Review
Pharmacology & Pharmacy
Prabhsimran Kaur, Bidwan Sekhar Behera, Sandeep Singh, Anjana Munshi
Summary: Diabetes, a major cause of death worldwide, is currently treated with various strategies that often come with side effects. The new approach of SGLT2 inhibition shows promising results in controlling glucose levels with minimal adverse effects.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jessica Wojciechowski, Bimal K. Malhotra, Xiaoxing Wang, Luke Fostvedt, Hernan Valdez, Timothy Nicholas
Summary: This study provides insights into the impact of Abrocitinib treatment on platelet counts and identifies factors affecting platelet reduction. Overall, the findings support the safety of Abrocitinib and suggest a low probability of thrombocytopenia during treatment.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Robert F. Kelley, Devin B. Tesar, Yue Wang, Nicholas J. Agard, Patrick G. Holder, Joyce Chan, Laetitia Comps-Agrar, Judit Horvath, Joshua D. Horvath, Susan R. Crowell
Summary: Researchers have successfully developed a stable surrogate molecule for testing ocular drug delivery systems in a porcine model, which has low porcine immunogenicity and can continuously release the drug.
MOLECULAR PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Vivaswath S. Ayyar, Pharavee Jaiprasart, Brian Geist, Zheng Huang Devine, Martin Case, Anasuya Hazra, Chyi-Hung Hsu, Madhu Chintala, Weirong Wang
Summary: The study developed antigen-binding fragment (F-ab) reversal agents to reverse the anticoagulant effect of JNJ-64179375 (JNJ-9375) in bleeding emergencies. Three reversal agents of varying binding affinities to JNJ-9375 were tested in cynomolgus monkeys, showing different neutralization characteristics in vivo. The model-based approach helped select JNJ-67842125 as the most effective reversal agent for JNJ-9375 neutralization.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Hematology
Joanne L. Dunster, Alexander P. Bye, Neline Kriek, Tanya Sage, Joanne L. Mitchell, Carly Kempster, Joana Batista, Harriet McKinney, Patrick Thomas, Chris Jones, Kate Downes, Amanda J. Unsworth, Jonathan M. Gibbins
Summary: The PPAnalysis assay provides a detailed characterization of platelet function and identifies distinct subpopulations of donors based on their platelet reactivity metrics. It shows that platelet reactivity is not simply categorized as high or low responders, but exists in 6 subgroups with distinct platelet phenotypes within healthy cohorts. These platelet phenotypes are stable and can be used for stratification of cohorts to investigate the causes and consequences of differences in platelet function.
Review
Pharmacology & Pharmacy
Stefano Fogli, Sara Galimberti, Veronica Gori, Marzia Del Re, Romano Danesi
Summary: This review article explores the differences in potency, selectivity, pharmacokinetics, safety, and drug-drug interactions of clinically validated proteasome inhibitors, aiming to provide useful information for their clinical use in real life settings.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Medicine, Research & Experimental
Cheng Zhang, Jordan W. Bye, Lok H. Lui, Hongyu Zhang, John Hales, Steve Brocchini, Robin A. Curtis, Paul A. Dalby
Summary: This study aimed to investigate the effect of protein concentration on the aggregation of protein therapeutics, using the antibody Fab fragment A33 as a model protein. The results showed that increasing the concentration led to a decrease in the relative aggregation rate, but an increase in the absolute aggregation rate. The higher concentration also resulted in a more stable protein structure with reduced conformational flexibility. Surface adsorption, diffusion limitations, and simple volume crowding were found to have no significant effect on the aggregation rate. The kinetic data suggested a reversible two-state conformational switch mechanism at higher concentrations.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Boram Gu, Yu Huang, Emily Louise Manchester, Alun D. Hughes, Simon A. McG Thom, Rongjun Chen, Xiao Yun Xu
Summary: This study establishes a multiphysics simulation platform for evaluating the effects of therapeutic parameters on thrombolysis dynamics and side effects. Results show that tPA-NV offers advantages in reducing bleeding risk, lowering tPA dosage, and accelerating recanalisation, making it a potential new thrombolytic strategy.
PHARMACEUTICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Cheng Zhang, Nuria Codina, Jiazhi Tang, Haoran Yu, Nesrine Chakroun, Frank Kozielski, Paul A. Dalby
Summary: The research findings highlighted the different destabilizing pathways of protein structure under low pH and high temperature stress conditions, revealing the potential for stabilizing mutations in the CL domain and C-L-C(H)1 interface. The analysis also identified key salt bridge losses as a cause of conformational changes under different stresses, with implications for future engineering efforts to enhance stability.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Luke F. Pennington, Pascal Gasser, Silke Kleinboelting, Chensong Zhang, Georgios Skiniotis, Alexander Eggel, Theodore S. Jardetzky
Summary: The therapeutic effects of antibody drugs can be enhanced through the mechanism of facilitated dissociation, where disruption of high-affinity complexes by antibodies can potentially increase efficacy. The authors developed a yeast display approach to select and engineer omalizumab variants that dissociate receptor-bound IgE, providing insights for engineering disruptive inhibitors for other targets and improving the inhibition of allergic responses.
