Production of CAR T-cells by GMP-grade lentiviral vectors: Latest advances and future prospects

Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T Cell Responses

(2018) Jang Hwan Cho et al.   CELL

A guide to manufacturing CAR T cell therapies

(2018) Philipp Vormittag et al.   CURRENT OPINION IN BIOTECHNOLOGY

The expansion of targetable biomarkers for CAR T cell therapy

(2018) Michelle H. Townsend et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

Grading of cytokine release syndrome associated with the CAR T cell therapy tisagenlecleucel

(2018) David Porter et al.   Journal of Hematology & Oncology

PI3K orchestration of the in vivo persistence of chimeric antigen receptor-modified T cells

(2018) Wenting Zheng et al.   LEUKEMIA

Modeling cytokine release syndrome

(2018) Cliona Rooney et al.   NATURE MEDICINE

Monocyte-derived IL-1 and IL-6 are differentially required for cytokine-release syndrome and neurotoxicity due to CAR T cells

(2018) Margherita Norelli et al.   NATURE MEDICINE

CAR T cell–induced cytokine release syndrome is mediated by macrophages and abated by IL-1 blockade

(2018) Theodoros Giavridis et al.   NATURE MEDICINE

Immunotherapy: Cancer immunotherapy and the value of cure

(2018) Joseph C. Del Paggio   Nature Reviews Clinical Oncology

Chimeric Antigen Receptor Therapy

(2018) Carl H. June et al.   NEW ENGLAND JOURNAL OF MEDICINE

CAR-T cells: the long and winding road to solid tumors

(2018) Maria Michela D’Aloia et al.   Cell Death & Disease

Reducing Ex Vivo Culture Improves the Anti-leukemic Activity of Chimeric Antigen Receptor (CAR)-T Cells

(2018) Saba Ghassemi et al.   Cancer Immunology Research

Targeted delivery of a PD-1-blocking scFv by CAR-T cells enhances anti-tumor efficacy in vivo

(2018) Sarwish Rafiq et al.   NATURE BIOTECHNOLOGY

The biological basis and clinical symptoms of CAR-T therapy-associated toxicites

(2018) Aleksei Titov et al.   Cell Death & Disease

Immune Cell Hacking: Challenges and Clinical Approaches to Create Smarter Generations of Chimeric Antigen Receptor T Cells

(2018) Reza Elahi et al.   Frontiers in Immunology

Allogeneic CAR-T Cells: More than Ease of Access?

(2018) Charlotte Graham et al.   Cells

Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor

(2017) A J Valkama et al.   GENE THERAPY

Integrated Method for Purification and Single-Particle Characterization of Lentiviral Vector Systems by Size Exclusion Chromatography and Tunable Resistive Pulse Sensing

(2017) Susanne Heider et al.   MOLECULAR BIOTECHNOLOGY

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

(2017) Justin Eyquem et al.   NATURE

Chimeric Antigen Receptor T Cells in Refractory B-Cell Lymphomas

(2017) Stephen J. Schuster et al.   NEW ENGLAND JOURNAL OF MEDICINE

TCR engagement negatively affects CD8 but not CD4 CAR T cell expansion and leukemic clearance

(2017) Yinmeng Yang et al.   Science Translational Medicine

Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment

(2017) Oladapo O. Yeku et al.   Scientific Reports

Advancing chimeric antigen receptor T cell therapy with CRISPR/Cas9

(2017) Jiangtao Ren et al.   Protein & Cell

Bioengineering Solutions for Manufacturing Challenges in CAR T Cells

(2017) Nicole J. Piscopo et al.   Biotechnology Journal

mSA2 affinity-enhanced biotin-binding CAR T cells for universal tumor targeting

(2017) Jason J. Lohmueller et al.   OncoImmunology

Global Manufacturing of CAR T Cell Therapy

(2017) Bruce L. Levine et al.   Molecular Therapy-Methods & Clinical Development

Regional Delivery of Chimeric Antigen Receptor (CAR) T-Cells for Cancer Therapy

(2017) et al.   Cancers

Armored CAR T-cells: utilizing cytokines and pro-inflammatory ligands to enhance CAR T-cell anti-tumour efficacy

(2016) Oladapo O. Yeku et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

T cells expressing an anti-B-cell maturation antigen chimeric antigen receptor cause remissions of multiple myeloma

(2016) S. A. Ali et al.   BLOOD

Driving gene-engineered T cell immunotherapy of cancer

(2016) Laura A Johnson et al.   CELL RESEARCH

Vectors and strategies for nonviral cancer gene therapy

(2016) Jessica Pahle et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Antigen-specific T cells fully conserve antitumour function following cryopreservation

