State‐of‐the‐art CRISPR /Cas9 Technology for Genome Editing in Trypanosomatids

Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi

(2019) Yuko Takagi et al.   PLoS Neglected Tropical Diseases

Functional analysis and importance for host cell infection of the Ca2+-conducting subunits of the mitochondrial calcium uniporter of Trypanosoma cruzi

(2019) Miguel A. Chiurillo et al.   MOLECULAR BIOLOGY OF THE CELL

CRISPR in Parasitology: Not Exactly Cut and Dried!

(2019) Jessica M. Bryant et al.   TRENDS IN PARASITOLOGY

MICU1 and MICU2 Play an Essential Role in Mitochondrial Ca2+ Uptake, Growth, and Infectivity of the Human Pathogen Trypanosoma cruzi

(2019) Mayara S. Bertolini et al.   mBio

Gene Disruption of Honey Bee Trypanosomatid Parasite, Lotmaria passim, by CRISPR/Cas9 System

(2019) Qiushi Liu et al.   Frontiers in Cellular and Infection Microbiology

Improvements in the CRISPR/Cas9 system for high efficiency gene disruption in Trypanosoma cruzi

(2018) Bruno A.A. Romagnoli et al.   ACTA TROPICA

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

Assessment of two CRISPR-Cas9 genome editing protocols for rapid generation of Trypanosoma cruzi gene knockout mutants

(2018) Gabriela Assis Burle-Caldas et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

A Riboswitch-based Inducible Gene Expression System for Trypanosoma brucei

(2018) Teresa Cruz-Bustos et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High-Performance Probes

(2018) Teresa Cruz-Bustos et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Exploiting CRISPR–Cas9 technology to investigate individual histone modifications

(2018) Juan-José Vasquez et al.   NUCLEIC ACIDS RESEARCH

New and emerging uses of CRISPR/Cas9 to genetically manipulate apicomplexan parasites

(2018) Manlio Di Cristina et al.   PARASITOLOGY

Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes

(2018) Eva Rico et al.   Scientific Reports

Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping

(2018) Fernanda Cristina Costa et al.   PLoS Neglected Tropical Diseases

Genetically Validated Drug Targets in Leishmania: Current Knowledge and Future Prospects

(2018) Nathaniel G. Jones et al.   ACS Infectious Diseases

An Intracellular Ammonium Transporter Is Necessary for Replication, Differentiation, and Resistance to Starvation and Osmotic Stress in Trypanosoma cruzi

(2018) Teresa Cruz-Bustos et al.   mSphere

Calcium-sensitive pyruvate dehydrogenase phosphatase is required for energy metabolism, growth, differentiation, and infectivity of Trypanosoma cruzi

(2018) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Advances in the application of genetic manipulation methods to apicomplexan parasites

(2017) C.E. Suarez et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Recent advances in Leishmania reverse genetics: Manipulating a manipulative parasite

(2017) Samuel M. Duncan et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

(2017) Alexandra East-Seletsky et al.   MOLECULAR CELL

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

Different Roles of Mitochondrial Calcium Uniporter Complex Subunits in Growth and Infectivity of Trypanosoma cruzi

(2017) Miguel A. Chiurillo et al.   mBio

Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins

(2017) Lia Carolina Soares Medeiros et al.   mBio

Prevalence of Chagas Disease in a U.S. Population of Latin American Immigrants with Conduction Abnormalities on Electrocardiogram

(2017) Mahmoud I. Traina et al.   PLoS Neglected Tropical Diseases

Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit

(2017) Daniel Martel et al.   Biomed Research International

A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

(2017) Tom Beneke et al.   Royal Society Open Science

Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms

(2017) Wen-Wei Zhang et al.   mSphere

The Heroes of CRISPR

(2016) Eric S. Lander   CELL

Safety Profile of Nifurtimox for Treatment of Chagas Disease in the United States

(2016) Colin J. Forsyth et al.   CLINICAL INFECTIOUS DISEASES

CRISPR/Cas9-mediated endogenous C-terminal Tagging ofTrypanosoma cruziGenes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor

(2016) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Genome Editing by CRISPR/Cas9: A Game Change in the Genetic Manipulation of Protists

(2016) Noelia Lander et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

EuPathDB: the eukaryotic pathogen genomics database resource

(2016) Cristina Aurrecoechea et al.   NUCLEIC ACIDS RESEARCH

Subtelomeric I-SceI-Mediated Double-Strand Breaks Are Repaired by Homologous Recombination in Trypanosoma cruzi

(2016) Miguel A. Chiurillo et al.   Frontiers in Microbiology

First efficient CRISPR-Cas9-mediated genome editing inLeishmaniaparasites

(2015) Lauriane Sollelis et al.   CELLULAR MICROBIOLOGY

Expanding the tool box for genetic manipulation of Trypanosoma cruzi

(2015) Gabriela de Assis Burle-Caldas et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

25 years of African trypanosome research: From description to molecular dissection and new drug discovery

(2015) Keith R. Matthews   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

TcPho91 is a contractile vacuole phosphate sodium symporter that regulates phosphate and polyphosphate metabolism inTrypanosoma cruzi

(2015) Veronica Jimenez et al.   MOLECULAR MICROBIOLOGY

CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani

(2015) Wen-Wei Zhang et al.   mBio

CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment

(2015) Noelia Lander et al.   mBio

Molecular parasitology in the 21st Century

(2015) Roberto Docampo   Essays in Biochemistry

Neglected Parasitic Infections in the United States: Chagas Disease

(2014) Susan P. Montgomery et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

Probing druggability and biological function of essential proteins inLeishmaniacombining facilitated null mutant and plasmid shuffle analyses

(2014) Mariko Dacher et al.   MOLECULAR MICROBIOLOGY

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

(2014) Mehdi Ghorbal et al.   NATURE BIOTECHNOLOGY

Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9

(2014) Saima M. Sidik et al.   PLoS One

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9

(2014) B. Shen et al.   mBio

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

RNA Interference in Protozoan Parasites: Achievements and Challenges

(2011) Nikolay G. Kolev et al.   EUKARYOTIC CELL

Hyperosmotic Stress Induces Aquaporin-dependent Cell Shrinkage, Polyphosphate Synthesis, Amino Acid Accumulation, and Global Gene Expression Changes inTrypanosoma cruzi

(2011) Zhu-Hong Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Microhomology-mediated deletion and gene conversion in African trypanosomes

(2010) Lucy Glover et al.   NUCLEIC ACIDS RESEARCH

Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes

(2008) L. Glover et al.   NUCLEIC ACIDS RESEARCH