Article
Biochemistry & Molecular Biology
Sergey Okhota, Sergey Kozlov, Yuliya Avtaeva, Ivan Melnikov, Olga Saburova, Konstantin Guria, Evgeny Matroze, Zufar Gabbasov
Summary: This study examined platelet adhesion mediated by von Willebrand factor (VWF) at high shear rates in patients with premature coronary artery disease (CAD). The researchers found that patients with premature CAD exhibited a greater GPIb-mediated component of platelet adhesion compared to control patients. This suggests a potential role of excessive VWF-platelet interactions in the development of premature CAD.
Article
Hematology
Justine H. Ryu, Kenneth A. Bauer, Sol Schulman
Summary: This report describes the successful perioperative management of a patient with type 2N VWD using the recombinant FVIII product efanesoctocog alfa. By decoupling the FVIII-VWF interaction, efanesoctocog alfa achieves prolonged FVIII circulation and mitigates potential cardiovascular risks.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Marcela Montilla, Isabel Atienza-Navarro, Francisco Jose Garcia-Cozar, Carmen Castro, Francisco Javier Rodriguez-Martorell, Felix A. Ruiz
Summary: Polyphosphate (polyP) plays an important modulatory role in the binding of von Willebrand factor (VWF) and Factor VIII (FVIII) in blood coagulation, and may be used in developing new therapies to activate VWF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Maria Teresa Pagliari, Frits R. Rosendaal, Minoo Ahmadinejad, Zahra Badiee, Mohammad-Reza Baghaipour, Luciano Baronciani, Olga Benitez Hidalgo, Imre Bodo, Ulrich Budde, Giancarlo Castaman, Peyman Eshghi, Jenny Goudemand, Mehran Karimi, Bijan Keikhaei, Riitta Lassila, Frank W. G. Leebeek, Maria Fernanda Lopez Fernandez, Pier Mannuccio Mannucci, Renato Marino, Johannes Oldenburg, Ian Peake, Cristina Santoro, Reinhard Schneppenheim, Andreas Tiede, Gholamreza Toogeh, Alberto Tosetto, Marc Trossaert, Hamideh Yadegari, Eva M. K. Zetterberg, Flora Peyvandi, Augusto B. Federici, Jeroen Eikenboom
Summary: The VWFpp/VWF:Ag ratio indicates missense variants, while the FVIII:C/VWF:Ag ratio does not discriminate between missense and null alleles. The level of VWFpp is not associated with the severity of bleeding symptoms.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Mechanics
Christian J. Spieker, Gabor Zavodszky, Clarisse Mouriaux, Pierre H. Mangin, Alfons G. Hoekstra
Summary: A combined in silico and in vitro platform is created to analyze flow conditions and cellular behavior in the onset of a hemostatic response to a microneedle-induced vessel puncture. The platform uses a cell-resolved blood flow model for detailed flow profile and cell distribution analyses, and a novel punctured vessel flow chamber to evaluate platelet aggregation around the wound neck. The simulations and experiments provide insight into platelet transport and aggregation processes in the wound area.
