期刊
BIOMATERIALS
卷 63, 期 -, 页码 93-101出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.06.007
关键词
Fluorescent probe; Image analysis; Hydrogen polysulfides; Superoxide anion; Near-infrared
资金
- National Nature Science Foundation of China (NSFC) [21405172, 21275158]
- CAS [KZCX2-EW-206]
- Chinese Academy of Sciences [XDA11020702]
- program of Youth Innovation Promotion Association, CAS [2015170]
Intracellular reactive sulfur species play important roles in physiological and pathological processes. Emerging evidences suggest that sulfane sulfur instead of H2S is the actual signaling molecule in these processes. Sulfane sulfur can be generated as a result of the reaction between O-2 and H2S in mitochondria. Therefore, we develop a near-infrared mitochondria-targeting probe that allows multiresponse to O-2 and H2SO successively for investigating this biosynthetic reaction. The probe exhibits highly selective fluorescent response to O-2 and H2SO successively in presence of potential biological interferants. Fluorescent imaging studies and flow cytometry analysis of RAW264.7 cells elaborate that the probe can be used to reveal the continuous process of O-2 burst and H2SO production in situ and in real-time. The mitochondria isolation indicates that the probe can specifically localize in mitochondria. Finally, the fluorescent probe has been successfully applied to detect O-2 and H2Sn in mice. (C) 2015 Elsevier Ltd. All rights reserved.
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