Article
Polymer Science
Jingjing Mai, Steven Pratt, Bronwyn Laycock, Clement Matthew Chan
Summary: This study explores the synthesis of industrially relevant high-molecular-weight block copolymers PHBV-b-PHBV using high molecular weight hydroxy-functionalised PHBV starting materials coupled with facile diisocyanate chemistry. Two-step synthesis approach and one-step approach were compared, and both successfully produced block copolymers. However, the two-step synthesis was less effective in building molecular weight. Both synthetic approaches were affected by additional isocyanate reactions resulting in the formation of by-products, affecting the product performance.
Article
Polymer Science
Maryam Abbasi, Dikshya Pokhrel, Erik R. Coats, Nicholas M. Guho, Armando G. McDonald
Summary: The thermal, mechanical, and rheological behavior of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) synthesized by mixed microbial consortia (MMC) fed fermented dairy manure at large-scale level was studied. The chemical structure of the extracted PHBV biopolymers was analyzed, and it was found that the PHBV had a random distribution of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units with 3HV content between 16% and 24%. The glass transition temperature (T-g) of the extracted PHBV varied between -0.7 and -7.4 degrees C.
Article
Polymer Science
Jingjing Mai, Clement Matthew Chan, Bronwyn Laycock, Steven Pratt
Summary: This study investigates the reaction kinetics of high molecular weight poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers containing primary and secondary hydroxyl groups as well as terminal carboxylic acid groups. The effects of molecular weight, [NCO]/[OHtotal], and reaction time were studied to facilitate the use of these copolymers in block copolymer synthesis. The results show that primary hydroxyl groups reacted faster than secondary hydroxyl groups, while the carboxylic acid groups did not react with isocyanate. This work provides a basis for controlled PHBV-based block copolymer synthesis.
Article
Biochemistry & Molecular Biology
Viviana Urtuvia, Belen Ponce, Rodrigo Andler, Alvaro Diaz-Barrera
Summary: This study evaluated PHBV films with different 3-hydroxyvalerate (3HV) contents produced by Azotobacter vinelandii OP. The results showed that a higher 3HV content improved the thermomechanical properties of PHBV films, reduced the polymer melting point, and affected the tensile strength. Additionally, thermomechanical properties played an important role in biodegradation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Polymer Science
Beatriz Melendez-Rodriguez, Sergio Torres-Giner, Maria A. M. Reis, Fernando Silva, Mariana Matos, Luis Cabedo, Jose Maria Lagaron
Summary: A new P(3HB-co-3HV-co-3HHx) terpolyester synthesized using fruit pulp as feedstock was melt-mixed with commercial PHBV to create miscible films for food packaging. As the content of P(3HB-co-3HV-co-3HHx) increased, the mechanical response of the films transitioned from brittle to ductile, with increased permeabilities to gases.
Article
Biochemistry & Molecular Biology
Young Yun Jo, Soyoung Park, Gyeongtaek Gong, Soonjong Roh, Jin Yoo, Jung Ho Ahn, Sun-Mi Lee, Youngsoon Um, Kyoung Heon Kim, Ja Kyong Ko
Summary: This paper reports an improved artificial pathway for enhancing the 3HV component during PHBV biosynthesis from a structurally unrelated carbon source by Cupriavidus necator H16. By genetically manipulating the branched-chain amino acid pathways, a recombinant strain was developed to increase the intracellular accumulation of propionyl-CoA, a key precursor of the 3HV monomer. This recombinant strain produced 42.5% (g PHBV/g dry cell weight) PHBV with 64.9 mol% 3HV monomer from fructose as the sole carbon source.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Environmental
Jingjing Mai, Christopher J. Garvey, Clement Matthew Chan, Steven Pratt, Bronwyn Laycock
Summary: Polyhydroxyalkanoates (PHAs) are attractive alternatives to petroleum-derived plastics, as they are derived from bacteria, thermoprocessible, and truly biodegradable. However, the common short chain length PHAs, such as poly(3-hydroxybutyrate) (P3HB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), are relatively stiff and brittle. This study demonstrates the synthesis and characterization of novel high molecular weight PHBV-b-PHBV-b-PHBV block copolymers, which show significantly improved mechanical properties compared to the starting materials. This approach offers an attractive platform for the production of biodegradable PHA materials for various applications.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Engineering, Environmental
Mohammad I. Ibrahim, Diya Alsafadi, Khalid A. Alamry, Mahmoud A. Hussein
Summary: PHBV, a biopolyester with biodegradability, biocompatibility, and being biobased, shows great potential to replace conventional non-degradable polymers. Its weaknesses in mechanical properties and processability drive research towards developing composite materials for various applications.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2021)
Article
Polymer Science
Hau Seung Jeremy Wong, Kesaven Bhubalan, Al-Ashraf Abdullah Amirul
Summary: Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) is a widely studied short-chain-length polyhydroxyalkanoates (PHA) with important applications in various fields. However, the high cost and preference of propionate and valerate over other 3-hydroxyvalerate (3HV) precursors have hindered the development of diverse production processes. Alkyl alcohols, produced from inexpensive starting materials through oxo synthesis, offer a cost-effective advantage and can be biosynthesized from natural substrates and organic wastes. Despite their potential, the toxicity of alkyl alcohols to most PHA-producing bacteria has limited their widespread use as 3HV precursors. However, the continuous discovery of alkyl alcohols-utilizing PHA-producing bacteria has provided more options for 3HV precursor selection, leading to higher economic feasibility in P(3HB-co-3HV) production processes.
