Article
Cell Biology
Jie Zhang, Jiajun Tang, Jie Liu, Bo Yan, Bin Yan, Minjun Huang, Zhongmin Zhang, Liang Wang
Summary: This study demonstrated that melatonin promotes heterotopic ossification (HO) through regulating endothelial-mesenchymal transition (EndMT) in injured Achilles tendons in rats, providing potential directions for HO management.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Dong Mao, Jingyi Mi, Xiaoyun Pan, Gang Zhao, Yongjun Rui
Summary: Through this study, we demonstrated the potential of Galunisertib as a prophylactic drug for the treatment of traumatic HO or other related diseases triggered by over-expressed TGF-beta.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell & Tissue Engineering
L. Liu, Z. Li, S. Chen, H. Cui, X. Li, G. Dai, F. Zhong, W. Hao, K. Zhang, H. Liu
Summary: This study found that BRD4 plays a role in promoting bone formation in heterotopic ossification (HO), and the BRD4-Mancr signaling pathway is involved in the modulation of new bone formation in HO. Additionally, inhibition of BRD4 by JQ1 significantly attenuated pathological bone formation in the HO mouse model.
BONE & JOINT RESEARCH
(2021)
Article
Cell Biology
Xiaoxiao Han, Changhe Gao, Weicheng Lu, Jianfei Yan, Haoqing Xu, Zhenxing Guo, Wenpin Qin, Naining Lu, Jialu Gao, Weiwei Zhu, Yutong Fu, Kai Jiao
Summary: Heterotopic ossification (HO) severely affects people's lives, but its pathological mechanism remains unclear. This study found that extracellular DNA (ecDNA) from M2 macrophages initiates pathological calcification in HO and deoxyribonuclease can reverse this calcification.
Review
Cell & Tissue Engineering
Lukasz Pulik, Bartosz Mierzejewski, Aleksandra Sibilska, Iwona Grabowska, Maria Anna Ciemerych, Pawel Legosz, Edyta Brzoska
Summary: Heterotopic ossification is the formation of bone in non-osseous tissues, and it can be caused by genetic or non-genetic factors. TGF beta 1, BMPs, activin A, and other factors are known to induce HO. Dysregulation of noncoding RNAs may also play a role in the development of HO.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Gang Luo, Ziyang Sun, Hang Liu, Zhengqiang Yuan, Wei Wang, Bing Tu, Juehong Li, Cunyi Fan
Summary: In this study, it was found that verteporfin effectively inhibits trauma-induced heterotopic ossification formation by suppressing angiogenesis and osteogenesis. The YAP/beta-catenin signaling axis was downregulated by verteporfin, and lithium chloride reversed the inhibitory effects. Histological analysis and Micro-CT scan confirmed the therapeutic effect of verteporfin in a murine burn/tenotomy model.
Review
Cell Biology
Yili Xu, Mei Huang, Wenzhen He, Chen He, Kaixuan Chen, Jing Hou, Min Huang, Yurui Jiao, Ran Liu, Nanyu Zou, Ling Liu, Changjun Li
Summary: In this article, the primary characteristics of heterotopic ossification are reviewed from both clinical and basic research perspectives, with a special highlight on the influence of mechanics on heterotopic ossification, which serves an important role in the prophylaxis and treatment of HO.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Juehong Li, Ziyang Sun, Gang Luo, Shuo Wang, Haomin Cui, Zhixiao Yao, Hao Xiong, Yunwei He, Yun Qian, Cunyi Fan
Summary: The study showed increased accumulation of monocyte/macrophages and mast cells during the progression of HO, and depletion of macrophages and stabilization of mast cells significantly hindered HO formation. Quercetin was found to inhibit immune cell recruitment and inflammation, with potential for preventing trauma-induced HO by modulating SIRT1/NF kappa B signaling.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Endocrinology & Metabolism
Guorui Cao, Shaoyun Zhang, Yixuan Wang, Songtao Quan, Chen Yue, Junna Yao, Peter G. Alexander, Honglue Tan
Summary: Heterotopic ossification (HO) refers to the formation of extraskeletal bone in skeletal muscle and surrounding soft tissues. Acquired HO is often caused by restricted range of motion, explosion injury, nerve injury, or burns, leading to pain and limited joint activity. The specific mechanisms and cell precursors of HO are still not well understood, and effective methods to prevent or reduce HO formation are lacking. This review provides an update on the known risk factors and cellular origins of HO, focusing on the role of mesenchymal stem cells (MSCs) in acquired HO and discussing the latest therapeutic implications. The review highlights the current knowledge gaps and identifies potential targets for the prevention and treatment of acquired HO.
