances have highlighted the importance of the neuronal ER in axonal growth, repair, and synaptic function. Contact sites between the ER and other axonal organelles influence neurodevelopment and neurotransmission.
Review
Cell Biology
Sara Benhammouda, Anjali Vishwakarma, Priya Gatti, Marc Germain
Summary: Organelles in cells cooperate through membrane contact sites to regulate vital cellular functions, with Mitochondria-Endoplasmic-Reticulum (ER) contact sites (MERCS) playing a crucial role in controlling biological processes by regulating calcium and metabolic homeostasis. Various techniques, including fluorescence-based imaging, have been established to study these contact sites and identify molecular interactions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Klaas Yperman, Marijn Kuijpers
Summary: The endoplasmic reticulum (ER) is a crucial organelle within eukaryotic cells, with the ability to regulate calcium fluxes and communicate with other organelles. Its significance in neuronal processes like neurite growth, axon repair, and neurotransmission has gained considerable attention. This review highlights the importance of the ER tubular network in maintaining axonal homeostasis and emphasizes the cooperative efforts of ER-shaping proteins, cytoskeleton, and autophagy processes.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Maria Casas, Rut Fado, Jose Luis Dominguez, Aina Roig, Moena Kaku, Shigeru Chohnan, Montse Sole, Mercedes Unzeta, Alfredo Jesus Minano-Molina, Jose Rodriguez-Alvarez, Eamonn James Dickson, Nuria Casals
JOURNAL OF CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Maria Paz Prada, Arsalan U. Syed, Gopireddy R. Reddy, Miguel Martin-Aragon Baudel, Victor A. Flores-Tamez, Kent C. Sasse, Sean M. Ward, Padmini Sirish, Nipavan Chiamvimonvat, Peter Bartels, Eamonn J. Dickson, Johannes W. Hell, John D. Scott, Luis F. Santana, Yang K. Xiang, Manuel F. Navedo, Madeline Nieves-Cintron
NATURE COMMUNICATIONS
(2020)
Article
Physiology
Christopher Fortenbach, Gabriel Peinado Allina, Camilla M. Shores, Sarah J. Karlen, Eric B. Miller, Hannah Bishop, James S. Trimmer, Marie E. Burns, Edward N. Pugh
Summary: The study reveals that K(v)2.1 channels carry 70-80% of the non-NKX outward dark current in mouse rods, and their loss results in increased Ca2+ influx and intracellular Ca2+ levels, leading to progressive degeneration.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Silvia G. del Villar, Taylor L. Voelker, Maartje Westhoff, Gopireddy R. Reddy, Heather C. Spooner, Manuel F. Navedo, Eamonn J. Dickson, Rose E. Dixon
Summary: The number and activity of Ca(v)1.2 channels in cardiomyocytes regulates the magnitude of Ca2+-induced Ca2+ release and myocardial contraction. Stimulation of beta-Adrenergic receptors leads to insertion of Ca(V)1.2 channels into the sarcolemma, enhancing cooperative gating behavior and increasing Ca2+ influx and contractility. This process is fueled by an internal reserve of pre-synthesized channels localized in endosomes, regulated by Rab proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Candice Kutchukian, Oscar Vivas, Maria Casas, Julia G. Jones, Scott A. Tiscione, Sergi Simo, Daniel S. Ory, Rose E. Dixon, Eamonn J. Dickson
Summary: In this study, it was found that dysfunction of the lysosomal Niemann-Pick C1 (NPC1) cholesterol transporter initiates a signaling cascade that alters the cholesterol/phosphatidylinositol 4-phosphate (PtdIns4P) countertransport cycle between the Golgi-endoplasmic reticulum and lysosome-endoplasmic reticulum membrane contact sites. These disruptions in signaling pathways lead to abnormal intracellular trafficking and growth signaling.