Article
Biochemistry & Molecular Biology
Changfeng Miao, Dong Meng, Yuling Liu, Fang Wang, Lu Chen, Zhaosong Huang, Xiangyu Fan, Pengfei Gu, Qiang Li
Summary: This study identified a phaC(R) gene encoding PHA synthase in Rhodoligotrophos defluvii, and successfully synthesized different content and structural PHA copolymers under different carbon source conditions, achieving efficient biosynthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Sara Alfano, Francesca Pagnanelli, Andrea Martinelli
Summary: Polyhydroxyalkanoates (PHAs) are degradable polyesters with the potential to replace oil-based polymers. This study presents a new method using attenuated total reflection FTIR (ATR-FTIR) to rapidly determine the composition of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV), the most common PHA copolymer. The method proves to be useful for easy and rapid estimation of P3HBV composition.
Article
Biochemistry & Molecular Biology
Beatriz Melendez-Rodriguez, Maria A. M. Reis, Monica Carvalheira, Chris Sammon, Luis Cabedo, Sergio Torres-Giner, Jose Maria Lagaron
Summary: This study developed three new copolymers of PHBV with different 3HV contents using cheese whey as feedstock. The resultant PHBV biopapers showed high transparency but slightly yellow color. The 40% 3HV sample exhibited the highest ductility and toughness among the three copolymers.
Article
Polymer Science
Aline Luiza M. Carlos, Javier Mauricio A. Mancipe, Marcos L. Dias, Rossana M. S. M. Thire
Summary: Electrospinning is commonly used for micro- and nanofibers production, while solution blow spinning (SBS) provides a safer alternative for certain applications. In this study, core-shell fibers were produced using SBS with PHBV showing better morphology and stability. The core-shell structure allows for sustained release of bioactive agents, and rotating collectors can produce smaller diameter fibers effectively.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Isabela F. Santos, Rodolfo M. Moraes, Simone F. Medeiros, Jaspreet K. Kular, Marcus A. Johns, Ram Sharma, Amilton M. Santos
Summary: Polymeric nanoparticles were developed without any matrix ligands on their surface to promote cell attachment and proliferation of human osteoblasts. The study demonstrated that cells readily attached and spread onto the nanoparticle surfaces compared to non-tissue culture polystyrene, indicating the potential of PHBV nanoparticles for cell attachment and growth in tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Multidisciplinary
Silvia Bittolo Bon, Irene Chiesa, Davide Morselli, Micaela Degli Esposti, Paola Fabbri, Carmelo De Maria, Tommaso Foggi Viligiardi, Antonino Morabito, Giacomo Giorgi, Luca Valentini
Summary: This study reports the fabrication of two different three-dimensional architectures of regenerated silk (RS) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with embedded functionalities, which have potential clinical applications. Experimental data are supported by finite element simulations, demonstrating the possibility to program the shape-changing behavior of 3D printed structures. The synergic effect obtained by combining materials on appropriate architectures paves the way to potential clinical applications.