Article
Hematology
Maria Teresa Pagliari, Ulrich Budde, Luciano Baronciani, Peyman Eshghi, Minoo Ahmadinejad, Zahra Badiee, Mohammad-Reza Baghaipour, Olga Benitez Hidalgo, Eugenia Biguzzi, Imre Bodo, Giancarlo Castaman, Jenny Goudemand, Mehran Karimi, Bijan Keikhaei, Riitta Lassila, Frank W. G. Leebeek, Maria Fernanda Lopez Fernandez, Renato Marino, Johannes Oldenburg, Ian Peake, Cristina Santoro, Reinhard Schneppenheim, Andreas Tiede, Gholamreza Toogeh, Alberto Tosetto, Marc Trossaert, Hamideh Yadegari, Eva M. K. Zetterberg, Pier Mannuccio Mannucci, Augusto B. Federici, Jeroen Eikenboom, Flora Peyvandi
Summary: The prevalence of anti-von Willebrand factor (VWF) antibodies was assessed in patients with type 3 von Willebrand disease (VWD). The study found that 8.4% of the subjects tested positive for anti-VWF antibodies, while 6% had neutralizing VWF inhibitors. These inhibitors were mainly found in patients homozygous for VWF null alleles.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Hematology
Omid Seidizadeh, Flora Peyvandi, Pier Mannuccio Mannucci
Summary: Von Willebrand disease is caused by quantitative or qualitative defects of von Willebrand factor (VWF). Type 2N VWD is a rare recessive disorder that requires accurate diagnosis and differentiation from other bleeding disorders such as mild/moderate hemophilia A.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Stephen J. X. Murphy, Soon Tjin Lim, Fionnuala Hickey, Justin A. Kinsella, Deirdre R. Smith, Sean Tierney, Bridget Egan, T. Martin Feeley, Sinead M. Murphy, D. Ronan Collins, Tara Coughlan, Desmond O'Neill, Joseph A. Harbison, Prakash Madhavan, Sean M. O'Neill, Mary-Paula Colgan, James S. O'Donnell, Jamie M. O'Sullivan, George Hamilton, Dominick J. H. McCabe
Summary: The study found that VWF:Ag levels were higher and VWFpp/VWF:Ag ratios were lower in early symptomatic patients with carotid stenosis compared to asymptomatic patients, and these levels decreased over time. Additionally, there was a difference in VWF:Ag levels between patients with blood group O and those without blood group O.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biophysics
Aleksey V. Belyaev, Yulia K. Kushchenko
Summary: Platelet adhesion and activation are crucial initial processes in arterial and microvascular hemostasis, hindered by high bloodflow forces. von Willebrand factor (VWF), a blood plasma protein, plays a key role in the mechanochemical regulation of shear-induced platelet aggregation (SIPA). Recent studies have shown that mechanical tension on the extracellular part of glycoprotein receptor GPIb can trigger biochemical reactions in platelets, leading to integrin alpha IIb beta 3 activation and strengthened adhesion. Computer simulations of platelet adhesion to surface-grafted VWF multimers in pressure-driven flow show that GPIb-mediated mechanotransduction is a feasible way of platelet activation and aggregation under high shear stress. A quantitative understanding of mechanochemical processes involved in SIPA could lead to the discovery of new anti-platelet medication and the development of numerical models for platelet thrombosis and hemostasis.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zohaib Akram, Sultan Aati, Hein Ngo, Amr Fawzy
Summary: The study synthesized and characterized chlorhexidine-loaded mesoporous silica nanoparticles grafted with poly-L-glycolic acid, and tested their in vitro drug release, cytotoxicity, and antimicrobial capacity in different pH environments. The experimental nanoparticles demonstrated low cytotoxicity and high antibacterial efficacy, showing potential for use in adhesive and restorative dentistry.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Hematology
Masayuki Kubo, Kazuya Sakai, Masaki Hayakawa, Hirokazu Kashiwagi, Hideo Yagi, Yoshinobu Seki, Atsushi Hasegawa, Haruyuki Tanaka, Itsuto Amano, Yoshiaki Tomiyama, Masanori Matsumoto
Summary: This study investigated whether VWF degradation is enhanced in patients with ET. The results showed that patients with higher platelet counts had lower HMW-VWFM index and higher VWF-DP:VWF Ag ratio. Cytoreductive therapy increased the HMW-VWFM index and decreased the VWF-DP:VWF Ag ratio. In conclusion, an increased platelet count in ET patients is associated with enhanced cleavage of VWF, and cytoreductive therapy can reduce this cleavage and improve VWFM distributions.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Michelle Lavin, Analia Sanchez Luceros, Peter Kouides, Rezan Abdul-Kadir, James S. O'Donnell, Ross Baker, Maha Othman, Sandra L. Haberichter
Summary: The management of pregnant women with von Willebrand disease (VWD) varies internationally, especially in antenatal, peripartum, and postpartum management. There is uncertainty surrounding the use of neuraxial anesthesia (NA) for women with type 2 and type 3 VWD, highlighting the need for international collaborative research efforts to improve care for pregnant women with VWD.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Nanoscience & Nanotechnology