Article
Cell & Tissue Engineering
Ji-Hye Yea, Mario Gomez-Salazar, Sharon Onggo, Zhao Li, Neelima Thottappillil, Masnsen Cherief, Stefano Negri, Xin Xing, Qizhi Qin, Robert Joel Tower, Chen-Ming Fan, Benjamin Levi, Aaron W. James
Summary: Heterotopic ossification (HO) is a pathological process involving abnormal bone formation in synovial lined tissues. Using lineage tracing and single-cell RNA sequencing, it was found that synovial/tendon sheath progenitor cells contribute to HO and undergo aberrant osteochondral differentiation. TPPP3 is identified as an early progenitor cell marker in this process.
Review
Biochemistry & Molecular Biology
Ke Wang, Chi Ma, Jian Q. Feng, Yan Jing
Summary: This article explores the key role of cell transdifferentiation in skeletal development and diseases, focusing specifically on the transdifferentiation of chondrocytes to bone cells and the novel roles of tendon cells and adipocytes in skeletal formation and diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Zheng Zhang, Minghui Wang, Youli Zheng, Yanmei Dai, Jiashu Chou, Xiaowei Bian, Pengcheng Wang, Changyi Li, Jing Shen
Summary: This study found that miR-223 is upregulated in periodontitis and downregulated during the osteogenesis of PDL-derived cells. Overexpression of miR-223 inhibits osteogenic differentiation of PDL-derived cells, potentially by targeting the growth factor receptor genes FGFR2 and TGFβR2.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Materials Science, Biomaterials
Jingchen Xu, Yao Lin, Mi Tian, Xinyi Li, Yuanyuan Yin, Qiming Li, Ziyu Li, Jialiang Zhou, Xiaoge Jiang, Yulin Li, Song Chen
Summary: The study found that PDLSC-derived EVs play a positive role in tension-induced osteogenesis, enhancing the efficiency of orthodontic tooth movement (OTM) and promoting osteogenesis in bone cells. Additionally, mechano-sensitive miRNAs enriched in EVs may play a key role in promoting osteogenesis-related signaling pathways.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Juehong Li, Xin Wang, Zhixiao Yao, Feng Yuan, Hang Liu, Zhenyu Sun, Zhengqiang Yuan, Gang Luo, Xiangyun Yao, Haomin Cui, Bing Tu, Ziyang Sun, Cunyi Fan
Summary: It is discovered that pyroptotic macrophages induce senescence of tendon-derived stem cells (TDSCs) to promote osteogenic healing during trauma-induced heterotopic ossification (HO). Blocking pyroptosis reduces senescence and HO formation. The study provides insights into the aberrant regeneration-based theory for HO formation and facilitates the development of therapeutic strategies.
