Review
Engineering, Biomedical
Dandan Zheng, Huitong Ruan, Wei Chen, Yuhui Zhang, Wenguo Cui, Hao Chen, Hongxing Shen
Summary: Extracellular vesicles (EVs) are nano-scale vesicles derived by cell secretion with unique advantages, which benefit their wide applications in regenerative medicine. However, the in vivo therapeutic effect of EVs still greatly restricted by several obstacles. This review summarizes different strategies to enhance EV functions and introduces the latest developments of functionalized EV-loaded biomaterials in different diseases. Challenges and future directions of EVs are also discussed.
BIOACTIVE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Shenghai Shen, Langrun Deng, Yiheng Du, Jingjing Gao, Chuanhao Zhang, Yueduo Wang, Zhewen Shen, Yixuan Li, Xiaowen Chen, Hao Chen
Summary: This study provides an in-depth qualitative and quantitative overview of research on biological wound dressings in recent decades. The results show significant progress and extensive global collaboration in the field, with clear knowledge base. The hotspots of research include chitosan hydrogels, bacterial cellulose, active agents, and electrospinning fibers, while the frontiers of research include novel starting materials, precise release systems, and clinical and regenerative medicine applications.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Chemistry, Multidisciplinary
Yuvaraj Arun, Radhakanta Ghosh, Abraham J. Domb
Summary: Biodegradable, hydrophobic, and injectable liquid polymers are able to achieve sustained, local drug release, with potential applications in regenerative medicine.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Anna L. Kersey, Thuy-Uyen Nguyen, Biswadeep Nayak, Irtisha Singh, Akhilesh K. Gaharwar
Summary: Recent developments in biomedical engineering have focused on designing smart and bio-responsive materials. Recent technological advances in biology, specifically in omics techniques, can provide high-throughput functional readouts of the cellular and molecular interactions. These omics-based approaches have the potential to revolutionize biomedical research and replace traditional trial and error approaches in designing the next-generation of biomaterials.
Review
Engineering, Biomedical
Vishnu Priya Murali, Christina A. Holmes
Summary: This review summarizes various biomaterial-based extracellular vesicle (EV) delivery systems used in preclinical tissue regeneration studies and other therapeutic applications. EV-based therapies have shown success in preclinical models for biomedical applications, but the challenge lies in prolonging EV retention at target sites for improved therapeutic efficacy through biomaterial-based systems.
ACTA BIOMATERIALIA
(2021)
Review
Biochemistry & Molecular Biology
Teruki Nii
Summary: Gelatin particles, serving as drug carriers, exhibit excellent drug release properties and are effective in wound healing and drug screening models.
Article
Biotechnology & Applied Microbiology
Limei Wang, Kedi Xia, Lu Han, Min Zhang, Jihuan Fan, Liu Song, Anqi Liao, Wenyu Wang, Jianfeng Guo
Summary: The study investigated a new therapeutic strategy using Ginkgolide B (GB) and high molecular weight hyaluronan (HA) hydrogel to treat chronic wounds in diabetic patients, showing significant effects on anti-inflammation, promoting healing, and inducing angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Yasmeen Shamiya, Shruthi Polla Ravi, Ali Coyle, Subrata Chakrabarti, Arghya Paul
Summary: This article reviews emerging therapeutic nanoparticles for treating diabetic wounds and discusses their discrete delivery mechanisms and sites of action. The current challenges of these nanoengineered materials for successful clinical translation are critically assessed, along with their potential for growth in the clinical marketplace.
DRUG DISCOVERY TODAY
(2022)
Review
Materials Science, Multidisciplinary
Tao Yang, Yingfan Chen, Yajing Xu, Xiangyu Liu, Mingying Yang, Chuanbin Mao
Summary: Viruses as biomaterials exhibit minimal toxicity, good biocompatibility, and well-defined structures. They have been utilized in various applications such as targeted therapy, antimicrobial agents, tissue regeneration, disease detection, bioimaging, vaccines, and immunotherapy. This review discusses the exciting applications and the challenges and future directions of using virus-based biomaterials.
