Article
Agriculture, Dairy & Animal Science
Lu Zhou, Jinhong Lei, Xiaofei Zhai, Ningning Lu, Haitao Shi, Jichao Wang
Summary: Vocalization is crucial for the survival of underwater animals. The Chinese soft-shelled turtle, a highly vulnerable species, has not been studied for its underwater vocalization, which can provide important insights for ecological and evolutionary research. By conducting underwater recordings, we identified 10 call types of the turtle and observed differences in vocalizations between individuals of different ages and sexes. The Chinese soft-shelled turtle has a diverse range of vocalizations and shows the tendency for vocalization to become more diverse with age, aiding its adaptation to complex and dim underwater environments.
Article
Fisheries
Peng Zhang, Naicheng Liu, Zidong Xiao, Mengjie Zhang, Yan Meng, Yuding Fan, Xiaowei Hu, Junqiang Qiu, Qinghua Zhang, Yong Zhou
Summary: White abdominal disease is a major disease in Chinese soft-shelled turtle aquaculture. Pathological characteristics of diseased turtles were studied and showed changes in hematological, histopathological, intestinal microbial, and immune gene expression. The diseased turtles exhibited anemia, visceral tissue lesions, reduction of intestinal microbial species, and innate immune activation.
AQUACULTURE REPORTS
(2023)
Article
Chemistry, Applied
Fen Wang, Zhu Chen, Yunsheng Cheng, Guanjun Hou, Suofei Ji, Ye Zhang, Jie Li, Chengjun Zhu, Yuchun Wu, Guangtong Song, Yelin Jiang
Summary: The study found that the muscle of Yangtze and Japanese strains of Chinese soft-shelled turtle is high in protein and low in fat, making it beneficial for human health, but caution should be exercised when consuming the calipash.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Fisheries
Yuqi Mu, Mengyan Lan, Yali Li, Zhao Zhang, Yueqiang Guan
Summary: Florfenicol has significant effects on the antioxidant and immune systems of the Chinese soft-shelled turtle (Pelodiscus sinensis). Various dosage levels of florfenicol were found to influence antioxidant enzyme activities and immune-related gene expression. The study provides insights into the molecular mechanisms and pathways involved in the florfenicol response of P. sinensis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Pei Wang, Gang Xiong, Dan Zeng, Jianguo Zhang, Lingrui Ge, Li Liu, Xiaoqing Wang, Yazhou Hu
Summary: MicroRNAs (miRNAs) play important regulatory roles in pigmentation processes in aquatic animals. In this study, we performed an integrated analysis of miRNA and mRNA expression profiles in the skin of Chinese soft-shelled turtles during three embryonic stages. We identified novel genes and miRNAs, and found differentially expressed genes (DEGs) and miRNAs (DEMs) between different stages. Some of these DEGs and DEMs may be associated with turtle pigmentation in embryonic development. Our findings provide insights into the regulatory patterns of miRNAs in reptile pigmentation and may contribute to understanding the molecular processes involved in pigmentation.
Article
Marine & Freshwater Biology
Rui Feng, Zhao Zhang, Yueqiang Guan
Summary: Nitrite affects the oxygen-carrying capacity of blood, liver antioxidant system, and lipid peroxidation in Chinese soft-shelled turtles, while also triggering immune responses. The study identified differentially expressed genes and pathways involved in cellular responses to nitrite stress.
AQUATIC TOXICOLOGY
(2021)
Article
Environmental Sciences
Yufei Huang, Sheng Yang, Xuebing Bai, Yonghong Shi, Qiusheng Chen
Summary: The Chinese soft-shelled turtle is one of the most primitive amphibians and reptiles, attracting attention due to its environmental suitability and unique hibernation habits. Research on the molecular mechanisms underlying the periodic variation of lipid droplets in the liver of this species revealed a shift in energy metabolism during hibernation, with lipid oxidation becoming the main mode of energy generation. Further analysis showed that lipolysis plays a key role in the degradation of hepatic lipid droplets during hibernation in the Chinese soft-shelled turtle.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Reproductive Biology
Bangsai Yang, Wei Sun, Lin Jin, Pan Li, Yingjie Zhou, Guoying Qian, Chutian Ge, Ling Xiao
Summary: SOX8 is found to be a key regulator of early male differentiation in Chinese soft-shelled turtles, as it exhibits male-specific higher expression during the critical period of sex determination and is rapidly down-regulated during male-to-female sex reversal. Knockdown of SOX8 leads to complete feminization, highlighting its crucial role in reptile sex determination.
