Review
Biochemistry & Molecular Biology
Pooja Bhardwaj, Rajni Kant, Sthita Pragnya Behera, Gaurav Raj Dwivedi, Rajeev Singh
Summary: This article emphasizes the importance of CRISPR/Cas technology as a diagnostic tool for early management, diagnosis, and treatment of infections and diseases, and explores its detection capabilities for non-nucleic acid targets as well as the technical challenges and improvements in clinical assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Fan Li, Qinghua Ye, Moutong Chen, Baoqing Zhou, Jumei Zhang, Rui Pang, Liang Xue, Juan Wang, Haiyan Zeng, Shi Wu, Youxiong Zhang, Yu Ding, Qingping Wu
Summary: The study introduces the trans-cleavage activity of CRISPR/Cas12a into an electrochemical biosensor (ECRISPR) combined with recombinase-assisted amplification (RAA) to develop a cost-effective, specific, and ultrasensitive method for Listeria monocytogenes detection. The RAA-based E-CRISPR sensor can detect as low as 0.68 aM of genomic DNA and 26 cfu/mL of L. monocytogenes in pure cultures, demonstrating rapid and ultrasensitive detection capabilities in spiked and natural samples. This system shows no cross-reactivity with other non-target bacteria, making it a simple, highly sensitive, and accurate platform for L. monocytogenes detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jianhao Xu, Jianfeng Ma, Yanwei Li, Lin Kang, Bing Yuan, Shiqing Li, Jie Chao, Lianhui Wang, Jinglin Wang, Shao Su, Yuan Yuan
Summary: Brucellosis, caused by Brucella spp., is a disease that leads to long-term damage in humans and livestock. Researchers have developed a dual biosensor based on CRISPR/Cas12a, which can rapidly and accurately detect various Brucella strains and distinguish them from non-target bacteria.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Electrochemistry
Xiwei Zhuang, Xueqiong Yang, Bo Cao, Haiming Sun, Xiaoyan Lv, Chijia Zeng, Fugang Li, Bin Qu, H. Susan Zhou, Feiyun Cui, Qin Zhou
Summary: The CRISPR/Cas system has gained attention for its gene-editing capabilities. The reported trans-cleavage activity of Cas proteins has increased the system's diagnostic ability. When applied in electrochemical biosensors, the CRISPR/Cas system provides high specificity, low detection limits, and meets point-of-care testing requirements. This review introduces the history, advantages, and applications of EC biosensors based on the CRISPR/Cas system, and proposes future prospects.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Review
Chemistry, Physical
Weiran Su, Junru Li, Chen Ji, Congshuo Chen, Yuzheng Wang, Huili Dai, Fengqin Li, Peifeng Liu
Summary: CRISPR/Cas systems are powerful tools for disease biomarkers detection, capable of detecting nucleic acid and non-nucleic acid targets. This review summarizes the principles and characteristics of various CRISPR/Cas systems, and introduces the applications of CRISPR/Cas systems in detecting nucleic and non-nucleic acid targets. The prospects and challenges of their applications in biosensing are also discussed.