MATERIALS SCIENCE & ENGINEERING R-REPORTS
(2023)
Article
Pharmacology & Pharmacy
Liu Song, Hao Yang, Di Liang, Di Chu, Leilei Yang, Meng Li, Bo Yang, Ying Shi, Zheng Chen, Zhuo Yu, Jianfeng Guo
Summary: Chlorogenic acid shows potential in promoting diabetic wound healing by reducing inflammation, enhancing re-epithelialization and angiogenesis, and facilitating collagen remodeling.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Review
Engineering, Biomedical
Jinchun Ye, Chang Xie, Canlong Wang, Jiayun Huang, Zi Yin, Boon Chin Heng, Xiao Chen, Weiliang Shen
Summary: Studies have shown that active modulation of immune cells, particularly macrophages, via biomaterials is an effective way to control immune response, promote tissue regeneration, and facilitate biomaterial integration.
BIOACTIVE MATERIALS
(2021)
Review
Polymer Science
Anastasia A. Sherstneva, Tatiana S. Demina, Ana P. F. Monteiro, Tatiana A. Akopova, Christian Grandfils, Ange B. Ilangala
Summary: This critical review surveys the most relevant materials and processing techniques for preparing biodegradable microparticles, with a focus on their potential applications in clinical practices, considering regulatory constraints and the feasibility of industrial production.
Article
Chemistry, Multidisciplinary
Aazam Shaikh, Prashant Kesharwani, Virendra Gajbhiye
Summary: Dendrimers have a wide range of applications in biomedical fields, including cargo delivery, nucleic acid delivery, and macromolecule delivery. They can also be used to crosslink synthetic and natural polymeric scaffolds and mimic the extracellular matrix. Dendrimers play a crucial role in regulating cell behavior and delivering bioactive molecules, and have shown significant results in stem cell survival, osteogenesis, and repair.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Engineering, Biomedical
Hung-Pang Lee, Kathy Xiao Cai, Ting-Ching Wang, Ryan Davis Jr, Kaivalya Deo, Kanwar Abhay Singh, Tanmay P. Lele, Akhilesh K. Gaharwar
Summary: Granular hydrogels assembled through the jamming process have great potential for biomedical applications such as tissue regeneration, drug/cell delivery, and 3D printing. However, current methods for interconnecting the microgels often have limitations, necessitating postprocessing for crosslinking. To address this, we introduced a thiol-functionalized thermo-responsive polymer into oxidized hyaluronic acid microgel assemblies. This two-stage crosslinking system provides injectability, shape stability, mechanical integrity, and sustained drug release, making it suitable for various biomedical applications.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Review
Pharmacology & Pharmacy
Sergio Ayala-Mar, Belen Rodriguez-Morales, Pedro Chacon-Ponce, Jose Gonzalez-Valdez
Summary: Extracellular vesicles, particularly exosomes, play significant roles in cardiometabolic pathophysiology by serving as communication mediators, diagnostic markers, drug delivery vehicles, and tools for gene therapy and regenerative medicine. Their incorporation into innovative biomaterials enhances tissue regeneration and engineering.
Article
Biochemistry & Molecular Biology
Matthias Ostermeier, Steffen Heinz, Julia Hamm, Jure Zabret, Anna Rast, Andreas Klingl, Marc M. Nowaczyk, Joerg Nickelsen
Summary: Thylakoids are specialized internal membrane systems that house photosynthetic electron transport machinery in cyanobacteria and chloroplasts. The proteins AncM and CurT have antagonistic roles in shaping thylakoid ultrastructure.
Article
Plant Sciences
Magdalena Fuessl, Ann-Christine Koenig, Jurgen Eirich, Markus Hartl, Laura Kleinknecht, Alexandra-Viola Bohne, Anne Harzen, Katharina Kramer, Dario Leister, Joerg Nickelsen, Iris Finkemeier
Summary: This study identified dynamic changes in protein lysine acetylation in Chlamydomonas under different growth conditions, particularly in enzymes related to photosynthesis, fatty acid metabolism, and the glyoxylate cycle depending on acetate and light. The newly discovered lysine acetylation sites may be valuable for genetic engineering of metabolic pathways in Chlamydomonas.
