Article
Food Science & Technology
Shohreh Nasiri Poroj, Mohaddeseh Larypoor, Mohammad Reza Fazeli, Farid Shariatmadari
Summary: This study investigated the reduction of aflatoxin by yeast strains isolated from fermented products and titanium dioxide nanoparticles. The selected yeast strains showed probiotic and antimicrobial activity, as well as enhanced aflatoxin reduction when combined with TiO2-NPs.
FOOD SCIENCE & NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Zhen Lin, Ping Luo, Dongmin Huang, Yulian Wu, Fangping Li, Huazhong Liu
Summary: The study reveals that exposure to ACR leads to impairment of oxidative-reductive balance, energy metabolism, lipid metabolism, nucleotide metabolism, and ribosome function in yeast cells. In response to acute ACR damage, yeast cells exhibit upregulation of glutathione synthesis, acceleration of protein degradation, and initiation of autophagy flux.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Mrinal Sharma, Prranshu Yadav, Ankita Doshi, Hemang D. Brahmbhatt, C. Ratna Prabha
Summary: The study examined the effects of six double mutants derived from the ubiquitin mutant UbEP42 on cell functions, finding that the double mutation L50P-I61T had the most detrimental impact. Overall, these double mutations made cells more sensitive, and the negative effects under certain conditions could be compensated for by each other.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Water Resources
Mohaddeseh Zahmatkesh Anbarani, Afsaneh Esmaeili Nasrabadi, Ziaeddin Bonyadi
Summary: The aim of this study was to evaluate the efficiency of S. cerevisiae in removing PS from aqueous solutions. BBD was used to determine the optimal removal conditions. The maximum PS removal efficiency was 98.81% under optimized conditions. Based on the results, it can be concluded that S. cerevisiae can be used as a natural and environmentally friendly biocoagulant to remove PS.
APPLIED WATER SCIENCE
(2023)
Article
Biology
Carles Pons, Jolanda van Leeuwen
Summary: This study comprehensively characterizes dispensable essential genes in Saccharomyces cerevisiae by compiling data from multiple studies. It reveals the high functional modularity of the bypass suppression network through interactions with suppressor genes. Moreover, it also identifies associations between the mutational landscape of yeast strains and the dispensability of essential genes.
LIFE SCIENCE ALLIANCE
(2023)
Article
Food Science & Technology
Jitka Laurent, Nore Struyf, An Bautil, Albina Bakeeva, Mikolaj Chmielarz, Marika Lyly, Beatriz Herrera-Malaver, Volkmar Passoth, Kevin J. Verstrepen, Christophe M. Courtin
Summary: A diet low in FODMAPs can help reduce symptoms in patients with irritable bowel syndrome, but avoiding cereal grain products may lead to nutritional deficiencies. Developing low-FODMAP, high-fiber cereal grain products using K. marxianus in bread production can effectively reduce fructan levels and maintain fructan and fructose levels below the threshold for low-FODMAP products.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Genetics & Heredity
Raquel Sorribes-Dauden, Maria Teresa Martinez-Pastor, Sergi Puig
Summary: Iron is essential for eukaryotic organisms, and its deficiency can lead to anemia. Yeast cells use the Ccc1 transporter to detoxify and store excess iron. Deletion of the Nt domain of Ccc1 can increase iron accumulation in cells.
