Article
Chemistry, Multidisciplinary
Nicolo Tonali, Loreen Hericks, David C. Schroeder, Oliver Kracker, Radoslaw Krzemieniecki, Julia Kaffy, Vadim Le Joncour, Pirjo Laakkonen, Antoine Marion, Sandrine Ongeri, Veronica I. Dodero, Norbert Sewald
Summary: Peptidotriazolamers can act as modulators of Aβ(1-42) oligomerization, with some active peptidotriazolamers able to interfere with the formation of toxic early Aβ oligomers and even cross the blood-brain barrier, demonstrating potential for drug development applications.
Article
Cell Biology
Jiewei Lin, Zhiwei Xu, Junjie Xie, Xiaxing Deng, Lingxi Jiang, Hao Chen, Chenghong Peng, Hongwei Li, Jiaqiang Zhang, Baiyong Shen
Summary: APOL1 is aberrantly expressed in pancreatic cancer and associated with poor prognosis. Functional experiments showed that APOL1 promotes proliferation and inhibits apoptosis in pancreatic cancer through activating NOTCH1 signaling pathway.
CELL DEATH & DISEASE
(2021)
Article
Biochemical Research Methods
Julia A. Townsend, Michael T. Marty
Summary: Membrane proteins often form functional complexes in lipid bilayers, making it difficult to characterize their oligomerization. However, using native mass spectrometry combined with lipid nanodiscs, it is possible to study the oligomeric state distribution and lipid preferences of these complexes. This study presents a novel data analysis method using macromolecular mass defect analysis to interpret complex spectra. It also discusses the limitations and strategies to resolve ambiguities, along with recent research on antimicrobial peptides, amyloid proteins, and viroporins in lipid membranes.
Article
Biochemistry & Molecular Biology
Thomas L. Genetta, Joshua C. Hurwitz, Evan A. Clark, Benjamin T. Herold, Shadi Khalil, Tarek Abbas, James M. Larner
Summary: The transcriptional repressor ZEB1 interacts with 53BP1 and enhances Non-Homologous End Joining (NHEJ) repair while inhibiting Homologous Recombination (HR) repair. It also promotes resection at DSBs and confers PARP inhibitor (PARPi) sensitivity on BRCA1-deficient cells. These findings suggest that ZEB1 levels may serve as a predictive biomarker for PARPi response in BRCA1-deficient tumors.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Luca M. Lauth, Bruno Voigt, Twinkle Bhatia, Lisa Machner, Jochen Balbach, Maria Ott
Summary: In acidic secretory granules of mammalian cells, peptide hormones may be stored as functional amyloid fibrils, and polyanion heparin can promote the fibril formation of PTH.
Article
Cell Biology
Ganggang Wang, Jinghua Li, Ye Yao, Yingyi Liu, Peng Xia, Hao Zhang, Maohui Yin, Zhixiang Qin, Weijie Ma, Yufeng Yuan
Summary: Recent studies have identified SNORA42 as an oncogene in hepatocellular carcinoma, with overexpression associated with poor prognosis. It may serve as a potential therapeutic target and prognostic biomarker.
CELL DEATH DISCOVERY
(2021)
Article
Fisheries
Xin Zhao, Wenji Huang, Jiahong Guo, Ning Ji, Jianhua Feng, Kangyong Chen, Jun Zou, Jun Zou
Summary: The study found that PLAAT1 is involved in the interplay between p53 mediated cellular responses and autophagy.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Cell Biology
Zhiqi Zhang, Haitao Yu, Wenyan Yao, Na Zhu, Ran Miao, Zhiquan Liu, Xuwei Song, Chunhua Xue, Cheng Cai, Ming Cheng, Ke Lin, Dachuan Qi
Summary: This study reveals that RRP9 plays a critical role in inducing gemcitabine chemoresistance in pancreatic cancer through the activation of the IGF2BP1/AKT signaling pathway.
