Article
Agriculture, Dairy & Animal Science
Tingting Zhu, Qicun Zhou, Zheng Yang, Yingying Zhang, Jiaxiang Luo, Xiangsheng Zhang, Yuedong Shen, Lefei Jiao, Douglas R. Tocher, Min Jin
Summary: This study evaluated the effects of different dietary cholesterol levels on the growth, cholesterol metabolism, and gene expression in female swimming crabs. The results showed that crabs fed a diet with 1.00% cholesterol exhibited the best growth performance, and tissue cholesterol concentrations were positively correlated with dietary cholesterol level. Functional fatty acid contents and hormone levels related to molting were also influenced by dietary cholesterol. The study concluded that an optimal dietary level of cholesterol benefitted the accumulation of functional fatty acids, regulated lipid metabolism, and improved molting and growth in swimming crabs.
Article
Parasitology
Lu Liu, Zongshan Zhang, Hui Liu, Shengnan Zhu, Taoxun Zhou, Chunqun Wang, Min Hu
Summary: In this study, the haemozoin formation in the blood-sucking nematode Haemonchus contortus was identified and characterized using electron microscopy, spectrophotometry analyses, and biochemical approaches. The haemozoin was found to form in lipid droplets of intestinal cells, exhibiting regularly spherical structures with a 400-nm absorption peak. Furthermore, the formation of haemozoin in cultured larvae was influenced by culture time and the concentration of red blood cells added, and it could be inhibited by chloroquine-derived drugs.
PARASITES & VECTORS
(2023)
Article
Cell Biology
Hengzhi Shi, Xiaocui Huang, Xueqiu Chen, Yi Yang, Fei Wu, Chaoqun Yao, Guangxu Ma, Aifang Du
Summary: A secretory transthyretin-like protein, Hc-ttr-31, in H. contortus shows high transcription levels in the egg and early larval stages, with potential roles in regulating post-embryonic larval development and protecting gonads from oxidative stress. Knockdown of Hc-ttr-31 in C. elegans leads to shortened lifespan, decreased brood size, slowed pumping rate, and activation of apoptosis signalling pathway, indicating its importance in nematode development and reproduction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Christina D. Moon, Luis Carvalho, Michelle R. Kirk, Alan F. McCulloch, Sandra Kittelmann, Wayne Young, Peter H. Janssen, Dave M. Leathwick
Summary: Anthelmintic treatments, including a controlled release capsule (CRC) and a long-acting moxidectin injection (LAI), were found to significantly impact the rumen microbiota composition in adult ewes. The CRC treatment increased the relative abundances of specific archaeal species, while the LAI treatment led to minor changes in the bacterial and fungal communities. Overall, the anthelmintic treatments resulted in few but highly significant changes to the rumen microbiota composition.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Hai Xiang, Yi Fang, Zhiliang Tan, Rongzhen Zhong
Summary: The objective of this study was to investigate the relationship between gastrointestinal microbiota, protein metabolism, and Haemonchus contortus infection in lambs. The results showed that infection with H. contortus affected the digestive capacity, enzyme activity, and microbial diversity in the lambs. These findings provide insights into the consequences of H. contortus infection in lambs and could aid in the development of nutritional strategies to improve animal health.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Entomology
Xinxin Gao, Jihong Zhang, Qilian Qin, Peipei Wu, Huan Zhang, Qian Meng
Summary: Energy metabolism is crucial in insect metamorphosis, particularly in the larval-pupal stage of holometabolous insects. In this study, metabolome and transcriptome analyses were conducted to elucidate the metabolic changes and regulatory mechanisms during larval-pupal metamorphosis of the cotton bollworm, Helicoverpa armigera. The findings revealed that aerobic glycolysis supported cell proliferation and lipid synthesis during the feeding stage, while triglyceride degradation and acylcarnitine accumulation were activated during non-feeding stages. This study provides valuable insights into the metabolic regulation mechanism of lepidopteran larvae during their final stage of development.
Article
Cell Biology
Sachin Verma, David Crawford, Ali Khateb, Yongmei Feng, Eduard Sergienko, Gaurav Pathria, Chen-Ting Ma, Steven H. Olson, David Scott, Rabi Murad, Eytan Ruppin, Michael Jackson, Ze'ev A. Ronai
Summary: This study demonstrates the dependence of melanoma on the mitochondrial protein GCDH for cell survival and tumor growth. Inhibiting the GCDH pathway through NRF2 glutarylation leads to cell death and suppression of melanoma growth.