Kai Yue, Chao Yang, Yu You, Xueying Wang, Xinxin Zhang
Summary: In this study, an artificial blood vessel system was constructed to investigate the adhesion behaviors of nanocarriers in microvessels. The results confirmed that the gelatin-based artificial vessel showed good cytocompatibility and mechanical properties for studying nanoparticle adhesion characteristics. Temperature variation had a minimal impact on nanoparticle adhesion, and designing ligands resistant to temperature changes can enhance drug retention.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Hematology
Eugenia Biguzzi, Simona Maria Siboni, Saskia le Cessie, Luciano Baronciani, Frits R. Rosendaal, Astrid van Hylckama Vlieg, Flora Peyvandi
Summary: The study evaluated the differential increase of VWF and FVIII:C with age among different ages and types of VWD.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Michelle Lavin, Pamela Christopherson, Julie Grabell, Thomas Abshire, Veronica Flood, Sandra L. Haberichter, David Lillicrap, James S. O'Donnell, Robert R. Montgomery, Paula D. James
Summary: This study evaluates the clinical utility of an interim bleeding protocol in VWD patients and provides a new approach to longitudinal bleeding assessment and insights into the evolution of bleeding in VWD.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Nanoscience & Nanotechnology
Moran Levi, Mark Epshtein, Tatsiana Castor, Meinrad Gawaz, Netanel Korin
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2020)
Article
Biophysics
Yathreb Asaad, Mark Epshtein, Netanel Korin
Summary: This study presents an innovative approach to investigate particle adhesion in a converging artery-sized model, demonstrating the impact of particle size, suspension buffer, and surrounding environment on the adhesion distribution of drug carriers. The findings provide insights for optimizing intravascular drug carriers and their targeting properties.
JOURNAL OF BIOMECHANICS
(2021)
Article
Multidisciplinary Sciences
Maria Khoury, Mark Epshtein, Netanel Korin
Summary: This paper introduces a new method of fabricating real-sized, reconstructed human arterial models using 3D printing technology, lining them with human endothelial cells (ECs), and studying particle targeting under physiological flow. The technique may serve as a new platform for studying drug targeting in the cardiovascular system and improving the design of injectable nanomedicines, offering significant tools for studying targeted delivery of different agents for cardiovascular diseases under patient-specific flow and physiological conditions.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Chemistry, Multidisciplinary
Ariel A. Szklanny, Majd Machour, Idan Redenski, Vaclav Chochola, Idit Goldfracht, Ben Kaplan, Mark Epshtein, Haneen Simaan Yameen, Uri Merdler, Adam Feinberg, Dror Seliktar, Netanel Korin, Josef Jaros, Shulamit Levenberg
Summary: Creating engineered hierarchical vasculatures is crucial for implantable functional thick tissues. This study introduces a combined approach using millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues to achieve fully engineered hierarchical vascular constructs for implantation. The use of sacrificial molds, endothelial cells, and vascularizing hydrogels enables the formation of functional vessels within the engineered tissue, promoting tissue perfusion and host vasculature ingrowth.
ADVANCED MATERIALS
(2021)
Article
Multidisciplinary Sciences
Ximing Li, Luna Rizik, Valeriia Kravchik, Maria Khoury, Netanel Korin, Ramez Daniel
Summary: Complex biological systems involve cells acting collectively to solve sophisticated tasks, while researchers have implemented neural network-like computing in bacterial consortia for pattern recognition.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Mark Epshtein, Moran Levi, Afif M. Kraitem, Hikaia Zidan, Robert M. King, Meinrad Gawaz, Matthew J. Gounis, Netanel Korin
Summary: Localized delivery of diagnostic/therapeutic agents to cerebral aneurysms and brain arterial lesions through targeted particulate carriers shows potential for a new treatment paradigm. By leveraging the endogenous biophysical properties of brain aneurysms and utilizing flow guided targeting with functionalized microparticles, the study demonstrates enhanced localization at high-risk aneurysms both in vitro and in vivo. This biophysical strategy may offer novel treatment opportunities for cerebral aneurysms.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Rossana Rauti, Adi Ess, Baptiste Le Roi, Yevgeniy Kreinin, Mark Epshtein, Netanel Korin, Ben M. Maoz
Summary: The Insert-Chip microfluidic device offers the functionalities of an Organ-on-a-Chip platform in a user-friendly and reusable manner, allowing for co-culturing of cells, exposure to flow, and observation of interactions. This novel device overcomes measurement and imaging challenges associated with standard Organ-on-a-Chip platforms, demonstrating its promise for investigating biological functions, cell-cell interactions, and response to therapeutics.