BIOLOGY OF REPRODUCTION
(2023)
Article
Environmental Sciences
Jing Xiang, Hao Wu, Jinwei Gao, Weimin Jiang, Xing Tian, Zhonggui Xie, Tao Zhang, Jia Feng, Rui Song
Summary: Niclosamide (NIC) is a molluscicide used for controlling schistosomiasis, but its negative effects on aquatic animals, such as Chinese soft-shelled turtles, have been reported. This study investigated the toxicity mechanism of NIC on Chinese soft-shelled turtles and found that NIC exposure induced severe liver damage and increased ROS production. The activities of antioxidant enzymes were inhibited, indicating a disruption in the antioxidant defense. Transcriptome sequencing identified the disorder of lipid metabolism and innate immune regulation as toxicological properties of NIC. Overall, this study provides valuable information on the toxic effect of NIC on Chinese soft-shelled turtles.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Fisheries
Junxian Zhu, Luo Lei, Chen Chen, Jiansong Li, Yongchang Wang, Congcong Wu, Yakun Wang, Xiaoyou Hong, Xiaoli Liu, Lingyun Yu, Haigang Chen, Chengqing Wei, Yihui Liu, Wei Li, Xinping Zhu
Summary: In this study, a medium-free incubation method for hatching Pelodiscus sinensis eggs was developed and compared with the traditional vermiculite incubation method. The results showed that medium-free incubation did not significantly affect hatching success, body weight, carapace length, carapace width, plastron length, plastron width, and righting response time of P. sinensis compared to the traditional vermiculite incubation. Furthermore, light treatment shortened the incubation period and had negative effects on certain parameters during medium-free incubation. This study also discovered the phenomenon of egg-hatching synchrony in P. sinensis.
AQUACULTURE REPORTS
(2023)
Article
Materials Science, Multidisciplinary
Yufei Huang, Xiaoya Chu, Yafei Zhang, Sheng Yang, Yonghong Shi, Qiusheng Chen
Summary: This study aimed to investigate the alteration of mitochondrial morphology and its potential role in the Chinese soft-shelled turtle during different stages of hibernation. The results showed that the mitochondrial architecture changed from round to crescent during hibernation, and there was an enhanced interaction between lipid droplets and mitochondria. Additionally, mitochondrial fusion, fission, and mitophagy were accelerated in different stages of hibernation, and the gene and protein expression associated with these processes correlated with the mitochondrial ultrastructural changes.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Genetics & Heredity
Tong Zhou, Hang Sha, Meng Chen, Guobin Chen, Guiwei Zou, Hongwei Liang
Summary: This study revealed that the Chinese soft-shelled turtle exhibits sex dimorphism, with exogenous hormones being able to induce the transformation from male to female phenotype. Small RNA-seq analysis showed significant differences in miRNA expression between males, females, and pseudo-females, with certain miRNAs playing a crucial role in gonadal development and the sexual reversal process. The findings suggest that these miRNAs may contribute to the breeding of all-male strains of Chinese soft-shelled turtles.
Article
Fisheries
Xianwen Zhou, Yu Tian, Li Liu, Lingrui Ge, Hui Zhang, Yixin Liang, Xiaoqing Wang, Yazhou Hu
Summary: This study reported a case of tapeworm infection in Chinese soft-shelled turtle and investigated its taxonomic identification and effects on the turtle. The tapeworm infection caused nutrition loss, liver and intestinal lesions, and altered nutrient metabolism in the turtle. Furthermore, the tapeworm infection affected the immune responses of the turtle and increased the abundance of certain pathogenic bacteria in the gut microflora.