Article
Orthopedics
Roland E. Willburger, Friederike Brinkhoff, Jan Nottenkamper, Jan Krapp, Stella Oberberg
Summary: The formation of HO is completed three years after THA, and after this point, HO will not further increase. If HO occurs or increases after more than three years postoperatively, other triggering causes should be considered.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Genetics & Heredity
Kathleen Q. Schulte, F. Curtis Hewitt, Tara E. Manley, Andrew J. Reed, Maryam Baniasad, Nicolette C. Albright, Megan E. Powals, Danielle S. LeSassier, Alan R. Smith, Liwen Zhang, Leah W. Allen, Benjamin C. Ludolph, Katharina L. Weber, August E. Woerner, Michael A. Freitas, Myles W. Gardner
Summary: This study presents a simple workflow for the collection, enrichment, and fractionation of DNA and protein in touch samples, allowing for analysis of both components using traditional DNA casework methods and liquid chromatography-tandem mass spectrometry-based proteomics. While DNA in touch samples poses challenges due to its low abundance and potential degradation, proteins offer a robust alternative with stable quantities across various donors and fingerprint ages. This supports the future application of genetically variable peptide analysis for forensic identification in touch samples.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2021)
Article
Gastroenterology & Hepatology
Zobeida Cruz-Monserrate, Kristyn Gumpper, Sabrina Kaul, Niharika Badi, Samantha Terhorst, Kelly Dubay, Gregory B. Lesinski, William Fisher, Amy McElhany, Luis F. Lara, Somashekar Krishna, Thomas Mace, Natalia Higuita-Castro, Lilibeth Ortega-Pineda, Michael A. Freitas, Alice Hinton, Dhiraj Yadav, Phil A. Hart, Stephen J. Pandol, Saima Ahmed, Benoit Fatou, Hanno Steen, Darwin L. Conwell
Summary: Endoscopic pancreatic function tests can provide a reliable source for biomarkers of pancreatic disorders, but the integrity and quality of proteins and nucleic acids in pancreatic fluid samples can be influenced by preservation methods and storage time. Adding protease inhibitors immediately after collection and processing the fluid within 4 hours helps maintain the integrity of proteins and nucleic acids for downstream molecular analyses.
Article
Cardiac & Cardiovascular Systems
Anuradha Kalyanasundaram, Ning Li, Miranda L. Gardner, Esthela J. Artiga, Brian J. Hansen, Amy Webb, Michael A. Freitas, Maciej Pietrzak, Bryan A. Whitson, Nahush A. Mokadam, Paul M. L. Janssen, Peter J. Mohler, Vadim V. Fedorov
Summary: This study found increased fibrosis and presence of myofibroblasts in the sinoatrial node (SAN) pacemaker complex of heart failure (HF) patients, with unique differences in gene and protein regulation compared to non-HF patients. Additionally, RNA sequencing and proteomic analyses identified distinctive profiles between SAN fibroblasts from HF and non-HF hearts, providing a comprehensive framework for further research on the role of SAN-specific fibrosis in cardiac rhythm regulation and arrhythmias.
Article
Engineering, Biomedical
Long Zhang, Miranda L. Gardner, Lakmal Jayasinghe, Michael Jordan, Julian Aldana, Nicolas Burns, Michael A. Freitas, Peixuan Guo
Summary: The study presents a label-free method for detecting single amino acid modifications in peptides using reengineered phi29 DNA packaging motor channels, enabling the differentiation of single or multiple modifications in the MinION (TM) system. This advancement represents a significant step towards developing a high-throughput detection platform for clinical diagnosis based on PTM utilizing nanopores.
Article
Biochemistry & Molecular Biology
Miranda L. Gardner, Michael A. Freitas
Summary: Careful consideration of missing value imputation is necessary in proteomics data analysis to address both MAR and MNAR conditions. Deciding when to impute data and selecting a method that reflects the combination of MAR and MNAR are essential considerations in performing missing value imputation. Further evaluation of missing value imputation strategies is needed to address the common MNAR problem in proteomics data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Maryam Baniasad, Andrew J. Reed, Stella M. Lai, Liwen Zhang, Kathleen Q. Schulte, Alan R. Smith, Danielle S. LeSassier, Katharina L. Weber, F. Curtis Hewitt, August E. Woerner, Myles W. Gardner, Vicki H. Wysocki, Michael A. Freitas
Summary: The study optimized protein extraction and digestion methods for mass spectrometry-based proteomics analysis of human skin samples. By systematically varying digestion parameters, the study aimed to enhance repeatability and increase the identification of peptides and proteins, particularly genetically variable peptides (GVPs). The research demonstrated the potential for forensic laboratories to employ proteomics sample preparation for GVP analysis to facilitate human identification, complementing traditional DNA profiling methods.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Liudmila Popova, Prabakaran Nagarajan, Callie M. Lovejoy, Benjamin D. Sunkel, Miranda L. Gardner, Meng Wang, Michael A. Freitas, Benjamin Z. Stanton, Mark R. Parthun
Summary: In this study, it was found that the loss of HAT1 during replication resulted in inaccessibility of specific heterochromatin regions, which make up a significant portion of the genome. Additionally, HAT1 was shown to have a global repression effect on H3 K9 me3 levels and overlap with a subset of Lamin-Associated Domains.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Aleksandra Adamovich, Mariame Diabate, Tapahsama Banerjee, Gregory Nagy, Nahum Smith, Kathryn Duncan, Erika Mendoza Mendoza, Gisselle Prida, Michael A. Freitas, Lea M. Starita, Jeffrey D. Parvin
Summary: Pathogenic variants in BRCA1 are associated with an increased risk of hereditary breast and ovarian cancer. This study used functional assays to evaluate a large number of BRCA1 variants, finding consistent results in terms of repair function and drug resistance. These results are valuable for characterizing variants that are individually detected too infrequently in the population.
AMERICAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Multidisciplinary Sciences
Matthew S. Conrad, Miranda L. Gardner, Christine Miguel, Michael A. Freitas, Kara M. Rood, Marwan Ma'ayeh
Summary: Fetal growth restriction and pre-eclampsia alter the structure and composition of the umbilical cord, with significant differences in proteins related to extracellular matrix, cellular process, inflammation, and angiogenesis pathways. Further research on these protein differences may help identify therapeutic targets for FGR and FGR with PreE.
Article
Biochemical Research Methods
Gordon T. Luu, Michael A. Freitas, Itzel Lizama-Chamu, Catherine S. McCaughey, Laura M. Sanchez, Mingxun Wang
Summary: In this paper, we present TIMSCONVERT, a high-throughput data conversion workflow that converts timsTOF Pro/fleX mass spectrometer raw data files to mzML and imzML formats while incorporating ion mobility data and maintaining compatibility with popular data analysis tools.
Article
Genetics & Heredity
August E. Woerner, Benjamin Crysup, F. Curtis Hewitt, Myles W. Gardner, Michael A. Freitas, Bruce Budowle
Summary: Forensic genetic investigations often rely on DNA analysis, but when the DNA is limited, protein can be used as an alternative target. However, interpreting protein polymorphisms in a forensic context requires addressing certain complexities. This study presents a novel approach that converts genomic information into proteomic information to overcome these complexities. The results show that this method performs well in simulated samples.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2022)
Article
Orthopedics
Katelynn M. Donnelly, Hannah G. Theriot, John P. Bourgeois, Andrew G. Chapple, Peter C. Krause, Vinod Dasa
Summary: A systematic review of randomized controlled trials on total knee arthroplasty and total hip arthroplasty publications reveals a significant lack of inclusion of social determinants of health (SDOH) variables, such as race, ethnicity, insurance, income, and education. The exclusion of these variables may indirectly perpetuate disparities if research using non-representative patient samples is used in shaping health policies and national standards.
JOURNAL OF ARTHROPLASTY
(2023)
Article
Orthopedics
Leland van Deventer, Amy Bronstone, Claudia Leonardi, Matthew Bennett, Peter Yager, Vinod Dasa
Summary: Changing TKA discharge opioid prescriptions to upon request instead of automatic distribution can significantly reduce opioid use without compromising pain control, resulting in more opioid-free recoveries, particularly among opioid-naive patients.
JOURNAL OF EXPERIMENTAL ORTHOPAEDICS
(2023)
Article
Orthopedics
Cody Crnkovic, Robert Quiring, Andrew G. Chapple, Amy Bronstone, Peter C. Krause, Vinod Dasa
Summary: Race, ethnicity, and socioeconomic status (SES) were infrequently reported and analyzed in articles published in two top orthopaedic journals. This problem may be remedied if orthopaedic journals impose standards for the reporting and analysis of patient demographics in studies with human cohorts.
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2022)
Meeting Abstract
Obstetrics & Gynecology
Matthew S. Conrad, Miranda L. Gardner, Christine Miguel, Michael Freitas, Kara M. Rood, Marwan Maayeh
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.
