期刊
BIOMATERIALS
卷 203, 期 -, 页码 86-95出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2019.01.038
关键词
Mesenchymal stem cells; Peripheral nerve repair; Nerve conduit; Wall encapsulation; Schwann cell distribution
资金
- U.S. Department of Defense [W81XWH-15-1-0600]
- NIH [T32 EB001026]
An essential structure in nerve regeneration within engineered conduits is the nerve bridge initiated by centrally migrating Schwann cells in response to chemokine gradients. Introducing exogenous cells secreting neurotrophic factors aims to augment this repair process, but conventional cell-seeding methods fail to produce a directional chemokine gradient. We report a versatile method to encapsulate cells within conduit walls, allowing for reproducible control of spatial distribution along the conduit. Conduits with stem cells encapsulated within the central third possessed markedly different cell distribution and retention over 6 weeks in vivo, compared to standard cell lumen injection. Such a construct promoted Schwann cell migration centrally, and at 16 weeks rats presented with significantly enhanced function and axonal myelination. The method of utilizing a spatially restricted cell secretome departs from traditional homogeneous cell loading, and presents new approaches for studying and maximizing the potential of cell application in peripheral nerve repair.
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