Article
Medicine, General & Internal
Wendong Ju, Simao Fu, Jun Huang, Bishuang Li, Li Wang, Minmin Zheng, Haojie Song, Quanyong Li, Qiaoyun Zhou, Meixing He
Summary: The combination of UCMSCs with a PRF membrane showed positive clinical effects in treating lower limb ischemia in an elderly patient. Further studies are needed to confirm the efficacy and safety of this treatment approach.
Article
Cell & Tissue Engineering
Chi Zhang, Yinuo Lin, Ke Zhang, Luyang Meng, Xinyang Hu, Jinghai Chen, Wei Zhu, Hong Yu
Summary: The study found that GDF11 can enhance the therapeutic effects of stem cells for ischemic diseases by increasing stem cell viability, mobility, and angiogenic paracrine functions.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Jia Wang, Yue Sun, Yiping Liu, Jize Yu, Xiaolin Sun, Lin Wang, Yanmin Zhou
Summary: This study investigated the existence of MSCs in Schneiderian membrane and the effects of PRF on these cells. The results showed that MSCs existed in Schneiderian membrane and PRF stimulation promoted cell proliferation, migration, and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation could accelerate bone healing and increase the quantity and quality of new bone.
CELL COMMUNICATION AND SIGNALING
(2022)
Review
Biochemistry & Molecular Biology
Santino Blando, Ivan Anchesi, Emanuela Mazzon, Agnese Gugliandolo
Summary: Spinal cord injury (SCI) is a debilitating disease that results in the loss of motor and sensory neurons. Tissue engineering techniques using stem cells, especially mesenchymal stem cells (MSCs), have shown promise in promoting neuronal regeneration. Current methods of cell insertion have low rates of success, so researchers are focusing on using bioresorbable scaffolds to improve cell survival and engraftment. Various materials are being studied as scaffolds to decrease inflammation and increase cell engraftment and survival. Clinical trials and animal models have demonstrated positive results with the use of MSC-enriched biomaterial scaffolds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Abhishek Sahu, Jin Jeon, Min Suk Lee, Hee Seok Yang, Giyoong Tae
Summary: Mesenchymal stem cell (MSC) based therapy shows great potential in treating diseases, but the low viability of transplanted cells in injured tissues due to high oxidative stress levels is a major obstacle. Using a ROS-scavenging nanozyme to protect MSCs from oxidative stress-mediated apoptosis can significantly improve therapeutic potential and survival in high oxidative stress conditions. Incorporating nanozymes into MSCs is a simple and efficient way to enhance MSC-based cell therapy.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Cell Biology
Farina Mohamad Yusoff, Yukihito Higashi
Summary: Mesenchymal stem/stromal cells (MSCs) have medicinal properties for vascular regeneration. Various conditions, like chronic arterial occlusive disease and diabetic ulcers, cause significant morbidity and MSCs offer treatment options through therapeutic angiogenesis. This review discusses the use of MSCs for therapeutic angiogenesis in vascular research and medicine, either as a monotherapy or in combination with conventional interventions, for treating patients with peripheral artery diseases.
