Review
Immunology
Yi Zhang, Zheng Li, Ying Huang, Bingwen Zou, Yong Xu
Summary: Combining tumor immunotherapy with nanoparticle-based hyperthermia has been found to enhance cancer treatment effectiveness. Tumor immunotherapy activates the immune system to attack cancer cells, while nanoparticle-based hyperthermia selectively kills cells by raising the temperature of tumor cells. The combination of both approaches can amplify anti-tumor responses, improve outcomes, and reduce side effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Matthew Bell, Stephen Gottschalk
Summary: CAR T cell therapy is effective for hematological malignancies, but there is a need to improve its efficacy for solid tumors and brain tumors. Several approaches are being pursued to enhance the antitumor activity of CAR T cells, including augmenting signal 3 of T cell activation and improving the function of CAR T cells in the tumor microenvironment.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ping Wen, Wei Wu, Feifan Wang, Hanqi Zheng, Ziyan Liao, Jiaqi Shi, Chaojie Zhu, Peng Zhao, Hao Cheng, Hongjun Li, Zhen Gu
Summary: Adoptive cell therapy (ACT) using allogeneic or autologous immune cells shows promise in targeted cancer therapy, but its efficacy against solid tumors is limited. This review summarizes various devices for cell delivery and discusses the perspectives and challenges of using these devices for cancer immunotherapy.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Immunology
Andrea M. Amitrano, Brandon J. Berry, Kihong Lim, Kyun-Do Kim, Richard E. Waugh, Andrew P. Wojtovich, Minsoo Kim
Summary: Cancer immunotherapy is more effective against hematological malignancies than solid tumors due to metabolic challenges in the tumor microenvironment. Mitochondria play a key role in CD8(+) T cell activation and effector function. Enhanced mitochondrial function is associated with improved T cell migration and effector function, highlighting the importance of mitochondrial metabolism in T cell responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Katherine A. Freitas, Julia A. Belk, Elena Sotillo, Patrick J. Quinn, Maria C. Ramello, Meena Malipatlolla, Bence Daniel, Katalin Sandor, Dorota Klysz, Jeremy Bjelajac, Peng Xu, Kylie A. Burdsall, Victor Tieu, Vandon T. Duong, Micah G. Donovan, Evan W. Weber, Howard Y. Chang, Robbie G. Majzner, Joaquin M. Espinosa, Ansuman T. Satpathy, Crystal L. Mackall
Summary: This research identified MED12 and CCNC as key genes controlling T cell function through CRISPR knockout screening. Knockout of MED12 gene enhances antitumor activity, prolongs the effector phenotype of CAR-T and TCR-T cells, and promotes the expansion of non-engineered T cells. The study reveals the importance of Mediator in T cell effector programming and provides a new target to enhance the potency of antitumor T cell responses.
Review
Biochemistry & Molecular Biology
Lusine Hovhannisyan, Carsten Riether, Daniel M. Aebersold, Michaela Medova, Yitzhak Zimmer
Summary: CAR T cell-based therapies have revolutionized the treatment of hematological malignancies. However, the treatment of solid tumors with CAR T cells remains challenging. Radiation therapy, in combination with immune checkpoint inhibitors, has shown promising results in clinical trials. Combining radiation therapy with CAR T cell therapy may overcome the limitations in solid tumor treatment. This review discusses the potential and risks of this combination in cancer patients.
Article
Cell Biology
Xia Liu, Celine L. Hartman, Lingyun Li, Carolyn J. Albert, Fusheng Si, Aiqin Gao, Lan Huang, Yangjing Zhao, Wenli Lin, Eddy C. Hsueh, Lizong Shen, Qixiang Shao, Daniel F. Hoft, David A. Ford, Guangyong Peng
Summary: The functional state of T cells is critical for effective antitumor immunity. T cell senescence driven by malignant tumor cells and regulatory T cells is a common feature in cancers, characterized by unbalanced lipid metabolism and accumulation of lipid droplets. Inhibition of group IVA phospholipase A(2) can reprogram effector T cell lipid metabolism, prevent T cell senescence, and enhance antitumor immunity and immunotherapy efficacy.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Ashanti Concepcion Uscanga-Palomeque, Ana Karina Chavez-Escamilla, Cynthia Aracely Alvizo-Baez, Santiago Saavedra-Alonso, Luis Daniel Terrazas-Armendariz, Reyes S. Tamez-Guerra, Cristina Rodriguez-Padilla, Juan Manuel Alcocer-Gonzalez
Summary: This paper reviews the latest developments in CAR-T cells in cancer treatment, including the structure and manufacturing methods of different generations and variants. The challenges and limitations of CAR-T technology in treating hematological and solid cancer are discussed, as well as the use of CAR technology in other immune cells. The paper concludes that CAR-T cells have the potential to treat not only cancer but also other chronic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Qiang Zeng, Zhigang Liu, Ting Niu, Chuan He, Ying Qu, Zhiyong Qian
Summary: Cellular immunotherapy involves reinjecting in vitro modified immunocytes into patients to target and kill tumor cells. Chimeric antigen receptor T cell (CAR-T) therapy, as a successful representative of tumor cellular immunotherapy, has shown promising results in treating relapsed/refractory hematological tumors. However, challenges such as the limited effectiveness in solid tumors, cytokine release syndrome (CRS) or CAR-T-related encephalopathy syndrome (CRES), off-target effects, and high costs remain. Nanotechnology offers advantages in CAR construction, T cell transfection, expansion, delivery, antitumor effects, and reducing toxicities. This review summarizes the nanotechnologies used in CAR-T immunotherapy and discusses the future directions and challenges of combining nanotechnologies with CAR-T immunotherapy.