NATURE CELL BIOLOGY
(2022)
Review
Plant Sciences
Y. Liang, Y. Huang, C. Liu, K. Chen, M. Li
Summary: Lipids are essential for energy storage and membrane composition in plants. They also serve as substrates for the generation of signaling molecules. Despite their importance in stress response, the specific roles of plant lipids in abiotic stress remain largely unknown. This review provides a comprehensive overview of plant lipid metabolism and discusses their involvement in plant responses to abiotic stress, offering crucial background for further research on the interactions between plant lipids and stress.
Article
Oncology
Xueyu Wang, Mingo M. H. Yung, Rakesh Sharma, Fushun Chen, Ying-Tung Poon, Wai-Yip Lam, Benjamin Li, Hextan Y. S. Ngan, Karen K. L. Chan, David W. W. Chan
Summary: The study reveals that miR-33b is frequently silenced by DNA hypermethylation in metastatic ovarian cancer cells, and its restoration can impair lipid metabolic activities and reduce oncogenic properties, potentially serving as a therapeutic target for ovarian cancer metastatic progression.
Review
Chemistry, Multidisciplinary
Huocheng Yang, Xuemei Yao, Yingqi Liu, Xinkun Shen, Menghuan Li, Zhong Luo
Summary: Ferroptosis, a regulated cell death driven by iron-dependent phospholipid peroxidation, has gained attention in nanomedicine since 2012. It has unique features compared to other cell death modes and shows promise in treating resistant cancers. Recent insights suggest its connection to the tumor immune microenvironment, indicating its potential in stimulating antitumor immunity. Understanding the relationship between tumor metabolism and ferroptosis is important for developing antitumor therapies.
Article
Immunology
Haidong Wei, Luming Zhen, Shiquan Wang, Liufei Yang, Shuyue Zhang, Yuanyuan Zhang, Pengyu Jia, Tianyue Wang, Kui Wang, Yan Zhang, Lei Ma, Jianrui Lv, Pengbo Zhang
Summary: The study suggests that treatment with GTA can enhance lipid synthesis and IL-33 expression after ischemic stroke, thus improving blood-brain barrier repair and functional recovery.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Cell Biology
Ying Yang, Jiaxing He, Bo Zhang, Zhansheng Zhang, Guozhan Jia, Shiqi Liu, Tao Wu, Xianli He, Nan Wang
Summary: SLC25A1 is significantly upregulated in CRC, associated with poor survival in patients, and reprograms energy metabolism through two mechanisms to promote CRC growth. It serves as a potential diagnostic marker and therapeutic target in CRC.
CELL DEATH & DISEASE
(2021)
Article
Microbiology
Roz Laing, Stephen R. Doyle, Jennifer McIntyre, Kirsty Maitland, Alison Morrison, David J. Bartley, Ray Kaplan, Umer Chaudhry, Neil Sargison, Andy Tait, James A. Cotton, Collette Britton, Eileen Devaney
Summary: Through crossbreeding and gene expression analysis, we have identified certain genes associated with ivermectin resistance and proposed a novel mechanism that allows resistant worms to survive treatment. This is crucial for understanding ivermectin resistance and response to treatment in parasitic helminths.
Article
Pharmacology & Pharmacy
Jifei Miao, Xiaoming Liu, Yuanpin Liao, Yiwen Li, Yingyan Kuang, Juanxia Zheng, Zigang Li, Jiao Lan
Summary: This study investigates the roles of P53 and TREM2 in regulating macrophage lipid metabolism and phagocytic function. The findings reveal that P53 upregulates the expression of TREM2, promoting macrophage lipid metabolism, metabolic activity, and phagocytic capacity. The study also confirms the interaction between P53 and the TREM2 promoter.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Fisheries
Lijuan Yu, Juan Tian, Chenxi Zhang, Zhiyong Xue, Xing Lu, Hua Wen, Ming Jiang, Fan Wu
Summary: This study investigated the effects of different levels of acetylferulic paeonol ester (APE) on the growth performance, body composition, serum biochemical indicators, and lipid and glucose metabolism of Nile tilapia. The results showed that APE supplementation improved weight gain and decreased liver lipid content. It also affected serum biochemical parameters related to lipid and glucose metabolism. Transcriptome analysis revealed changes in gene expression related to fatty acid oxidation and degradation. APE influenced the hepatic glucose and lipid metabolism of tilapia at both enzymatic and mRNA levels.
