Article
Nanoscience & Nanotechnology
Bong-Rim Shin, In Cheol Yu, Myeong-Hoon Shin, Mojca Jazbinsek, Fabian Rotermund, O-Pil Kwon
Summary: In this study, efficient organic THz wave generators, 2-(4-hydroxystyryl)-1-methylquinolinium 4-bromobenzenesulfonate (OHQ-BBS) single crystals, were reported. The OHQ-BBS crystals exhibit a wide molecular vibrational mode-free range in the THz frequency region with low absorption. By optical rectification, the OHQ-BBS crystals generate extremely broad, dimple-free THz waves and achieve a higher THz electric field compared to the widely used ZnTe inorganic crystal.
Review
Chemistry, Analytical
Siying Xie, Zhirun Ji, Tiying Suo, Bingzhi Li, Xing Zhang
Summary: CRISPR/Cas technology, originating from bacteria's adaptive immune system, has been recognized by the Nobel Prize in Chemistry for its achievements in gene editing. It has a wide range of applications, including biosensors detection, with advantages such as high accuracy, specificity, rapid response, and integrity, attracting significant research interest. In addition to nucleic acid monitoring, CRISPR technology also shows promise in detecting various biomarkers, making it valuable to explore and summarize strategies for recognizing a broad range of targets.
ANALYTICA CHIMICA ACTA
(2021)
Article
Nanoscience & Nanotechnology
Jilai Jia, Xiaoping Du, Xuqin Lv, Fang Xie, Jiaojiao Zhou, Jie Cai
Summary: A DNA-mediated growth strategy was developed in this study for the synthesis of platinum nanoparticles (PtNPs) on the surface of graphene oxide (GO). The resulting GO-DNA-PtNPs exhibited excellent peroxidase-mimicking activity, and the activity could be inhibited by Hg(2+) due to the specific trapping of Hg2+ by thymine bases (T-Hg2+-T) and in situ reduction of Hg2+ by PtNPs on the GO surface. The sensor showed good linear range (from 1 pM to 1 μM) and a detection limit of 2.2 pM for Hg2+ analysis, demonstrating excellent analytical performance in complex lake waters.
ACS APPLIED NANO MATERIALS
(2023)
Article
Agriculture, Multidisciplinary
Xiaoze Dong, Qianqian Cheng, Shuo Qi, Mingwei Qin, Ning Ding, Yuhan Sun, Yu Xia, Yin Zhang, Zhouping Wang
Summary: We present a method using a riboswitch sensor to detect tetracycline content in foods. The sensor is based on a lyophilized cell-free expression system, allowing for long-term storage. The riboswitch, constructed with artificially screened tetracycline RNA aptamers, can be used to detect tetracyclines in various samples, with a detection limit as low as 0.47 μM. Additionally, this method enables qualitative detection of tetracyclines in milk samples by the naked eye.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
S. V. Vamsi Bharadwaj, Diptee S. Tiwari, Tonmoy Ghosh, Sandhya Mishra
Summary: This study describes the construction of an improved RSF1010-based vector and a temperature-inducible RNA thermometer. The vector, named pSM201v, can be used as an expression vector in both Gram positive and a wide range of Gram negative bacteria, including cyanobacteria. The RNA thermometer, named DTT1, allows precise control of gene expression through temperature induction.
Review
Biochemistry & Molecular Biology
Izabela Kern-Zdanowicz
Summary: pCTX-M3 is the archetype of IncM incompatibility group of conjugative plasmids responsible for worldwide dissemination of antibiotic resistance genes and has a limited host range within Enterobacteriaceae, while its conjugation system has a wide range of recipients including Alpha-, Beta-, and Gammaproteobacteria, and Firmicutes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Environmental
Cornelia Kienle, Inge Werner, Stephan Fischer, Christina Luthi, Andrea Schifferli, Harrie Besselink, Miriam Langer, Christa S. McArdell, Etienne L. M. Vermeirssen
Summary: Ozonation can effectively eliminate micropollutants in wastewater, but it may also produce reactive and potentially toxic transformation products. Additional post-treatments are recommended to further remove these products. The study found that ozonation reduced ecotoxicological effects, and post-treatments further reduced these effects. Rainbow trout liver gene expression and algal photosynthesis were sensitive indicators for evaluating the treatment efficiency.