Article
Immunology
Lingrui Ge, Yi Yu, Xingxing Wen, Hewei Xiao, Kejun Liu, Zhuying Liu, Shuai Liu, Qian Li, Xiaoqing Wang, Zaofu Deng, Yazhou Hu
Summary: This study investigated the effects of dietary sodium butyrate (SB) on the growth performance, immune function, and intestinal microflora of Chinese soft-shelled turtles. The results showed that the addition of 0.02% SB to the diet improved the growth rate, immune function, feed conversion ratio, and total cholesterol content of the turtles. Furthermore, the abundance of specific microbial species was increased with 0.02% SB supplementation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Fisheries
Xiao Ma, Guiyu Wang, Huifen Liu, Limin Wu, Xue Tian, Luming Wang, Shuangshuang Cen, Chao Zhang, Junchang Feng, Qisheng Wu, Xiaoqing Wang, Xuejun Li
Summary: Aromatase, encoded by Cyp19a1, plays a crucial role in estrogen biosynthesis and is involved in sex differentiation and ovary development. This study demonstrated that aromatase may have functional significance in spermatogenesis in Chinese soft-shelled turtles, as indicated by changes in gene expression patterns following letrozole treatment.
Article
Biochemistry & Molecular Biology
Yiwen Zhong, Yaxiong Pan, Li Liu, Honghui Li, Yulong Li, Jian Jiang, Jing Xiang, Jianshe Zhang, Wuying Chu
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ping Wu, Jia Cheng, Lin Chen, Jing Xiang, Yaxiong Pan, Yongzhan Zhang, Tao Zheng, Naiyuan Liu, Wuying Chu, Jianshe Zhang
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Ping Wu, Aimin Wang, Jia Cheng, Lin Chen, Yaxiong Pan, Honghui Li, Qi Zhang, Jiaqi Zhang, Wuying Chu, Jianshe Zhang
MARINE BIOTECHNOLOGY
(2020)
Article
Genetics & Heredity
Honghui Li, Yaxiong Pan, Lingsheng Bao, Yulong Li, Congyi Cheng, Li Liu, Jing Xiang, Jia Cheng, Jianshe Zhang, Wuying Chu, Yudong Shen
Article
Multidisciplinary Sciences
Hong-Hui Li, Ling-Sheng Bao, Shi-Ming Deng, Li Liu, Jia Cheng, Xiao Chen, Ya-Xiong Pan, Jian-She Zhang, Wu-Ying Chu
Summary: The muscle nutritional composition and muscle fiber structure were notably influenced by pathogen infection, resulting in increased oxidative stress and autophagy. However, N-acetylcysteine treatment may mitigate these effects by decreasing ROS level and regulating antioxidant signaling pathways.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Xin Zhu, Jiangli Hu, Jianshe Zhang, Jingjie Liu, Lingsheng Bao, Yaxiong Pan, Jia Cheng, Wuying Chu
Summary: The study revealed that the catabolism of BCAAs in the skeletal muscle of Chinese perch was enhanced to maintain physiological homeostasis during food deprivation and glucocorticoid stress. KLF15 plays a crucial role in the adaptive regulation of BCAAs metabolism in Chinese perch skeletal muscle.
AQUACULTURE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ping Wu, Lin Chen, Jia Cheng, Yaxiong Pan, Xin Zhu, Lingsheng Bao, Wuying Chu, Jianshe Zhang
Summary: Fish skeletal muscles are composed of slow and fast muscles, which have differences in carbohydrate and lipid metabolism. Analysis of miRNAs revealed that more miRNAs inhibit the expression of target genes in slow muscles, which may contribute to the differences in glucose and lipid metabolism between muscles.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Fisheries
Yaxiong Pan, Jinsheng Tao, Jun Zhou, Jia Cheng, Yuanhua Chen, Jing Xiang, Lingsheng Bao, Xin Zhu, Jianshe Zhang, Wuying Chu
Summary: Starvation can induce oxidative stress, disrupt antioxidant systems, and cause autophagy and mitochondrial dysfunction in the intestine of Chinese perch.
