Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Review
Engineering, Biomedical
Nikolas Di Caprio, Jason A. Burdick
Summary: Cellular spheroids are being explored as building blocks for tissue engineering and as tissue models. Biomaterials are being used to guide spheroid formation and function, and also facilitate the fabrication of tissue constructs. This review provides an overview of recent studies on the role of engineered biomaterials in supporting spheroids in biomedical applications.
ACTA BIOMATERIALIA
(2023)
Article
Nanoscience & Nanotechnology
Shabir Hassan, Eduardo Gomez-Reyes, Eduardo Enciso-Martinez, Kun Shi, Jorge Gonzalez Campos, Oscar Yael Perez Soria, Eder Luna-Ceron, Myung Chul Lee, Isaac Garcia-Reyes, Joshua Steakelum, Haziq Jeelani, Luis Enrique Garcia-Rivera, Minsung Cho, Stephanie Sanchez Cortes, Tom Kamperman, Haihang Wang, Jeroen Leijten, Lance Fiondella, Su Ryon Shin
Summary: A combinational multimaterial and embedded bioprinting approach is used to develop implantable constructs that can maintain their structure in vivo. Self-healing and biodegradable colloidal gels are introduced as support baths to improve printing fidelity and provide suitable microenvironments for cell growth and infiltration.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Shangjing Xin, Kaivalya A. Deo, Jing Dai, Navaneeth Krishna Rajeeva Pandian, David Chimene, Robert M. Moebius, Abhishek Jain, Arum Han, Akhilesh K. Gaharwar, Daniel L. Alge
Summary: An in-depth investigation was conducted on the dissipation process of HMPs during printing, revealing that external resistance and internal physicochemical properties play a key role in the printing outcome. Understanding these mechanisms can help improve the printability of HMPs and enhance their use in 3D bioprinting.
Article
Engineering, Biomedical
Margaret E. Prendergast, Su-Jin Heo, Robert L. Mauck, Jason A. Burdick
Summary: Due to the limited healing capacity of the meniscus, there is a clinical need for the development of constructs that replicate meniscal tissue organization for better repair. Suspension bath bioprinting technology allows for the fabrication of complex structures using non-viscous bioinks. In this study, anisotropic constructs with embedded hydrogel fibers were printed using suspension bath printing and showed improved cell and collagen alignment, as well as enhanced tensile moduli compared to constructs without fibers. This work advances biofabrication for meniscal tissue repair.
Article
Engineering, Biomedical
Margaret E. Prendergast, Matthew D. Davidson, Jason A. Burdick
Summary: The extracellular matrix contains biochemical and biophysical cues that regulate cell behavior and mechanical properties. Methods to align hydrogel fibers within cell-degradable bioink filaments have been developed, allowing for directional cell spreading and tissue engineering applications. Fabrication techniques such as extrusion printing and bioink parameters influence the alignment of fibers and resulting cell behavior, advancing the development of physiological tissue models and tissue repair strategies.
Article
Engineering, Biomedical
Hyeongjin Lee, Carlos Kengla, Han Su Kim, Ickhee Kim, Jae-Gu Cho, Eric Renteria, Kyungsup Shin, Anthony Atala, James J. Yoo, Sang Jin Lee
Summary: Medical imaging and 3D bioprinting can be used to create patient-specific bone scaffolds with complex shapes and controlled inner architectures. This study investigates the effectiveness of a biomimetic approach to scaffold design and demonstrates that the biomimetic scaffold with a dense external layer improves bone regeneration compared to the control scaffold. Histological examination confirms the superior bone regeneration in the biomimetic scaffolds and suggests that patient-specific anatomy can be accurately translated into a 3D bioprinting strategy, leading to clinically relevant constructs.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Nadezhda V. Arguchinskaya, Elena V. Isaeva, Anastas A. Kisel, Evgeny E. Beketov, Tatiana S. Lagoda, Denis S. Baranovskii, Nina D. Yakovleva, Grigory A. Demyashkin, Liudmila N. Komarova, Svetlana O. Astakhina, Nikolai E. Shubin, Peter V. Shegay, Sergey A. Ivanov, Andrey D. Kaprin
Summary: Gelatin methacryloyl (GelMA) has attracted attention due to its tunable mechanical properties. This study synthesized highly substituted GelMA and investigated its suitability for 3D printing, cytocompatibility, and biocompatibility. The results showed that a 15% concentration GelMA achieved satisfactory printing quality, but the high degree of substitution decreased the adhesion ability of human adipose-derived stem cells (ADSCs) to the GelMA surface. In animal implantation, GelMA degradation was slowed down.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Zongzhe Xuan, Leonid Gurevich, Jesper de Claville Christiansen, Vladimir Zachar, Cristian Pablo Pennisi
