期刊
BIOMATERIALS
卷 104, 期 -, 页码 43-51出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.07.009
关键词
Antimicrobial peptides; Gold-nanoparticle; A3-APO; S.Typhimurium
资金
- MEST [NRF-2014R1A2A1A11050606, NRF-2011-0021810, 20110017532]
- Global Research Laboratory from the National Research Foundation of Korea [NRF-2014K1A1A2064460]
- Next-Generation BioGreen 21 Program (SSAC), Rural Development Administration, Republic of Korea [PJ01117703]
- Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea [914010-04-1-HD020]
Antimicrobial peptides (AMPS) are a promising new class of antibacterial compounds. However, their applications in the treatment of intracellular pathogenic bacteria have been limited by their in vivo instability and low penetrating ability into mammalian cells. Here, we report that gold nanoparticles conjugated with DNA aptamer (AuNP-Apt) efficiently delivered AMPs into mammalian living systems with enhanced stability of the AMPs. C-terminally hexahistidine-tagged A3-APO (A3-APO(His)) AMPs were loaded onto AuNPs conjugated with His-tag DNA aptamer (AuNP-Apt(His)) by simple mixing and were delivered into Salmonella enterica serovar Typhimurium (S. Typhimurium)-infected HeLa cells, resulting in the increased viability of host cells due to the elimination of intracellular S. Typhimurium cells. Furthermore, the intravenous injection of AuNP-Apt(His) loaded with A3-APO(His) into S. Typhimurium-infected mice resulted in a complete inhibition of S. Typhimurium colonization in the mice organs, leading to 100% survival of the mice. Therefore, AuNP-AptH's can serve as an innovative platform for AMP therapeutics to treat intracellular bacterial infections in mammals. (C) 2016 Elsevier Ltd. All rights reserved.
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