Article
Biochemistry & Molecular Biology
Xindong Xu, Yifeng Wang, Changhong Wang, Gangqiang Guo, Xinyu Yu, Yang Dai, Yaobao Liu, Guiying Wei, Xiaohui He, Ge Jin, Ziqiu Zhang, Qingtian Guan, Arnab Pain, Shengyue Wang, Wenbao Zhang, Neil D. Young, Robin B. Gasser, Donald P. McManus, Jun Cao, Qi Zhou, Qingfeng Zhang
Summary: This study reports a chromosome-level reference genome for Schistosoma japonicum, shedding light on the evolution of sex chromosomes and providing insights into the sex determination and differentiation of this species. The study also identified W-linked genes and female-preferentially transcribed genes, which play critical roles in the maturation of female worms.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Ecology
Joshua A. Thia, Pasi K. Korhonen, Neil D. Young, Robin B. Gasser, Paul A. Umina, Qiong Yang, Owain Edwards, Tom Walsh, Ary A. Hoffmann
Summary: Genomic data provides insights into pest management, resistance evolution, pest invasions, and population dynamics. The first reference genome for the redlegged earth mite, Halotydeus destructor, was assembled to study pesticide adaptation and population demographics using whole-genome pool-seq data. Genetic mutations have been identified that contribute to organophosphate and pyrethroid resistance. Population genomic analyses reveal regional structuring in H. destructor populations, while demographic analyses indicate reduced genetic diversity and smaller effective population sizes in eastern populations. These findings have important implications for genetic markers, understanding resistance mechanisms, and predictive modeling of resistance evolution and spread.
JOURNAL OF EVOLUTIONARY BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Liina Kinkar, Pasi K. Korhonen, Urmas Saarma, Tao Wang, Xing-Quan Zhu, Ivon Harliwong, Bicheng Yang, J. Lynn Fink, Daxi Wang, Bill C. H. Chang, Galina N. Chelomina, Anson V. Koehler, Neil D. Young, Robin B. Gasser
Summary: Clonorchis sinensis shows significant genetic variation between populations in China and Far East Russia, which may have distinct epidemiology, pathogenicity, and chemotherapeutic responsiveness.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Biochemical Research Methods
Yan Liu, Guo Wei, Chen Li, Long-Chen Shen, Robin B. Gasser, Jiangning Song, Dijun Chen, Dong-Jun Yu
Summary: Single-cell RNA sequencing (scRNA-seq) has greatly facilitated the characterization of cell lineages and types in complex tissues and organisms. Cell-type annotation is important in scRNA-seq analysis, but manual annotation can be time-consuming and subjective. To address these challenges, we propose a new pipeline called TripletCell for cross-species, cross-protocol, and cross-sample cell-type annotation. Experimental results demonstrate that TripletCell outperforms existing methods and can accurately annotate different cell types regardless of protocols or species.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Biochemical Research Methods
Michelle Palmieri, Bruno Catimel, Dmitri Mouradov, Anuratha Sakthianandeswaren, Eugene Kapp, Ching-Seng Ang, Nicholas A. Williamson, Cameron J. Nowell, Michael Christie, Jayesh Desai, Peter Gibbs, Antony W. Burgess, Oliver M. Sieber
Summary: The nuclear translocation of PI3K alpha regulates the subcellular levels of PtdIns(3,4,5)P3 in colorectal cancer cell lines, and 867 potential nuclear PtdIns(3,4,5)P3 effector proteins have been identified. These effector proteins are mainly involved in RNA metabolism and may play a role in modulating pre-mRNA splicing. These findings suggest that nuclear PI3K alpha signaling through PtdIns(3,4,5)P3 effector proteins is important for CRC development.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemical Research Methods
S. Sadia Ameen, Nane Griem-Krey, Antoine Dufour, M. Iqbal Hossain, Ashfaqul Hoque, Sharelle Sturgeon, Harshal Nandurkar, Dominik F. Draxler, Robert L. Medcalf, Mohd Aizuddin Kamaruddin, Isabelle S. Lucet, Michael G. Leeming, Dazhi Liu, Amardeep Dhillon, Jet Phey Lim, Faiza Basheer, Hong-Jian Zhu, Laita Bokhari, Carli L. Roulston, Prasad N. Paradkar, Oded Kleifeld, Andrew N. Clarkson, Petrine Wellendorph, Giuseppe D. Ciccotosto, Nicholas A. Williamson, Ching-Seng Ang, Heung-Chin Cheng
Summary: Excitotoxicity is a neuronal death process initiated by over-stimulation of glutamate receptors. Proteolytic processing of proteins, particularly by calpains, plays a critical role in this process. Our study identified key synaptic regulatory proteins as substrates of calpains, and their proteolysis contributes to neuronal death by disrupting synaptic organization and function. Blocking calpain-mediated proteolysis of Src protein showed neuroprotective effects. Our findings provide new insights into excitotoxic neuronal death mechanisms and potential targets for neuroprotection.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Multidisciplinary Sciences
