Article
Chemistry, Analytical
Xiaojin Zhang, Huimin Dou, Xiaorui Chen, Meihua Lin, Yu Dai, Fan Xia
Summary: Solid-state nanopores have a wide range of applications in various fields, such as DNA sequencing, energy conversion and storage, seawater desalination, sensors, and reactors. They offer high stability and controllable geometry, making them suitable for qualitative and quantitative analysis of different molecules. This review summarizes the past decade's research on nucleic acid amplification strategies for enhancing the sensitivity of solid-state nanopore sensors and discusses the future prospects and challenges in this field.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Y. U. Chun-Miao, W. A. N. G. Ye-Sheng, Z. H. U. Zhen-Tong, L. Bing-Ling
Summary: With the rapid development of nanopore sequencing technique, more attention is being paid to nanopore. DNA has expanded from a carrier of genetic information to a programmable functional material, creating a need for comprehensive characterization of DNA assembly. Traditional characterization methods often fail to provide sufficient structural information, necessitating the combination of various technologies. Solid-state nanopores show promise as an effective means of characterizing DNA nanostructures, such as analyzing finer nanostructures or distinguishing mixed nanostructures in complex samples.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Zhuoer Zeng, Rong Zhou, Ruowei Sun, Xun Zhang, Zeneng Cheng, Chuanpin Chen, Qubo Zhu
Summary: Hybridization chain reaction (HCR) can be categorized into linear and nonlinear types. Traditional linear HCR is limited by slow kinetics and low sensitivity, while nonlinear HCR, triggered by an initiator, can self-assemble into dendritic or branched DNA nanostructures with exponential growth kinetics, offering advantages such as rapid growth, high sensitivity, and ease of operation.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Yingying Chen, Xue Gong, Yuhui Gao, Yu Shang, Jinhua Shang, Shanshan Yu, Ruomeng Li, Shizhen He, Xiaoqing Liu, Fuan Wang
Summary: Introducing a novel endogenous DNA repairing enzyme-powered pre-selection strategy, a cancer cell-selective catalytic DNA circuit for imaging microRNA with high anti-interference capacity was developed. This circuit offers a reliable amplification strategy for intracellular biomarker assays in living systems.
Article
Chemistry, Analytical
Shiyi Wang, Ziwen Zong, Jianguo Xu, Bangben Yao, Zhou Xu, Li Yao, Wei Chen
Summary: In this study, a visual strategy for rapid and ultrasensitive detection of OTA was proposed by integrating primer-mediated exponential rolling circle amplification (P-ERCA) with a designed nucleic acid lateral flow strip (LFS). This method demonstrates highly sensitive detection of OTA and could be utilized as a universal method for detection of other chemical analytes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mengyan Li, Nian Luo, Xiaofei Liao, Li Zou
Summary: We developed a dual signal amplification strategy for sensitive detection of circulating tumor DNA (ctDNA) based on hybridization chain reaction (HCR) and proximity hybridization-regulated CRISPR/Cas12a. This strategy exhibits high sensitivity toward ctDNA with a low detection limit of 5.43 fM, and it has been successfully applied for ctDNA detection in serum samples, showing great potential in the clinical diagnosis of cancer.
Article
Nanoscience & Nanotechnology
Ruiping Wu, Yesheng Wang, Zhentong Zhu, Chunmiao Yu, Huan Li, Bingling Li, Shaojun Dong
Summary: Solid-state nanopores have shown potential for single-molecular characterization of nucleic acid assemblies and molecular machines. By introducing high-dielectric additives into the test buffer, noise reduction and resolution enhancement can be achieved to directly recognize small dimensional assemblies. This innovation allows for high specificity and improved resolution in distinguishing small molecules without the need for covalent labeling or additional signaling strategies.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Pei Dong, Ruomeng Li, Shizhen He, Qingqing Zhang, Jinhua Shang, Yuqian Jiang, Xiaoqing Liu, Fuan Wang
Summary: The accurate identification of multiple biomarkers involved in disease is crucial for distinguishing cancer cells from normal cells and facilitating reliable cancer diagnosis. In this study, a compact and clamped cascaded DNA circuit was engineered to specifically discriminate cancer cells from normal cells using the amplified multi-microRNA imaging strategy. The proposed DNA circuit combined the traditional cascaded DNA circuit with multiply localized responsive character, streamlining the circuit components and realizing localization-intensified cascaded signal amplification. The sequential activations of the compact circuit by multiple microRNAs, combined with a handy logic operation, significantly improved the cell-discriminating reliability.
