Article
Environmental Sciences
Li Liu, Fang-yan Wu, Cheng-yue Zhu, Hong-yuan Zou, Rui-qi Kong, Yu-kui Ma, Dan Su, Guo-qiang Song, Yun Zhang, Ke-chun Liu
Summary: The study evaluated the neurodevelopmental toxicity of isoniazid (INH) on zebrafish embryos, finding that it inhibited hatching, caused malformations, and affected behavior and vascular development. INH exposure also led to apoptosis, decreased dopaminergic gene expression, and reduced autonomous movement in zebrafish.
Article
Pharmacology & Pharmacy
Lin Guo, Hui Gong, Ting-Li Tang, Bi-Kui Zhang, Lei-Yi Zhang, Miao Yan
Summary: The study investigated the mechanisms of crizotinib and sunitinib-induced hepatotoxicity and potential mitigation strategies through ROS and Nrf2 signaling. Both drugs activated oxidative stress and triggered hepatocyte mitochondrial apoptosis and necrosis in liver cells. ROS scavenger NAC and Nrf2 agonist tBHQ were found to alleviate cell damage and mitochondrial apoptosis during crizotinib- and sunitinib-induced hepatotoxicity.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Marine & Freshwater Biology
Bangeppagari Manjunatha, B. Deekshitha, Eunseok Seo, Jeongju Kim, Sang Joon Lee
Summary: Research shows that exposure to PM2.5 leads to increased mortality, reduced hatching rates, lower heart rates, and various developmental abnormalities in zebrafish. It also causes damage in skin cell layers, abnormalities in neurons, heart, liver, gills, brain, and eyes, and increased organelle dissolution at cellular/subcellular levels. Further investigation is needed to understand the specific elements in diesel pollution that contribute to this toxicity.
AQUATIC TOXICOLOGY
(2021)
Article
Integrative & Complementary Medicine
Duan Xiu-Ying, Ma Rui-Jiao, Hsiao Chung-Der, Jiang Zhen-Zhou, Zhang Lu-Yong, Zhang Yun, Liu Ke-Chun
Summary: This study investigated the hepatotoxicity induced by Tripterygium wilfordii multiglycoside (GTW) in zebrafish larvae, revealing that GTW can increase inflammation, enhance apoptosis, reduce cell proliferation, and cause abnormal expression of liver function-related genes, ultimately leading to liver damage.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Food Science & Technology
Jing Wu, Jiayan Li, You Wu, Mengran Yang, Yunqin Chen, Naidong Wang, Ji Wang, Zhihang Yuan, Jine Yi, Chenglin Yang
Summary: This study first revealed the protective effect of betulinic acid (BA) on the hepatotoxicity of Zearalenone (ZEA), providing a new perspective for the development of ZEA antidote and the application of BA.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Hui Fan, Jiajia Cui, Feixue Liu, Wei Zhang, Haitao Yang, Nana He, Zibo Dong, Jingquan Dong
Summary: The study suggests that malvidin is a potential protective agent against LPS-induced acute liver injury (ALI) by up-regulating the Nrf2 signaling pathway, suppressing NLRP3 inflammasome, and inhibiting apoptosis and autophagy.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Peramaiyan Rajendran, Rebai Ben Ammar, Fatma J. Al-Saeedi, Maged E. Mohamed, Medhat A. ElNaggar, Saeed Y. Al-Ramadan, Gamal M. Bekhet, Ahmed M. Soliman
Summary: The study showed that kaempferol has hepatoprotective effects against zearalenone-induced oxidative stress, likely through the PI3K/Akt-regulated Nrf2 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Dan Li, Zhi Li, Tianchang Zhang, Bo Peng, Yan Zhang, Hongwen Sun, Shuo Wang
Summary: Exposure to IQ in zebrafish resulted in liver cell damage and apoptosis, induced endoplasmic reticulum stress, and inhibited autophagy.
Article
Medicine, Research & Experimental
Zhengshan Gao, Honghong Zhan, Wei Zong, Miaomiao Sun, Lang Linghu, Guowei Wang, Fancheng Meng, Min Chen
Summary: Salidroside, extracted from Rhodiola rosea L., can protect against APAP-induced liver injury by regulating oxidative stress and inflammation.