CHINESE CHEMICAL LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Sebastian Wirsching, Michael Fichter, Maximiliano L. Cacicedo, Katharina Landfester, Stephan Gehring
Summary: Cancer is a major cause of death globally. The search for innovative therapies is a key focus in medical research. Vaccine strategies targeting tumor-associated antigens have not yet achieved significant success, possibly due to the tumor microenvironment and regulatory T cells hindering efficacy. ASPH, a potential therapeutic target, is overexpressed in various tumors but minimally expressed in normal tissues. Computer analysis predicts that ASPH has four peptide sequences capable of stimulating regulatory T cell activity. Removing these regulatory T cell epitopes increases effector T cell responses and decreases the overall number of regulatory T cells. These findings suggest that screening and eliminating potential regulatory T cell epitopes can improve the efficacy of new vaccine candidates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Biomedical
Hung-Wei Cheng, Hsin-Yi Tsao, Chih-Sheng Chiang, San-Yuan Chen
Summary: Magnetic nanoparticles (MNPs) serve as a versatile theranostic tool for targeted delivery of immunotherapeutics and monitoring of cell/tissue responses. By utilizing magnetic properties for navigation and hyperthermia, MNPs enhance the efficacy of immunotherapy. The multimodal approach induces robust immune responses, minimizes safety issues, and allows simultaneous monitoring of immune activities.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Zhongyuan Guo, Ilkoo Noh, Audrey T. T. Zhu, Yiyan Yu, Weiwei Gao, Ronnie H. H. Fang, Liangfang Zhang
Summary: Cell membrane-based nanovaccines incorporating adjuvants have shown promising features for antitumor vaccination. This study presents cellular nanodiscs made from cancer cell membranes and a lipid-based adjuvant. The nanodiscs are efficiently taken up by antigen-presenting cells and stimulate the immune system, leading to effective control of tumor growth in both prophylactic and therapeutic settings, especially when combined with checkpoint blockades. These multiantigenic nanovaccines hold potential for activating antitumor immune responses in a wide range of cancers.
Article
Immunology
Gamze Gulden, Berranur Sert, Tarik Teymur, Yasin Ay, Nulifer Neslihan Tiryaki, Abhinava K. Mishra, Ercument Ovali, Nevzat Tarhan, Cihan Tastan
Summary: The development of genetic modification techniques has opened up a new era in cancer treatment by increasing the effectiveness and stability of CAR-T cell therapy. This study explores the use of Phytohemagglutinin (PHA) to increase the population of T cell memory phenotype in CAR-T cells, leading to long-term and efficient production of CAR-T cells.
Review
Medicine, General & Internal
Ella S. Atsavapranee, Margaret M. Billingsley, Michael J. Mitchell
Summary: Genetic engineering has transformed cancer immunotherapy by modifying primary T cells to enhance their therapeutic potential, with studies and clinical trials supporting the effectiveness of this approach.
Review
Oncology
Damie J. Juat, Stephanie J. Hachey, John Billimek, Michael P. Del Rosario, Edward L. Nelson, Christopher C. W. Hughes, Jason A. Zell
Summary: Colorectal cancer is the second leading cause of cancer-related deaths in the US. Adoptive T-cell therapy (ACT), leveraging the body's own immune system to recognize and target cancer, has gained popularity. This review summarizes the current data on the efficacy and safety of ACT in advanced CRC.