Summary: In this study, the effects of cyclic mechanical stimulation on smooth muscle cell maturation were investigated using three-dimensional constructs. The results showed that methacrylate-modified surfaces supported stable adhesion of the 3D constructs and facilitated cyclic mechanical stimulation, resulting in increased expression of contractile markers. These effects were mediated by activation of the p38 MAPK pathway. These findings provide valuable insights into the role of mechanical signaling in maintaining the contractile phenotype of bladder smooth muscle cells and have important implications for future treatments of LUT diseases.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Annan Chen, Wanying Wang, Zhengyi Mao, Yunhu He, Shiting Chen, Guo Liu, Jin Su, Pei Feng, Yusheng Shi, Chunze Yan, Jian Lu
Summary: Additive manufacturing (AM) has significant advantages in the fabrication of tissue engineering implants. However, many tissue constructs have anisotropic heterogeneous structures, which pose challenges for conventional AM processes. Multimaterial 3D and 4D bioprinting offer a promising solution for constructing multifunctional implants with heterogeneous structures that can better mimic the host microenvironment.
ADVANCED MATERIALS
(2023)
Article
Medicine, Research & Experimental
WonJin Kim, Chul Ho Jang, GeunHyung Kim
Summary: This study introduces a new strategy of using hybrid structures composed of EC spheroids and hASCs for bone regeneration, which demonstrated outstanding angiogenic and osteogenic activities both in vitro and in vivo.
Article
Polymer Science
Catherine G. Y. Ngan, Anita Quigley, Richard J. Williams, Cathal D. O'Connell, Romane Blanchard, Mitchell Boyd-Moss, Tim D. Aumann, Penny McKelvie, Gordon G. Wallace, Peter F. M. Choong, Rob M. I. Kapsa
Summary: This study developed a biofabrication technique to engineer muscle for research and clinical applications. It demonstrated myoblast migration through a bioprinted GelMA scaffold, showing cells spontaneously forming fibers on the material surface, leading to advanced maturation of muscle tissue. This methodology has wide applications for in vitro and in vivo neuromuscular function and disease modeling.
Article
Chemistry, Multidisciplinary
Kazim K. Moncal, Hemanth Gudapati, Kevin P. Godzik, Dong N. Heo, Youngnam Kang, Elias Rizk, Dino J. Ravnic, Hwabok Wee, David F. Pepley, Veli Ozbolat, Gregory S. Lewis, Jason Z. Moore, Ryan R. Driskell, Thomas D. Samson, Ibrahim T. Ozbolat
Summary: The study demonstrates the intra-operative bioprinting of various craniomaxillofacial tissues directly on rats, including bone, skin, and composite tissues. A novel osteogenic hard tissue ink and droplet-based bioprinting technology facilitated substantial bone regeneration and accelerated wound closure in a stratified arrangement. The use of a hybrid approach resulted in significant skin wound closure and bone coverage, showcasing the potential for bioprinting technologies in clinical CMF defect reconstruction.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
X. B. Chen, A. Fazel Anvari-Yazdi, X. Duan, A. Zimmerling, R. Gharraei, N. K. Sharma, S. Sweilem, L. Ning
Summary: Bioinks combine biomaterials and living cells, and are used in extrusion bioprinting to create 3D structures that resemble human tissue or organs. These printed constructs have various applications in tissue engineering and in vitro tissue modeling. The properties of the bioinks, including rheological, mechanical, and biological properties, as well as the printing process, play a crucial role in successful printing and application. This article critically reviews the latest developments in bioinks and biomaterial solutions for extrusion bioprinting, emphasizing bioink synthesis, characterization, and their influence on the printing process.
BIOACTIVE MATERIALS
(2023)
Review
Polymer Science
Matangi Parimala Chelvi Ratnamani, Xinping Zhang, Hongjun Wang
Summary: Regenerative medicine has experienced exponential growth in the past few decades, evolving from complete tissue removal to fabricating engineered tissues using personalized living cells and customized matrices. Biofabrication through 3D printing enables precise and controlled assemblies of cells and biomaterials, mimicking the heterogenous microenvironment of living tissue. This review provides a concise framework for the bio-manufacturing process and explores the versatile applications of hydrogels in bioprinting and tissue formation.
