Article
Agriculture, Dairy & Animal Science
Xiaoxu Shen, Xiyu Zhao, Haorong He, Yao Zhang, Qing Zhu, Huadong Yin
Summary: SLC5A5 plays a crucial role in the development and function of chicken ovarian follicle granulosa cells, influencing cell proliferation, steroid hormone synthesis, and apoptosis. This discovery sheds light on the broader context of chicken ovarian follicle development and potential ways to improve egg-laying performance through molecular marker-assisted breeding technology.
Article
Agriculture, Dairy & Animal Science
Conghao Zhong, Zhansheng Liu, Dandan Li, Li Kang, Yunliang Jiang
Summary: Follicle selection is a crucial process for chicken laying performance and fecundity. In this study, the impacts of follicle-stimulating hormone (FSH) on chicken granulosa cells were investigated through ONT transcriptome sequencing. FSH treatment upregulated the expression of several genes related to steroid biosynthesis and pathways associated with ovarian steroidogenesis. Specifically, TRAF7 was identified as a key regulator in promoting the expression of steroidogenic enzymes and granulosa cell proliferation. This study provides valuable insights into the molecular mechanism of follicle selection in chickens.
Article
Genetics & Heredity
Dandan Li, Conghao Zhong, Yi Sun, Li Kang, Yunliang Jiang
Summary: By comparing the transcriptomes of granulosa cells between pre-hierarchical and hierarchical follicles in chickens, genes underlying follicle selection were identified. Differential gene, transcript, and lncRNA expression were observed between pre-hierarchical and hierarchical follicles. This study provides important insights into the genetic mechanisms underlying follicle selection in chickens and other poultry species.
FRONTIERS IN GENETICS
(2023)
Article
Agriculture, Dairy & Animal Science
Jie Liu, Shudi Dai, Zichun Dai, Yuyan Feng, Mingming Lei, Rong Chen, Huanxi Zhu
Summary: High-throughput sequencing technology was used to analyze the mRNAs, miRNAs, and lncRNAs that play a critical role in follicular selection, and a ceRNA regulatory network was constructed. Significant changes in extracellular matrix, cell junction, and glucose and lipid metabolism were confirmed during follicular selection. Five miRNAs, including miR-222-3p, miR-2954-3p, miR-126-5p, miR-2478, and miR-425-5p, were identified as potentially important regulatory molecules in goose follicle selection.
Article
Agriculture, Dairy & Animal Science
Manman Shen, Tao Li, Yuan Feng, Ping Wu, Boris Ramos Serrano, Adileidys Ruiz Barcenas, Liang Qu, Weiguo Zhao
Summary: This study found that the flavonoid quercetin has a regulatory effect on granulosa cells (GCs) in the prehierarchical follicles of chickens. Specifically, at appropriate concentrations, quercetin can promote cell proliferation and progesterone secretion, which is beneficial for follicle development and selection.
Article
Multidisciplinary Sciences
Diyan Li, Chunyou Ning, Jiaman Zhang, Yujie Wang, Qianzi Tang, Hua Kui, Tao Wang, Mengnan He, Long Jin, Jing Li, Yu Lin, Bo Zeng, Huadong Yin, Xiaoling Zhao, Yao Zhang, Huailiang Xu, Qing Zhu, Mingzhou Li
Summary: This study investigates the transcriptomic and chromatin architecture dynamics of chicken granulosa cells during folliculogenesis and reveals the consistent reprogramming of chromatin architecture and transcriptomic divergence. The results provide insights into compartmentalization rearrangement, variable organization of topologically associating domains, and rewiring of long-range promoter-enhancer interaction in granulosa cells.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Laurie Francoeur, Claire S. Stephens, Patricia A. Johnson
Summary: Intense selective breeding of broiler breeds of chickens has led to decreased egg production in broiler breeder hens, and limiting feed intake can improve egg production but may impact reproductive efficiency.
Article
Agriculture, Dairy & Animal Science
Ying Wang, Zhenyu Guo, Chen Zi, Pengfei Wu, Xiaoyang Lv, Lan Chen, Fuxiang Chen, Genxi Zhang, Jinyu Wang
Summary: In this study, it was found that circRNA expression differed in small yellow follicles of Jinghai Yellow hens under red light and white light conditions, with some differentially expressed circRNAs interacting with genes related to ovarian steroidogenesis and signaling pathways, potentially influencing granulosa cell differentiation and follicular development.
Article
Agriculture, Dairy & Animal Science
Shunshun Han, Jianping Wang, Can Cui, Chunlin Yu, Yao Zhang, Diyan Li, Menggen Ma, Huarui Du, Xiaosong Jiang, Qing Zhu, Chaowu Yang, Huadong Yin
Summary: This study explores the regulatory molecular mechanisms of follicular atresia in chickens and reveals the role of autophagy and apoptosis in this process. It also highlights the importance of fibromodulin in regulating autophagy and provides insights into broodiness and poultry production improvement.
Article
Reproductive Biology
Yanqin Gong, Dandan Li, Yi Sun, Li Kang, Yunliang Jiang
Summary: This study reveals the important role of Noggin4 in chicken follicle development, particularly in the proliferation of granulosa cells.
