Article
Chemistry, Multidisciplinary
Lorena Simon-Gracia, Kristina Kiisholts, Vilma Petrikaite, Allan Tobi, Merli Saare, Prakash Lingasamy, Maire Peters, Andres Salumets, Tambet Teesalu
Summary: The study explores the use of homing peptides for precision delivery of diagnostic and therapeutic compounds to endometriotic lesions. Findings indicate that PL1 peptide can bind to endometriotic cells, showing potential for precision diagnosis and therapy through PL1-guided nanoparticles.
Article
Pharmacology & Pharmacy
Xiaoxu Li, Ximing Pu, Xingming Wang, Juan Wang, Xiaoming Liao, Zhongbin Huang, Guangfu Yin
Summary: The blockage of the blood-brain barrier (BBB) and the lack of specific targeting remain major obstacles and challenges in targeted nano-pharmaceutical therapy for glioblastoma (GBM). In this study, a dual-targeting ligand for GBM was screened using a combination of phage display peptide library biopanning and affinity-adaptability analysis. The optimized WTW peptide showed high affinity to U251-MG cells and moderate affinity to bEnd.3 cells, allowing for receptor-mediated internalization, transport, and specific binding to GBM cells. In vitro and in vivo experiments demonstrated that WTW peptide had improved BBB penetration efficiency and GBM accumulation compared to other peptides tested. The screened WTW peptide has the potential to advance GBM targeted therapy.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Man Hu, Fangyu Wang, Ning Li, Guangxu Xing, Xuefeng Sun, Yunshang Zhang, Shuai Cao, Ningning Cui, Gaiping Zhang
Summary: Gram-positive enhancer matrix (GEM) nanoparticles have been widely used in mucosal immunity and subunit vaccine preparation due to their good immunological activities. In this study, a high affinity peptide ligand PL23 was designed and screened out using virtual screening technology, which could specifically target a non-epitope region of Classic Swine Fever Virus (CSFV) E2 protein. The conjugation of PL23 with GEM nanoparticles showed promising results in promoting antigen uptake by antigen-presenting cells and inducing immune responses without producing inflammasomes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Medicine, Research & Experimental
Xiancheng Zhang, Xiaoyu Zhang, Huiling Gao, Guangyan Qing
Summary: This article introduces the application of phage display technology in Alzheimer's disease research. Phage display is a powerful platform that allows selection and identification of drug candidates with high affinities and specificities. The article summarizes the basic principles and applications of phage display, as well as its potential roles in Alzheimer's disease therapy, targeted drug delivery, and early detection.
Article
Biochemistry & Molecular Biology
K. M. Koss, T. Son, C. Li, Y. Hao, J. Cao, M. A. Churchward, Z. J. Zhang, J. A. Wertheim, R. Derda, K. G. Todd
Summary: Microglia play critical roles in the development and healthy function of the brain and spinal cord, but they are also involved in the pathology of various neuropsychiatric disorders. In this study, researchers explored the functional phenotypes of microglia and mixed microglia/astrocytes using in vitro models. They hypothesized that the biomolecular profile of these cells undergoes phenotypical changes, and they searched for novel peptide binders using a custom phage library. By studying the secretion of cytokines and nitric oxide and analyzing the expression of inducible nitric oxide synthase, they identified 58 unique peptides with potential functions related to glial support, inflammation activation, and regulation in neurodegenerative and neuropsychiatric disorders.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Ji Hong Kim, Chae Hwan Cho, Jae Hwan Shin, Jin Chul Yang, Tae Jung Park, Jinyoung Park, Jong Pil Park
Summary: An affinity peptide specific to the influenza H5N1 virus neuraminidase (NA) was successfully identified via phage display technique and developed into an electrochemical biosensing system. The BSA/MXene nanocomposite-based peptide sensor displayed significant dissociation constant and detection limit, with good reproducibility, stability, and recovery in the presence of spiked human plasma. This study provides an alternative bioanalytical sensing platform for more sensitive virus detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Cell & Tissue Engineering
JongMyung Kim, Chunhua She, Marine Potez, Ping Huang, Qiulian Wu, Briana C. Prager, Zhixin Qiu, Shideng Bao, Jeremy N. Rich, James K. C. Liu
Summary: This study utilized phage display techniques to isolate a peptide, EYA1, with specific binding properties to GSCs, which are preferentially expressed in GSCs compared to differentiated tumor progeny. Interaction between EYA1 and MYC was identified, targeting EYA1 led to decreased proliferation, migration, and self-renewal of GSCs as well as reduced tumor growth.