(2016) Jorge L Galeano Niño et al.   IMMUNOLOGY AND CELL BIOLOGY

Prospects to improve chimeric antigen receptor T-cell therapy for solid tumors

(2016) Chuan Jin et al.   Immunotherapy

Challenges of up-scaling lentivirus production and processing

(2016) Alexandra McCarron et al.   JOURNAL OF BIOTECHNOLOGY

BET bromodomain inhibition enhances T cell persistence and function in adoptive immunotherapy models

(2016) Yuki Kagoya et al.   JOURNAL OF CLINICAL INVESTIGATION

CD19 CAR–T cells of defined CD4+:CD8+ composition in adult B cell ALL patients

(2016) Cameron J. Turtle et al.   JOURNAL OF CLINICAL INVESTIGATION

Genome-wide Profiling Reveals Remarkable Parallels Between Insertion Site Selection Properties of the MLV Retrovirus and the piggyBac Transposon in Primary Human CD4+ T Cells

(2016) Andreas Gogol-Döring et al.   MOLECULAR THERAPY

Metabolic pathways in T cell activation and lineage differentiation

(2016) Luís Almeida et al.   SEMINARS IN IMMUNOLOGY

T Cells Expressing CD19/CD20 Bispecific Chimeric Antigen Receptors Prevent Antigen Escape by Malignant B Cells

(2016) E. Zah et al.   Cancer Immunology Research

Production of lentiviral vectors

(2016) Otto-Wilhelm Merten et al.   Molecular Therapy-Methods & Clinical Development

Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

(2016) Michele E Murphy et al.   Molecular Therapy-Methods & Clinical Development

Chimeric antigen receptor T-cell therapy for solid tumors

(2016) Kheng Newick et al.   Molecular Therapy-Oncolytics

Clinical manufacturing of CAR T cells: foundation of a promising therapy

(2016) Xiuyan Wang et al.   Molecular Therapy-Oncolytics

Comparison of Membrane Chromatography and Monolith Chromatography for Lactoferrin and Bovine Serum Albumin Separation

(2016) Chalore Teepakorn et al.   Processes

Performance-enhancing drugs: design and production of redirected chimeric antigen receptor (CAR) T cells

(2015) B L Levine   CANCER GENE THERAPY

Manufacture of T cells using the Sleeping Beauty system to enforce expression of a CD19-specific chimeric antigen receptor

(2015) H Singh et al.   CANCER GENE THERAPY

Manufacture of tumor- and virus-specific T lymphocytes for adoptive cell therapies

(2015) X Wang et al.   CANCER GENE THERAPY

A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteins

(2015) M P Marino et al.   GENE THERAPY

Progress and challenges in viral vector manufacturing

(2015) Johannes C.M. van der Loo et al.   HUMAN MOLECULAR GENETICS

Lentiviral Protein Transfer Vectors Are an Efficient Vaccine Platform and Induce a Strong Antigen-Specific Cytotoxic T Cell Response

(2015) Katharina M. Uhlig et al.   JOURNAL OF VIROLOGY

Lentivirus-mediated Gene Transfer in Hematopoietic Stem Cells Is Impaired in SHIV-infected, ART-treated Nonhuman Primates

(2015) Patrick M Younan et al.   MOLECULAR THERAPY

The pharmacology of second-generation chimeric antigen receptors

(2015) Sjoukje J. C. van der Stegen et al.   NATURE REVIEWS DRUG DISCOVERY

Remote control of therapeutic T cells through a small molecule-gated chimeric receptor

(2015) C.-Y. Wu et al.   SCIENCE

Chimeric antigen receptor T cells persist and induce sustained remissions in relapsed refractory chronic lymphocytic leukemia

(2015) David L. Porter et al.   Science Translational Medicine

An optimized method for high-titer lentivirus preparations without ultracentrifugation

(2015) Wei Jiang et al.   Scientific Reports

Downstream processing and chromatography based analytical methods for production of vaccines, gene therapy vectors, and bacteriophages

(2015) Petra Kramberger et al.   Human Vaccines & Immunotherapeutics

Tolerance and efficacy of autologous or donor-derived T cells expressing CD19 chimeric antigen receptors in adult B-ALL with extramedullary leukemia

(2015) Hanren Dai et al.   OncoImmunology

Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor

(2015) Jonathan Sheu et al.   Molecular Therapy-Methods & Clinical Development

Enhancement of the in vivo persistence and antitumor efficacy of CD19 chimeric antigen receptor T cells through the delivery of modified TERT mRNA

(2015) Yun Bai et al.   Cell Discovery

Evaluation of Intracellular Signaling Downstream Chimeric Antigen Receptors

(2015) Hannah Karlsson et al.   PLoS One

Construction of stable packaging cell lines for clinical lentiviral vector production