Review
Environmental Sciences
June Zhang, Kexin Wang, Jing Guo, Yong Huang, You Wei, Kun Jia, Yuan Peng, Huiqiang Lu
Summary: This study investigated the mechanism of hepatotoxicity caused by acenaphthene using zebrafish as an experimental model. The results revealed that acenaphthene induced oxidative stress through the production of reactive oxygen species (ROS), leading to mitochondrial damage. Additionally, acenaphthene activated pathways associated with inflammation and apoptosis, resulting in hepatotoxicity and alterations in liver metabolism.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Oncology
Di Zeng, Yaping Wang, Yi Chen, Danyang Li, Guoli Li, Hanxianzhi Xiao, Jiyin Hou, Ziling Wang, Ling Hu, Lu Wang, Jing Li
Summary: ASP attenuated the 5-FU-induced Nrf2 pathway barrier to reduce oxidative stress injury and inhibit the disorder of lipid anabolism and apoptosis, providing a new protectant and novel target for treating liver injury caused by 5-FU.
FRONTIERS IN ONCOLOGY
(2021)
Article
Environmental Sciences
Nayoung Min, Hahyun Park, Taeyeon Hong, Jisoo Song, Gwonhwa Song, Whasun Lim
Summary: This study investigated the embryonic developmental toxicity of the herbicide terbutryn in zebrafish. The results showed that terbutryn exposure led to increased mortality, reduced body and eye size, and yolk sac edema in zebrafish. Fluorescence microscopy revealed the effects of terbutryn on blood vessel, motor neuron, and liver development, and apoptotic cell death was evaluated using acridine orange staining.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Hamit Emre Kizil, Cuneyt Caglayan, Ekrem Darendelioglu, Adnan Ayna, Cihan Gur, Fatih Mehmet Kandemir, Sefa Kucukler
Summary: This study reveals that morin can protect liver tissue from oxidative damage and apoptosis caused by methotrexate. Morin treatment reduces oxidative stress, apoptosis, and regulates liver function indicators and gene expressions.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Developmental Biology
Binbin Jin, Liqin Xie, Dan Zhan, Luping Zhou, Zhi Feng, Jiangyong He, Jie Qin, Congjian Zhao, Lingfei Luo, Li Li
Summary: tRNA synthetase deficiency leads to unfolded protein responses in neuronal disorders. This study identified a mutant zebrafish allele that showed increased neuronal apoptosis and compromised neurogenesis. The aberration in gene expression resulted in protein overloading and activated Perk.
Article
Environmental Sciences
Zhonghui Bai, Kun Jia, Guilan Chen, Xinjun Liao, Zigang Cao, Yangqi Zhao, Chunping Zhang, Huiqiang Lu
Summary: Exposure to CBZ induced severe hepatotoxicity in zebrafish by promoting lipid accumulation, generating excessive ROS production, and inhibiting the Wnt/beta-catenin signaling pathway. These findings highlight the environmental concerns associated with CBZ exposure.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Andrew Yau Wah Young, Gilbert Audira, Ferry Saputra, Honeymae C. Alos, Charlaine A. Aventurado, Yu-Heng Lai, Ross D. Vasquez, Chung-Der Hsiao, Chih-Hsin Hung
Summary: This study investigated the potential cardio and neural toxicity of p-Toluene sulfonamide (p-TSA) in sublethal concentrations using zebrafish as an in vivo animal model. The results showed that the overall toxicity of p-TSA was relatively low in zebrafish larvae, with minor alterations observed in the cardiac rhythm and physiology. However, acute exposure to p-TSA significantly increased locomotion activity, while prolonged exposure reduced the locomotor startle reflex activities in zebrafish. The study also revealed higher respiratory rate and blood flow velocity in acutely treated fish groups. Molecular docking analysis provided insights into the potential molecular mechanism of p-TSA on observed altered responses and minor altered vascular performance in zebrafish larvae.
Article
Infectious Diseases
Michael Edbert Suryanto, Chun-Chuen Yang, Gilbert Audira, Ross D. Vasquez, Marri Jmelou M. Roldan, Tzong-Rong Ger, Chung-Der Hsiao
Summary: This study analyzed the potential adverse effects of antibiotics on zebrafish behavior using a phenomic approach. The results showed that certain antibiotics can cause behavioral alterations in adult zebrafish, even at low concentrations. These alterations are closely related to neurological motor impairments, anxiety levels, and stress responses.
