Article
Biochemical Research Methods
Hanyuan Zhang, Alanna M. Klose, Benjamin L. Miller
Summary: The study utilized glycan-based receptor analogue microarrays on the AIR platform to achieve label-free, multiplex detection and discrimination of human and avian influenza viruses. The method can be expanded to systematically evaluate influenza virus adaptation in humans, facilitating global influenza surveillance and serving as a new tool enabling quick reactions in the face of a pandemic outbreak for health organizations, governments, research institutes, and laboratories.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Rocco Cancelliere, Alessio Di Tinno, Andrea Maria Di Lellis, Giorgio Contini, Laura Micheli, Emanuela Signori
Summary: This study presents two novel label-free voltammetric immunosensors for rapid and accurate detection of IL-6 in human serum. The immunosensors showed good analytical performances and outperformed a commercial ELISA kit in terms of sensitivity, reproducibility, and detection time. They were also successfully applied in blood samples, demonstrating good sensitivity and reproducibility.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Jairo Pinto de Oliveira, Francisco Burgos-Florez, Isabella Sampaio, Pedro Villalba, Valtencir Zucolotto
Summary: In this study, a label-free impedimetric immunosensor was developed to detect OTA in coffee. The biosensor showed high selectivity and was not affected by matrix interference in coffee samples. It has the advantages of small sample volume, short analysis time, and potential miniaturization.
Article
Nanoscience & Nanotechnology
Mariana Bras, Julia Zanoni, Bruno P. Falcao, Joaquim P. Leitao, Florinda M. Costa, Teresa Monteiro, Sonia O. Pereira, Joana Rodrigues
Summary: Antibiotic pollution is a global concern, so it is important to develop cost-effective and reliable devices for detecting these contaminants. In this study, zinc oxide nanostructures were used as transducers in a fluorescent immunosensor to detect tetracycline. The nanostructures had a high surface area and could interact with antibodies and tetracycline molecules, providing a sensing mechanism. The sensor could quantitatively detect tetracycline in the range of 0.001 to 1 μg/L with a low limit of detection, indicating its advanced performance in this field.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Zhenqiang Ning, Erli Yang, Yongjun Zheng, Mengyuan Chen, Guoqiu Wu, Yuanjian Zhang, Yanfei Shen
Summary: This study proposed a facile synthesis strategy to prepare a self-enhanced ECL emitter DEAMTES@RuSiO2, which exhibited improved ECL properties and film-forming ability critical for ECL biosensors. By combining catalytic hairpin assembly and signal amplification, a label-free ECL biosensor was constructed for highly sensitive detection of miRNA-21 with a detection limit of 8.19 fM, showing superior reliability in detecting miRNA-21 in human serum samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Baha Ondes, Sinem Evli, Murat Uygun, Deniz Aktas Uygun
Summary: A new label-free electrochemical immunosensor modified with boron nitride nanosheets was developed for early detection of CA125 in this study. The immunosensor showed good detection properties and stability, successfully detecting CA125 in artificial serum samples. The prepared immunosensor has potential for point-of-care diagnosis in real cases.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Materials Science, Biomaterials
Kulrisa Kuntamung, Jaroon Jakmunee, Kontad Ounnunkad
Summary: The newly developed electrochemical immunosensor can simultaneously detect three early breast cancer biomarkers with high reliability, low detection limits, and high selectivity, making it suitable for tumor marker detection in human serum.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemical Research Methods
Rosaceleste Zumpano, Manuela Manghisi, Francesca Polli, Cristine D'Agostino, Federica Ietto, Gabriele Favero, Franco Mazzei
Summary: This study developed a label-free electrochemical immunosensor for the quick, cheap, and straightforward determination of atrazine, achieving detection through a technological platform and specific treatment methods. Under optimal experimental conditions, the immunosensor exhibited good linearity range, limit of detection, reproducibility, and stability, making it suitable for successful application in the detection and monitoring of atrazine in real water samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yesol Moon, Sojeong Lee, Jinyoung Kim, Geunseon Park, Chaewon Park, Jong-Woo Lim, Minjoo Yeom, Daesub Song, Seungjoo Haam
Summary: This study presents a new method (PVFIA) for detecting influenza A virus. The method uses a fusion assay with gold nanoparticle encapsulating fusogenic vesicles to capture the virus and achieve visual detection through color change. The results show a low detection limit and high sensitivity, indicating the effectiveness of the method for virus detection.