Article
Cell Biology
Kenon Chua, Fui Ping Lim, Victor Kwan Min Lee, Toan Thang Phan, Bee Choo Tai, Yih Kai Tan
Summary: In this study, human Cord Lining Mesenchymal Stem Cells (CL-MSCs) were used in a rabbit hindlimb ischemia model to stimulate neovascularization. The results showed that CL-MSCs had a positive effect on angiogenesis, with more CD31 positive cells and capillaries found in the treated muscles. Additionally, there was a non-statistically significant increase in blood perfusion in the treatment group, and CL-MSCs showed no toxicity or significant local immune reaction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Arturo Ibanez-Fonseca, Ana Rico, Silvia Preciado, Fernando Gonzalez-Perez, Sandra Muntion, Jesus Garcia-Brinon, Maria-Carmen Garcia-Macias, Jose Carlos Rodriguez-Cabello, Miguel Pericacho, Matilde Alonso, Fermin Sanchez-Guijo
Summary: Combination of an elastin-like recombinamer (ELR)-based hydrogel with mesenchymal stromal cells (MSCs) shows potential in improving tissue reperfusion and blood vessel density in a murine hindlimb ischemia model, suggesting a promising approach for clinical application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Adel Hinsenkamp, Kiara Kun, Fatime Gajnut, Aliz Majer, Zsombor Lacza, Istvan Hornyak
Summary: Fibrin membranes are widely used in regenerative medicine due to their biocompatibility, biodegradability, high growth factor content, and cell attachment support. Cryoprecipitate isolation can increase fibrinogen and platelet concentration in plasma, affecting membrane thickness and growth factor content. However, membrane thickness does not significantly influence cell attachment, as demonstrated by live-dead staining and XTT measurements.
Article
Cell & Tissue Engineering
Pegah Nammian, Seyedeh-Leili Asadi-Yousefabad, Sajad Daneshi, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Vahid Razban
Summary: The study compared the effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in the treatment of critical limb ischemia (CLI). The results indicated that BM-MSCs had a stronger effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared to AT-MSCs. This suggests that BM-MSC transplantation could be a more effective therapy for diseases with insufficient angiogenesis, such as hindlimb ischemia.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Immunology
Jiale Li, Qinbao Peng, Ronghua Yang, Kunsheng Li, Peng Zhu, Yufeng Zhu, Pengyu Zhou, Gabor Szabo, Shaoyi Zheng
Summary: This review discusses the use of machine perfusion and mesenchymal stem cells to improve the quality and outcomes of marginal organs in transplantation. By continuously perfusing the organs to mimic physiological conditions, evaluating pre-transplant organ function, and treating the organs with pharmacological, gene, and stem cell therapy, the quality and adaptability of the organs can be enhanced. Promising preclinical results indicate the potential clinical translation of this innovative strategy to improve the quality of marginal grafts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hui Hu, Xiaowei Hu, Lin Li, Yan Fang, Yan Yang, Jingjing Gu, Jiadong Xu, Lisheng Chu
Summary: Exosomes derived from bone mesenchymal stem cells (BMSC-Exos) can promote angiogenesis and improve neurological functions in ischemic stroke mice via upregulation of miR-21-5p. In vitro experiments show that BMSC-Exos also enhance the functions of human umbilical vein endothelial cells (HUVECs).
Article
Engineering, Biomedical
Maximilian G. Burger, Andrea Grosso, Priscilla S. Briquez, Gordian M. E. Born, Alexander Lunger, Flavio Schrenk, Atanas Todorov, Veronica Sacchi, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: In regenerative medicine, rapid vascularization of clinical-size bone grafts is a challenge that has not been solved. This study investigates the distribution of VEGF protein on matrices to achieve efficient coupling of angiogenesis and bone formation, providing a clinically applicable strategy for engineering vascularized bone.
ACTA BIOMATERIALIA
(2022)
Article
Pharmacology & Pharmacy
Tao Chen, Bo Ye, Jing Tan, Haifeng Yang, Faming He, Raouf A. Khalil
Summary: Mesenchymal stem cells, especially those expressing CD146, show potential in improving vascularity and muscle function in a rat model of hind-limb ischemia, reducing apoptosis and increasing vascular endothelial growth factor expression.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Cell & Tissue Engineering
Yen-Ta Chen, Fei-Chi Chuang, Chih-Chao Yang, John Y. Chiang, Pei-Hsun Sung, Yi-Ching Chu, Chi-Ruei Huang, Kuan-Hui Huang, Hon-Kan Yip
Summary: The combined use of melatonin and adipose-derived mesenchymal stem cells effectively protects the testis against acute testicular torsion-induced ischemia-reperfusion injury.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.