CELL COMMUNICATION AND SIGNALING
(2022)
Review
Cell Biology
Robert G. Parton, Vikas Tillu, Kerrie-Ann McMahon, Brett M. Collins
Summary: Caveolae are plasma membrane pits formed by a coordination of various proteins and lipids, with filamentous cavin proteins, membrane-embedded caveolin proteins, and specific plasma membrane lipids playing key roles. The protein assembly involves multiple low-affinity interactions, with cavins helping to create a lipid domain conducive to caveolin insertion. This synergistic assembly supports the formation of a metastable membrane domain that can be easily disassembled in response to cellular stress or endocytic trafficking.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Cell Biology
Fabio Lolicato, Roberto Saleppico, Alessandra Griffo, Annalena Meyer, Federica Scollo, Bianca Pokrandt, Hans-Michael Muller, Helge Ewers, Hendrik Hahl, Jean-Baptiste Fleury, Ralf Seemann, Martin Hof, Britta Brugger, Karin Jacobs, Ilpo Vattulainen, Walter Nickel
Summary: This study found that cholesterol can promote the recruitment and translocation of FGF2 in cells, as well as enhance its binding to PI(4,5)P2. Through molecular dynamics simulations and experiments, it was revealed that cholesterol modulates the binding of FGF2 to PI(4,5)P2 by increasing the visibility of PI(4,5)P2 on the membrane surface, inducing the clustering of PI(4,5)P2 molecules, and increasing membrane tension.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Qiang Guo, Ximiao Chen, Jiaoxiang Chen, Gang Zheng, Chenglong Xie, Hongqiang Wu, Zhimin Miao, Yan Lin, Xiangyang Wang, Weiyang Gao, Xiangtao Zheng, Zongyou Pan, Yifei Zhou, Yaosen Wu, Xiaolei Zhang
Summary: The study investigated the role of STING in osteoarthritis progression, revealing its involvement in ECM metabolism, apoptosis, and senescence in chondrocytes. STING activation of NF-kappa B signaling cascade was found to contribute to OA development, with suppression of STING showing potential as a therapeutic approach.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Joan Patrick, Pontus Pettersson, Lena Maler
Summary: WaaG is a glycosyltransferase involved in bacterial cell wall synthesis. The study used 5-fluoro-Trp labelling and NMR techniques to investigate the dynamic interactions of WaaG with lipids and the donor substrate. The findings provide important insights into the function and structure of WaaG in a biologically relevant environment.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Chemistry, Multidisciplinary
Simli Dey, Dayana Surendran, Oskar Engberg, Ankur Gupta, Sashaina E. Fanibunda, Anirban Das, Barun Kumar Maity, Arpan Dey, Vicky Visvakarma, Mamata Kallianpur, Holger A. Scheidt, Gilbert Walker, Vidita A. Vaidya, Daniel Huster, Sudipta Maiti
Summary: Serotonin can modulate membrane properties and cellular function by directly binding to the membrane, independent of receptor activity. This interaction potentiates key cellular processes, such as transferrin receptor endocytosis, in a receptor-independent fashion.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Immunology
Yuting Wang, Wenjie Xi, Xinyi Zhang, Xinwen Bi, Boyang Liu, Xiaoming Zheng, Xinjin Chi
Summary: Acute kidney injury is a common and severe complication of sepsis, and the underlying mechanisms are poorly understood. This study established mouse models and in vitro cell models to investigate the role of lysosomal membrane permeabilization (LMP) and cathepsin B (CTSB) in sepsis-induced acute kidney injury (S-AKI). The results suggest that inhibiting CTSB activity may be a potential therapeutic strategy for alleviating S-AKI.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yingming Sun, Xiaoge Sun, Chengcheng You, Shijing Ma, Yuan Luo, Shan Peng, Fang Tang, Xiaoli Tian, Feng Wang, Zhengrong Huang, Hongnv Yu, Yu Xiao, Xiaoyong Wang, Junhong Zhang, Yan Gong, Conghua Xie
Summary: MUC3A is highly expressed in non-small cell lung cancer (NSCLC) and associated with poor prognosis, advanced staging, and low differentiation. Knockdown of MUC3A suppresses tumor growth and DNA damage repair pathways, suggesting it may be a potential oncogene.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Michael Trichet, Rosamaria Lappano, Mathilde Belnou, Lilian Shadai Salazar Vazquez, Isabel Alves, Delphine Ravault, Sandrine Sagan, Lucie Khemtemourian, Marcello Maggiolini, Yves Jacquot
Article
Multidisciplinary Sciences