(2015) Khaled S. Sanber et al.   Scientific Reports

A highly compact epitope-based marker/suicide gene for easier and safer T-cell therapy

(2014) B. Philip et al.   BLOOD

Current concepts in the diagnosis and management of cytokine release syndrome

(2014) D. W. Lee et al.   BLOOD

Managing Cytokine Release Syndrome Associated With Novel T Cell-Engaging Therapies

(2014) Shannon L. Maude et al.   CANCER JOURNAL

Challenges in Manufacturing Adenoviral Vectors for Global Vaccine Product Deployment

(2014) Jort Vellinga et al.   HUMAN GENE THERAPY

Vector Integration and Tumorigenesis

(2014) Christof von Kalle et al.   HUMAN GENE THERAPY

Chimeric Antigen Receptor T Cells for Sustained Remissions in Leukemia

(2014) Shannon L. Maude et al.   NEW ENGLAND JOURNAL OF MEDICINE

Advances in cell culture: anchorage dependence

(2014) O.-W. Merten   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Efficacy and Toxicity Management of 19-28z CAR T Cell Therapy in B Cell Acute Lymphoblastic Leukemia

(2014) M. L. Davila et al.   Science Translational Medicine

Optimization of lentiviral vector production using polyethylenimine-mediated transfection

(2014) YONG TANG et al.   Oncology Letters

Simplified production and concentration of lentiviral vectors to achieve high transduction in primary human T cells

(2013) Adam P Cribbs et al.   BMC BIOTECHNOLOGY

piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice

(2013) R. Li et al.   Disease Models & Mechanisms

New developments in lentiviral vector design, production and purification

(2013) Maria Mercedes Segura et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

A new approach to gene therapy usingSleeping Beautyto genetically modify clinical-grade T cells to target CD19

(2013) Harjeet Singh et al.   IMMUNOLOGICAL REVIEWS

An immunologist's guide to CD31 function in T-cells

(2013) F. M. Marelli-Berg et al.   JOURNAL OF CELL SCIENCE

Patient monitoring and follow-up in lentiviral clinical trials

(2013) Gerard J. McGarrity et al.   JOURNAL OF GENE MEDICINE

Chimeric antigen receptor containing ICOS signaling domain mediates specific and efficient antitumor effect of T cells against EGFRvIII expressing glioma

(2013) Chan-Juan Shen et al.   Journal of Hematology & Oncology

Use of hollow fiber tangential flow filtration for the recovery and concentration of HIV virus-like particles produced in insect cells

(2013) Alejandro Negrete et al.   JOURNAL OF VIROLOGICAL METHODS

Treatment of Metastatic Renal Cell Carcinoma With CAIX CAR-engineered T cells: Clinical Evaluation and Management of On-target Toxicity

(2013) Cor HJ Lamers et al.   MOLECULAR THERAPY

Manufacture of Clinical-Grade CD19-Specific T Cells Stably Expressing Chimeric Antigen Receptor Using Sleeping Beauty System and Artificial Antigen Presenting Cells

(2013) Harjeet Singh et al.   PLoS One

Wrapping up the bad news – HIV assembly and release

(2013) Bo Meng et al.   Retrovirology

Lentiviral Hematopoietic Stem Cell Gene Therapy in Patients with Wiskott-Aldrich Syndrome

(2013) A. Aiuti et al.   SCIENCE

PD-1- and CTLA-4-Based Inhibitory Chimeric Antigen Receptors (iCARs) Divert Off-Target Immunotherapy Responses

(2013) V. D. Fedorov et al.   Science Translational Medicine

Characterization and comparative performance of lentiviral vector preparations concentrated by either one-step ultrafiltration or ultracentrifugation

(2013) Eleni Papanikolaou et al.   VIRUS RESEARCH

Mesothelin-Specific Chimeric Antigen Receptor mRNA-Engineered T Cells Induce Antitumor Activity in Solid Malignancies

(2013) G. L. Beatty et al.   Cancer Immunology Research

TanCAR: A Novel Bispecific Chimeric Antigen Receptor for Cancer Immunotherapy

(2013) Zakaria Grada et al.   Molecular Therapy-Nucleic Acids

Role of PI3K/Akt signaling in memory CD8 T cell differentiation

(2013) Eui Ho Kim et al.   Frontiers in Immunology

Lentiviral vectors: basic to translational

(2012) Toshie Sakuma et al.   BIOCHEMICAL JOURNAL

Targeted immunotherapy of cancer with CAR T cells: achievements and challenges

(2012) Grazyna Lipowska-Bhalla et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

CAR T cells transform to trucks: chimeric antigen receptor–redirected T cells engineered to deliver inducible IL-12 modulate the tumour stroma to combat cancer

(2012) Markus Chmielewski et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Chimeric antigen receptors for T cell immunotherapy: current understanding and future directions

