Article
Microbiology
Yiquan Zhang, Yue Qiu, He Gao, Junfang Sun, Xue Li, Miaomiao Zhang, Xingfan Xue, Wenhui Yang, Bin Ni, Lingfei Hu, Zhe Yin, Renfei Lu, Dongsheng Zhou
Summary: The master QS regulator OpaR in Vibrio parahaemolyticus directly regulates a group of genes to impact the concentration of c-di-GMP, which in turn influences behaviors such as biofilm formation. This direct association between QS and c-di-GMP metabolism allows for precise control of gene transcription and bacterial behaviors.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Renfei Lu, Junfang Sun, Yue Qiu, Miaomiao Zhang, Xingfan Xue, Xue Li, Wenhui Yang, Dongsheng Zhou, Lingfei Hu, Yiquan Zhang
Summary: This study revealed how quorum sensing regulates swimming motility in Vibrio parahaemolyticus, showing that its master regulator OpaR directly influences the transcription of Pof genes.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Immunology
Kui Wu, Yongyan Long, Qian Liu, Wei Wang, Guoyin Fan, Hui Long, Yangyun Zheng, Xiansheng Ni, Shengen Chen, Haiying Chen, Shufen Shuai
Summary: This study reveals that the food-borne pathogen Vibrio parahaemolyticus uses quorum sensing signals to regulate the production of its virulence factor T6SS2. CIQS inhibits T6SS2 production through an OpaR-dependent mechanism and also inhibits the adhesion activity of the bacteria to host cells.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Miaomiao Zhang, Xingfan Xue, Xue Li, Qimin Wu, Tingting Zhang, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Renfei Lu, Yiquan Zhang
Summary: Mature biofilm formation by Vibrio parahaemolyticus requires the coordination of multiple factors including exopolysaccharide, type IV pili, and capsular polysaccharide. This coordination is regulated by control pathways such as quorum sensing and c-di-GMP. The QsvR regulator integrates into the quorum sensing regulatory cascade and works together with OpaR to control biofilm formation by regulating the transcription of multiple biofilm-related genes and c-di-GMP metabolism.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Xiangpeng Han, Qingying Chen, Xingguo Zhang, Xiaolan Chen, Dongsheng Luo, Qingping Zhong
Summary: This study found that Lactiplantibacillus plantarum 2057 can effectively inhibit the formation of Vibrio parahaemolyticus biofilm and interfere with its adhesion ability. In addition, the extract of L. plantarum Z057 significantly reduces the biofilm biomass, metabolic activity, and various important components of V. parahaemolyticus. These findings suggest that L. plantarum 2057 could be a biocontrol strategy alternative to chemical methods against foodborne pathogens.
Article
Biochemistry & Molecular Biology
Fuwen Liu, Fei Wang, Yixuan Yuan, Xiaoran Li, Xiaojun Zhong, Menghua Yang
Summary: Vibrio parahaemolyticus is a significant food-borne pathogen. The study showed that the three signal synthases, CqsA(vp), LuxM(vp), and LuxS(vp), are essential for activating quorum sensing and regulating swarming in V. parahaemolyticus. The absence of these synthases leads to swarming defects.
MOLECULAR MICROBIOLOGY
(2023)
Article
Microbiology
Jessica G. Tague, Abish Regmi, Gwendolyn J. Gregory, E. Fidelma Boyd
Summary: Factor for inversion stimulation (Fis) positively regulates the expression of qrr sRNAs in V. parahaemolyticus, controlling swarming motility indirectly through the QS pathway and directly through the surface sensing pathway.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ce Zhang, Min Liu, Ying Wu, Xixi Li, Chen Zhang, Douglas R. Call, Ming Liu, Zhe Zhao
Summary: In Vibrio parahaemolyticus, the expression of T3SS1 is regulated by a complex quorum sensing phosphorelay circuit involving the hybrid histidine kinase ArcB, sensor kinase LuxQ, and response regulator LuxO. The study shows that LuxQ can directly interact with ArcB under high oxygen conditions, leading to phosphorylation of LuxO and activation of T3SS1 expression. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, but ArcA does not participate in regulating T3SS1 expression.