Article
Nanoscience & Nanotechnology
Qiong Hu, Yilin Luo, Xiaojing Cao, Zhuohua Chen, Yanyu Huang, Li Niu
Summary: A method for highly selective and amplified sensing of ultralow-abundance nucleic acids using PNA recognition probe and BERP-based amplification strategy is presented. The method is cost-effective, easy to operate, highly selective, and has a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Jinying Dong, Xiaoya Wu, Qiushi Hu, Chongsi Sun, Jiahao Li, Peng Song, Yan Su, Lei Zhou
Summary: This study proposes a simple, scalable, and general idea and solution for the rapid detection of pathogenic microorganisms and biosecurity monitoring. By designing an electrochemical/optical signal probe and integrating CRISPR/Cas technology and recombinase polymerase amplification, fast and accurate detection of pathogenic microorganisms is achieved.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Analytical
Muhammad Sohail, Siying Xie, Xing Zhang, Bingzhi Li
Summary: CRISPR/Cas-based analytical procedures have revolutionized the sensing platform and have various applications in the detection of nucleic acid and non-nucleic acid analytes. This review highlights different CRISPR/Cas reporters, signal transducers, and sensing strategies, as well as the challenges and requirements for their practical use.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Yuxin Zhang, Yunping Wu, Yanfang Wu, Yangyang Chang, Meng Liu
Summary: The increasing demand for developing biosensors in various fields poses challenges on sensor performance requirements such as sensitivity, selectivity, and operating costs. CRISPR-Cas systems, known as molecular scissors, are highly accurate in cleaving specific DNA and RNA sequences, and recent studies have shown the establishment of versatile biosensing platforms based on CRISPR-Cas. The review introduces the general characteristics and mechanisms of class II CRISPR-Cas systems, highlighting their biosensing strategies for detecting a wide range of nucleic acid and non-nucleic acid analytes.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Joon Soo Park, Kuangwen Hsieh, Liben Chen, Aniruddha Kaushik, Alexander Y. Trick, Tza-Huei Wang
Summary: The digital-enhanced CRISPR/Cas-assisted one-pot virus detection (deCOViD) is a novel assay that allows qualitative and quantitative detection of SARS-CoV-2 in a very short time with high sensitivity and a broad dynamic range. It outperforms current benchtop-based counterparts and represents one of the fastest and most sensitive CRISPR/Cas-assisted SARS-CoV-2 detection methods to date.
Review
Biochemical Research Methods
Mingzhu Yuan, Ronghua Ding, Shuaiyin Chen, Guangcai Duan
Summary: The CRISPR/Cas system provides a rapid and accurate nucleic acid detection method for on-site POCT, with features such as short detection cycles, low cost, and high sensitivity, which are crucial in interrupting outbreaks of infectious diseases.
ACS SYNTHETIC BIOLOGY
(2021)
Review
Chemistry, Analytical
Siyu Chen, Bo Gong, Cong Zhu, Chunyang Lei, Zhou Nie
Summary: Nucleic acid molecules possess attractive properties for biosensing and bioimaging due to their high designability and structural predictability. CRISPR-Cas systems, with high programmability and flexibility, recognize and cleave nucleic acid targets and have been engineered as multifunctional tools. Integration of nucleic acids with CRISPR-Cas systems has advanced biosensing and bioimaging methodologies, holding great promise in biochemical research and clinical diagnosis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biophysics
Xinkuan Cheng, Yaru Li, Jun Kou, Dan Liao, Wenlu Zhang, Lijuan Yin, Shuli Man, Long Ma
Summary: CRISPR/Cas tools have shown great potential in biosensing beyond gene editing. However, the application of CRISPR/Cas-based biosensors for non-nucleic acid targets is still in its early stages. This review summarizes the strategies and prospects of CRISPR/Cas toolboxes in recognizing non-nucleic acid targets, providing valuable information for the expansion of these powerful tools into multiple detection fields.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Ziyue Li, Xiong Ding, Kun Yin, Zhiheng Xu, Kumarasen Cooper, Changchun Liu
Summary: The developed EFE electrochemical CRISPR biosensor is a simple, versatile tool for detecting DNA targets in a homogeneous solution phase, achieving sensitive detection without the need for complicated immobilization processing. By leveraging electric field enhancement and diffusivity differences, the sensor provides a robust and sensitive method for nucleic acid-based molecular diagnostics.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biotechnology & Applied Microbiology
Lijuan Fang, Lusen Yang, Mingyue Han, Huimei Xu, Wenshuai Ding, Xuejun Dong
Summary: The CRISPR system, originally discovered in prokaryotes, is an immune mechanism that clears foreign nucleic acids. Its strong ability of gene editing, regulation and detection in eukaryotes has made it extensively applied in basic and applied research. This article reviews the biology, mechanisms and relevance of CRISPR-Cas technology, as well as its applications in the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The CRISPR-Cas nucleic acid detection tools include CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, CRISPR-Cas14, CRISPR nucleic acid amplification detection technology, and CRISPR colorimetric readout detection system. The CRISPR technologies have been utilized in nucleic acid detection, including SARS-CoV-2 detection. Common nucleic acid detection based on CRISPR derivation technology include SHERLOCK, DETECTR, and STOPCovid. The CRISPR-Cas biosensing technology has been widely applied to point-of-care testing (POCT) by targeting recognition of both DNA molecules and RNA molecules.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Rui Ding, Yue Chen, Qiusu Wang, Zhengzhang Wu, Xing Zhang, Bingzhi Li, Lei Lin
Summary: Antibiotics are widely used in various fields, but residual antibiotics and bacterial resistance have become a global challenge that requires attention. Quantum dots are considered an ideal material for the development of efficient antibiotic detection biosensors, offering many advantages and challenges. Monitoring antibiotics is a critical way to limit ecological risks from antibiotic pollution.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Review
Chemistry, Analytical
Muhammad Sohail, Siying Xie, Xing Zhang, Bingzhi Li
Summary: CRISPR/Cas-based analytical procedures have revolutionized the sensing platform and have various applications in the detection of nucleic acid and non-nucleic acid analytes. This review highlights different CRISPR/Cas reporters, signal transducers, and sensing strategies, as well as the challenges and requirements for their practical use.
