Article
Genetics & Heredity
Yurong Tai, Xue Yang, Deping Han, Zihan Xu, Ganxian Cai, Jiaqi Hao, Bingjie Zhang, Xuemei Deng
Summary: Egg production rate in chicken is related to the continuity of follicle development. High-yielding layer breeds show more active proliferation and differentiation of granulosa cells (GCs), and genes promoting GC proliferation and secretion of various sex hormones are upregulated in these breeds. Low-yielding variety exhibits upregulated signaling pathways that provide energy to GCs and promote angiogenesis.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Zonghao Tang, Renfeng Xu, Zhenghong Zhang, Congjian Shi, Yan Zhang, Hongqin Yang, Qingqiang Lin, Yiping Liu, Fengping Lin, Baorong Geng, Zhengchao Wang
Summary: Hypoxia drives the activation of HIF-1 alpha/BNIP3 signaling, inducing an increase in autophagy, which protects GCs from apoptosis during follicular development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Shunshun Han, Jianping Wang, Can Cui, Chunlin Yu, Yao Zhang, Diyan Li, Menggen Ma, Huarui Du, Xiaosong Jiang, Qing Zhu, Chaowu Yang, Huadong Yin
Summary: This study explores the regulatory molecular mechanisms of follicular atresia in chickens and reveals the role of autophagy and apoptosis in this process. It also highlights the importance of fibromodulin in regulating autophagy and provides insights into broodiness and poultry production improvement.
Article
Cell Biology
Feng Yang, Qiang Liu, Yanhong Chen, Huizhen Ye, Han Wang, Shenming Zeng
Summary: The study conducted quantitative proteomic and phosphoproteomic analyses on ovarian follicular atresia in porcine, identifying potential biomarkers and pathways involved in the process. The findings suggest novel explanations for the regulation of follicular atresia by analyzing dynamic changes in protein expression and phosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Shuo Zhou, An Zhao, Yangyang Wu, Yuling Mi, Caiqiao Zhang
Summary: Research has shown that autophagy is increased in aging hens compared to peak-laying hens, and treatment with grape seed proanthocyanidin (GSPs) can restore elevated autophagy and decrease viability of granulosa cells (GCs) affected by oxidative stress. GSPs inhibit autophagy in aging hens and promote antioxidant capacity, while also increasing GC survival through inhibition of the FoxO1 pathway and activation of the SIRT1 signal. This suggests a potential mechanism for GSPs in alleviating ovarian aging in poultry.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Ruixue Nie, Xiaotong Zheng, Wenhui Zhang, Bo Zhang, Yao Ling, Hao Zhang, Changxin Wu
Summary: This study identified key genes and regulatory pathways for follicle selection in chickens by measuring the histological characteristics, reproductive hormone concentration, and transcriptional profiles of follicles. The results provide deep insights into the crucial molecular mechanism of follicle development and egg-laying performance in chickens.
Article
Agriculture, Dairy & Animal Science
Leyan Yan, Mengdie Hu, Lihong Gu, Mingming Lei, Zhe Chen, Huanxi Zhu, Rong Chen
Summary: This study aimed to elucidate the molecular mechanisms underlying abnormal egg-laying in laying hens subjected to heat stress. The results showed that heat stress impaired laying performance in hens, causing damage to follicular granulosa cells and dysfunction in steroidogenesis.
Article
Biotechnology & Applied Microbiology
Jing Li, Su-Jin Si, Xing Wu, Zi-Hao Zhang, Chong Li, Yi-Qing Tao, Peng-Kun Yang, Dong-Hua Li, Zhuan-Jian Li, Guo-Xi Li, Xiao-Jun Liu, Ya-Dong Tian, Xiang-Tao Kang
Summary: In this study, it was found that circEML1 was differentially expressed in the ovarian tissues of chickens at different ages (15 W/20 W/30 W/ 68 W). The circEML1 was identified as a loop structure from EML1 pre-mRNA and promoted the expressions of CYP19A1/ StAR and E2/P4 secretion in GCs. Additionally, circEML1 acted as a sponge of gga-miR-449a and IGF2BP3 was targeted by gga-miR-449a to regulate steroidogenesis through the mTOR/p38MAPK pathway in GCs of chicken.