Article
Infectious Diseases
Maria Getino, Maria Lopez-Diaz, Nicholas Ellaby, John Clark, Matthew J. Ellington, Roberto M. La Ragione
Summary: This study evaluated the transmission potential of plasmids encoding the emergent NDM-1 carbapenemase compared to the pandemic OXA-48. The results showed that these plasmids can successfully transfer between different bacterial species, with different burdens. In particular, the IncM2 plasmids carrying NDM-1 were considered emergent threats that need continuous monitoring.
Article
Chemistry, Analytical
Chen-chen Li, Yue-hong Dong, Xiaoran Zou, Xiliang Luo, Dazhong Shen, Juan Hu, Chun-yang Zhang
Summary: A label-free and template-free chemiluminescent biosensor, termed hmC-GLIB-IAS, has been developed for sensitive detection of 5hmC in genomic DNA. This method shows advantages of bisulfite-free, improved sensitivity, and genome-wide analysis of 5hmC at constant reaction temperature. It can detect 5hmC with a high reproducibility and accuracy without isotope-labeled substrates or specific antibodies.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Bingyang Huo, Yuling Hu, Zhixian Gao, Gongke Li
Summary: This article reviewed the application of functional nucleic acid-based biosensors in food safety analysis, including their programmability, structural switches, and detection methods for various food contaminants.
Article
Biotechnology & Applied Microbiology
Sydney A. Smith, Caleb M. Lindgren, Landon E. Ebbert, Tyler J. Free, J. Andrew D. Nelson, Katelyn M. Simonson, J. Porter Hunt, Bradley C. Bundy
Summary: Cell-free protein synthesis (CFPS) is a versatile biotechnology platform with applications in clinical diagnostics, therapeutics production, and research. However, CFPS systems are time-consuming and expensive. This work presents a new CFPS system that reduces reagent cost and preparation time by eliminating the need for separately purified DNA templates. This system shows potential for cost reduction and use in low-resource settings.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Chemistry, Analytical
Abdullah Al Noman, Jitendra Narayan Dash, Md Abdullah Al Maruf, Cheng Xin, Hwa-Yam Tam, Changyuan Yu
Summary: A label-free-based fiber optic biosensor based on etched tilted Bragg fiber grating (TFBG) is proposed and practically demonstrated. The hybridization of probe DNA with the complementary DNA (cDNA) has been monitored using wavelength-dependent interrogation. The reproducibility of the probes has been demonstrated and the response towards non-cDNA has also been investigated to establish its specificity.
Article
Engineering, Environmental
Shudi Liu, Xia Zhang, Chaoxian Yan, Panpan Zhou, Li Zhang, Qingzhong Li, Renjie Zhang, Lingxin Chen, Liangwei Zhang
Summary: The development of new fluorescent probes for mercury ion analysis is rapidly growing due to the detrimental toxicity of mercury to environmental safety, ecological security, and human health. The synthesized Naph-NSS small molecule probe shows high sensitivity and selectivity towards mercury ions at low pH, and can be loaded on paper strips for instant detection.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Goeun Park, Hanbin Park, Sang-Chan Park, Moonbong Jang, Jinho Yoon, Jae-Hyuk Ahn, Taek Lee
Summary: Zika virus (ZIKV) and dengue virus (DENV) infections are becoming more common due to global warming. Accurate diagnosis is essential for controlling these viral infections. Recent research has focused on developing biosensors for the accurate diagnosis of ZIKV and DENV using DNA nanotechnology. These biosensors offer advantages over traditional diagnostic methods, such as PCR and ELISA. DNA can serve as a bioreceptor and be easily modified with functional groups, making it a valuable tool for the development of specific bioreceptors that bind to viral proteins or nucleic acids. This review discusses advances in DNA-nanotechnology-based biosensors for the detection of ZIKV and DENV.
