Article
Biochemistry & Molecular Biology
Anqi Zhang, Daisuke Nakano, Wararat Kittikulsuth, Yuka Yamashita, Akira Nishiyama
Summary: The study examined the effects of the SGLT2 inhibitor luseogliflozin on glucose dynamics in the proximal tubules, finding that blood glucose levels do not alter glucose influx or efflux kinetics. This is because glucose enters the cells through the established bidirectional transporter GLUT2 on the basolateral membrane.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Carlotta Mayer, Sophie Nehring, Michael Kuecken, Urska Repnik, Sarah Seifert, Aleksandra Sljukic, Julien Delpierre, Hernan Morales-Navarrete, Sebastian Hinz, Mario Brosch, Brian Chung, Tom Karlsen, Meritxell Huch, Yannis Kalaidzidis, Lutz Brusch, Jochen Hampe, Clemens Schafmayer, Marino Zerial
Summary: Hepatocytes dynamically respond to bile acids and bile flow, but little is known about their response to intraluminal pressure. This study reveals that hepatocytes form apical bulkheads in response to obstructed bile flow, which protect bile canaliculi against elevated pressure. The loss of apical bulkheads leads to abnormally dilated canaliculi, highlighting the importance of canalicular pressure in liver diseases.
Review
Physiology
Shivangi Agarwal, Yashwanth R. Sudhini, Onur K. Polat, Jochen Reiser, Mehmet M. Altintas
Summary: The kidneys, responsible for maintaining body homeostasis, exhibit complexity in function and structure that require diverse cell types and precise cell markers for identification. Recent advancements in labeling and imaging techniques have allowed for efficient in vivo cell identification, although discrepancies in cell type-specific markers exist among different studies. There is a need for more precise definition of cell markers to ensure accurate interpretation of data.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Cell Biology
Fengping Zheng, Donge Tang, Shanshan Li, Zhifeng Luo, Yueqi Song, Yinxin Huang, Qing Gan, Hanyong Liu, Xinzhou Zhang, Dongzhou Liu, Qingwen Wang, Zuying Xiong, Yong Dai
Summary: This study used laser capture microdissection (LCM) to analyze the kidney in lupus nephritis (LN). The glomerulus and interstitial were identified as major sites of immune disturbance and regulation. Additionally, several hub genes correlated with immune cell infiltration were identified. ALB, CTSB, LCN2, A2M, CDC42, VIM, LTF, and CD14 were suggested as candidate biomarkers based on their correlation with clinical indexes.
INFLAMMATION RESEARCH
(2023)
Article
Plant Sciences
Jin-Quan Zhao, Zhen Zhao, Cai Zhang, Jia-Xing Sun, Feng-Jie Liu, Ting Yu, Yan Jiang, Hui-Jun Li
Summary: Epimedii Folium (EF) is a traditional Chinese medicine known for its ability to tonify kidney yang and strengthen bones and muscles. However, studies have suggested that EF may also pose a potential risk of cholestatic liver damage. This study aimed to evaluate the risk of EF-induced cholestasis and explore the underlying mechanisms. The results showed that long-term oral administration of EF caused cholestatic liver injury in mice, potentially through disruption of bile acid metabolism and transport.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Maja Danic, Nebojsa Pavlovic, Slavica Lazarevic, Bojan Stanimirov, Sasa Vukmirovic, Hani Al-Salami, Armin Mooranian, Momir Mikov
Summary: Researchers have found that the interaction between gut microbiota and bile acids may have significant effects on the efficacy of simvastatin, a lipid-lowering drug. They observed the bioaccumulation and biotransformation of simvastatin in probiotic bacteria, suggesting that these processes may contribute to altered drug availability and therapeutic effect.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Medicine, Research & Experimental
Di Liu, Yan Du, Fei-Yang Jin, Xiao-Ling Xu, Yong-Zhong Du
Summary: Kidney diseases, including AKI and CKD, are major global public health concerns with limited effective drug therapies. Kidney-targeted drug delivery is a potential strategy to improve therapeutic efficacy and reduce side effects. This review discusses renal cell-targeted formulations for AKI and CKD, providing biodistribution data and proposing future research directions.
MOLECULAR PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Yanbao Xin, Yue Hou, Xiuxiu Cong, Huizhu Tan, Jialiang Wang, Kuirong Mao, Xin Wang, Feiqi Liu, Yong-Guang Yang, Tianmeng Sun
Summary: Combining polyethylene glycol (PEG) with nanoparticles has become a popular method to enhance drug delivery efficiency in vivo. This study reveals that the type of PEG end-group and kidney function damage significantly influence the accumulation and distribution of PEGylated nanoparticles in kidneys, providing insights into their interactions in vivo.