Article
Agriculture, Dairy & Animal Science
Kaue R. Martins, Cristina S. Haas, Monique T. Rovani, Fabiana Moreira, Andre L. F. Goetten, Juliana G. Ferst, Valerio M. Portela, Raj Duggavathi, Vilceu Bordignon, Paulo B. D. Goncalves, Bernardo G. Gasperin, Thomaz Lucia Jr
Summary: Leptin and its receptor are present in follicular cells of cattle ovaries, but it does not seem to play a crucial role in steroidogenesis and growth of dominant follicles.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Reproductive Biology
Zhengda Li, Ziyun Ruan, Yun Feng, Yanxin Wang, Jun Zhang, Canqiang Lu, Deshun Shi, Fenghua Lu
Summary: This study investigated the potential relationship between follicular atresia, granulosa cell autophagy, and N6-methyladenosine (m6A) modification. The findings revealed that autophagy in granulosa cells increased with the degree of follicle atresia, while the overall m6A level decreased. Rapamycin treatment induced atresia in cultured follicles, whereas 3-Methyladenine inhibited follicular atresia. The study also showed that METTL3 levels were lower and FTO levels were higher in progressed atretic follicle granulosa cells compared to healthy follicle granulosa cells. Overall, the results suggest that m6A modification can regulate autophagy in granulosa cells and play a role in the process of porcine follicular atresia.
Article
Biotechnology & Applied Microbiology
Heidi A. Van Every, Carl J. Schmidt
Summary: This study investigates the metabolic reprogramming in the liver between Day 4 and Day 20 post-hatch, revealing differences in metabolic pathways and important metabolites during different stages of metabolism. At Day 4, metabolic flexibility allows for rapid liver growth efficiency under oxygen-limiting conditions, while at Day 20, the liver's metabolism shifts to process a carbohydrate-rich diet that supports the rapid overall growth of modern broilers.
Article
Biochemistry & Molecular Biology
S. A. Masudul Hoque, Takashi Umehara, Tomoko Kawai, Masayuki Shimada
Summary: This study elucidated the negative effects of ROS on mitochondrial functions in FSH-stimulated granulosa cells during follicular development, and investigated the potential of PQQ treatment to ameliorate these effects. PQQ treatment reduced ROS levels and oxidative stress, improved cell viability and function, and increased the number of ovulated oocytes and pups per delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
G. D. Catandi, D. R. Bresnahan, S. O. Peters, K. J. Fresa, L. J. Maclellan, C. D. Broeckling, E. M. Carnevale
Summary: Many age-related changes in metabolite abundance suggest impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chenfeng Yuan, Zheng Li, Yun Zhao, Xin Wang, Lu Chen, Zijiao Zhao, Maosheng Cao, Tong Chen, Tariq Iqbal, Boqi Zhang, Wenjing Fan, Yameng Wei, Chunjin Li, Xu Zhou
Summary: This study demonstrated that exosomes play a crucial role in regulating the physiological function of granulosa cells by carrying specific microRNAs, promoting cell proliferation and progesterone synthesis. The MAPK/ERK and WNT/B-CATENIN pathways were found to be involved in these processes.
Article
Reproductive Biology
Manman Shen, Mingzhu Wang, Dehui Li, Yuan Feng, Liang Qu, Jinyu Wang
Summary: The study identified miRNAs associated with follicular development in chickens through small RNA sequencing of granulosa cells, and analyzed their target genes and their relationships with signaling pathways, providing basic data and a new theoretical foundation for further research on the role of miRNAs in follicular development in chickens.
Article
Cell Biology
Chunheng Mo, Guoqing Cai, Long Huang, Qiuyang Deng, Dongliang Lin, Lin Cui, Yajun Wang, Juan Li
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2015)
Article
Genetics & Heredity
Lin Cui, Can Lv, Jiannan Zhang, Chunheng Mo, Dongliang Lin, Juan Li, Yajun Wang
Article
Endocrinology & Metabolism
Chunheng Mo, Long Huang, Lin Cui, Can Lv, Dongliang Lin, Liang Song, Guoqiang Zhu, Juan Li, Yajun Wang
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2017)
Article
Endocrinology & Metabolism
Haikun Liu, Qin Luo, Jiannan Zhang, Chunheng Mo, Yajun Wang, Juan Li
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2019)
Article
Physiology
Chunheng Mo, Can Lv, Long Huang, Zhengyang Li, Jiannan Zhang, Juan Li, Yajun Wang
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Multidisciplinary Sciences
Guoqiang Zhu, Chao Fang, Chunheng Mo, Yajun Wang, Yan Huang, Juan Li
Summary: The transcriptome analysis of Gp and Gd cells in chicken preovulatory follicles revealed differences in cell cycle regulation, steroidogenesis, extracellular matrix remodeling, cell adhesion, sperm binding, and signaling pathways, which are likely controlled by gonadotropins and local ovarian factors such as GD-derived factors.
