期刊
BIOMATERIALS
卷 57, 期 -, 页码 33-40出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.03.059
关键词
Titanium dioxide nanoparticle; Inerleukin-1 beta; Inflammation; Human gingival fibroblast; Metabolomics
资金
- Ministry of Education, Culture, Sports, Science and Technology [25670897]
- Meikai University School of Dentistry
- Yamagata Prefectural Government
- Tsuruoka City, Japan
- Grants-in-Aid for Scientific Research [25670897] Funding Source: KAKEN
Although nanoparticles (NPs) has afforded considerable benefits in various fields of sciences, several reports have shown their harmful effects, suggesting the necessity of adequate risk assessment. To clarify the mechanism of titanium dioxide nanoparticles (TiO2 NPs)-enhanced gingival inflammation, we conducted the full-scale metabolomic analyses of human gingival fibroblast cells treated with IL-1 beta alone or in combination with TiO2 NPs. Observation with transmission electron microscope demonstrated the incorporation of TiO2 NPs into vacuoles of the cells. TiO2 NPs significantly enhanced the IL-1 beta-induced prostaglandin E-2 production and COX-1 and COX-2 protein expression. IL-1 beta reduced the intracellular concentrations of overall primary metabolites especially those of amino acid, urea cycle, polyamine, S-adenosylmethione and glutathione synthetic pathways. The addition of TiO2 NPs further augmented these IL-1 beta-induced metabolic changes, recommending careful use of dental materials containing TiO2 NPs towards patients with gingivitis or periodontitis. The impact of the present study is to identify the molecular targets of TiO2 NPs for the future establishment of new metabolic markers and therapeutic strategy of gingival inflammation. (C) 2015 Elsevier Ltd. All rights reserved.
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