Review
Biotechnology & Applied Microbiology
Jaryl Chen Koon Ng, Daniel Wee Yee Toong, Valerie Ow, Su Yin Chaw, Hanwei Toh, Philip En Hou Wong, Subbu Venkatraman, Tze Tec Chong, Lay Poh Tan, Ying Ying Huang, Hui Ying Ang
Summary: Drug delivery systems in cardiovascular applications include drug-eluting stents and drug-coated balloons to ensure effective treatment. Besides antiproliferative drugs, growth factors, genetic materials, hormones, and signaling molecules are also used. Nanoencapsulation techniques are developed for targeted drug delivery. This review discusses drug delivery and coating mechanisms in current drug-eluting stents and drug-coated balloons, as well as innovations in drug-eluting stent technologies and drug encapsulation in nanocarriers for vascular diseases.
Article
Engineering, Manufacturing
Xuhui Zhang, Zhen Xiao, Wenhui Yu, Chee Kai Chua, Lihua Zhu, Zongshen Wang, Peng Xue, Shuai Tan, Yongling Wu, Hongyu Zheng
Summary: The study on Er-modified 7075 aluminum alloy showed that the addition of Er can reduce crack density but increase porosity, with Er existing in the form of unmelted particles and sub-micrometer scale Al3Er. The deterioration of fluidity in the molten pool due to Er particles leads to higher porosity, while the coherency between aluminum matrix and Al3Er favors grain refinement and crack reduction.
VIRTUAL AND PHYSICAL PROTOTYPING
(2022)
Review
Materials Science, Multidisciplinary
Hong Wei Tan, Yu Ying Clarrisa Choong, Che Nan Kuo, Hong Yee Low, Chee Kai Chua
Summary: In recent years, the 3D printed electronics technology has attracted significant attention from industries and researchers due to its unique features. This review provides an in-depth overview of the latest 3D electronic printing techniques, innovative practical technologies, and upcoming application trends in advanced functional materials for fabricating 3D printed electronics devices.
PROGRESS IN MATERIALS SCIENCE
(2022)
Article
Oncology
Choi-Fong Cho, Charlotte E. Farquhar, Colin M. Fadzen, Benjamin Scott, Pei Zhuang, Niklas von Spreckelsen, Andrei Loas, Nina Hartrampf, Bradley L. Pentelute, Sean E. Lawler
Summary: This study describes the use of a tumor-targeting peptide that can penetrate the blood-brain barrier and target glioblastoma (GBM). The peptide was conjugated with an insoluble anti-cancer drug, improving solubility and retaining efficacy against GBM stem cells. The study demonstrates enhanced blood-brain barrier permeability and therapeutic targeting to GBM, leading to higher toxicity in GBM cells compared to normal brain tissues and prolonged survival in animal models. This research offers a new approach for targeted therapy in GBM.
Article
Engineering, Manufacturing
Che Nan Kuo, Yu Ping Wang, Chee Kai Chua
Summary: Metal additive manufacturing technologies have been widely used in aerospace and medical device industries. Porous materials have been extensively studied due to the maturity of such technologies. This study explores the relationship between the mechanical properties of porous materials and factors like surface roughness and ratio between surface roughness and strut diameter. It also investigates the formulation development of an electrolyte with a surfactant to enhance the surface finish during the electropolishing process of 3D printed Ti-6Al-4V porous materials.
VIRTUAL AND PHYSICAL PROTOTYPING
(2022)
Article
Chemistry, Applied
Yi Zhang, Amelia Yilin Lee, Kanitthamniyom Pojchanun, Cheng Pau Lee, Aiwu Zhou, Jia An, Michinao Hashimoto, U-Xuan Tan, Chen Huei Leo, Gladys Wong, Chee Kai Chua, Aakanksha Pant
Summary: Three-dimensional food printing can help consumers accept and consume alternative proteins more easily by printing final products that resemble familiar natural foods in terms of taste, texture, and appearance. This study focuses on optimizing multicomponent food inks fortified with alternative proteins for 3D printing using response surface methodology (RSM). The addition of xanthan gum as a shear thinning and hydrating agent optimized the printability and syneresis of the six protein inks. This RSM-based approach has the potential to be widely adopted in 3D food printing.