(2012) Kevin J. Curran et al.   JOURNAL OF GENE MEDICINE

Combinatorial antigen recognition with balanced signaling promotes selective tumor eradication by engineered T cells

(2012) Christopher C Kloss et al.   NATURE BIOTECHNOLOGY

Downstream Processing of Lentiviral Vectors: Releasing Bottlenecks

(2012) Vanessa Bandeira et al.   Human Gene Therapy Methods

A Simple and Effective Method to Generate Lentiviral Vectors for Ex Vivo Gene Delivery to Mature Human Peripheral Blood Lymphocytes

(2012) Shicheng Yang et al.   Human Gene Therapy Methods

B-cell depletion and remissions of malignancy along with cytokine-associated toxicity in a clinical trial of anti-CD19 chimeric-antigen-receptor-transduced T cells

(2011) J. N. Kochenderfer et al.   BLOOD

A transgene-encoded cell surface polypeptide for selection, in vivo tracking, and ablation of engineered cells

(2011) X. Wang et al.   BLOOD

Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration

(2011) Katrin Zimmermann et al.   BMC BIOTECHNOLOGY

BET Bromodomain Inhibition as a Therapeutic Strategy to Target c-Myc

(2011) Jake E. Delmore et al.   CELL

Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors

(2011) H P Lesch et al.   GENE THERAPY

Morphology and separation efficiency of a new generation of analytical silica monoliths

(2011) Kristof Hormann et al.   JOURNAL OF CHROMATOGRAPHY A

Highly efficient large-scale lentiviral vector concentration by tandem tangential flow filtration

(2011) Aaron R. Cooper et al.   JOURNAL OF VIROLOGICAL METHODS

The Tumor Lysis Syndrome

(2011) Scott C. Howard et al.   NEW ENGLAND JOURNAL OF MEDICINE

T Cells with Chimeric Antigen Receptors Have Potent Antitumor Effects and Can Establish Memory in Patients with Advanced Leukemia

(2011) M. Kalos et al.   Science Translational Medicine

Lentiviral Vectors in Gene Therapy: Their Current Status and Future Potential

(2010) David Escors et al.   ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS

Genetic Modification of T Cells

(2010) Chiara Bonini et al.   BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION

Large-Scale Production Means for the Manufacturing of Lentiviral Vectors

(2010) M. Schweizer et al.   CURRENT GENE THERAPY

Large-Scale Manufacture and Characterization of a Lentiviral Vector Produced for Clinical Ex Vivo Gene Therapy Application

(2010) Otto-Wilhelm Merten et al.   HUMAN GENE THERAPY

Enhanced Tumor Trafficking of GD2 Chimeric Antigen Receptor T Cells by Expression of the Chemokine Receptor CCR2b

(2010) John A. Craddock et al.   JOURNAL OF IMMUNOTHERAPY

Case Report of a Serious Adverse Event Following the Administration of T Cells Transduced With a Chimeric Antigen Receptor Recognizing ERBB2

(2010) Richard A Morgan et al.   MOLECULAR THERAPY

T lymphocytes coexpressing CCR4 and a chimeric antigen receptor targeting CD30 have improved homing and antitumor activity in a Hodgkin tumor model

(2009) A. Di Stasi et al.   BLOOD

Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

(2009) Sven Ansorge et al.   JOURNAL OF GENE MEDICINE

Manufacturing Validation of Biologically Functional T Cells Targeted to CD19 Antigen for Autologous Adoptive Cell Therapy

(2009) Daniel Hollyman et al.   JOURNAL OF IMMUNOTHERAPY

Chimeric Receptors Containing CD137 Signal Transduction Domains Mediate Enhanced Survival of T Cells and Increased Antileukemic Efficacy In Vivo

(2009) Michael C. Milone et al.   MOLECULAR THERAPY

Chimeric Antigen Receptors Combining 4-1BB and CD28 Signaling Domains Augment PI3kinase/AKT/Bcl-XL Activation and CD8+ T Cell–mediated Tumor Eradication

(2009) Xiao-Song Zhong et al.   MOLECULAR THERAPY

Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors

(2009) Robert H Kutner et al.   Nature Protocols

Control of large, established tumor xenografts with genetically retargeted human T cells containing CD28 and CD137 domains

(2009) C. Carpenito et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Manufacturing and characterizing AAV-based vectors for use in clinical studies

(2008) J F Wright   GENE THERAPY

Good manufacturing practice and clinical-grade human embryonic stem cell lines

(2008) C. Unger et al.   HUMAN MOLECULAR GENETICS

Inducible Packaging Cells for Large-scale Production of Lentiviral Vectors in Serum-free Suspension Culture

(2008) Sophie Broussau et al.   MOLECULAR THERAPY