Article
Multidisciplinary Sciences
Jesse Goyette, David Depoil, Zhengmin Yang, Samuel A. Isaacson, Jun Allard, P. Anton van der Merwe, Katharina Gaus, Michael L. Dustin, Omer Dushek
Summary: Protein-protein binding domains, such as the Src homology 2 (SH2) domains, play a critical role in signaling networks. Tandem SH2 domains can enhance binding affinity and specificity, but there is a trade-off between long-lived binding and rapid signaling reversal. This study shows that phosphatases can accelerate the unbinding rate of tandem SH2 domains, breaking the trade-off and allowing for efficient T cell antigen discrimination.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Timothy F. Walseth, Andreas H. Guse
Summary: Nicotinic acid adenine dinucleotide 2'-phosphate (NAADP) is a naturally occurring nucleotide that has been shown to be involved in the release of Ca2+ from intracellular stores. The identity of the NAADP receptor remains unknown, but recent studies have identified HN1L/JPT2 as a high affinity NAADP binding protein that is essential for modulating Ca2+ channels. This suggests that NAADP may act as a trigger for Ca2+ signaling in cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Nicholas Manolios, John Papaemmanouil, David Adams
Summary: T lymphocytes are crucial cells in the immune system, and ion channels play a critical role in regulating their function. This review summarizes the impact of ion channels on T cell function and disease, emphasizing their potential as drug targets.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mariam Ahmed Galal, Samhar Samer Alouch, Buthainah Saad Alsultan, Huda Dahman, Nouf Abdullah Alyabis, Sarah Ammar Alammar, Ahmad Aljada
Summary: This comprehensive review thoroughly explores the intricate involvement of insulin receptor isoforms and insulin-like growth factor receptors in the insulin and insulin-like growth factor signaling pathway, emphasizing their crucial roles in cancer development and resistance to anti-cancer drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Kogulan Yoganathan, Anqi Yan, Juliana Rocha, Ashton Trotman-Grant, Mahmood Mohtashami, Lisa Wells, Juan Carlos Zuniga-Pfluecker, Michele K. Anderson
Summary: The Alt domain in HEBAlt plays a critical role in integrating cytokine signaling and E protein activity. Phosphorylation of a unique YYY motif in the Alt domain can significantly increase HEBAlt activity, and this increase is dependent on the activity of Janus kinases. HEBAlt is sensitive to changes in cell type and signaling environment during T cell development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Sumit Kumar, Mark J. A. Schoonderwoerd, Jessie S. Kroonen, Ilona J. de Graaf, Marjolein Sluijter, Dina Ruano, Roman Gonzalez-Prieto, Matty Verlaan-de Vries, Jasper Rip, Ramon Arens, Noel F. C. C. de Miranda, Lukas J. A. C. Hawinkels, Thorbald van Hall, Alfred C. O. Vertegaal
Summary: The pharmacological inhibition of the SUMO pathway represents a potential strategy for targeting PDAC by inhibiting cancer cell cycle progression and activating anti-tumor immunity through interferon signaling. TAK-981, a novel small molecule inhibitor, has shown potential in reducing SUMOylation and inhibiting the proliferation of PDAC cells.
Article
Cell Biology
Michael Nicosia, Juyeun Lee, Ashley Beavers, Danielle Kish, George W. Farr, Paul R. McGuirk, Marc F. Pelletier, Justin D. Lathia, Robert L. Fairchild, Anna Valujskikh
Summary: Our study uncovers a novel function of aquaporin 4 (AQP4) in T lymphocytes, showing that AQP4 facilitates TCR signaling and T cell activation via the actin cytoskeleton. AQP4 deficiency or inhibition impairs actin cytoskeleton rearrangements following TCR crosslinking, resulting in inferior TCR polarization and a loss of TCR signaling. These findings highlight AQP4 as a potential therapeutic target for preventing TCR-mediated T cell activation.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Diana Gil, Bjorn-Philipp Diercks, Andreas H. Guse, Genevieve Dupont
Summary: Ca2+ signaling is crucial for T cell activation and the formation of microdomains that facilitate Ca2+ entry. A mathematical model was developed to investigate the dynamics of Ca2+ microdomains triggered by IP3 signaling and RYR activation. Simulations showed that the opening of RYRs and the influx of Ca2+ through ORAI1 channels contribute to the formation of microdomains during T cell stimulation. The model provides a tool for studying the development and interaction of Ca2+ microdomains in different states of T cell activation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Cell Biology
Odilia B. J. Corneth, Stefan F. H. Neys, Rudi W. Hendriks
Summary: This review discusses the importance of abnormal B cell signaling in autoimmune diseases, focusing on aberrant B cell receptor (BCR) signaling and other signal transduction pathways. Therapeutic strategies for interfering with B cell signal transduction are also briefly discussed.