Article
Microbiology
Xiaojun Zhong, Ranran Lu, Fuwen Liu, Jinjie Ye, Junyang Zhao, Fei Wang, Menghua Yang
Summary: This study identified a novel LuxR family regulator, RobA, in Vibrio parahaemolyticus, which influences bacterial growth and biofilm formation, and integrates into the quorum sensing circuit. RobA was found to regulate the expression of exopolysaccharides synthesis cluster and interact with other regulators to construct a complex regulatory network of biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Junfang Sun, Xue Li, Yue Qiu, Xingfan Xue, Miaomiao Zhang, Wenhui Yang, Dongsheng Zhou, Lingfei Hu, Renfei Lu, Yiquan Zhang
Summary: Vibrio parahaemolyticus produces two types of IV pili, MSHA and ChiRP, both of which are required for biofilm formation and pathogen persistence. In this study, it was shown that the master quorum sensing regulators, AphA and OpaR, oppositely regulated the transcription of the pilin encoding gene mshA1. This regulation resulted in a gradual increase in mshA1 expression levels from low to high cell density, demonstrating a mechanism for controlling biofilm formation.
Article
Genetics & Heredity
Long Chen, Miaomiao Zhang, Xue Li, Qimin Wu, Xingfan Xue, Tingting Zhang, Renfei Lu, Yiquan Zhang
Summary: The study elucidated the regulatory mechanism of AphA on the colony morphology variation of Vibrio parahaemolyticus biofilm, which was achieved through its regulation of EPS gene expression.
Article
Food Science & Technology
Wenxiu Zhu, Hongli Liu, Tong Jin, Siyang Chen, Longze Liu, Haisong Wang, Xiaodong Xia
Summary: In this study, a novel gene, VP0143, was identified to positively regulate the biofilm formation of Vibrio parahaemolyticus. Deletion of VP0143 resulted in decreased biofilm formation, weakened flagella-mediated motility and surface hydrophobicity. The study also revealed that VP0143 deletion affected the production of certain biofilm components and led to a loosened biofilm structure.
Article
Biotechnology & Applied Microbiology
Haixi Lu, Yang Sun, Xiaodi Wang, Zhong Lu, Junli Zhu
Summary: This study investigates the inhibitory effects of salinity and citral on the biofilm formation of Vibrio parahaemolyticus (VP) and its potential mechanism. The results show that combining high salinity with citral significantly reduces the biofilm formation of VP and alters the gene expression patterns related to biofilm development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Yanli Xin, Shurui Peng, Shan Wei, Yang Lei, Shuaibing Zhang, Yuansen Hu, Yangyong Lv
Summary: This study investigates the antimicrobial and antibiofilm effects of p-anisaldehyde (PAA) from Pimpinella anisum L. against Vibrio parahaemolyticus, as well as its potential application in food preservation, specifically for salmon.
Article
Microbiology
Yiquan Zhang, Xingfan Xue, Fengjun Sun, Xue Li, Miaomiao Zhang, Qimin Wu, Tingting Zhang, Xi Luo, Renfei Lu
Summary: This study reveals that AphA indirectly activates the transcription of vpa0607-qsvR, while OpaR and QsvR directly repress the transcription of vpa0607-qsvR, resulting in the highest expression levels at low cell density.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jianfeng Wu, Fan Zhang, Guo-Lu Wang, Serhiy Demeshko, Sebastian Dechert, Yi-Quan Zhang, Franc Meyer
Summary: Using a novel tricompartmental hydrazone ligand, a set of trinuclear Dy-3 complexes with different anions (Cl-, Br-, or ClO4-) and co-ligands were isolated and characterized. The magnetic properties of these complexes vary due to structural disorder and different relaxation processes, with Dy-3 center dot ClO4 showing the most robust magnetic behavior. The magneto-structural relationships were analyzed and rationalized using CASSCF/RASSI-SO calculations.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Qian Zou, Guo-Lu Wang, Yi-Qing Chen, Xin-Da Huang, Ge Hua Wen, Ming-Feng Qin, Song-Song Bao, Yi-Quan Zhang, Li-Min Zheng
Summary: This article reports for the first time that X-ray irradiation can break the coordination bond of Dy-OH2 in a metal-organic framework, leading to a structural transformation and significant changes in magnetic properties.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Pan-Dong Mao, Nian-Tao Yao, Hui-Ying Sun, Fei-Fei Yan, Yi-Quan Zhang, Yin-Shan Meng, Tao Liu
Summary: The construction of lanthanide-based molecular magnets using self-assembly strategy is a hot research topic. In this work, the self-assembly reaction of trivalent lanthanide ions, multidentate ligands, and octacyanometallate building blocks was reported. Different types of cyanobridged complexes were obtained and characterized. The magnetic analysis showed that some of these complexes exhibit field-induced single-molecule magnet behaviors.