Article
Immunology
Francesca Soutter, Dirk Werling, Matthew Nolan, Tatiana Kuster, Elizabeth Attree, Virginia Marugan-Hernandez, Sungwon Kim, Fiona M. Tomley, Damer P. Blake
Summary: A cheap and easy-to-produce oral vaccine has been developed using Saccharomyces cerevisiae yeast expressing Eimeria tenella antigens. The vaccine effectively reduces the replication of Eimeria tenella and improves the growth of chickens, making it an important tool for controlling coccidiosis in chickens, particularly in low- and middle-income countries.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lu Meng, Xu Yang, Xue Lin, Huan-Yuan Jiang, Xiao-Ping Hu, Si-Xin Liu
Summary: The study found that overexpression of SNF1 in baker's yeast enhances cell tolerance and fermentation capacity during freezing by upregulating proteasome, altering carbon source and protectant molecule metabolism, and changing cell membrane components. Before freezing, SNF1 overexpression regulated levels of specific amino acids, glycerol, fatty acids, and phospholipids, while after freezing, it maintained high levels of certain metabolites contributing to freezing resistance. This research provides insights into cellular responses of baker's yeast to freezing and potential for developing low-temperature resistant strains.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
Bazile Ravoityte, Juliana Luksa, Ralf Erik Wellinger, Saulius Serva, Elena Serviene
Summary: This study analyzed the impact of L-A virus on gene expression in yeast hosts using high-throughput RNA sequencing data. It was found that the presence of L-A virus resulted in moderate alterations in gene expression, particularly in genes involved in ribosome biogenesis. Additionally, the transcriptional responses to L-A maintenance were similar to those induced by stress or nutrient availability. The study also identified yeast transcriptional regulators that modulate the levels of L-A dsRNA in cells.
Article
Biotechnology & Applied Microbiology
Pedro Pais, Jorge Oliveira, Vanda Almeida, Melike Yilmaz, Pedro T. Monteiro, Miguel C. Teixeira
Summary: The study found that S. cerevisiae var. boulardii exhibited higher survival capability and acetate biosynthesis in an intestinal-like medium compared to S. cerevisiae, possibly related to its probiotic activity. Analysis of a genomic database for S. cerevisiae var. boulardii suggested a potential link between variations in transcription factor binding sites and transcriptomic patterns.
Review
Biotechnology & Applied Microbiology
R. Sahaya Glingston, Jyoti Yadav, Jitika Rajpoot, Neha Joshi, Shirisha Nagotu
Summary: Yeast has been proven to be a valuable model system for studying viral infection and expression of viral proteins, advancing our understanding of important viruses. Through high-throughput analysis, genetic manipulation, and its compact genome, yeast provides insights into various aspects related to virus replication.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chuan Yang, Aoxue Dong, Li Deng, Fang Wang, Junfeng Liu
Summary: Fermentation processes are greatly influenced by thermal changes, particularly in the yield of lipid products; however, the changes in lipid metabolism in response to low temperatures are not well understood. This study utilized transcriptome and lipidomic analysis to investigate alterations in lipid metabolism under low and optimal temperatures. Transcriptome analysis revealed that ribosome biogenesis and carbon metabolism pathways were significantly induced by the drop in temperature. The data also indicated that yeast cells adjust membrane fluidity by modifying the content of different lipid species, rather than the unsaturation level of corresponding fatty acids. The core regulator of energy metabolism in yeast's adaptation to low temperature was identified as Snf1 through transcriptome analysis of glucose-energy metabolism. These findings enhance our understanding of carbon metabolism in Saccha-romyces cerevisiae under cold stress and provide valuable insights for the production of high-value lipid products through metabolic engineering.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Applied
Sofia F. Reis, Susana Messias, Rita Bastos, Vitor J. Martins, Viviana G. Correia, Benedita A. Pinheiro, Lisete M. Silva, Angelina S. Palma, Manuel A. Coimbra, Elisabete Coelho
Summary: Brewing practice involves reusing the same yeast for subsequent fermentations. The yeast used for Lager beer (Saccharomyces pastorianus) is widely reused without changes in fermentation performance. However, the yeast used for Ale beer (Saccharomyces cerevisiae) is either partially or not reused due to poor performance. The study found that cells modulate their wall polysaccharides to increase cell wall strength.
CARBOHYDRATE POLYMERS
(2023)
Article
Plant Sciences
Bruna B. F. Cal, Luana B. N. Araujo, Brenno M. Nunes, Claudia R. da Silva, Marcia B. N. Oliveira, Bianka O. Soares, Alvaro A. C. Leitao, Marcelo de Padula, Debora Nascimento, Douglas S. A. Chaves, Rachel F. Gagliardi, Flavio J. S. Dantas
Summary: The ethanolic extract of Petiveria alliacea L. showed cytotoxic and mutagenic effects on Saccharomyces cerevisiae, possibly mediated by oxidative lesions.