Article
Chemistry, Analytical
Cheng Fang, Yuming Li, Song Hu, Hao Wang, Xiaoxia Chen, Xiaoli Zhu
Summary: The developed DNA tree in this study enables amplified analysis of mRNA in living cells, achieving sensitive, accurate, and nondestructive analysis. The probes are transported into cells by exosomes, are LDNAs resistant to degradation, laying the foundation for reliable analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Selma Piranej, Alisina Bazrafshan, Khalid Salaita
Summary: The authors introduce a new paradigm for DNA computation using dynamic DNA-based motors to transduce chemical inputs into mechanical outputs in the form of macroscopic locomotion. This method shows fast computation speeds and easy electronic detection.
NATURE NANOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Chenguang Wang, Yi Xu, Shuainan Li, Yi Zhou, Qiuling Qian, Yifan Liu, Xianqiang Mi
Summary: Circulating tumor cells (CTCs) are biomarkers widely used for the early detection, diagnosis, and therapy monitoring of cancer. However, due to their rarity in peripheral blood, isolating and analyzing CTCs with high efficiency, purity, and viability has been a significant technological challenge. In this study, the researchers developed a microfluidic system combining tetrahedral DNA framework, herringbone channel chip, and aptamer-triggered hybridization chain reaction for efficient capture and release of simulated CTCs.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Analytical
Shihua Luo, Ye Zhang, Guoni Huang, Bo Situ, Xinyi Ye, Maliang Tao, Yifang Huang, Bo Li, Xiujuan Jiang, Qian Wang, Lei Zheng
Summary: This study developed a novel electrochemical biosensor for sensitive detection of ctDNA, utilizing wheel-like catalytic hairpin assembly and frame hybridization chain reaction, which significantly enhances the sensitivity of ctDNA detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Rujian Zhao, Chunxu Yu, Baiyang Lu, Bingling Li
Summary: We have reported a universal and signal-on detection platform by coupling the CRISPR-Cas12a system with HCR. By changing the crRNA, different targets can be detected using the CRISPR-HCR pathway. The CRISPR-HCR platform, when coupled with an upstream amplifier, achieves a practical sensitivity as low as similar to aM of ASFV gene in serum.
Article
Chemistry, Analytical
Linying Yu, Liping Zhu, Yao Peng, Mengting Sheng, Jianshe Huang, Xiurong Yang
Summary: A highly sensitive and versatile dual-amplification electrochemiluminescence (ECL) biosensing platform was developed for rapid and sensitive detection of biomarkers. The platform utilized DNA nanostructures and catalyzed hairpin assembly (CHA) to achieve high amplification efficiency. The biosensor demonstrated a detection limit of 1.09 fM for target DNA and showed selective detection of silver ions. The proposed strategy has potential applications in disease diagnosis and environmental monitoring.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yan Shan Ang, Lin-Yue Lanry Yung
Summary: This study reports the impact of fluorophore-quencher labelling on the thermodynamics of DNA hairpin opening and proposes two counter strategies to address this issue.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Ni Yang, Yongfeng Wei, Xiaofeng Kang, Zhuoqun Su
ANALYTICAL METHODS
(2015)
Article
Chemistry, Analytical
Yongfeng Wei, Zhuoqun Su, Xiao-feng Kang, Yanli Guo, Xiaoxue Mu
Article
Chemistry, Analytical
Zhuoqun Su, Yongfeng Wei, Xiao-feng Kang
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Fujun Yao, Xiao Peng, Zhuoqun Su, Lei Tian, Yanli Guo, Xiao-feng Kang
ANALYTICAL CHEMISTRY
(2020)
Article
Agriculture, Multidisciplinary
Xianlong Zhang, Guoliang Li, Jianghua Liu, Zhuoqun Su
Summary: A novel nanozyme-enhanced SERS immunoassay platform was successfully developed, utilizing a peroxidase-mimicking nanozyme to replace natural enzymes for the detection of allergy proteins. This platform showed improved accuracy, high sensitivity, and good selectivity in detecting alpha-lactalbumin, with a low detection limit of 0.01 ng/mL, and demonstrated satisfactory results in food samples, providing insights into the use of nanozymes in analytical methods and food safety assays.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Agriculture, Multidisciplinary
Zhuoqun Su, Tong Li, Di Wu, Yongning Wu, Guoliang Li
Summary: Rapid and sensitive detection technologies for food contaminants are crucial for ensuring food safety. Traditional methods often struggle with accuracy, sensitivity, and specificity issues due to the complexity of food samples and trace amount distributions. Over the past decades, single-molecule detection (SMD) has emerged as a promising approach to achieve rapid and ultrasensitive measurements with minimal sample consumption, particularly in the field of food contaminants detection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)