CRYSTAL GROWTH & DESIGN
(2023)
Article
Chemistry, Multidisciplinary
Hui-Sheng Wang, Chun-Fang Yu, Si-Qi Ye, Yong Chen, Xueru Liu, Yanfang Wu, Pengfei Zhou, Yi-Quan Zhang
Summary: Two novel Co-Dy heterometallic complexes were synthesized using H3L, dpk, and Dy(NO3)3·5H2O reacting with Co(NO3)2·6H2O or anhydrous Co(CH3CO2)2 in CH3OH and CH3CN solutions. Structural analysis revealed that 1 formed a star-like topology with two Dy-III and two Co-III ions, while 2 formed a cross-shaped topology with a linear CoII2Dy(III) paramagnetic unit.
Article
Genetics & Heredity
Long Chen, Miaomiao Zhang, Xue Li, Qimin Wu, Xingfan Xue, Tingting Zhang, Renfei Lu, Yiquan Zhang
Summary: The study elucidated the regulatory mechanism of AphA on the colony morphology variation of Vibrio parahaemolyticus biofilm, which was achieved through its regulation of EPS gene expression.
Article
Chemistry, Inorganic & Nuclear
Yu Jing, Jia Wang, Ming Kong, Gao-Ji Wang, Yi-Quan Zhang, You Song
Summary: Four 3d-4f complexes based on Dy were synthesized, and their magnetic properties were studied. The results showed that all complexes except 4 exhibited single-molecule magnet behavior. The study demonstrated the importance of the coexistence of magnetic anisotropy of lanthanide ions and ferromagnetic coupling between lanthanide and transition metal ions in the design of 3d-4f single-molecule magnets.
INORGANICA CHIMICA ACTA
(2023)
Article
Chemistry, Physical
Hao Sun, Ying Guo, Yanfeng Cui, Dawei Li, Gaocheng Yang, Yeye She, Qian Zhang, Yahong Li, Yi-Quan Zhang, Jinlei Yao
Summary: Two trinuclear complexes, [Dy3(mu 3-H2O)(hmp)3(CH3COO)3] (1) and [Gd3(mu 3-H2O)(hmp)3(CH3COO)3] (2), were synthesized and their crystal structures and magnetic properties were investigated. Complex 1 exhibits field-induced single-molecule magnet behavior, while complex 2 shows weak antiferromagnetic interactions.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Immunology
Miaomiao Zhang, Xingfan Xue, Xue Li, Xi Luo, Qimin Wu, Tingting Zhang, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Renfei Lu, Yiquan Zhang
Summary: This study demonstrated that QsvR negatively regulates the swimming motility of V. parahaemolyticus through direct action on the transcription of Pof genes.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Yiquan Zhang, Xingfan Xue, Fengjun Sun, Xue Li, Miaomiao Zhang, Qimin Wu, Tingting Zhang, Xi Luo, Renfei Lu
Summary: This study reveals that AphA indirectly activates the transcription of vpa0607-qsvR, while OpaR and QsvR directly repress the transcription of vpa0607-qsvR, resulting in the highest expression levels at low cell density.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Yi-Shu Jin, Ke Yu, Nan Zhang, Yi-Quan Zhang, Cai-Ming Liu, Hui-Zhong Kou
Summary: Regulating the magnetic properties through structural transformation and multifunctional magnetic bistable materials attracts increasing interest in the research area of molecular magnets. Herein, we report the synthesis and characterization of a two-dimensional Dy3+ metal-organic framework and three dinuclear Dy-2 complexes. The obtained compounds exhibit excellent thermal stability, ligand-based fluorescence emission, selective adsorption capacity, and typical single-molecule magnetic properties.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Chemistry, Multidisciplinary