Review
Engineering, Biomedical
Javiera Sanhueza Ortega, Rocio Corrales-Orovio, Peter Ralph, Jose Tomas Egana, Carmine Gentile
Summary: 3D bioprinting technology has the potential to revolutionize the biomedical field by enabling tissue engineering and regeneration. However, there are challenges to be addressed, such as the lack of tissue oxygenation in bioprinted tissues and organs. Recent approaches, including the use of photosynthetic microorganisms, offer promising solutions to improve tissue oxygenation. This review summarizes the state-of-the-art approaches and discusses the application of photosynthetic microorganisms in 3D bioprinting.
ACTA BIOMATERIALIA
(2023)
Article
Critical Care Medicine
Fabian Weiss, Kariem Agua, Andrea Weinzierl, Anna Schuldt, Jose Tomas Egana, Anna Melissa Schlitter, Katja Steiger, Hans-Guenther Machens, Yves Harder, Daniel Schmauss
Summary: Burn wound progression and intercalated unburned interspaces were evaluated using a burn comb model and a fixed dorsal frame. The frame's durability was tested and compared between a 10-second burn and a 60-second burn. The study showed that the burn comb model and fixed frame allowed for assessment of wound progression and potential treatments for burns.
JOURNAL OF BURN CARE & RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
L. B. Leibrock, D. M. Hofmann, B. Fuchs, A. Birt, M. Reinholz, A. Guertler, K. Frank, R. E. Giunta, J. T. Egana, J. Nickelsen, T. L. Schenck, N. Moellhoff
Summary: This study developed a method to isolate total RNA from bioactivated scaffolds and proposed a new approach to determine living bacteria using real-time PCR. The gene expression patterns differed in vitro and in vivo, with 16S and rnpA showing varying expression levels in scaffold environments compared to liquid cultures.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Plant Sciences
Daniel Neusius, Laura Kleinknecht, Jing Tsong Teh, Matthias Ostermeier, Simon Kelterborn, Jurgen Eirich, Peter Hegemann, Iris Finkemeier, Alexandra-Viola Bohne, Joerg Nickelsen
Summary: The dihydrolipoamide acetyltransferase subunit DLA2 of the chloroplast pyruvate dehydrogenase complex (cpPDC) in the green alga Chlamydomonas reinhardtii plays important roles in carbon metabolism and D1 synthesis. By forming a ribonucleoprotein particle with psbA mRNA, DLA2 is involved in the synthesis of D1 protein in the photosystem II reaction center. Acetylation of DLA2 induces its release from cpPDC and promotes its functional shift towards RNA binding.
Article
Engineering, Biomedical
Rocio Corrales-Orovio, Felipe Carvajal, Christopher Holmes, Miguel Miranda, Sergio Gonzalez-Itier, Camila Cardenas, Constanza Vera, Thilo L. Schenck, Jose Tomas Egana
Summary: A photosynthetic biomaterial that can release oxygen and other bioactive molecules to promote wound healing was developed in this study. The material demonstrated high biocompatibility and oxygen release capacity in vitro and in vivo. It was successfully applied to the skin surface of 20 healthy volunteers without any skin irritation. Moreover, the biomaterial showed sustained release of important bioactive molecules. Overall, photosynthetic biomaterials have great potential as an effective approach for wound healing.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Christopher Holmes, Juan Varas, Sebastian San Martin, Jose Tomas Egana
Summary: This study presents a 3D photosynthetic tumor model composed of human melanoma cells and Chlamydomonas reinhardtii microalgae, which allows for simultaneous study of both cell types. The results show that the two cell types are biocompatible with each other under cell culture conditions, and a 10:1 ratio of microalgae to cells meets the metabolic requirement of the tumor cells, producing sufficient oxygen. This 3D tumor model provides an easy-to-use in vitro resource for analyzing the effects of photosynthetically produced oxygen on a tumor microenvironment, opening up various potential research avenues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Fei Wang, Korbinian Dischinger, Lisa Desiree Westrich, Irene Meindl, Felix Egidi, Raphael Troesch, Frederik Sommer, Xenie Johnson, Michael Schroda, Joerg Nickelsen, Felix Willmund, Olivier Vallon, Alexandra-Viola Bohne
Summary: In plants and cyanobacteria, the binding of chlorophyll to the D1 polypeptide of photosystem II (PSII) requires a complex involving HCF244/Ycf39 and OHP1/OHP2 proteins. In a Chlamydomonas mutant lacking the OHP2 protein, core PSII subunits, especially D1, fail to accumulate. However, extragenic suppressors suggest alternative pathways for chlorophyll association to PSII. The data indicate that OHP2 is not required for psbA translation in Chlamydomonas, but is necessary for stabilizing D1.