Marie Glavier, Dhenesh Puvanendran, Dimitri Salvador, Marion Decossas, Gilles Phan, Cyril Garnier, Elisa Frezza, Quentin Cece, Guy Schoehn, Martin Picard, Jean-Christophe Taveau, Laetitia Daury, Isabelle Broutin, Olivier Lambert
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Narek Yousefian, Alina Ornik-Cha, Sylvie Poussard, Marion Decossas, Melanie Berbon, Laetitia Daury, Jean-Christophe Taveau, Jean-William Dupuy, Selena Dordevic-Marquardt, Olivier Lambert, Klaas M. Pos
Summary: This study investigates the structure and function of the Gram-negative bacteria efflux system, revealing the collaboration of inner membrane transporter, outer membrane channel, and periplasmic adaptor protein in the EmrAB-TolC tripartite system. Through electron microscopy, a 33 nm long complex stabilized by Amphipol belt was observed, suggesting a potential tip-to-tip interaction between EmrA and TolC.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Review
Biochemistry & Molecular Biology
Estelle Rascol, Sandrine Villette, Etienne Harte, Isabel D. Alves
Summary: Plasmon waveguide resonance (PWR) is a variant of surface plasmon resonance (SPR) invented about two decades ago at the University of Arizona. PWR offers the advantage of monitoring both mass and structural changes, in addition to characterizing the kinetics and affinity of molecular interactions. It is especially useful for studying molecular interactions in anisotropic-oriented thin films.
Article
Infectious Diseases
Esther Boyer, Jean Dessolin, Margaux Lustig, Marion Decossas, Gilles Phan, Quentin Cece, Gregory Durand, Veronique Dubois, Joris Sansen, Jean-Christophe Taveau, Isabelle Broutin, Laetitia Daury, Olivier Lambert
Summary: This study reveals the Q93R MexA mutation and the OprM C-terminal peptide as molecular determinants modulating the assembly process efficacy with cognate and non-cognate OMFs, even though they are outside the interfacial contact. It provides insights into how OMF selectivity operates during the formation of the tripartite complex.
Article
Biochemistry & Molecular Biology
Rim Baccouch, Estelle Rascol, Kaja Stoklosa, Isabel D. Alves
Summary: GPCRs are membrane proteins that sense extracellular signals and activate intracellular signaling pathways. Their structure and function are influenced by the surrounding lipid environment and membrane properties. Lipids can act as important cofactors and play a role in GPCR activity.
BIOPHYSICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Estelle Rascol, Anouk Dufourquet, Rim Baccouch, Pierre Soule, Isabel D. Alves
Summary: A novel experimental procedure using microscale thermophoresis (MST) directly from cell membrane fragments was developed to determine ligand binding affinities to membrane proteins. The procedure overcame the challenge of purification and successfully determined the concentration and binding affinity of the dopamine D2 receptor.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Rim Baccouch, Yarong Shi, Emilie Vernay, Marion Mathelie-Guinlet, Nada Taib-Maamar, Sandrine Villette, Cecile Feuillie, Estelle Rascol, Philippe Nuss, Sophie Lecomte, Michael Molinari, Galya Staneva, Isabel D. Alves
Summary: The lipid composition and balance in cellular membranes can be influenced by various factors, including aging, pathologies, and diets. In individuals with psychiatric disorders, such as schizophrenia, the cell membranes have been found to be deficient in polyunsaturated fatty acids (PUFAs), which could impact the interaction of medications and the activation of receptors embedded in the lipid membrane. Understanding how changes in PUFA levels affect membrane properties is crucial.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Biochemistry & Molecular Biology
Marie-Lise Jobin, Veronique De Smedt-Peyrusse, Fabien Ducrocq, Rim Baccouch, Asma Oummadi, Maria Hauge Pedersen, Brian Medel-Lacruz, Maria-Florencia Angelo, Sandrine Villette, Pierre Van Delft, Laetitia Fouillen, Sebastien Mongrand, Jana Selent, Tarson Tolentino-Cortez, Gabriel Barreda-Gomez, Stephane Gregoire, Elodie Masson, Thierry Durroux, Jonathan A. Javitch, Ramon Guixa-Gonzalez, Isabel D. Alves, Pierre Trifilieff
Summary: Increasing evidence suggests a connection between lipid metabolism and mental health, particularly in relation to polyunsaturated fatty acids (PUFAs). Recent findings show that PUFA levels in the brain are linked to dopamine transmission, a key system involved in psychiatric symptoms. However, the underlying mechanisms of this relationship are still unclear.