Article
Biology
Rie Sonoi, Yoshihisa Hagihara
Summary: The study found that Entacapone (ENT) induced liver injury by affecting the structure of tight junctions in hepatocytes, leading to bile canaliculi dilation and cell apoptosis; forskolin (FOR) or sodium taurocholate promoted TJ formation, suppressed bile canaliculi dynamic changes, and inhibited TJ disruption and cell apoptosis.
Review
Chemistry, Medicinal
Hao Li, Wenni Dai, Zhiwen Liu, Liyu He
Summary: Diabetic kidney disease is a major cause of end-stage kidney disease worldwide, and new drug delivery strategies and targeted therapies are urgently needed.
Article
Biochemical Research Methods
Yan Jin, Yang Li, Eric D. Eisenmann, William D. Figg, Sharyn D. Baker, Alex Sparreboom, Shuiying Hu
Summary: A novel UHPLC-MS/MS method was developed and validated for the quantification of GCDCA-S and CDCA-24G in mouse and human plasma, and successfully applied to study the activity of OATP1B1/OATP1B3 and the effects of inhibitors.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Cell Biology
Jun Wei Chan, Claire Wen Ying Neo, Soumita Ghosh, Hyungwon Choi, Su Chi Lim, E. Shyong Tai, Adrian Kee Keong Teo
Summary: The study reveals the involvement of HNF1A in kidney development and function, and identifies SLC51B as a target gene responsible for E1S uptake in human proximal tubule cells. Dysfunction of this process in MODY3 patients may contribute to the development of renal disease.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Peter A. Friedman, W. Bruce Sneddon, Tatyana Mamonova, Carolina Montanez-Miranda, Suneela Ramineni, Nicholas H. Harbin, Katherine E. Squires, Julia Gefter, Clara E. Magyar, David R. Emlet, John R. Hepler
Summary: This study identified a novel role for RGS14 in hormone-sensitive phosphate transport, suggesting that changes in RGS14 function or abundance may contribute to hormone resistance and hyperphosphatemia observed in kidney diseases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Robin Durnik, Lenka Sindlerova, Pavel Babica, Ondrej Jurcek
Summary: This paper provides a systematic review on the transport processes and enterohepatic circulation of bile acids (BAs). It focuses on describing specific or less-specific BA transport proteins and their localization, and provides essential information about the properties, flow, metabolism, and functions of BAs. The transport processes are described step by step, accompanied by descriptions of major proteins involved, and spillage of BAs into systemic circulation and urine excretion are also discussed. The review also highlights the importance of less-studied areas in the development of BA-related drugs.
Article
Radiology, Nuclear Medicine & Medical Imaging
Catherine M. Pastor, Florian Joly, Valerie Vilgrain, Philippe Millet
Summary: In hepatobiliary imaging, the liver extraction ratios of hepatobiliary agents are correlated with hepatocyte concentrations. In cholestatic livers, absence of Bopta in bile canaliculi results in increased hepatocyte concentrations compared to normal livers. This new understanding expands the interpretation of clinical liver images.
EUROPEAN RADIOLOGY EXPERIMENTAL
(2021)
Article
Biochemistry & Molecular Biology
Tim Brecklinghaus, Wiebke Albrecht, Franziska Kappenberg, Julia Duda, Nachiket Vartak, Karolina Edlund, Rosemarie Marchan, Ahmed Ghallab, Cristina Cadenas, Georgia Gunther, Marcel Leist, Mian Zhang, Iain Gardner, Jorg Reinders, Frans G. M. Russel, Alison J. Foster, Dominic P. Williams, Amruta Damle-Vartak, Melanie Grandits, Gerhard Ecker, Naim Kittana, Jorg Rahnenfuehrer, Jan G. Hengstler
Summary: A method was introduced to assess the risk of human drug induced liver injury based on oral doses and blood concentrations of drugs using in vitro/in silico approaches. The addition of an assay that measures the inhibition of bile acid export carriers improved the differentiation of hepatotoxic and non-hepatotoxic compounds. Integration of the CMFDA assay with an in vitro test battery enhanced the separation of hepatotoxic from non-hepatotoxic compounds.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Gastroenterology & Hepatology
Jelena Remetic, Ahmed Ghallab, Zaynab Hobloss, Lisa Brackhagen, Reham Hassan, Maiju Myllys, Richard Radun, Veronika Mlitz, Ci Zhu, Maximilian Baumgartner, Waltraud C. Schrottmaier, Marion Mussbacher, Gerald Timelthaler, Hubert Scharnagl, Tatjana Stojakovic, Alice Assinger, Claudia D. Fuchs, Jan G. Hengstler, Michael Trauner
Summary: This study found that absence of Bsep and cholestasis impairs LPS clearance, exacerbating hepatic inflammation. The clearance of LPS is compromised in Bsep KO mice, affecting its uptake into hepatocytes and secretion into canaliculi, resulting in more severe liver injury.
