High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging

Spatial distribution and function of T follicular regulatory cells in human lymph nodes

(2018) Ismail Sayin et al.   JOURNAL OF EXPERIMENTAL MEDICINE

A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing

(2018) Tatsuya C. Murakami et al.   NATURE NEUROSCIENCE

Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

(2018) Tsung-Li Liu et al.   SCIENCE

Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging

(2018) Yury Goltsev et al.   CELL

A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging

(2018) Leeat Keren et al.   CELL

Innate and adaptive lymphocytes sequentially shape the gut microbiota and lipid metabolism

(2018) Kairui Mao et al.   NATURE

Hyperactivated PI3Kδ promotes self and commensal reactivity at the expense of optimal humoral immunity

(2018) Silvia Preite et al.   NATURE IMMUNOLOGY

Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents

(2018) Kazuki Tainaka et al.   Cell Reports

High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization

(2017) Marcela Alcántara-Hernández et al.   IMMUNITY

Dendritic cell and antigen dispersal landscapes regulate T cell immunity

(2017) Michael Y. Gerner et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging

(2017) Francesco Cutrale et al.   NATURE METHODS

Multiplex, quantitative cellular analysis in large tissue volumes with clearing-enhanced 3D microscopy (Ce3D)

(2017) Weizhe Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Follicular CD8 T cells accumulate in HIV infection and can kill infected cells in vitro via bispecific antibodies

(2017) Constantinos Petrovas et al.   Science Translational Medicine

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution

(2017) Shimpei I. Kubota et al.   Cell Reports

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein–Protein Interaction Analysis, and Protein Function Exploration

(2017) Els Beghein et al.   Frontiers in Immunology

Chemical Principles in Tissue Clearing and Staining Protocols for Whole-Body Cell Profiling

(2016) Kazuki Tainaka et al.   Annual Review of Cell and Developmental Biology

Mass Cytometry: Single Cells, Many Features

(2016) Matthew H. Spitzer et al.   CELL

An improved method for isolation of epithelial and stromal cells from the human endometrium

(2016) Ayako MASUDA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Defining CD8+ T cells that provide the proliferative burst after PD-1 therapy

(2016) Se Jin Im et al.   NATURE

Shrinkage-mediated imaging of entire organs and organisms using uDISCO

(2016) Chenchen Pan et al.   NATURE METHODS

Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent

(2016) Meng-Tsen Ke et al.   Cell Reports

Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems

(2015) Evan Murray et al.   CELL

Clarifying Tissue Clearing

(2015) Douglas S. Richardson et al.   CELL

Quantifying Memory CD8 T Cells Reveals Regionalization of Immunosurveillance

(2015) Elizabeth M. Steinert et al.   CELL

Microbiota-Dependent Sequelae of Acute Infection Compromise Tissue-Specific Immunity

(2015) Denise Morais da Fonseca et al.   CELL

Strategically Localized Dendritic Cells Promote Rapid T Cell Responses to Lymph-Borne Particulate Antigens

(2015) Michael Y. Gerner et al.   IMMUNITY

Immune homeostasis enforced by co-localized effector and regulatory T cells

(2015) Zhiduo Liu et al.   NATURE

ScaleS: an optical clearing palette for biological imaging

(2015) Hiroshi Hama et al.   NATURE NEUROSCIENCE

Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method

(2015) Jia-Ren Lin et al.   Nature Communications

Lymph-Node Resident CD8α+ Dendritic Cells Capture Antigens from Migratory Malaria Sporozoites and Induce CD8+ T Cell Responses

(2015) Andrea J. Radtke et al.   PLoS Pathogens

Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing

(2014) Bin Yang et al.   CELL

iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging

(2014) Nicolas Renier et al.   CELL

Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization

(2014) Kazuki Tainaka et al.   CELL

ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue

(2013) T. Kuwajima et al.   DEVELOPMENT

Structural and molecular interrogation of intact biological systems

(2013) Kwanghun Chung et al.   NATURE

Influence of tumour micro-environment heterogeneity on therapeutic response

(2013) Melissa R. Junttila et al.   NATURE

SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction

(2013) Meng-Tsen Ke et al.   NATURE NEUROSCIENCE

Histo-Cytometry: A Method for Highly Multiplex Quantitative Tissue Imaging Analysis Applied to Dendritic Cell Subset Microanatomy in Lymph Nodes

(2012) Michael Y. Gerner et al.   IMMUNITY

CD4 T follicular helper cell dynamics during SIV infection

(2012) Constantinos Petrovas et al.   JOURNAL OF CLINICAL INVESTIGATION

Three-dimensional imaging of solvent-cleared organs using 3DISCO

(2012) Ali Ertürk et al.   Nature Protocols

RNAscope

(2011) Fay Wang et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

(2011) Hiroshi Hama et al.   NATURE NEUROSCIENCE

Cellular Heterogeneity: Do Differences Make a Difference?

(2010) Steven J. Altschuler et al.   CELL