Article
Chemistry, Multidisciplinary
Marina Toplak, Adelheid Nagel, Britta Frensch, Thorsten Lechtenberg, Robin Teufel
Summary: We report a novel regulatory principle for natural product biosynthesis involving an acetyltransferase. The acetyltransferase modifies a redox-tailoring enzyme, enabling pathway furcation and alternating pharmacophore assembly in rubromycin polyketide biosynthesis. This discovery provides new perspectives for improving enzyme activities and natural product titers.
Article
Microbiology
Nayeon Ki, Inseong Jo, Yongseong Hyun, Jinwook Lee, Nam-Chul Ha, Hyun-Myung Oh
Summary: This study determines the crystal structure of the putative N-acetyltransferase of phage SPN3US and reveals its novel homodimeric arrangement. Further biochemical analyses demonstrate that the enzyme has a very narrow substrate specificity, highlighting the need for additional studies to elucidate its biochemical activity and the interaction between phage and host bacteria.
JOURNAL OF MICROBIOLOGY
(2022)
Review
Endocrinology & Metabolism
Bharti Jaiswal, Akanksha Agarwal, Ashish Gupta
Summary: This review summarizes recent research advances in understanding the role of lysine acetyltransferases (KATs) in regulating androgen receptor (AR) activity at the level of post-translational modifications in normal prostate physiology and prostate cancer (PCa) development. Targeting these enzymes may have therapeutic potential for AR-related pathology in combination with hormonal therapy.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Microbiology
Amuza Byaruhanga Lucky, Chengqi Wang, Ahmad Rushdi Shakri, Mohammad Kalamuddin, Anongruk Chim-Ong, Xiaolian Li, Jun Miao
Summary: This study demonstrates the critical role of PfGCN5 gene in stress responses of Plasmodium falciparum, indicating its potential as a target for malaria intervention.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemical Research Methods
Jennifer R. Fleming, Franziskus Hauth, Joerg S. Hartig, Olga Mayans
Summary: Members of the GNAT family, which are found in all domains of life, have diverse functions and targets. However, only a few 3D structures of GNAT proteins are known, thus the family is poorly characterized. The crystal structure of LcGNAT, a canavanine acetyltransferase, was determined and showed similarities with other GNAT superfamily members. Site-directed mutagenesis revealed the role of certain residues in the catalytic mechanism of LcGNAT.
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2023)
Review
Genetics & Heredity
Onyekachi E. Ononye, Michael Downey
Summary: This review summarizes the types and functions of posttranslational modifications that regulate Gcn5 in both yeast and human cells. Common themes, unanswered questions, and strategies for future research are also outlined.
Article
Microbiology
Xinning Liu, Yuanchang Liu, Guangjian Zhao, Yidan Zhang, Lu Liu, Juan Wang, Yifan Wang, Siyu Zhang, Xin Li, Dongliang Guo, Peng Wang, Ximing Xu
Summary: This study investigated the impact of NAT from Vibrio vulnificus on acetyl group transformation in arylamine antibiotics, focusing on its structure, enzyme activity, and stability, revealing its activity under different conditions and sensitivity to metal ions. Additionally, kinetic parameters and structural analysis showed that (VIBVN)NAT could acetylate various aromatic amine substrates and contribute to arylamine antibiotic resistance in Vibrio vulnificus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Lihua Yin, Weina Sun, Jing Xu, Xiwang Ke, Xiaodan Xu, Yongxia Guo, Yuhu Zuo
Summary: N-acetyltransferases, such as VaNATA1, are important in plant response to biotic and abiotic stresses. VaNATA1 in Vigna angularis is a member of the SSAT family of GNATs and acts as a positive regulator of the defense system during infection with Uromyces vignae.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Cell Biology
Jia Li, Chenghui Yan, Yilong Wang, Can Chen, Haibo Yu, Dan Liu, Kai Huang, Yaling Han
Summary: Pathological cardiac hypertrophy is a major risk factor for heart failure, characterized by abnormal remodeling of cardiomyocytes in response to pressure overload or other stress stimuli. The study reveals that GCN5 plays a significant role in promoting cardiac hypertrophy through excessive activation of the TAK1-JNK/p38 signaling pathway.