MOLECULAR PSYCHIATRY
(2023)
Article
Multidisciplinary Sciences
Carole Lartigue, Bastien Lambert, Fabien Rideau, Yorick Dahan, Marion Decossas, Melanie Hillion, Jean-Paul Douliez, Julie Hardouin, Olivier Lambert, Alain Blanchard, Laure Beven
Summary: Bacterial cell shape is determined by the interplay between the cell wall and cytoplasmic filaments made of MreB proteins. In this study, the heterologous expression of Spiroplasma fibril and MreB proteins in Mycoplasma cells, which are naturally spherical and non-motile, conferred helical shape and kinking ability. However, efficient motility in culture broth still requires unknown components from Spiroplasma.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Wiem Abidi, Marion Decossas, Lucia Torres-Sanchez, Lucie Puygrenier, Sylvie Letoffe, Jean-Marc Ghigo, Petya Krasteva
Summary: This study provides structural and mechanistic insights into the role of the accessory subunits BcsH and BcsD in determining crystalline BC secretion in the Gluconacetobacter lineage. The research reveals that oligomeric BcsH drives the assembly of BcsD into a supramolecular cytoskeletal scaffold, stabilizing the cellulose-extruding synthase nanoarrays through an unexpected inside-out mechanism for secretion system assembly.
Article
Cell Biology
Celine Gounou, Flora Bouvet, Benjamin Liet, Valerie Prouzet-Mauleon, Lena d'Agata, Etienne Harte, Francoise Argoul, Geraldine Siegfried, Richard Iggo, Abdel-Majid Khatib, Anthony Bouter
Summary: ANXA5 and ANXA6 are crucial components of the membrane repair machinery in MDA-MB-231 cells and are correlated with the invasiveness of breast cancer cells. High expression of ANXA5 and ANXA6 predicts poor prognosis in high-grade lung, gastric, and breast cancers. Genetic inhibition of ANXA5 and ANXA6 in zebrafish significantly reduces tumor cell dissemination.
BIOLOGY OF THE CELL
(2023)
Article
Biochemistry & Molecular Biology
Adiilah Mamode Cassim, Yotam Navon, Yu Gao, Marion Decossas, Laetitia Fouillen, Axelle Grelard, Minoru Nagano, Olivier Lambert, Delphine Bahammou, Pierre Van Delft, Lilly Maneta-Peyret, Francoise Simon-Plas, Laurent Heux, Bruno Jean, Giovanna Fragneto, Jenny C. Mortimer, Magali Deleu, Laurence Lins, Sebastien Mongrand
Summary: This study investigates the biological roles and properties of glycosyl inositol phosphoryl ceramides (GIPCs) in plant plasma membrane (PM). The results showed that GIPCs play important roles in regulating the thickness, electronegativity, and the gel-to-fluid phase transition of model membranes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Meeting Abstract
Biophysics
Rim Baccouch, Verronique de Smedt-Peyrusse, Marie-Lise Jobin, Thierry Durroux, David Perrais, Pierre Trifilieff, Isabel D. Alves
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)