SCIENTIFIC REPORTS
(2021)
Article
Physiology
Jiannan Zhang, Can Lv, Chunheng Mo, Meng Liu, Yiping Wan, Juan Li, Yajun Wang
Summary: This study analyzed the transcriptome of each cell population in adult chicken anterior pituitaries using single-cell RNA sequencing technology and identified four out of five known endocrine cell clusters along with four non-endocrine/secretory cell types. The research found that gonadotrophs abundantly express five peptide hormones and folliculo-stellate cells may act as a paracrine/autocrine signaling center. Sexually dimorphic expression of many genes were also identified in most cell clusters, providing a comprehensive understanding of vertebrate pituitary biology.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Junfeng Chen, Huijuan Bi, Mats E. Pettersson, Daiki X. Sato, Angela P. Fuentes-Pardo, Chunheng Mo, Shady Younis, Ola Wallerman, Patric Jern, Gregorio Moles, Ana Gomez, Gunnar Kleinau, Patrick Scheerer, Leif Andersson
Summary: This study reveals that TSHR alleles associated with reproductive timing in Atlantic herring show differences in cAMP signaling between spring-spawning and autumn-spawning individuals, with both coding and non-coding variants in the TSHR locus potentially regulating seasonal reproduction.
COMMUNICATIONS BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Meng Liu, Guixian Bu, Yiping Wan, Jiannan Zhang, Chunheng Mo, Juan Li, Yajun Wang
Summary: This study reveals for the first time the physiological function of neuropeptide W as a CRIF in chickens, inhibiting the synthesis and secretion of ACTH to suppress the activity of the stress axis.
Article
Immunology
Dengcheng Zhou, Jing Zhang, Chengju Xiao, Chunheng Mo, Bi-Sen Ding
Summary: This study found that the gut microbial metabolite TMAO can reduce the progression of liver fibrosis in a mouse NASH model. Further analysis revealed that TMAO normalizes gut microbiota diversity and preserves liver sinusoidal endothelial cell integrity by inhibiting endothelial beta 1-subunit of Na(+), K(+)-ATPase (ATP1B1) expression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Yue Deng, Xingming Huang, Yan Hu, Weiting Zhong, Hua Zhang, Chunheng Mo, Hongjun Wang, Bi-Sen Ding, Chen Wang
Summary: Vascular leakage and inflammation are pathological hallmarks of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Endothelial FGFR1 signaling loss leads to increased ROCK2 activity, resulting in inflammation and vascular leakage in vivo and in vitro. Inhibition of ROCK2 by TDI01 provides potential therapeutic value in the treatment of ALI/ARDS.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell Biology
Jinfeng Xu, Fan Zhou, Xiaodong Wang, Chunheng Mo
Summary: Ferroptosis is a regulated form of cell death characterized by iron overload, lipid peroxidation, and antioxidant system disruption. Recent evidence suggests that it is associated with pregnancy related diseases such as spontaneous abortion, pre-eclampsia, gestational diabetes mellitus, intrahepatic cholestasis of pregnancy, and spontaneous preterm birth. Inhibiting ferroptosis may offer a potential treatment option for these disorders. This review summarizes the mechanisms and advancements in ferroptosis, its pathogenic role in pregnancy related diseases, and potential therapeutic interventions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Yong Qiu, Chunheng Mo, Shiyu Xu, Lu Chen, Wanlin Ye, Yi Kang, Guo Chen, Tao Zhu
Summary: The blood-brain barrier (BBB) is a crucial barrier that separates the central nervous system from the periphery, consisting of endothelial cells, pericytes, astrocytes, synapses, and tight junction proteins. During the perioperative period, anesthesia and surgical procedures pose stress on the body, leading to potential blood-brain barrier damage and brain metabolism dysfunction. The destruction of the blood-brain barrier during the perioperative period is closely associated with cognitive impairment and increased risk of postoperative mortality, hindering the enhancement of recovery after surgery. The specific pathophysiological processes and mechanisms underlying blood-brain barrier damage during the perioperative period have not been fully elucidated, but changes in blood-brain barrier permeability, inflammation and neuroinflammation, oxidative stress, ferroptosis, and intestinal dysbiosis may be involved. This review aims to summarize the research progress on perioperative blood-brain barrier damage, its potential adverse effects, and molecular mechanisms to provide insights for the study of brain function homeostasis and precision anesthesia.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mengli Yan, Hui Li, Shiyu Xu, Jinyan Wu, Jiachen Li, Chengju Xiao, Chunheng Mo, Bi-Sen Ding
Summary: Chronic liver diseases affect a large number of people worldwide and often lead to fibrosis. Nonalcoholic steatohepatitis (NASH) is a disease characterized by liver fibrosis, and its causes and treatments are not well understood. In this study, the researchers investigated the role of endothelial necroptosis in NASH progression using a mouse model. They found that knockout of the MLKL gene specifically in endothelial cells reduced liver fibrosis in the NASH model. This suggests that targeting endothelial MLKL could be a promising strategy for developing treatments for NASH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