Ashish Sethi, Stephen M. Rawlinson, Abhinav Dubey, Ching-Seng Ang, Yoon Hee Choi, Fei Yan, Kazuma Okada, Ashley M. Rozario, Aaron M. Brice, Naoto Ito, Nicholas A. Williamson, Danny M. Hatters, Toby D. M. Bell, Haribabu Arthanari, Gregory W. Moseley, Paul R. Gooley
Summary: Viruses interact extensively with host proteins to modulate the infected cell's biology, often through multifunctional viral proteins. Traditional models cannot explain specific functions observed in certain viral proteins. In this study, we found that the unique phenotype of rabies virus P3 protein is determined by conformational arrangements rather than the presence of functional modules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Allergy
Thimo Ruethers, Elecia B. Johnston, Shaymaviswanathan Karnaneedi, Shuai Nie, Roni Nugraha, Aya C. Taki, Sandip D. Kamath, Nicholas A. Williamson, Sam S. Mehr, Dianne E. Campbell, Andreas L. Lopata
Article
Biology
Fuyi Li, Xudong Guo, Yue Bi, Runchang Jia, Miranda E. Pitt, Shirui Pan, Shuqin Li, Robin B. Gasser, Lachlan JM. Coin, Jiangning Song
Summary: A deep learning-based approach called Digerati was developed for the rapid and accurate identification of PE and PPE family proteins. Digerati achieved a significantly better performance (-18-20%) than alignment-based methods. It is expected to facilitate high-throughput identification and analysis of PE/PPE family members.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Tao Wang, Tiana F. Koukoulis, Laura J. Vella, Huaqi Su, Adityas Purnianto, Shuai Nie, Ching-Seng Ang, Guangxu Ma, Pasi K. Korhonen, Aya C. Taki, Nicholas A. Williamson, Gavin E. Reid, Robin B. Gasser
Summary: Many parasitic worms have a harmful impact on human and animal populations globally. Extracellular vesicles (EVs) have been found to play a role in modulating inflammatory/immune host responses and parasitism. In this study, EVs from the Haemonchus contortus parasite were enriched and a comprehensive analysis of EV proteins and lipids was conducted. The findings provide insights into the role of EV-specific molecules in parasite-host cross-talk and offer potential strategies to disrupt or eliminate parasite infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuanting Zheng, Neil D. Young, Jiangning Song, Robin B. Gasser
Summary: Biodiversity within the animal kingdom is associated with extensive molecular diversity. The expansion of genomic, transcriptomic, and proteomic data sets for invertebrate groups necessitates reliable in silico tools for molecular identification. This study established a sequence- and structure-based workflow system to identify and annotate proteases and protease inhibitors of a highly pathogenic parasitic roundworm. The results provide a solid foundation for further exploration of these molecules and their potential as drug targets. Rating: 8/10.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lucas G. Huggins, Vito Colella, Neil D. Young, Rebecca J. Traub
Summary: Apicomplexan haemoparasites cause significant morbidity and mortality, and traditional diagnostic methods have limitations. The nanopore sequencing method can accurately detect multiple pathogens, improving taxonomic resolution.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Chemistry, Multidisciplinary
Phuc H. H. Le, Denver P. P. Linklater, Arturo Aburto-Medina, Shuai Nie, Nicholas A. A. Williamson, Russell J. J. Crawford, Shane Maclaughlin, Elena P. P. Ivanova
Summary: This study developed a unique antifungal micro-pillared titanium surface pattern that inhibits biofilm formation by Candida albicans and multi-drug resistant Candida auris fungi. The micro-textured surface kills a significant proportion of Candida cells and prevents the proliferation of the remaining cells. This discovery provides new insights for designing durable antifungal surfaces to prevent biofilm formation by pathogenic, multi-drug resistant yeasts.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Biochemical Research Methods
Tong Pan, Chen Li, Yue Bi, Zhikang Wang, Robin B. Gasser, Anthony W. Purcell, Tatsuya Akutsu, Geoffrey Webb, Seiya Imoto, Jiangning Song
Summary: PFresGO is an attention-based deep-learning approach that incorporates hierarchical structures in Gene Ontology (GO) graphs and natural language processing algorithms for functional annotation of proteins, achieving superior performance compared to existing methods.