Article
Genetics & Heredity
Roberto F. Nespolo, Carlos A. Villarroel, Christian Oporto, Sebastian M. Tapia, Franco Vega-Macaya, Kamila Urbina, Matteo De Chiara, Simone Mozzachiodi, Ekaterina Mikhalev, Dawn Thompson, Luis F. Larrondo, Pablo Saenz-Agudelo, Gianni Liti, Francisco A. Cubillos
Article
Biotechnology & Applied Microbiology
Wladimir Mardones, Carlos A. Villarroel, Valentina Abarca, Kamila Urbina, Tomas A. Pena, Jennifer Molinet, Roberto F. Nespolo, Francisco A. Cubillos
Summary: Experimental evolution can rapidly rebuild the brewing domestication process in wild yeast, showcasing the complex genetics of adaptation in populations. The study demonstrates the rapid evolution of traits in response to selection pressure, including genetic variants, genomic rearrangements, and altered gene expression contributing to enhanced stress tolerance and fermentation efficiency.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yeney Lauzurique, Fernando G. Fermoso, Nicolas Sanchez, Alejandra Castillo, Natalia Valdes, Mario Tello, Ricardo Salazar, Veronica Garcia, Cesar Huilinir
Summary: The study shows that the addition of moderate concentrations of fly ash can enhance specific methane production in the anaerobic digestion of winery wastewater, but high concentrations may inhibit methane generation and impact bacterial communities.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Carlos A. Villarroel, Macarena Bastias, Paulo Canessa, Francisco A. Cubillos
Summary: This study utilized an F1 hybrid of two contrasting S. cerevisiae strains to investigate cis factors involved in gene expression regulation divergence under nitrogen scarcity. Variability in allele-specific expression and accessibility was observed, with a significant portion specific to deficient nitrogen environments. Events of allelic bias in gene expression and transcription factor binding were discovered under nitrogen scarcity, suggesting a cis x environment-specific response.
Article
Environmental Sciences
Yeney Lauzurique, Lidia Carolina Espinoza, Cesar Huilinir, Veronica Garcia, Ricardo Salazar
Summary: Winery wastewater, the largest waste stream in the wine industry, contains high amounts of organic matter that vary with time and wine production levels. Anodic oxidation using a boron-doped diamond electrode achieved 85% mineralization of the wastewater, while the mixed metal oxides electrode only achieved 2.52% degradation.
Article
Biochemistry & Molecular Biology
Carlos A. Villarroel, Angelica Gonzalez-Gonzalez, Jeniffer K. Alvarez-Baca, Pablo Villarreal, Gabriel Ballesteros, Christian C. Figueroa, Francisco A. Cubillos, Claudio C. Ramirez
Summary: This study reports a highly complete de novo draft genome of the English grain aphid, providing insights into gene family evolution, resistance mechanisms, and invasive success in agroecosystems.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sebastian M. Tapia, Roberto Perez-Torrado, Ana Cristina Adam, Laura G. Macias, Eladio Barrio, Amparo Querol
Summary: This study reveals the differences in production of rose-like aroma compounds, phenylethanol (PE) and phenylethyl acetate (PEA), among different yeast species. The variations in the transcriptional activator Aro80p directly impact the expression of genes related to aroma compound biosynthesis, leading to increased production. This finding suggests potential alternatives for enhancing the concentration of rose-like aroma compounds in wine.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Matias Aguilera, Sofia Martinez, Mario Tello, Maria Jose Gallardo, Veronica Garcia
Summary: Foodborne diseases are a significant concern in public health due to their impact and the number of affected individuals each year. The food industry has implemented microbiological control plans, but new control systems are needed due to high prevalence. This study suggests that the use of bacteriophages can reduce contamination of Salmonella in chicken meat.