FOOD HYDROCOLLOIDS
(2022)
Article
Biochemistry & Molecular Biology
Chen Huei Leo, Su Yi Foo, Joseph Choon Wee Tan, U-Xuan Tan, Chee Kai Chua, Eng Shi Ong
Summary: This study aims to extract bioactive compounds from orange peel waste (OPW) using a green extraction method called pressurized hot water extraction (PHWE). The results showed that extraction temperatures of 60 degrees C and 80 degrees C yielded distinct bioactive compounds with better antioxidant capacity. Specifically, treatment with 60 degrees C OPW extracts inhibited vascular inflammation and endothelial dysfunction in vitro, suggesting its vasoprotective effects mediated by anti-inflammatory effects.
Review
Engineering, Biomedical
Jessica Mancilla-De-la-Cruz, Marisela Rodriguez-Salvador, Jia An, Chee Kai Chua
Summary: This paper presents the use of 3D printing technology in drug delivery applications. The different variations of the technique according to characteristics, materials, and dosage forms are discussed. The seven main categories of 3D printing and the most frequent materials used are introduced. The paper also explores different forms of drug dosage and provides insights for those interested in the advancement of drug delivery and the development of 3D printing applications in this area.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Engineering, Biomedical
Amedeo Franco Bonatti, Giovanni Vozzi, Chee Kai Chua, Carmelo De Maria
Summary: This paper proposes a deep learning-based control loop for extrusion-based bioprinting that automatically optimizes printing parameters and monitors the printing process online. By collecting a comprehensive dataset and training a machine learning model, the authors demonstrate the automation of quality control in the bioprinting process.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Engineering, Manufacturing
Aakanksha Pant, Phoebe Xin Ni Leam, Chee Kai Chua, U-Xuan Tan
Summary: Food waste utilisation and zero waste approach are effective ways to promote a sustainable economy. Three-dimensional food printing (3DFP) can help turn food waste into value-added products that resemble familiar consumables. In this study, spinach stems and kale stalks were used to formulate food inks that were tested for their rheological properties and suitability for dysphagia patients. The research demonstrates a promising method of converting vegetable waste into aesthetically pleasing edible diets through 3DFP.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Article
Engineering, Manufacturing
Cherq Chua, Swee Leong Sing, C. K. Chua
Summary: This study uses finite element analysis to investigate the mechanical performance of in-situ alloyed titanium-tantalum lattice structures fabricated using laser powder bed fusion. The study finds that both simulation methods accurately predict the elastic modulus, but underestimate the yield strength. Additionally, the study shows that the performance of uniform lattice structures agrees with the Gibson-Ashby model, and suggests that more information on defect conditions is needed to improve simulation accuracy.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Article
Computer Science, Artificial Intelligence
Xi Huang, Wei Long Ng, Wai Yee Yeong
Summary: In this study, a machine learning approach was used to predict the number of cells within printed droplets based on droplet velocity at two different points along the nozzle-substrate distance. A contactless method combining an optical system and machine learning algorithms was employed for cell detection within single droplets and prediction of the total number of printed cells within multiple droplets. Various machine learning algorithms were compared, and the random forest regressor algorithm achieved the highest accuracy in cell prediction within single droplets and the extra tree regressor algorithm demonstrated the lowest mean error in predicting the number of printed cells within multiple droplets. Incorporating these models in a droplet monitoring system enables live assessment of the number of printed cells during an inkjet-based bioprinting process.
JOURNAL OF INTELLIGENT MANUFACTURING
(2023)
Article
Engineering, Biomedical
Wei Long Ng, Xi Huang, Viktor Shkolnikov, Ratima Suntornnond, Wai Yee Yeong
Summary: This study investigates the influence of bioink properties on printing performance and cell health, finding that higher bioink viscoelasticity helps to stabilize droplet filaments and higher bioink viscosity improves droplet deposition accuracy and cell viability.
BIO-DESIGN AND MANUFACTURING
(2023)
Article
Materials Science, Biomaterials
Ratima Suntornnond, Wei Long Ng, Xi Huang, Chuen Herh Ethan Yeow, Wai Yee Yeong
Summary: Material jetting bioprinting is a promising technique for 3D bioprinting, allowing precise and accurate deposition of biomaterials and cells at predetermined positions. One major challenge is the limited range of printable hydrogel-based bio-inks. In this study, gelatin methacrylate (GelMA) was modified to improve its printability in a thermal inkjet bioprinter. The modification process involved saponification and heat treatment to obtain saponified GelMA (SP-GelMA) and heat-treated SP-GelMA (HSP-GelMA). The modified bio-inks demonstrated good printability and biocompatibility, making them suitable for cell-cell and cell-material interaction studies.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)