ANALYTICA CHIMICA ACTA
(2022)
Article
Fisheries
Hongyu Wang, Beixiang Liu, Wei Qin, Zuosheng Jiang, Chengxiang Zhou, Xing Zhang, Bingzhi Li, Xin Huang, Jiachun Ge, Qian Ren
Summary: This study investigated the impact of HPNS on hepatopancreatic function and molecular levels in E. sinensis, revealing changes in the expression of hemocyanin, E75, and Kruppel in HPNS patients, with EsE75 and EsKruppel acting as transcriptional repressors of the hemocyanin gene. These findings provide insights into the etiology and pathogenesis of HPNS.
Article
Chemistry, Multidisciplinary
Yuan Ji, Wanning Gao, Shilin Zhang, Bingzhi Li, He Huang, Xing Zhang
Summary: In this study, we successfully encapsulated glucose oxidase and magnetic nanoparticles using amorphous ZIF-90 as a host material, creating a promising enzymatic cascade catalyst.
Article
Chemistry, Analytical
Bingzhi Li, Bin Zhou, Yue Chen, Xing Zhang, He Huang
Summary: In this study, a new strategy was proposed to sensitively detect transcription factors using T4 DNA ligase protection assay and rolling circle amplification. By preventing the ligation reaction through the binding of T4 DNA ligase to the nick region of dsDNA, the undesired dissociation of TF-DNA complexes was effectively avoided, improving the accuracy of the assay. Through sequence engineering, multiple transcription factors can be detected at low picomolar levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Muhammad Sohail, Bin Xie, Bingzhi Li, He Huang
Summary: The determination of antioxidant activity plays a crucial role in health sciences and food technology as it is directly related to oxidative stress and various diseases. In this study, a Zn-CDs-based bioanalytical probe was developed for the estimation of antioxidant activity index (AAI), providing a more robust and accurate method compared to traditional techniques.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Bingzhi Li, Qiusu Wang, Muhammad Sohail, Xing Zhang, Huan He, Lei Lin
Summary: The blooming of cyanobacteria in aquatic environments can produce various cyanotoxins polluting soil and water, raising major concerns over environmental and human health issues. Microcystins (MCs) are one of the most toxic cyanotoxins, and the monitoring of MCs is critical for environmental protection and public health. Bio-inspired sensors, also known as biosensors, have become an emerging tool for the detection of MCs.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Zhirun Ji, Zhaoyang Shang, Muhammad Sohail, Pei Wang, Bingzhi Li, Xing Zhang, Guoxiang Chen
Summary: In this study, a fluorometric biosensor based on the protamine-mediated regulation of CRISPR/Cas12a collateral cleavage was developed for the sensitive detection of protamine. The biosensor transduces the information of protamine concentration into DNA information and senses it in a signal turn-off manner.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Pei Wang, Xue Zhang, Hui Shen, Qunwei Lu, Bingzhi Li, Xin Liu, Song Gao
Summary: This study developed a point-of-care testing (POCT)-friendly detection method for Enterocytozoon hepatopenaei (EHP) infection by combining recombinase polymerase amplification (RPA) with the CRISPR-Cas12a system. The method showed improved accuracy and reliability by utilizing the robust amplification capacity of RPA and high specificity of CRISPR. By carefully designing crRNA and optimizing the reaction, the one-pot procedure was achieved, solving the compatibility issues between RPA and Cas12a cleavage. The method demonstrated high specificity and sensitivity, making it suitable for POCT environment and having great application value in preventing EHP infection in the shrimp farming industry.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Nanoscience & Nanotechnology
Jie Zheng, Han Xu, Bingzhi Li, Muhammad Sohail, Jingjing Bi, Fuming Zhang, Robert J. Linhardt, He Huang, Xing Zhang