Article
Biology
Gilbert Audira, Michael Edbert Suryanto, Kelvin H-C Chen, Ross D. Vasquez, Marri Jmelou M. Roldan, Chun-Chuen Yang, Chung-Der Hsiao, Jong-Chin Huang
Summary: This study found that amputation in the caudal fin resulted in the most pronounced behavior alterations in zebrafish and that these changes were caused by pain elicited from the fin amputation. Treatment with lidocaine could alleviate the behavioral effects but did not accelerate the recovery process.
Article
Environmental Sciences
Ying-Ting Lin, Rong-Xuan Liu, Gilbert Audira, Michael Edbert Suryanto, Marri Jmelou M. Roldan, Jiann-Shing Lee, Tzong-Rong Ger, Chung-Der Hsiao
Summary: This study comprehensively explores the key factors of lanthanide complex electronic structures for the acute toxicity of rare earth elements (REEs) using zebrafish as a model animal. The research reveals a significant correlation between the toxicity of REEs and their atomic numbers, Milliken charge, and aromatic C-avg charge. Additionally, the disruption of photo motor response activity in zebrafish embryos is observed to evaluate lanthanide toxicity.
Article
Medicine, Research & Experimental
Petrus Siregar, Gilbert Audira, Agnes L. Castillo, Marri Jmelou M. Roldan, Michael Edbert Suryanto, Rong-Xuan Liu, Ying-Ting Lin, Yu-Heng Lai, Chung-Der Hsiao
Summary: This study compares the effects of four primary alkaloids isolated from areca palm nut on zebrafish behaviors. The results show that these alkaloids induce hyperactivity-like behaviors, alter social behaviors, and cause changes in fear response in zebrafish. Molecular docking experiments suggest that these effects may be a result of the interaction between alkaloids and multiple receptors in the nervous system.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Huan-Chau Lin, Ferry Saputra, Gilbert Audira, Yu-Heng Lai, Marri Jmelou M. Roldan, Honeymae C. Alos, Charlaine A. Aventurado, Ross D. Vasquez, Guan-Jhe Tsai, Ken-Hong Lim, Chung-Der Hsiao
Summary: This study aimed to observe the potential cardiovascular-related side effects after co-exposure to ASC and PON using zebrafish as an animal model. Zebrafish acutely exposed to both compounds showed no significant change in cardiac performance parameters at the no observed effect concentration (NOEC). However, a significant increase in nkx2.5 expression level and a substantial decrease in blood flow velocity were recorded, suggesting that combining these compounds at NOEC can cause mild cardiovascular-related side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Kumail Abbas, Aqeel Raza, Ross D. Vasquez, Marri Jmelou M. Roldan, Nemi Malhotra, Jong-Chin Huang, Olivia E. M. Buenafe, Kelvin H-C Chen, Shih-Shin Liang, Chung-Der Hsiao
Summary: This article provides information on the application, detection methods, conflicts, and legal divisions of Ractopamine (RAC), emphasizing its potential toxicities on aquatic animals and the potentially harmful effects on the aquatic environment and human health.
Article
Biology
Ali Farhan, Ferry Saputra, Michael Edbert Suryanto, Fahad Humayun, Roi Martin B. Pajimna, Ross D. Vasquez, Marri Jmelou M. Roldan, Gilbert Audira, Hong-Thih Lai, Yu-Heng Lai, Chung-Der Hsiao
Summary: This study introduces a software package called OpenBloodFlow, which accurately measures blood flow velocity and blood cell count using the dorsal aorta of zebrafish. The program is based on the Python programming language and offers a user-friendly graphical user interface and automatic result saving. Compared to commercial software, OpenBloodFlow is cost-effective and easy to use.
Article
Biology
Michael Edbert Suryanto, Ferry Saputra, Kevin Adi Kurnia, Ross D. Vasquez, Marri Jmelou M. Roldan, Kelvin H-C Chen, Jong-Chin Huang, Chung-Der Hsiao
Summary: In this study, we explored the feasibility of using a deep learning tool called DeepLabCut (DLC) for markerless cardiac physiology assessment in zebrafish embryos. The trained DLC model was able to accurately detect multiple cardiac parameters and outperformed other traditional methods. This new technique provides automation, precise detection, and real-time labeling, and can be applied to analyze zebrafish with cardiovascular defects. The results of this study contribute to a better understanding of the cardiovascular system and abnormalities.