Article
Oncology
Ling Wu, Joanna Brzostek, Shvetha Sankaran, Qianru Wei, Jiawei Yap, Triscilla Y. Y. Tan, Junyun Lai, Paul A. MacAry, Nicholas R. J. Gascoigne
Summary: Chimeric antigen receptors (CARs) redirect T cells effectively in combating cancers and have achieved great success in clinical practice. However, the mechanisms underlying CAR signaling and function are not fully understood. Research findings suggest that CAR and TCR exhibit distinct signaling characteristics, providing new insights for clinical behavior of CAR-T therapy and technology optimization.
Article
Endocrinology & Metabolism
Amrita Ahluwalia, Neil Hoa, Debbie Moreira, Daniel Aziz, Karanvir Singh, Khushin N. Patel, Ellis R. Levin
Summary: This study investigates the function of membrane estrogen receptor beta (ER beta) in opposing AngII-induced cardiac cell pathology. The results demonstrate that membrane ER beta plays a crucial role in mitigating cardiac cell pathology and provides insights into the mechanisms of cardiac hypertrophy and fibrosis.
Review
Oncology
Pengchao Zhang, Guizhong Zhang, Xiaochun Wan
Summary: Adoptive cell therapies (ACTs), including CAR-T cell therapy, have revolutionized the field of cancer treatment. However, CAR-T cell therapy still has limitations in practicality and toxicity. To overcome these challenges, CAR engineering technology has been applied to other types of immune cells, leading to the development of new cell therapies such as CAR-NK, CAR-macrophage, CAR-γδT, and CAR-NKT. This review discusses the development, advantages, and challenges of ACTs, as well as current strategies to maximize their therapeutic potential. It also explores the importance of gene transduction strategies in immune cell engineering and the potential of creating a positive feedback immune circuit for future cancer immunotherapy.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2023)
Review
Immunology
Raul M. Torres, Jacqueline A. Turner, Marc D'Antonio, Roberta Pelanda, Kimberly N. Kremer
Summary: This review discusses the role of the LPA-LPA(5) axis in peripheral immunological tolerance mechanisms and its disruption in chronic inflammation. LPA-LPA(5) signaling regulates effector cytotoxic CD8 T cells through two mechanisms and impairs tumor immunity and CD8 T cell killing in mouse models.
IMMUNOLOGICAL REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Jun-ichi Kashiwakura, Kenji Oritani, Tadashi Matsuda
Summary: Adaptor molecules play a crucial role in signal transduction in immune cells and are potential therapeutic targets for T cell-mediated immune disorders. STAP-2, a member of the STAP family, is involved in T cell activation and autoimmune diseases. Understanding the impact of STAP-2 on T cell-mediated inflammation and immune diseases can aid in the development of novel therapeutic strategies.
Article
Multidisciplinary Sciences
Rushita A. Bagchi, Emma L. Robinson, Tianjing Hu, Ji Cao, Jun Young Hong, Charles A. Tharp, Hanan Qasim, Kathleen M. Gavin, Julie Pires da Silva, Jennifer L. Major, Bradley K. McConnell, Edward Seto, Hening Lin, Timothy A. McKinsey
Summary: This article highlights the importance of lysine myristoylation in regulating protein localization and function, as well as its role in controlling G protein-coupled receptor signaling. Inhibiting HDAC11 may have therapeutic potential for manipulating adipocyte phenotypes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)