Article
Gastroenterology & Hepatology
Ahmed Ghallab, Reham Hassan, Ute Hofmann, Adrian Friebel, Zaynab Hobloss, Lisa Brackhagen, Brigitte Begher-Tibbe, Maiju Myllys, Joerg Reinders, Nina Overbeck, Selahaddin Sezgin, Sebastian Zuehlke, Abdel-Latif Seddek, Walaa Murad, Tim Brecklinghaus, Franziska Kappenberg, Joerg Rahnenfuehrer, Daniela Gonzalez, Christopher Goldring, Ian M. Copple, Rosemarie Marchan, Thomas Longerich, Mihael Vucur, Tom Luedde, Stephan Urban, Ali Canbay, Thomas Schreiter, Michael Trauner, Jephte Y. Akakpo, Mojtaba Olyaee, Steven C. Curry, Jan-Peter Sowa, Hartmut Jaeschke, Stefan Hoehme, Jan G. Hengstler
Summary: This study revealed the mechanism by which acetaminophen overdose causes a breach of the blood-bile barrier, resulting in futile cycling of BAs and hepatocyte death.
JOURNAL OF HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Annika R. P. Schneider, Carolin Schneider, Kai Markus Schneider, Vanessa Baier, Steffen Schaper, Christian Diedrich, Katrin Coboeken, Hannah Mayer, Wenyi Gu, Jonel Trebicka, Lars M. Blank, Rolf Burghaus, Joerg Lippert, Daniel J. Rader, Christoph A. Thaiss, Jan-Frederik Schlender, Christian Trautwein, Lars Kuepfer
Summary: The study aims to develop a prognostic score for predicting decompensation risk in patients with compensated cirrhosis. The EPOD score, based on platelet count, albumin, and bilirubin concentration, outperforms existing cirrhosis scores and accurately predicts the probability of decompensation within a 3-year follow-up.
LIVER INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Patrick Schriek, Alan C. Ching, Nagaraj S. Moily, Jessica Moffat, Lynette Beattie, Thiago M. Steiner, Laine M. Hosking, Joshua M. Thurman, V. Michael Holers, Satoshi Ishido, Mireille H. Lahoud, Irina Caminschi, William R. Heath, Justine D. Mintern, Jose A. Villadangos
Summary: Marginal zone B cells acquire dendritic cell-like properties through the trogocytic acquisition of MHC II-C3 complexes from conventional dendritic cells.
Article
Health Care Sciences & Services
Catherine Bjerre Collin, Tom Gebhardt, Martin Golebiewski, Tugce Karaderi, Maximilian Hillemanns, Faiz Muhammad Khan, Ali Salehzadeh-Yazdi, Marc Kirschner, Sylvia Krobitsch, Lars Kuepfer
Summary: The future development of personalized medicine depends on data exchange and analysis, and computational modeling plays a key role. However, there are challenges to implementing these models clinically, which require clear guidelines. This article discusses computational models in personalized medicine and provides relevant challenges, as well as implementation recommendations.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Irina Kube, Manuela Kowalczyk, Ute Hofmann, Ahmed Ghallab, Jan Georg Hengstler, Dagmar Fuehrer, Denise Zwanziger
Summary: Women are more prone to develop hypothyroidism or cholesterol gallstones. Recent research has discovered a new pathological link between thyroid hormone deficiency and cholesterol gallstones. This study investigated the impact of thyroid hormone deficiency on cholesterol gallstone formation in female mice, and found that it promotes the formation of cholesterol gallstones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Lilija Wehling, Liam Keegan, Paula Fernandez-Palanca, Reham Hassan, Ahmed Ghallab, Jennifer Schmitt, Yingyue Tang, Maxime Le Marois, Stephanie Roessler, Peter Schirmacher, Ursula Kummer, Jan G. Hengstler, Sven Sahle, Kai Breuhahn, Matthew A. Quinn
Summary: This study reveals the localization mechanism of the Hippo signaling pathway in the nucleus and cytoplasm through experimental and modeling analysis. Treatment of hepatocyte-derived cells leads to nuclear enrichment of YAP but not TAZ, which is related to the interaction between reactive oxygen species, protein kinase B, and YAP.