Article
Multidisciplinary Sciences
Junxia Wang, Liang Li, Yingkun Zhang, Kaifeng Zhao, Xiaofeng Chen, Haicong Shen, Yuanqiang Chen, Jia Song, Yuqiang Ma, Chaoyong Yang, Hongming Ding, Zhi Zhu
Summary: Efficient molecular selection is crucial for various biomedical applications. By utilizing deterministic lateral-displacement microfluidics, the equilibrium of dissociation can be greatly shifted, leading to improved selection efficiency and better discrimination of ligand affinity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Virology
Aitthiphon Chongchai, Sajee Waramit, Tunchanok Wongwichai, Jirawan Kampangtip, Thanyaluck Phitak, Prachya Kongtawelert, Amin Hajitou, Keittisak Suwan, Peraphan Pothacharoen
Summary: The study developed a targeted delivery strategy which successfully delivered transgenes to HACs using CAP.Phage particles, with the CAP ligand demonstrating preference for binding to pathogenic chondrocytes.
Article
Chemistry, Medicinal
Rushdhi Rauff, Sudeshi M. Abedeera, Stefani Schmocker, Jiale Xie, Sanjaya C. Abeysirigunawardena
Summary: This study discovered a 12-mer peptide that specifically binds to m(6)A-modified RNA and inhibits the demethylase enzyme FTO. Treatment with this peptide, called mtp1, increased m(6)A levels and decreased cell viability in cancer cell lines, suggesting its potential as a cancer drug.
Article
Nanoscience & Nanotechnology
Ji Hong Kim, Hye-Seon Jeong, Jaehyeon Hwang, Dae-Hyuk Kweon, Chang-Hyung Choi, Jong Pil Park
Summary: A sensor based on affinity peptide-immobilized hydrogel microspheres has been developed for the selective detection of influenza A virus. The sensor shows rapid and accurate detection capability, which is crucial for the effective control of pandemic flu outbreaks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Ni-Yuan Zhang, Da-Yong Hou, Xing-Jie Hu, Jian-Xiao Liang, Man-Di Wang, Zhang-Zhi Song, Li Yi, Zhi-Jia Wang, Hong-Wei An, Wanhai Xu, Hao Wang
Summary: Reported here is an intracellular fabricated Nano-PROTACs modality with a center-spoke degradation network, which achieves efficient dose-dependent protein degradation in tumor. Nano-PROTACs are self-assembled by intermolecular hydrogen bond interactions triggered by higher GSH concentrations inside tumor cells, forming fibrous structures that can form effective polynary complexes and E3 ligases degradation network. Nano-PROTACs demonstrate dose-dependent protein degradation of variable proteins of interest (POI) in vitro, with a degradation rate of 95% and long-lasting potency up to 72 h. In vivo, Nano-PROTACs achieve dose-dependent protein degradation up to 79% and tumor growth inhibition in xenograft mouse models.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Hawk Leong Yuen, Szn Yi Chan, Yi En Ding, Suxing Lim, Gim Cheong Tan, Chiew Ling Kho
Summary: This study aimed to develop a potent antibacterial peptide (ABP) with minimal toxicity through phage display selection and computer-assisted modification. A peptide called PAM-5 with enhanced antibacterial features was obtained by modifying the peptide selected for its high affinity to Pseudomonas aeruginosa cell surface using an online peptide database. PAM-5 showed activity against various Gram-negative and selected Gram-positive bacteria, and was stable in human plasma without toxic effects on human cells and erythrocytes.
Review
Microbiology
Weronika Jaroszewicz, Joanna Morcinek-Orlowska, Karolina Pierzynowska, Lidia Gaffke, Grzegorz Wegrzyn
Summary: Phage display technology is a widely applicable method in biotechnology, recognized by the Nobel Prize. This article provides an overview of the technology, including comparisons of different display systems, their advantages and disadvantages, and examples of applications in various fields. It also discusses other peptide display technologies and highlights the ongoing development and improvements in these methods, offering new solutions in the future.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Medicine, General & Internal
Menglong Xu, Gaoxin Lei, Manman Chen, Ke Wang, Wenxiu Lv, Panpan Zhang, Tuo Hu, Jie Gao, Chenchen Lu, Ying Mei, Zhipan Xu, Zhengli Bai, Huajing Hu, Yiwei Jiang, Shuhua Tan
Summary: A fully human antibody FAP2M21 with potent inhibitory effect on PCSK9 was generated, showing promise as a therapeutic agent for treating hypercholesterolemia and associated cardiovascular diseases.