MATERIALS & DESIGN
(2021)
Correction
Biotechnology & Applied Microbiology
Xiao-Bin Liu, Lin-Ping Wu, Jing Hou, Jun-Yu Chen, Jing Han, Hua Xiang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Lin-Ping Wu, Davoud Ahmadvand, Junan Su, Arnaldur Hall, Xiaolong Tan, Z. Shadi Farhangrazi, S. Moein Moghimi
NATURE COMMUNICATIONS
(2019)
Review
Materials Science, Biomaterials
Lin-Ping Wu, Danyang Wang, Zibiao Li
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Martina Pannuzzo, Sara Esposito, Lin-Ping Wu, Jaehong Key, Santosh Aryal, Christian Celia, Luisa di Marzio, Seyed Moein Moghimi, Paolo Decuzzi
Article
Nanoscience & Nanotechnology
Lan Wu, Lin-Ping Wu, Jingya Wu, Jin Sun, Zhonggui He, Cristina Rodriguez-Rodriguez, Katayoun Saatchi, Lea Ann Dailey, Urs O. Hafeli, Dongmei Cun, Mingshi Yang
Summary: This study found that LPNs showed a prolonged and enhanced gene silencing effect compared to LPXs after pulmonary administration, possibly due to the sustained release characteristics of PLGA and the more stable retention of LPNs in lung tissue. Additionally, the presence of PLGA effectively alleviated the acute inflammatory effect of cationic lipids on the lungs. The findings suggest that PLGA-based LPNs may be an effective formulation strategy for sustained gene silencing effects in the lung via pulmonary administration.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Aristides D. Tagalakis, Vignesh Jayarajan, Ruhina Maeshima, Kin H. Ho, Farhatullah Syed, Lin-Ping Wu, Ahmad M. Aldossary, Mustafa M. Munye, Talisa Mistry, Olumide Kayode Ogunbiyi, Arturo Sala, Joseph F. Standing, Seyed M. Moghimi, Andrew W. Stoker, Stephen L. Hart
Summary: This study introduces a novel formulation of siRNA nanoparticles, termed RTNs, which can target tumors effectively while minimizing clearance by the liver, resulting in predominant accumulation in tumors rather than other organs in the body. The experiment demonstrates that these siRNA nanoparticles significantly retard the growth of xenograft tumors and improve survival rates, showing promise for delivering targeted siRNA therapeutics for cancer treatment.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Multidisciplinary Sciences
Lin-Ping Wu, Mario Ficker, Jorn B. Christensen, Dmitri Simberg, Panagiotis N. Trohopoulos, Seyed M. Moghimi
Summary: The study finds that sub 6 nm dendrimers can evade complement activation but interact with IgM to trigger lectin-pathway complement activation. Understanding the interaction of nanomaterials with complement is crucial for developing complement-safe nanomedicines and medical devices.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Weiyue Deng, Yuying Zhang, Wei Wang, Aishi Song, Omar Mukama, Jiarong Huang, Xiaobo Han, Sihao Deng, Zuoxian Lin, Jean du Dieu Habimana, Rongqi Huang, Kexin Peng, Bing Ni, Shusheng Zhang, Xiaoxin Yan, Ji Li, Lin-Ping Wu, Zhiyuan Li
Summary: This study established a new therapeutic approach for alopecia areata using hair follicle-derived MSCs, which showed significant improvement in hair loss and reduction in inflammation both in vitro and in vivo models.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Pharmacology & Pharmacy
Baozhao Xie, Keqian Du, Fujian Huang, Zhiming Lin, Linping Wu
Summary: This article reviews the potential and strategies of cationic nanomaterials in the treatment of autoimmune diseases, and discusses the challenges and possible solutions encountered during the development of cationic materials-based therapeutics for autoimmune diseases.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Congqi Hu, Zhao Dai, Jia Xu, Lianyu Zhao, Yanping Xu, Meilin Li, Jiahui Yu, Lu Zhang, Hui Deng, Lijuan Liu, Mingying Zhang, Jiarong Huang, Linping Wu, Guangxing Chen
Summary: This study identified the serum marker ORM1 as a significant differential expression between normal and RA patient samples, positively correlated with disease activity, offering a new perspective for future research on RA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jinfei Hou, Xu Zhang, Yuqiong Wu, Junjin Jie, Zhenxing Wang, Guo-Qiang Chen, Jiaming Sun, Lin-Ping Wu
Summary: Organohydrogels with stability and good biocompatibility were successfully fabricated using click chemistry and the combination of two-dimensional materials. These hydrogels demonstrated fatigue resistance, long-term stability in body fluids, and exhibited excellent performance in in vitro and in vivo experiments.
Article
Immunology
Qinhai Ma, Runfeng Li, Jianmin Guo, Man Li, Lin Ma, Jun Dai, Yongxia Shi, Jinlong Dai, Yuankeng Huang, Cailing Dai, Weiqi Pan, Huiling Zhong, Hong Zhang, Jian Wen, Haoting Zhao, Linping Wu, Wei Yang, Biliang Zhang, Zifeng Yang
Summary: RBMRNA-176 is a broad-spectrum and protective vaccine candidate for COVID-19 that can generate high titer antibodies and neutralize different variants of SARS-CoV-2. It also demonstrates effective control of viral replication and lung injury in animal studies.
Review
Biochemistry & Molecular Biology
Chan Huang, Weiyan Yuan, Jun Chen, Lin-Ping Wu, Tianhui You
Summary: Diabetes mellitus has become one of the fastest-growing health crises due to its various complications, including diabetic foot, which can seriously affect patients' life and health. Smart biomaterials have been developed to improve the healing process and outcomes of diabetic wound treatment by acting as a drug delivery system and responsive to the wound microenvironment.
Review
Polymer Science
Jun Chen, Di Zhang, Lin-Ping Wu, Ming Zhao
Summary: Blood vessels are vital in delivering oxygen and nutrients to organs as well as regulating tissue regeneration. Bioengineered vascular grafts have emerged as a promising solution for treating damaged or occluded vessels. This review discusses the materials and techniques used in engineering tubular scaffolds and vasculature at various levels, and provides examples of vascularized tissue engineering in different organs. The current challenges and future developments in biofunctional engineered vessels are also explored.
Article
Engineering, Biomedical
Jing Han, Lin-Ping Wu, Xiao-Bin Liu, Jing Hou, Li-Li Zhao, Jun-Yu Chen, Da-He Zhao, Hua Xiang