Review
Biochemistry & Molecular Biology
Nicholas C. Vierra, James S. Trimmer
Summary: Ion channels in excitable cells play a concerted function in shaping signaling events by controlling membrane electrical properties. Specific groups of ion channels have been recognized to be functionally coupled in mediating ionic fluxes that impact membrane potential, and changes in membrane potential also impact ion channel gating. Recent studies have identified distinct sets of ion channels that can physically and functionally associate to regulate the function of one another. This review discusses canonical and non-canonical ion channel partnerships and their impact.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nicholas C. Vierra, Samantha C. O'Dwyer, Collin Matsumoto, L. Fernando Santana, James S. Trimmer
Summary: In mammalian brain neurons, membrane depolarization triggers Ca2+ influx through voltage-gated Ca2+ channels, leading to diverse cellular responses, including gene expression. The L-type Ca2+ channels in neuronal soma and dendrites play a key role in this process. The organization of signaling complexes by Kv2.1 at endoplasmic reticulum-plasma membrane junctions enhances the clustering and activity of Cav1.2 channels, impacting somatic Ca2+ signals and excitation-transcription coupling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Scott A. Tiscione, Maria Casas, Jonathan D. Horvath, Vincent Lam, Keiko Hino, Daniel S. Ory, L. Fernando Santana, Sergi Simo, Rose E. Dixon, Eamonn J. Dickson
Summary: The study shows that loss-of-function, knockout, or neurodegenerative disease-causing mutations in NPC1 can lead to damaging alterations in the expression and distribution of IP3R1, causing cell death. This process is mediated by SREBP-dependent increases in PS1, with mutants of PS1 recapitulating the Ca2+ phenotypes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Eamonn J. Dickson
Summary: Phosphoinositides, a family of signaling lipids, play a crucial role in regulating protein function at the interface between cellular membranes. Mutations or changes in phosphoinositide metabolizing enzymes can lead to various disorders, such as developmental, neurodegenerative, and metabolic disorders, which have devastating effects on human health. This review focuses on the transport and metabolism of phosphoinositide lipids at membrane contact sites.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Review
Physiology
Jill B. Jensen, Bjoern H. Falkenburger, Eamonn J. Dickson, Lizbeth de la Cruz, Gucan Dai, Jongyun Myeong, Seung-Ryoung Jung, Martin Kruse, Oscar Vivas, Byung-Chang Suh, Bertil Hille
Summary: Phosphoinositide membrane lipids, as low-abundance signaling molecules, play important roles in cellular processes and are regulated by physiological stimuli. Recent biophysical experiments have measured and modeled the dynamics and regulation of these signals, revealing their control over rapid physiological responses.
JOURNAL OF GENERAL PHYSIOLOGY
(2022)
Review
Neurosciences
Henry M. Colecraft, James S. Trimmer
Summary: Selective ion channel modulators are important in studying physiological function and developing therapeutic strategies. Renewable and recombinant antibodies can be used to control ion channel function, and this review discusses their different forms and mechanisms. Intrabodies, a type of recombinant antibody expressed intracellularly, are highlighted as a genetic tool to control ion channel function. The review also suggests potential future research directions for engineering recombinant antibodies for enhanced utility in ion channel research.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Multidisciplinary Sciences
Maria Casas, Karl D. Murray, Keiko Hino, Nicholas C. Vierra, Sergi Simo, James S. Trimmer, Rose E. Dixon, Eamonn J. Dickson
Summary: The loss of NPC1 function alters the nanoscale distribution and function of ion channels, leading to abnormal calcium entry, mitochondrial dysfunction, and neurotoxicity. These findings provide insights into the molecular mechanisms connecting NPC1 loss to NPC-associated neuropathology.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Biophysics
Taylor L. Voelker, Silvia Garcia del Villar, Maartje F. Westhoff, Andrea M. Coleman, Mary C. Horne, Johannes Hell, Eamonn J. Dickson, Rose E. Dixon
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Maartje Westhoff, Silvia G. del Villar, Taylor L. Voelker, Eamonn J. Dickson, Rose E. Dixon
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Silvia Garcia del Villar, Eamonn J. Dickson, Rose E. Dixon
BIOPHYSICAL JOURNAL
(2020)