Article
Clinical Neurology
Martin N. Stienen, Jiri Bartek, Marcus A. Czabanka, Christian F. Freyschlag, Angelos Kolias, Sandro M. Krieg, Wouter Moojen, Mirjam Renovanz, Nicolas Sampron, Sasan D. Adib, Gerrit A. Schubert, Andreas K. Demetriades, Florian Ringel, Luca Regli, Karl Schaller, Torstein R. Meling
ACTA NEUROCHIRURGICA
(2019)
Article
Engineering, Biomedical
Tonu Ratsep, Toomas Asser
CLINICAL BIOMECHANICS
(2019)
Article
Chemistry, Multidisciplinary
Pille Saalik, Prakash Lingasamy, Kadri Toome, Ignacio Mastandrea, Liat Rousso-Noori, Allan Tobi, Lorena Simon-Gracia, Hedi Hunt, Paarn Paiste, Venkata Ramana Kotamraju, Gabriele Bergers, Toomas Asser, Tonu Ratsep, Erkki Ruoslahti, Rolf Bjerkvig, Dinorah Friedmann-Morvinski, Tambet Teesalu
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Multidisciplinary Sciences
Artur Vetkas, Ele Prans, Sulev Koks, Tonu Ratsep, Toomas Asset
SCIENTIFIC REPORTS
(2020)
Article
Clinical Neurology
Artur Vetkas, Ele Prans, Sulev Koks, Tonu Ratsep, Toomas Asser
Article
Biotechnology & Applied Microbiology
Toini Pemmari, Larisa Ivanova, Ulrike May, Prakash Lingasamy, Allan Tobi, Anja Pasternack, Stuart Prince, Olli Ritvos, Shreya Makkapati, Tambet Teesalu, Mitchell S. Cairo, Tero A. H. Jarvinen, Yanling Liao
Article
Multidisciplinary Sciences
Prakash Lingasamy, Allan Tobi, Kaarel Kurm, Sergei Kopanchuk, Aleksander Sudakov, Markko Salumae, Tonu Ratsep, Toomas Asser, Rolf Bjerkvig, Tambet Teesalu
SCIENTIFIC REPORTS
(2020)
Article
Clinical Neurology
Martin N. Stienen, Christian F. Freyschlag, Karl Schaller, Torstein Meling
ACTA NEUROCHIRURGICA
(2020)
Article
Oncology
Prakash Lingasamy, Anett-Hildegard Laarmann, Tambet Teesalu
Summary: This study investigated the effect of C-terminal genetic fusion of the iROD peptide to recombinant anti-TNC-C single-chain antibody on systemic tumor homing and extravasation. The results showed that iROD fusion enhanced the antibody's binding ability to alpha V beta 3 integrins, and iRGD fusion improved the antibody's targeting, extravasation, and penetration of minor blood vessels and parenchyma in U87-MG mice.
CURRENT CANCER DRUG TARGETS
(2021)
Article
Multidisciplinary Sciences
Liat Rousso-Noori, Ignacio Mastandrea, Shauli Talmor, Tova Waks, Anat Globerson Levin, Maarja Haugas, Tambet Teesalu, Luis Alvarez-Vallina, Zelig Eshhar, Dinorah Friedmann-Morvinski
Summary: Chimeric antigen receptor (CAR) T cell therapy targeting p32 shows promising anti-tumor responses by specifically recognizing and eliminating glioma cells and tumor derived endothelial cells in preclinical models. This may serve as a therapeutic option for glioblastoma patients.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Lorena Simon-Gracia, Kristina Kiisholts, Vilma Petrikaite, Allan Tobi, Merli Saare, Prakash Lingasamy, Maire Peters, Andres Salumets, Tambet Teesalu
Summary: The study explores the use of homing peptides for precision delivery of diagnostic and therapeutic compounds to endometriotic lesions. Findings indicate that PL1 peptide can bind to endometriotic cells, showing potential for precision diagnosis and therapy through PL1-guided nanoparticles.
Article
Pharmacology & Pharmacy
Prakash Lingasamy, Kristina Posnograjeva, Sergei Kopanchuk, Allan Tobi, Ago Rinken, Ignacio J. General, Eliana K. Asciutto, Tambet Teesalu
Summary: The study elucidates the binding dynamics of the PL1 peptide with its receptors, as well as the process of binding and internalization of PL1 nanoparticles in vitro. Results indicate that the engagement of PL1 peptide with FN-EDB and TNC-C is primarily driven by electrostatic interactions, and PL1-functionalized nanoparticles can deposit on the target ECM and induce cellular uptake.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.