期刊
NATURE METHODS
卷 16, 期 9, 页码 830-842出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-019-0538-0
关键词
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资金
- National Institutes of Health (NIH) Director's New Innovator Award, NIH [EB020892, GM128214, GM132860]
- Alfred P. Sloan Foundation
- Camille and Henry Dreyfus Foundation
- New York Obesity Nutrition Research Center
- Raymond and Beverly Sackler Center Postdoctoral Fellowship
All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.
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