Article
Engineering, Multidisciplinary
Gilbert Audira, Ting-Wei Hsu, Kelvin H. -C. Chen, Jong-Chin Huang, Ming-Der Lin, Tzong-Rong Ger, Chung-Der Hsiao
Summary: Image stacking is a crucial method for micro or macro photography. This study presents a cost-effective method that can achieve image focus extensions comparable to traditional methods. It offers shorter operation time and higher flexibility, making it a promising alternative for scientists.
Article
Nanoscience & Nanotechnology
Jolleen Natalie I. Balitaan, Wen-Jie Luo, Yi-Wen Su, Ching-Yun Yu, Tai-Yi Wu, Chen-An Chang, Hsi-Wei Jia, Shu-Rung Lin, Chung-Der Hsiao, Jui-Ming Yeh
Summary: The high prevalence of skin wounds and antibiotic-resistant strains pose a threat to society. Hydrogels, specifically the glycol chitosan/dibenzaldehyde-terminated polyethylene glycol hydrogels (GC/DP), were developed and found to have self-healing abilities and inhibitory effects against E. coli, P. aeruginosa, and S. aureus. In zebrafish embryo models, GC/DP hydrogels showed high biocompatibility and a significant wound contraction rate. This study is the first to develop injectable hydrogels for wound healing with innate bacteriostatic properties against these three bacteria.
ACS APPLIED BIO MATERIALS
(2023)
Article
Marine & Freshwater Biology
Ferry Saputra, Michael Edbert Suryanto, Gilbert Audira, Cao Thang Luong, Chih-Hsin Hung, Marri Jmelou Roldan, Ross D. Vasquez, Chung-Der Hsiao
Summary: This study presents a simple and automatic method to analyze the cardiac performance of Daphnia using DeepLabCut software. The use of macro scripts in data summarization and comparison with manual methods further enhances the efficiency of analysis. The study also demonstrates the potential cardiotoxic effects of imidacloprid and pendimethalin on D. magna, highlighting the importance of this method for fast and accurate cardiotoxicity measurements.
AQUATIC TOXICOLOGY
(2023)
Article
Environmental Sciences
Kevin Adi Kurnia, Ying-Ting Lin, Ali Farhan, Nemi Malhotra, Cao Thang Luong, Chih-Hsin Hung, Marri Jmelou M. Roldan, Che-Chia Tsao, Tai-Sheng Cheng, Chung-Der Hsiao
Summary: In recent years, efforts have been made to utilize surface water for various purposes, but the presence of contaminants introduced by human activities has hindered these efforts. Herbicides such as Glyphosate and Glufosinate are known to contaminate surface water through agriculture, while emerging contaminants like rare earth elements enter through electronic waste. Duckweeds, specifically from the Wolffia genus, are used as indicators of metal pollutants in aquatic environments. In this study, a machine learning-based tool called StarDist integrated with ImageJ and Python was used for automatic frond counting and toxicity analysis, with Dysprosium found to be the most toxic and Samarium the least toxic.
Article
Environmental Sciences
Michael Edbert Suryanto, Gilbert Audira, Marri Jmelou M. Roldan, Hong-Thih Lai, Chung-Der Hsiao
Summary: The study replicated and optimized a method to evaluate color preference in freshwater crayfish based on species, life stages, sex, and pharmaceutical exposure. Two crayfish species showed color preferences, with P. clarkii displaying a stronger preference for red and C. quadricarinatus displaying a dislike for yellow. The study also found that waterborne exposure to the antidepressant sertraline can alter the color preference of P. clarkii.
Article
Medicine, Research & Experimental
Gilbert Audira, Jong-Chin Huang, Kelvin H. -C. Chen, Kevin Adi Kurnia, Ross D. Vasquez, Marri Jmelou M. Roldan, Yu-Heng Lai, Chung-Der Hsiao, Cheng-Yo Yen
Summary: The usage of zebrafish for pain studies has increased recently, and this study evaluated the effects of different analgesics on the behavior of fin-amputated zebrafish. Five painkillers were found to potentially improve the behavior of amputated fish. The combination of indomethacin and diclofenac showed prominent effects in recovering zebrafish behaviors.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