Review
Microbiology
Malcolm S. Duthie, Bruna A. S. Machado, Roberto Badaro, Paul M. Kaye, Steven G. Reed
Summary: Leishmania parasites cause various diseases and only a few vaccine candidates have advanced to clinical trials. RNA technology shows potential benefits in addressing the challenges of Leishmania vaccine development. Strategies, including controlled human infection models and initial use in a therapeutic setting, can efficiently evaluate the efficacy of Leishmania vaccines.
Article
Toxicology
Andrea Gerdemann, Benedikt Cramer, Gisela H. Degen, Jannik Veerkamp, Georgia Guenther, Wiebke Albrecht, Matthias Behrens, Melanie Esselen, Ahmed Ghallab, Jan G. Hengstler, Hans-Ulrich Humpf
Summary: This study analyzed the metabolites formed by mouse, rat, and human primary hepatocytes after treatment with different concentrations of Aflatoxin B-1 (AFB(1)). The results showed species-specific differences in the metabolism of AFB(1) and provided insights into the toxification and detoxification mechanisms of AFB(1).
ARCHIVES OF TOXICOLOGY
(2023)
Article
Toxicology
Noha Abdelmageed, Wael Ahmed-Anwar Twafik, Osama Abdel-Raouf Morad, Mohie Haridy, Reham Hassan, Madeha Ahmed, Hesham Youssef El-Zorba, Hossny Awad El-banna, Abdel-latief Seddek, Ahmed Ghallab, Samy Abdel-Raouf Fahim Morad
Summary: The study aimed to evaluate the potential protective effect of the nootropic agent vinpocetine against the adverse effects of chloroquine (CQ) and hydroxychloroquine (HCQ) on the heart. The results showed that vinpocetine significantly mitigated CQ-induced cardiac damage and oxidative stress. Therefore, vinpocetine could be used as an adjuvant therapy for CQ/HCQ applications.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Parasitology
Ritika Sengupta, Madhurima Roy, Nidhi S. Dey, Paul M. Kaye, Mitali Chatterjee
Summary: PKDL is a dermatological sequel of VL, characterized by hypopigmentation and the loss of melanocytes possibly caused by immune dysregulation and inflammation.
TRENDS IN PARASITOLOGY
(2023)
Article
Cell Biology
Raly James Perez Custodio, Zaynab Hobloss, Maiju Myllys, Reham Hassan, Daniela Gonzalez, Joerg Reinders, Julia Bornhorst, Ann-Kathrin Weishaupt, Abdel-latif Seddek, Tahany Abbas, Adrian Friebel, Stefan Hoehme, Stephan Getzmann, Jan G. Hengstler, Christoph van Thriel, Ahmed Ghallab
Summary: Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD) is the most common chronic liver disease in Western countries. This study found that MASLD has an effect on brain histology, cognitive functions, and neurotransmitters.
Article
Biotechnology & Applied Microbiology
Xiaowei Huang, Andreas K. Nussler, Marie K. Reumann, Peter Augat, Maximilian M. Menger, Ahmed Ghallab, Jan G. Hengstler, Tina Histing, Sabrina Ehnert
Summary: The present study established and validated a finite element model (FEM) to predict the mechanical properties of mice tibiae. The FEM showed good correlation with biomechanical data obtained from bending tests. By reducing the need for animal experiments, the FEM can contribute to reducing the number of research animals in bone biomechanics studies.
BIOENGINEERING-BASEL
(2022)
Article
Gastroenterology & Hepatology
Christian H. Holland, Ricardo O. Ramirez Flores, Maiju Myllys, Reham Hassan, Karolina Edlund, Ute Hofmann, Rosemarie Marchan, Cristina Cadenas, Joerg Reinders, Stefan Hoehme, Abdel-latif Seddek, Steven Dooley, Verena Keitel, Patricio Godoy, Brigitte Begher-Tibbe, Christian Trautwein, Christian Rupp, Sebastian Mueller, Thomas Longerich, Jan G. Hengstler, Julio Saez-Rodriguez, Ahmed Ghallab
Summary: The study revealed consistently regulated genes between mouse models and human chronic liver diseases, with the highest recall and precision observed in the 12-month CCl4-induced damage and Western diet model. Up-regulated genes in the chronic CCl4 model were enriched in inflammatory and developmental processes, predominantly mapping to specific liver cell types, while down-regulated genes were associated with metabolic processes in hepatocytes.
HEPATOLOGY COMMUNICATIONS
(2022)