Summary: Based on the Modular-Enzymes Assembly by Spatial Segregation (MASS) strategy, a universal immobilization platform was developed to assemble multiple glycoenzymes in compartmentalized MOF particles, reducing interference and cross-inhibitions. Different glycans, including glycosyl donor, disaccharides, oligosaccharides, and polysaccharides with cofactor regeneration, were efficiently prepared. This robust multienzyme immobilization platform has industrial potential for enzymatic synthesis of more complex glycans.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Xiaochen Ma, Tiying Suo, Furong Zhao, Zhaoyang Shang, Yue Chen, Pei Wang, Bingzhi Li
Summary: This study developed a fluorometric aptasensor for the detection of cadmium ions (Cd(II)) by integrating strand displacement amplification (SDA) and CRISPR/Cas12a technologies. In the absence of Cd(II), the aptamer initiates SDA and activates Cas12a, resulting in a significant increase in fluorescence output. Conversely, the presence of Cd(II) reduces the efficiency of SDA, leading to a notable decrease in fluorescence output. The proposed method enables selective detection of Cd(II) at concentrations of 60 pM and has been validated in real water and rice samples. It holds great promise as a signal-amplified and precise detection platform for Cd(II), potentially transforming current hazard detection practices in food samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Bin Zhou, Runlin Yang, Muhammad Sohail, Xiaoxue Kong, Xing Zhang, Ninghua Fu, Bingzhi Li
Summary: CRISPR is revolutionizing biosensing technology with its programmability, sensitivity, and specificity. While most CRISPR-based biosensors are developed using Cas12 and Cas13, the biosensing potential of the newly discovered Cas14 is not yet fully understood. This study introduces HARRY, a fluorometric biosensor that utilizes the specificity of Cas14a for versatile aptasensing. HARRY shows improved sensitivity and versatility compared to Cas12a-based aptasensors, detecting various targets at low-nanomolar levels. The study suggests that the detection limit of HARRY is correlated to the binding affinities of aptamers. These findings unlock the potential of Cas14a in CRISPR-based biosensing and inspire further development in this field.
Review
Environmental Sciences
Muhammad Sohail, Zunaira Urooj, Sobia Noreen, Mirza Muhammad Faran Ashraf Baig, Xing Zhang, Bingzhi Li
Summary: Micro-and nanoplastics (M/NPs) are emerging pollutants released into the food, beverages, and environment from plastic products. Detection of M/NPs in food and beverages is crucial for preventing their harmful effects. However, current analytical strategies have limitations and detecting M/NPs accurately is challenging due to their varying content and contamination routes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Furong Zhao, Pei Wang, Haoxuan Wang, Sirui Liu, Muhammad Sohail, Xing Zhang, Bingzhi Li, He Huang
Summary: The spread of monkeypox virus (MPXV) from Central and West Africa to other regions has caused global alarm. A new assay combining recombinase-aided amplification (RAA) and CRISPR/Cas12a technology has been proposed, showing low detection limits and high selectivity for MPXV. A kit with lateral flow strips was developed for on-site screening, enabling easy and rapid MPXV detection without advanced equipment. This assay has the potential to be used for on-site surveillance of MPXV in emerging viral outbreaks.
ANALYTICAL METHODS
(2023)
Article
Materials Science, Biomaterials
Siying Xie, Cheng Qin, Furong Zhao, Zhaoyang Shang, Pei Wang, Muhammad Sohail, Xing Zhang, Bingzhi Li
Summary: Researchers have developed a label-free and low-background CRISPR/Cas12a signal reporter by integrating DNA-templated copper nanoclusters (DNA-CuNCs) and exonuclease I (EXO I). Based on this reporter, they have successfully developed a biosensor called CRISPR-CNS, which has excellent performance and sensitivity in nucleic acid detection.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