CELL DEATH & DISEASE
(2022)
Article
Infectious Diseases
Arthika Manoharan, Samantha Ognenovska, Denis Paino, Greg Whiteley, Trevor Glasbey, Frederik H. Kriel, Jessica Farrell, Kate H. Moore, Jim Manos, Theerthankar Das
Summary: NAC is a non-toxic, antibiofilm agent in vitro and can prevent cell invasion and IBC formation by uropathogens, providing a potentially novel and efficacious treatment for UTIs. When combined with an antibiotic, it may disrupt bacterial biofilms and eliminate residual bacteria.
Review
Immunology
Ailing Zhao, Jiazheng Sun, Yipin Liu
Summary: Bacterial biofilms are complex microbial communities surrounded by extracellular polymeric substances. They are a significant problem in treating bacterial infections and can exhibit increased resistance to antibiotics, causing device-related and non-device infections and posing a serious threat to global health. Therefore, early detection and search for new treatments are essential. This paper systematically reviews the formation, infections, detection methods, and potential treatment strategies of bacterial biofilms, aiming to provide researchers with the latest progress in their detection and treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
P. Shirmast, S. M. Ghafoori, R. M. Irwin, J. Abendroth, S. J. Mayclin, D. D. Lorimer, Thomas E. Edwards, Jade K. Forwood
Summary: GNAT enzymes play important roles in biological processes by catalyzing acetylation of various substrates. This study characterized the high-resolution structure of a GNAT enzyme bound with acetyl-CoA, revealing a new dimer interface and suggesting diverse oligomeric assemblies. The structure presented in this study can be utilized for future computational docking and structure-activity studies.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Cillian Variot, Daniel Capule, Xhulio Arolli, Jackson Baumgartner, Cory Reidl, Charles Houseman, Miguel A. Ballicora, Daniel P. Becker, Misty L. Kuhn
Summary: In this study, we investigated how the PA3944 enzyme from Pseudomonas aeruginosa recognizes different acceptor substrates and identified critical residues for substrate specificity. By mutating seven amino acid residues to alanine, we determined their effects on the kinetic parameters of PA3944. We found that certain residues improved the affinity and catalytic efficiency of PA3944 towards NANMO and/or polymyxin B. Additionally, one mutant (R106A) showed substrate inhibition towards NANMO, and we propose potential reasons for this inhibition based on further substrate docking studies with R106A.
Article
Biochemistry & Molecular Biology
Chunpeng An, Lei Deng, Huawei Zhai, Yanrong You, Fangming Wu, Qingzhe Zhai, Alain Goossens, Chuanyou Li
Summary: The plant hormone jasmonate (JA) regulates plant immunity and adaptive growth through a genome-wide transcriptional program. This study uncovers the reversible acetylation of the corepressor TPL and its role in JA signaling. The histone acetyltransferase GCN5 mediates TPL acetylation, promoting its interaction with the adaptor protein NINJA and recruitment to MYC2 target promoters for transcriptional repression. Conversely, the histone deacetylase HDA6 weakens TPL-NINJA interaction and inhibits TPL recruitment, facilitating transcriptional activation. The balance between GCN5 and HDA6 maintains TPL acetylation homeostasis, determining the transcriptional state of JA-responsive genes.
Article
Biology
Hai-Qing Liu, Zuo-Xian Pu, Dong-Wei Di, Ya-Jie Zou, Yu-Man Guo, Jun-Li Wang, Li Zhang, Peng Tian, Qiong-Hui Fei, Xiao-Feng Li, Allah Jurio Khaskheli, Lei Wu, Guang-Qin Guo
Summary: This study demonstrates the important role of the Arabidopsis NatB complex in the N-terminal acetylation of YUC proteins, maintaining the normal auxin homeostasis in plants.
COMMUNICATIONS BIOLOGY
(2022)