Article
Biotechnology & Applied Microbiology
Sebastian M. Tapia, Roberto Perez-Torrado, Ana Cristina Adam, Laura G. Macias, Eladio Barrio, Amparo Querol
Summary: The use of unconventional yeast species in fermentations has gained attention for solving problems in the alcoholic beverage industry. Saccharomyces kudriavzevii, a species with unique fermentative traits, has been observed to produce higher amounts of aroma compounds in wines, such as phenylethanol. However, it has been found that the presence of a specific variant of the ARO4 enzyme in S. kudriavzevii reduces phenylethanol production compared to Saccharomyces cerevisiae, potentially due to increased feedback inhibition caused by amino end changes in Aro4p.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Ismael Soto, Raul Zamorano-Illanes, Raimundo Becerra, Pablo Palacios Jativa, Cesar A. Azurdia-Meza, Wilson Alavia, Veronica Garcia, Muhammad Ijaz, David Zabala-Blanco
Summary: This article proposes a novel method for detecting COVID-19 in an underground channel using VLC and ML. The mathematical models of COVID-19 DNA gene transfer in regular square constellations using a CSK/QAM-based VLC system are presented. ML algorithms are utilized to classify the bands in electrophoresis samples to search for the optimal model. Results show that the square constellation N = 2(2i )x 2(2i), (i = 3) yields the highest profit and XGBoost achieves the best accuracy of 96.03% and a recall of 99% for positive values among all the models tested.
Article
Biology
Sebastian M. Tapia, Laura G. Macias, Roberto Perez-Torrado, Noemi Daroqui, Paloma Manzanares, Amparo Querol, Eladio Barrio
Summary: Through comparative genomic analysis, researchers discovered horizontal gene transfer events in wild Saccharomyces yeasts and identified the function of two related genes, DGD1 and DGD2. The results suggest that the presence of these genes could be attributed to an early gene transfer event and contributed to the development of new traits in the ancestor of the Saccharomyces genus.
Article
Engineering, Environmental
Miguel A. Sandoval, L. Carolina Espinoza, Oscar Coren, Veronica Garcia, Rosalba Fuentes, Abdoulaye Thiam, Ricardo Salazar
Summary: This study investigated the treatment of industrial cattle slaughterhouse wastewater using anodic oxidation and electrocoagulation processes, and the results showed that electrochemical techniques were effective in removing organic matter, achieving complete discoloration and disinfection.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Proceedings Paper
Optics
Carlos Fernandez-Grandon, Ismael Soto, David Zabala-Blanco, Wilson Alavia, Veronica Garcia
Summary: This article discusses the implementation of algorithms for automatic diagnosis of lung diseases such as COVID-19 and Pneumonia from chest X-rays, achieving a maximum accuracy of 93.73% for SVM classifier.
2021 THIRD SOUTH AMERICAN COLLOQUIUM ON VISIBLE LIGHT COMMUNICATIONS (SACVLC 2021)
(2021)
Review
Engineering, Environmental
Yeney Lauzurique, Silvio Montalvo, Ricardo Salazar, Veronica Garcia, Cesar Huilinir
Summary: This document explores the potential use of fly ash as an additive to enhance anaerobic digestion processes, reviewing its impact on methane production and biodegradation. The importance of trace elements for methanogenic archaea, possible mechanisms for improving anaerobic digestion, and the limitations and prospects of using fly ash on an industrial scale are also discussed.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Panagiota Filippou, Xavier Zarza, Chrystalla Antoniou, Toshihiro Obata, Carlos A. Villarroel, Ioannis Ganopoulos, Vaggelis Harokopos, Gholamreza Gohari, Vassilis Aidinis, Panagiotis Madesis, Anastasis Christou, Alisdair R. Fernie, Antonio F. Tiburcio, Vasileios Fotopoulos
Summary: The study found that salt-tolerant genotypes displayed lower levels of oxidative damage after salt treatment, while salt-sensitive genotypes showed higher oxidative responses. Metabolomics data revealed differences in responses to stress among different genotypes, while global transcriptomic analysis identified previously undiscovered gene expression differences related to salinity.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)