Article
Engineering, Environmental
Peng Wang, Gege Yu, Juan Wei, Xingrui Liao, Yao Zhang, Yarong Ren, Cui Zhang, Yueqi Wang, Daohong Zhang, Jianlong Wang, Yanru Wang
Summary: This study presents a label-free immunostaining strategy based on bacteriophage-displayed nanobody for the detection of Vibrio parahaemolyticus. The developed assay allows simple and sensitive detection of the pathogen within a short period of time, with high specificity and no cross-reactivity towards other bacterial species.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Chemistry, Analytical
Dagang Jiang, Yafei Tian, Yujiao Zhang, Xueyun Lu, Dan Xiao, Cuisong Zhou
Summary: A rapid and low-cost label-free imaging array for multiplexed detection of avian influenza virus DNA biomarkers has been developed. It can simultaneously detect three subtypes of AIV DNA biomarkers quickly within 20 minutes, with a small reagent volume and a smartphone. With advantages of fast detection, low cost, and multiplexed analysis, the imaging array has potential applications for early infectious disease diagnosis in resource-constrained settings.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Shereena Joseph, Soumya Rajpal, Debashree Kar, Shital Devinder, Saurabh Pandey, Prashant Mishra, Joby Joseph
Summary: In this study, an optical detection scheme based on guided mode resonance (GMR) was developed for the identification of a specific bacteria. The sensitivity of the sensor was evaluated and a good detection limit was achieved. The developed GMR biosensor is low-cost, easy to handle, and can be transformed into a portable detection modality.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Jantima Upan, Napaporn Youngvises, Adisorn Tuantranont, Chanpen Karuwan, Philippe Banet, Pierre-Henri Aubert, Jaroon Jakmunee
Summary: A label-free electrochemical aptamer-based sensor was developed for AFP detection, utilizing a PtNPs/GO-COOH modified platform with an AFP aptamer as the recognition element. The sensor demonstrated good sensitivity, selectivity, and stability for sensitive detection of AFP in serum samples.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Madisson Chabas, Olivier Pible, Jean Armengaud, Beatrice Alpha-Bazin
Summary: To accurately and rapidly identify microorganisms from complex microbiota for clinical diagnostic purposes, an innovative label-free multiplexing method for the identification of microorganisms using tandem mass spectrometry is proposed in this paper. The method involves offline reversed-phase fractionation of individual peptidomes and achieves multiplexing by mixing fractions of staged hydrophobicity. This approach enables the identification of up to 21 microorganisms in a single 60-minute run without the use of labeling reagents, providing new perspectives for high-throughput proteotyping of bacteria in large culturomics projects.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Hanyuan Zhang, Alanna M. Klose, Benjamin L. Miller
Summary: The study utilized glycan-based receptor analogue microarrays on the AIR platform to achieve label-free, multiplex detection and discrimination of human and avian influenza viruses. The method can be expanded to systematically evaluate influenza virus adaptation in humans, facilitating global influenza surveillance and serving as a new tool enabling quick reactions in the face of a pandemic outbreak for health organizations, governments, research institutes, and laboratories.
BIOCONJUGATE CHEMISTRY
(2021)
Review
Biochemical Research Methods
Qinglan Huang, Nantao Li, Hanyuan Zhang, Congnyu Che, Fu Sun, Yanyu Xiong, Taylor D. Canady, Brian T. Cunningham
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)