Article
Biochemistry & Molecular Biology
Nur Diyana Jamaluddin, Nur-Fadhilah Mazlan, Ling Ling Tan, Nurul Yuziana Mohd Yusof, Bahariah Khalid
Summary: In this study, an acrylic micropolymer-based RNA biosensor was developed to detect viral RNA using a G-quadruplex DNA probe. The biosensor showed a rapid response time and high sensitivity, allowing real-time detection of DENV2 in non-invasive human fluid samples.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Analytical
Alexandros Katranidis, Joerg Fitter
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Physical
Niklas O. Junker, Farzaneh Vaghefikia, Alyazan Albarghash, Henning Hoefig, Daryan Kempe, Julia Walter, Julia Otten, Martina Pohl, Alexandros Katranidis, Simone Wiegand, Joerg Fitter
JOURNAL OF PHYSICAL CHEMISTRY B
(2019)
Article
Chemistry, Analytical
Xiaoming Fan, Johnny Hendriks, Maddalena Comini, Alexandros Katranidis, Georg Bueldt, Thomas Gensch
METHODS AND APPLICATIONS IN FLUORESCENCE
(2020)
Article
Biology
Florian Wruck, Pengfei Tian, Renuka Kudva, Robert B. Best, Gunnar von Heijne, Sander J. Tans, Alexandros Katranidis
Summary: The study by Wruck et al. investigates the folding of the small zinc-finger domain ADR1a inside and at the vestibule of the ribosomal tunnel, using optical tweezers, single-molecule FRET, and molecular dynamics simulations. They find that the ribosomal tunnel accelerates folding while stabilizing the folded state like chaperonins. This study provides insights into the role of the ribosomal tunnel in the folding dynamics of nascent polypeptides.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Physical
Fani Tsitouroudi, Vasiliki Sarli, Dimitrios Poulcharidis, Maria Pitou, Alexandros Katranidis, Theodora Choli-Papadopoulou
Summary: Reversine is a small organic molecule that induces dedifferentiation of myoblasts and selectively leads cancer cells to cell death. Aza-Reversine and its biotinylated derivative can reprogram cells and promote osteoblast differentiation.
Article
Cell Biology
Andrey Bogorodskiy, Ivan Okhrimenko, Ivan Maslov, Nina Maliar, Dmitrii Burkatovskii, Florian von Ameln, Alexey Schulga, Philipp Jakobs, Joachim Altschmied, Judith Haendeler, Alexandros Katranidis, Ivan Sorokin, Alexey Mishin, Valentin Gordeliy, Georg Buldt, Wolfgang Voos, Thomas Gensch, Valentin Borshchevskiy
Summary: The study shows that a new microinjection method can be used to directly observe protein transport in living cells. The researchers found that releasing nascent polypeptides from translating ribosomes with puromycin increased the import rate of proteins into mitochondria.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Physical
Abhishek Kumar Pandey, Niyati Piplani, Titas Mondal, Alexandros Katranidis, Jaydeep Bhattacharya
Summary: With the advancement of drug discovery, nano-sized polymer and lipid-based drug delivery systems have gained increasing interest due to their enhanced distribution, bioavailability, and therapeutic value. Membrane scaffold protein embedded POPC nanodiscs have been investigated as efficient hydrophobic drug delivery vehicles, showing sustained drug release and significant enhancement of cell death and toxicity in vitro and in 3D spheroid models compared to free drug.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Olessya Yukhnovets, Henning Hofig, Nuno Bustorff, Alexandros Katranidis, Jorg Fitter
Summary: For single-molecule studies in solution, very low concentrations of dye-labelled molecules are needed to achieve single-molecule sensitivity. Specific demands on surface passivation are required to ensure stability of molecule concentration over a period of several hours. Diffusion rate and absolute molecule concentrations are important for controlling the number of molecules in the detection volume.
Article
Engineering, Biomedical
Stefanos Pendas, Antonis Asiminas, Alexandros Katranidis, Costas Tsioptsias, Maria Pitou, Georgios Papadopoulos, Theodora Choli-Papadopoulou
Summary: This study successfully constructed biofunctionalized non-adherent surfaces by immobilizing the domain D of protein A from Staphylococcus aureus onto cellulose acetate scaffolds using streptavidin/biotin interaction. The interaction between protein A and von Willebrand factor A1 effectively inhibited the growth of S. aureus. These scaffolds have the potential for important medical applications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Meeting Abstract
Biophysics
Nuno Bustorff Silva, Alexandros Katranidis, Joerg Fitter
BIOPHYSICAL JOURNAL
(2022)
Article
Biology
Henning Hoefig, Olessya Yukhnovets, Cristina Remes, Noemie Kempf, Alexandros Katranidis, Daryan Kempe, Joerg Fitter
COMMUNICATIONS BIOLOGY
(2019)
Meeting Abstract
Biophysics
F. Wruck, R. Kudva, S. J. Tans, G. Von Heijne, A. Katranidis
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2019)
Article
Biology
Mayuri Sadoine, Michele Cerminara, Joerg Fitter, Alexandros Katranidis