Article
Nanoscience & Nanotechnology
Yadileiny Portilla, Sara Mellid, Alberto Paradela, Antonio Ramos-Fernandez, Neus Daviu, Laura Sanz-Ortega, Sonia Perez-Yague, Maria P. Morales, Domingo F. Barber
Summary: In the study, it was found that different coatings did not affect the composition of the protein corona on iron oxide nanoparticles. Enzymes with hydrolase activity in the corona may explain the degradation of coatings when in contact with biological media. Coatings primarily determine the endocytic pathways used for nanoparticle internalization by cells.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Biomedical
Yadileiny Portilla, Vladimir Mulens-Arias, Alberto Paradela, Antonio Ramos-Fernandez, Sonia Perez-Yague, M. Puerto Morales, Domingo F. Barber
Summary: This study investigated the intracellular transit of two magnetic nanoparticles (MNPs) with different surface coatings in mouse cell lines. The results showed that the coating type influenced the intracellular trafficking rate and the nature of the endolysosomes in macrophages. The MNPs with a specific coating had a slower transit rate and resulted in endolysosomes with more lytic enzymes and catalytic proteins in macrophages. These MNPs also induced more autophagic vesicles and enhanced the expression of iron metabolism-related genes and proteins.
Article
Biotechnology & Applied Microbiology
Marta L. DeDiego, Yadileiny Portilla, Neus Daviu, Dario Lopez-Garcia, Laura Villamayor, Vladimir Mulens-Arias, Jesus G. Ovejero, Alvaro Gallo-Cordova, Sabino Veintemillas-Verdaguer, M. Puerto Morales, Domingo F. Barber
Summary: This study analyzed the potential of using iron oxide nanoparticles to treat and prevent SARS-CoV-2 infection. The nanoparticles were found to impair virus replication and transcription, both before and after infection, and SARS-CoV-2 infection was found to affect cellular iron metabolism. These findings suggest that the nanoparticles may be repurposed as prophylactic or therapeutic treatments for SARS-CoV-2 infection.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yadileiny Portilla, Yilian Fernandez-Afonso, Sonia Perez-Yague, Vladimir Mulens-Arias, M. Puerto Morales, Lucia Gutierrez, Domingo F. Barber
Summary: This study analyzed the biodistribution, organ accumulation and degradation of different coatings of iron oxide magnetic nanoparticles (MNPs) in vivo. The results showed that the coating influenced the proportion of MNPs in different organs, with faster degradation in the liver regardless of the coating. This information is important for choosing the optimal coating for specific biomedical applications.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Oncology
Vladimir Mulens-Arias, Yadileiny Portilla, Sonia Perez-Yaguee, Raquel Ferreras-Martin, M. Elena Martin, Victor M. Gonzalez, Domingo F. Barber
Summary: Triple-negative breast cancer (TNBC) is a difficult subtype to treat and overactivation of MNK1 has been associated with tumor aggressiveness. This study showed that polyethyleneimine-coated iron oxide nanoparticles (PEI-IONPs) could enhance the intracellular delivery of an MNK1-specific aptamer, resulting in reduced MNK1 signaling and inhibiting TNBC cell migration in vitro. However, the antitumor effect of the aptamer-PEI-IONP complex was compromised in vivo due to minimal accumulation of IONPs in the tumor.
CANCER NANOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
David Egea-Benavente, Carlos Diaz-Ufano, Alvaro Gallo-Cordova, Francisco Javier Palomares, Jhon Lehman Cuya Huaman, Domingo F. Barber, Maria del Puerto Morales, Jeyadevan Balachandran
Summary: This work analyzes the formation mechanism of cubic magnetic iron oxide mesocrystals by thermal decomposition in organic media, finding a nonclassical pathway via the attachment of crystallographically aligned primary cubic particles and sintering to achieve a sizable single crystal. The solvent 1-octadecene and the surfactant agent biphenyl-4-carboxylic acid are key parameters. The degree of aggregation of the cores forming the final particle strongly affects the magnetic properties and hyperthermia efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Yadileiny Portilla, Vladimir Mulens-Arias, Neus Daviu, Alberto Paradela, Sonia Perez-Yague, Domingo F. Barber
Summary: When iron oxide nanoparticles (IONPs) come into contact with biological fluids, they form protein corona complexes that vary in composition based on the origin of the serum. These complexes have an impact on the interaction between IONPs and macrophages, ultimately affecting the immune system.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Immunology
Andres Paris-Munoz, Gonzalo Aizpurua, Domingo F. Barber
Summary: This study revealed the absence of CD8(+) regulatory T cells in two lupus-prone mouse models, MRL/MPJ and MRL/lpr, compared to a non-prone mouse strain, C57BL/6. The findings suggest that Helios plays a regulatory role in the pathogenesis of lupus and its expression profile is altered in other relevant T cell populations.
FRONTIERS IN IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
Andres Paris, Domingo F. Barber
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)