APL BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Ramsha Aamir, Cameron Fyffe, Netanel Korin, Daniel A. Lawrence, Enming J. Su, Mathumai Kanapathipillai
Summary: This study reports a novel nanoformulation of plasmin based on heparin and arginine, which shows increased stability and efficacy. In vitro studies demonstrate significant plasmin stability and efficient fibrinolytic activity with the nanoformulation. The research indicates the potential of using plasmin nanoparticles in ischemic stroke therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jeremy A. Nestele, Anne-Katrin Rohlfing, Valerie Dicenta, Alexander Bild, Daniela Eissler, Frederic Emschermann, Marcel Kremser, Konstantin Krutzke, Tilman E. Schaeffer, Oliver Borst, Moran Levi, Netanel Korin, Meinrad Paul Gawaz
Summary: Nanoparticles provide a novel approach for targeted antithrombotic therapy, delivering drugs to specific sites with low side effects. In this study, nanoparticles combined with established proteins successfully inhibited platelet aggregation and thrombus formation, indicating a promising therapeutic strategy for antithrombotic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neuroimaging
Vania Anagnostakou, Mark Epshtein, Giovanni J. Ughi, Robert M. King, Antonios Valavanis, Ajit S. Puri, Matthew J. Gounis
Summary: Transvascular imaging with high-frequency optical coherence tomography (HF-OCT) was used to study the micro-architectonics of the subarachnoid space (SAS). Different configurations and porosity of subarachnoid trabeculae (SAT) in various locations were analyzed, showing a predilection for specific arachnoid morphologies. The study demonstrated the feasibility of imaging SAS structures and paved the way for further understanding their function and impact on neurovascular pathologies.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2022)
Article
Engineering, Biomedical
Barak Zohar, Lior Debbi, Majd Machour, Netta Nachum, Idan Redenski, Mark Epshtein, Netanel Korin, Shulamit Levenberg
Summary: Vascularization of 3D engineered tissues is a challenge in tissue engineering. This study introduces the design and fabrication of an AngioTube, a biodegradable macro-vessel surrounded by micro-channel array, to support physiological flow distribution and integration with living capillaries. The engineered micro-channels guide endothelial cells to form patent micro-vessels, and in-vitro and in-vivo models demonstrate anastomosis with vascular networks. This approach offers a new micro-fabrication strategy with high geometrical accuracy at the tissue-scale level.
ACTA BIOMATERIALIA
(2023)
Article
Neuroimaging
Charles Skarbek, Vania Anagnostakou, Emanuele Procopio, Mark Epshtein, Christopher M. Raskett, Romeo Romagnoli, Giorgio Iviglia, Marco Morra, Marta Antonucci, Antonino Nicoletti, Giuseppina Caligiuri, Matthew J. Gounis
Summary: The study suggests that coating clot retrieval devices with DNA-binding compounds can significantly improve the outcome of mechanical thrombectomy procedures in stroke patients.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Article
Neuroimaging
Robert M. King, Ahmet Peker, Mark Epshtein, Jennifer M. Arends, Alice B. Brochu, Christopher M. Raskett, Kimiko J. Slazas, Ajit S. Puri, Adam S. Arthur, David Fiorella, Matthew J. Gounis, Vania Anagnostakou
Summary: This study tested the effectiveness of a heparin-coated flow diverter in reducing the risk of thromboembolic complications. The results showed that the coated device significantly reduced the number of new magnetic susceptibility artifacts after 1 week follow-up, indicating its potential in reducing thromboembolic complications.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Article
Neuroimaging
Christopher T. Zoppo, Mark Epshtein, Matthew J. Gounis, Vania Anagnostakou, Robert M. King
Summary: Flow diversion is a common treatment for cerebral aneurysms, but it has drawbacks such as the need for dual antiplatelet therapy and delayed complete occlusion of the aneurysm. Biomimetic surface modifications have been developed to reduce thrombogenicity, but there are concerns about delayed endothelialization. In this study, the in vivo performance of flow diverters with different surface modifications was evaluated in rabbit models.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)