期刊
FOOD CHEMISTRY
卷 310, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125854
关键词
Lycium barbarum L.; Carotenoids; Zeaxanthin dipalmitate; HPLC analysis; Ultrasonic-assisted extraction; High-speed counter-current chromatography
资金
- Key Research and Development Program of Ningxia Hui Autonomous Region [2019BFG02026]
- Pilot project by Ningxia Academy of Agriculture and Forestry Sciences [YES16-0506, KJT2016001, DWHZC-201601, DWX-2018029]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Zeaxanthin dipalmitate (ZDP) is a major non-saponified carotenoid in fully ripe fruits of Lycium barbarum L. In the present study, response surface methodology was used to optimize the ultrasonic-assisted extraction (UAE) conditions of carotenoids from the fruits of L. barbarum, and the optimal extraction conditions were determined as follows: ultrasonic power of 360 W, ultrasonic time of 40 min and the ratio of extraction solvent to sample of 30 mL/g. An actual value of ZDP content of 5.40 mg/g and short extraction time indicated the efficiency of UAE. Furthermore, a promising high-speed counter-current chromatography (HSCCC) method was established for the purification of ZDP from the fruits of L. barbarum. With a developed two-phase solvent system composed of nhexane/dichloromethane/acetonitrile (10/3/7, v/v/v), ZDP with a purity of higher than 95% was successfully isolated from the crude extract. This is the first report on the purification of ZDP by using HSCCC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据