Article
Reproductive Biology
Manman Shen, Mingzhu Wang, Dehui Li, Yuan Feng, Liang Qu, Jinyu Wang
Summary: The study identified miRNAs associated with follicular development in chickens through small RNA sequencing of granulosa cells, and analyzed their target genes and their relationships with signaling pathways, providing basic data and a new theoretical foundation for further research on the role of miRNAs in follicular development in chickens.
Article
Cell Biology
Yuanyuan Hou, Jianing Hu, Jie Li, Hu Li, Yangqing Lu, Xingting Liu
Summary: The study aimed to investigate the role of MFN2 in the proliferation and progesterone biosynthesis of granulosa cells (GCs) during follicle selection in hens. The results showed that the expression level of MFN2 in GCs increased significantly after follicle selection, and it promoted P4 biosynthesis, ATP production, mtDNA copy numbers, and cell proliferation. FSH treatment enhanced these effects in F5G, but not in SYFG. Additionally, overexpression of MFN2 increased P4 biosynthesis, ATP production, mtDNA copy numbers, and cell proliferation in SYFG. These findings suggest that MFN2 upregulation may play a crucial role in the initiation of P4 biosynthesis and the promotion of GCs proliferation during follicle selection.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Reproductive Biology
Sen Li, Lei-Ning Chen, Hai-Jing Zhu, Xie Feng, Feng-Yun Xie, Shi-Ming Luo, Xiang-Hong Ou, Jun-Yu Ma
Summary: A total of 6883 high variable genes were found in 4888 ovarian follicular somatic cells. The analysis revealed metabolic reprogramming and different gene expression patterns in the follicular somatic cells.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Kurumi Fukuda, Masafumi Muraoka, Yuzuru Kato, Yumiko Saga
Summary: The gene expression profile of pre-granulosa cells in mice undergoes significant changes during primordial follicle formation, with oocyte development playing a crucial role in this process.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biotechnology & Applied Microbiology
Yanhong Zhang, Qiuyue Chen, Yuanyuan Guo, Li Kang, Yi Sun, Yunliang Jiang
Summary: Follicle selection in chickens is a crucial process controlled by sex hormones, which impacts egg production. In this study, the phosphoproteomes of small yellow (SY) and F6 follicles were compared, leading to the identification of differentially phosphorylated proteins. The expression of LSD1Ser54p, a phosphorylated protein, was found to be upregulated in F6 follicles, indicating its involvement in follicle selection.
Article
Multidisciplinary Sciences
Xiaotong Zheng, Bo Zhang, Yawen Zhang, Haian Zhong, Ruixue Nie, Junying Li, Hao Zhang, Changxin Wu
SCIENTIFIC REPORTS
(2020)
Article
Genetics & Heredity
Lulu Wang, Gang Zheng, Yiming Yuan, Ziyi Wang, Changjun Liu, Hao Zhang, Ling Lian
Summary: RNA-Seq analysis of different spleens in Marek's disease revealed that the tumorous spleens group had more splice site region variants, leading to more AS events. Functional enrichment analysis showed that pathways related to MD were significantly enriched.
Article
Agriculture, Dairy & Animal Science
Weihua Tian, Xin Hao, Ruixue Nie, Yao Ling, Bo Zhang, Hao Zhang, Changxin Wu
Summary: Long non-coding RNAs (lncRNAs) play crucial regulatory roles in chicken adipogenesis by modulating lipid-related pathways through cis- and trans-regulatory mechanisms, as well as competing with miRNA to regulate lipid-related protein-encoding genes. Identification of key lncRNAs provides insights into the functional mechanisms underlying adipogenesis in chickens and contributes to genetic improvements targeting excessive abdominal fat.
Article
Agriculture, Dairy & Animal Science
Peng Shang, Bo Zhang, Pan Li, Zulfiqar Ahmed, Xiaoxiang Hu, Yangzom Chamba, Hao Zhang
Summary: By using dual expression omics, this study identified key candidate genes involved in high-altitude hypoxia adaptation and revealed their roles in mitochondrial function, blood particle regulation, and glycolysis.
Article
Multidisciplinary Sciences
Yawen Zhang, Bo Zhang, Ying Zhang, Ruixue Nie, Jian Zhang, Peng Shang, Hao Zhang
Summary: In this study, a high-quality chromosome-level genome of the bar-headed goose was sequenced, assembled, and analyzed, providing insights into the genetic basis of adaptation to life at a high altitude.
Article
Agriculture, Dairy & Animal Science
Xiaotong Zheng, Ziwei Chen, Wuchao Zhuang, Jilong Zhang, Jiaheng He, Yinku Xie, Jianfei Chen
Summary: This study investigated the expression profiles of genes related to feed intake in broiler chicks in different energy states. The expression levels of LEAP2 in the liver and small intestine varied significantly with changes in diet, and CDX4 may be a potential regulator of LEAP2 expression in the small intestine. The study highlights the importance of LEAP2 for avian feed intake.
Article
Agriculture, Dairy & Animal Science
Xiaotong Zheng, Yinku Xie, Ziwei Chen, Jiaheng He, Jianfei Chen
Summary: This study investigated the effects of glycine supplementation in drinking water on the growth performance and intestinal development of newborn chicks. The findings showed that glycine supplementation increased the average daily gain of chicks and promoted the development of intestinal villi. It also influenced the expression of genes related to mucosal immune barrier, cellular antioxidant capacity, and amino acid absorption in the jejunum. These results suggest that glycine supplementation in drinking water can enhance growth efficiency and promote intestinal health in chicks.