Article
Multidisciplinary Sciences
Camila Cardenas-Calderon, Valentina Veloso-Gimenez, Tamara Gonzalez, Aniela Wozniak, Patricia Garcia, Sebastian San Martin, Juan F. Varas, Ivo Carrasco-Wong, Mario Vera, Jose Tomas Egana
Summary: This study aims to study the functional biofilms in physiological conditions and their potential effects on health and regeneration. The results show that biofilm infection induces leucocyte infiltration, elevated interferon-gamma and interleukin-10, as well as high systemic pro-calcitonin concentrations, indicating significant effects of biofilm infection on the host.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Miguel L. Obaid, Felipe Carvajal, Juan Pablo Camacho, Rocio Corrales-Orovio, Ximena Martorell, Juan Varas, Wilfredo Calderon, Christian Dani Guzman, Marianne Brenet, Margarita Castro, Cecilia Orlandi, Sebastian San Martin, Antonio Eblen-Zajjur, Jose Tomas Egana
Summary: Proper oxygen supply is crucial for the regeneration process, but vascular disruption in wounds reduces oxygen availability. The development of novel biomaterials and improved clinical procedures to promote wound oxygenation is an active research field. This case report demonstrates the long-term safety and applicability of photosynthetic scaffolds for dermal regeneration and their compatibility with other surgical procedures. Furthermore, dermal vacuum massage therapy can further improve clinical outcomes and enhance the patient's quality of life, supporting the translation of photosynthetic therapies into human patients.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Carolina Ehrenfeld, Valentina Veloso-Gimenez, Rocio Corrales-Orovio, Rolando Rebolledo, Mauricio P. Boric, Jose Tomas Egana
Summary: As animal cells cannot produce oxygen, erythrocytes are responsible for gas interchange, being able to capture and deliver oxygen upon tissue request. Several other cells in nature produce oxygen by photosynthesis, raising the question of whether they could circulate within the vascular networks, acting as an alternative source for oxygen delivery. This study examines the physical and mechanical features of the photosynthetic microalga Chlamydomona reinhardtii and compares them with erythrocytes, finding similarities in size and rheological properties. Furthermore, the biocompatibility of the microalgae is evaluated, showing that they can co-culture with endothelial cells without affecting each other's morphology and viability. Additionally, the systemic injection of high numbers of microalgae in mice does not trigger harmful responses, suggesting their potential as a source of oxygen delivery.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ian Small, Joanna Melonek, Alexandra-Viola Bohne, Joerg Nickelsen, Christian Schmitz-Linneweber
Summary: Plant organellar RNA metabolism is regulated by numerous nucleus-encoded RNA-binding proteins (RBPs) that control RNA stability, processing, and degradation. These post-transcriptional processes are critical for the production of essential components in chloroplasts and mitochondria, which are necessary for organellar biogenesis and plant survival. While many organellar RBPs have been identified, our understanding of their mechanisms and kinetics in fulfilling their functions remains incomplete. This review provides a comprehensive summary of plant organellar RNA metabolism, focusing on the mechanistic aspects and kinetics of RBP functions.
Article
Cell Biology
Valentina Castillo, Pamela Diaz-Astudillo, Rocio Corrales-Orovio, Sebastian San Martin, Jose Tomas Egana
Summary: Throughout an organism's lifespan, tissue regeneration capabilities vary significantly. We conducted a comparative analysis of skin derived from mice at regenerative and reparative stages, and found differences in molecular composition, structure, and functionality between the two types of skin. This study provides valuable insights for developing new biomaterials and strategies for tissue engineering and regeneration.
Article
Biochemical Research Methods
Suzan Ozugur, Myra N. Chavez, Rosario Sanchez-Gonzalez, Lars Kunz, Joerg Nickelsen, Hans Straka
Summary: This technology involves injecting phototrophic microalgae into the brain, triggering photosynthetic oxygen production to rescue neuronal activity under hypoxic conditions, potentially ameliorating consequences of hypoxia under pathological conditions.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