Article
Biochemistry & Molecular Biology
Yaxiong Pan, Lin Chen, Jia Cheng, Xin Zhu, Ping Wu, Lingsheng Bao, Wuying Chu, Shan He, Xufang Liang, Jianshe Zhang
Summary: Fish skeletal muscles consist of spatially well-separated fiber types, namely red and white muscles with different physiological functions and metabolism. DNA methylation profiles differ between the two types of muscle tissues, affecting muscle growth and development.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Fisheries
Ping Wu, Lin Chen, Jia Cheng, Yaxiong Pan, Xin Zhu, Wuying Chu, Jianshe Zhang
Summary: This study investigated the effects of starvation and diet after starvation on the autophagy of adult Chinese perch muscle. The results showed that after 3 days of starvation, the autophagy marker protein LC3 and the number of autophagosomes remained unchanged. However, after 7 days of starvation, the number of autophagosomes increased along with a decrease in antioxidant molecules and the accumulation of ROS. Refeeding after starvation effectively reduced ROS levels, restored antioxidant enzyme activity, decreased autophagy, and improved feed efficiency.
JOURNAL OF FISH BIOLOGY
(2022)
Article
Nutrition & Dietetics
Xin Zhu, Jingjie Liu, Minglang Cai, Lingsheng Bao, Yaxiong Pan, Ping Wu, Wuying Chu, Jianshe Zhang
Summary: This study reveals that short-term fasting significantly affects the expression of 15 core clock genes, as well as KLF15 and Bcat2, in the fast muscle of Siniperca chuatsi. The correlation between Clock and KLF15 expression is enhanced after fasting treatment. Moreover, it is demonstrated that the transcriptional expression of KLF15 is regulated by Clock, and the transcriptional expression of Bcat2 is regulated by KLF15. These findings highlight the interaction between circadian rhythm and BCAA metabolism, and suggest that fasting induces a switch in KLF15 expression through affecting the rhythmic expression of Clock, which in turn promotes the metabolism of BCAA to maintain energy homoeostasis.
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Fisheries
Yu Zhang, Jia Cheng, Junzhao Wang, Junxin Ma, Jialin Pan, Xinyi Ma, Linyi He, Xin Zhu, Lingsheng Bao, Wuying Chu, Jianshe Zhang, Yaxiong Pan
Summary: Fatty acid-binding proteins (FABPs) act as fatty acid chaperones and play a crucial role in lipid metabolism. This study identified 13 fabp genes in Chinese perch, investigating their spatial and temporal expression profiles, circadian rhythm in the intestine, and transcriptional regulation under cadmium exposure. Phylogenetic analysis revealed that a teleost-specific genome duplication event contributed to the expansion of Sc-fabp genes.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Jia Cheng, Xin Zhu, Yangyang Meng, Congyi Cheng, Wei Zeng, Lin Chen, Yaxiong Pan, Wuying Chu, Jianshe Zhang
Summary: In this study, the expression patterns of Tet1, Tet2, and Tet3 genes in Chinese perch were investigated. The results showed differential expression of these genes at different developmental stages and in different tissues. The study revealed that Tet1/2 may target MyoD and MRF4, promoting their expression and stimulating myoblast differentiation.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Yu Zhang, Yaxiong Pan, Jinsheng Tao, Jun Zhou, Jialin Pan, Ping Wu, Wenping Yang, Hongyan Tian, Wuxiao Zhang, Zhiqiang Xu, Jianshe Zhang, Wuying Chu, Aimin Wang
Summary: This study investigated the effects of acute cadmium exposure on lipid metabolism disorder, oxidative stress, and autophagy in the abdominal muscle of Procambarus clarkii. The results showed that acute cadmium exposure induced lipid metabolism disorder and oxidative stress, and inhibited the expression of autophagy-related genes. These findings contribute to understanding the mechanism of cadmium muscular toxicity in crustaceans.
AQUACULTURE REPORTS
(2023)
Article
Environmental Sciences
Zhenyan Dai, Jia Cheng, Lingsheng Bao, Xin Zhu, Honghui Li, Xiao Chen, Yu Zhang, Jianshe Zhang, Wuying Chu, Yaxiong Pan, Huang Huang
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.