Long-He Li, Yan Peng, Shiyong Zhang, Tongtong Xiao, You Song, Heng-Yun Ye, Jinlei Zhang, Yi-Quan Zhang, Zhenxing Wang, Zhao-Bo Hu
Summary: Metal-organic framework (MOF) materials have been synthesized and their magnetic properties studied. The Co(II)-based MOF compound [Co-(dps)2Cl2]n (1) was found to have a two-dimensional (2D) layered structure. The magnetic studies showed that compound 1 exhibited easy-plane anisotropy with positive D value and was a field-induced single-molecule magnet (SMM) governed by single Co(II) ion. The effects of coordinate halogens and packing pattern on the magnetic relaxation were also investigated.
CRYSTAL GROWTH & DESIGN
(2023)
Article
Microbiology
Lei Liu, Wanbing Liu, Yingyu He, Yan Liu, Haisheng Wu, Yiquan Zhang, Qinwen Zhang
Summary: It was found in this study that the global regulator RovM could activate the expression of the hmsB gene, promoting biofilm formation in Yersinia pestis. However, RovM did not bind directly to the upstream DNA region of hmsB, suggesting an indirect regulatory mechanism. These results contribute to the understanding of regulatory circuits controlling gene expression in Y. pestis.
CURRENT MICROBIOLOGY
(2023)
Article
Chemistry, Physical
Tao Shang, Fang Lu, Jin Tao, Yi-Quan Zhang
Summary: Theoretical investigations on mixed-valence dilan-thanide complexes (CpiPr5)2Ln2I3 (Ln = Tb, Dy, and Ho) suggest that the total spin of the 4f shell couples preferentially to the sigma electron spin and then to the orbital angular momentum, enhancing the spin-orbit coupling (SOC) strength. Moreover, the concentration of negative charges in the axial direction leads to a large crystal-field (CF) splitting. Both strong SOC and large CF splitting contribute to the largest energy barrier Ueff observed in such complexes so far. Additionally, the introduction of sigma electrons effectively suppresses the quantum tunneling of magnetization in the ground spin-orbit state, and the Ueff of (CpiPr5)2Ln2I3 is expected to result from the contribution of both Ln ions under strong Ln-sigma exchange coupling.
JOURNAL OF PHYSICAL CHEMISTRY A
(2023)
Article
Microbiology
Miaomiao Zhang, Xingfan Xue, Xue Li, Qimin Wu, Tingting Zhang, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Renfei Lu, Yiquan Zhang
Summary: Mature biofilm formation by Vibrio parahaemolyticus requires the coordination of multiple factors including exopolysaccharide, type IV pili, and capsular polysaccharide. This coordination is regulated by control pathways such as quorum sensing and c-di-GMP. The QsvR regulator integrates into the quorum sensing regulatory cascade and works together with OpaR to control biofilm formation by regulating the transcription of multiple biofilm-related genes and c-di-GMP metabolism.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Yiquan Zhang, Tingting Zhang, Yue Qiu, Miaomiao Zhang, Xiuhui Lu, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Bo Gao, Renfei Lu
Summary: The gene expression profiles during biofilm formation of Vibrio parahaemolyticus were investigated, revealing that multiple genes involved in various cellular pathways were significantly differentially expressed. These pathways include virulence, biofilm formation, metabolism, and regulation.
CURRENT MICROBIOLOGY
(2023)