NATURE COMMUNICATIONS
(2021)
Review
Chemistry, Medicinal
Luigi Principe, Tommaso Lupia, Lilia Andriani, Floriana Campanile, Davide Carcione, Silvia Corcione, Francesco Giuseppe De Rosa, Roberto Luzzati, Giacomo Stroffolini, Marina Steyde, Giuliana Decorti, Stefano Di Bella
Summary: Bacterial resistance mechanisms are continuously and rapidly evolving, especially for Gram-negative bacteria. The strategy of developing new beta-lactam/beta-lactamase inhibitors (BLs/BLIs) combinations has been successful, and data on several compounds are becoming available. Cefiderocol, with its unique mechanism of action, warrants separate discussion.
Article
Pharmacology & Pharmacy
Bing Wang, Jo Goodman, Lorin K. Roskos
Summary: Therapeutic IgG(4) antibodies undergo Fab-arm exchange with endogenous IgG(4) to form monovalent hybrid molecules. This exchange is observed with tralokinumab, a monoclonal antibody being developed to treat atopic dermatitis, in healthy volunteers. The formation of monovalent hybrids becomes the dominant form of tralokinumab in volunteers within 1 day postdose, but it does not affect the potency of neutralizing interleukin-13.
CPT-PHARMACOMETRICS & SYSTEMS PHARMACOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Anne H. Tavenier, Renicus S. Hermanides, Enrico Fabris, Dominick J. Angiolillo, Arnoud W. J. van't Hof
Summary: Various strategies have been proposed to bridge the gap in platelet inhibition in STEMI patients undergoing primary PCI, aiming to optimize clinical outcomes in the acute phase.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Multidisciplinary Sciences
Amin Polzin, Lisa Dannenberg, Rene M'Pembele, Philipp Mourikis, David Naguib, Saif Zako, Carolin Helten, Tobias Petzold, Bodo Levkau, Thomas Hohlfeld, Mareike Barth, Tobias Zeus, Stephan Sixt, Ragnar Huhn, Payam Akhyari, Artur Lichtenberg, Malte Kelm, Till Hoffmann
Summary: In patients with infective endocarditis, platelet reactivity is increased in those with Staphylococcus aureus (SA) infection. However, the use of aspirin can reduce this increased reactivity without increasing bleeding risk. More research is needed to confirm the potential benefits of aspirin in SA-positive infective endocarditis.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Francisco Gomez, Jesyree Veita, Krzysztof Laudanski
Summary: Extracorporeal membrane oxygenation (ECMO) is a treatment modality that requires frequent use of antibiotics. However, there are several variables that can affect the success of antibiotic therapy during ECMO implementation, including changes in drug distribution, clearance, adsorption into circuit components, and complex interactions with critical care illnesses. Additionally, ECMO complicates the assessment of antibiotic effectiveness due to difficulties in detecting fever or other signs, and the immunogenicity of the circuit can affect biomarkers and disrupt the immune system.
Article
Cell Biology
Hari Hara Sudhan Lakshmanan, Alexander R. Melrose, Anna-Liisa Sepp, Annachiara Mitrugno, Anh T. P. Ngo, Ayesha Khader, Rachel Thompson, Daniel Sallee, Jiaqing Pang, Pierre H. Mangin, Martine Jandrot-Perrus, Joseph E. Aslan, Owen J. T. McCarty
Summary: In this study, it was demonstrated that recombinant human nidogen-1 supports platelet adhesion and activates platelets in a kinase-dependent manner downstream. Platelet adhesion to nidogen-1 was found to activate the IKK complex, while pharmacological inhibition of IKK blocked platelet spreading on nidogen. Overall, these results suggest that nidogen may play a redundant role in hemostasis by activating platelets downstream of GPVI.
Article
Food Science & Technology
Sabrina Karim-Silva, Alessandra Becker-Finco, Isabella Gizzi Jiacomini, Fanny Boursin, Arnaud Leroy, Magali Noiray, Juliana de Moura, Nicolas Aubrey, Philippe Billiald, Larissa M. Alvarenga
Article
Oncology
Dorothee Faille, Lamia Lamrani, Stephane Loyau, Marie-Genevieve Huisse, Marie-Charlotte Bourrienne, Sawsaneh Alkhaier, Bruno Cassinat, Yacine Boulaftali, Jerome Debus, Martine Jandrot-Perrus, Christine Chomienne, Christine Dosquet, Nadine Ajzenberg
Article
Hematology
Muhammad Usman Ahmed, Valeria Kaneva, Stephane Loyau, Dmitry Nechipurenko, Nicolas Receveur, Marion Le Bris, Emily Janus-Bell, Melusine Didelot, Antoine Rauch, Sophie Susen, Nabil Chakfe, Francois Lanza, Elizabeth E. Gardiner, Robert K. Andrews, Mikhail Panteleev, Christian Gachet, Martine Jandrot-Perrus, Pierre H. Mangin
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Article
Pharmacology & Pharmacy
Lionel Renaud, Kristell Lebozec, Christine Voors-Pette, Peter Dogterom, Philippe Billiald, Martine Jandrot Perrus, Yannick Pletan, Matthias Machacek
JOURNAL OF CLINICAL PHARMACOLOGY
(2020)
Article
Cell Biology
Stephane Loyau Inserm, Dorothee Faille, Philippe Gautier, Paquita Nurden, Martine Jandrot-Perrus, Nadine Ajzenberg
Summary: Acquired deficiencies in platelet glycoprotein VI are rare and usually associated with immune thrombocytopenia. This case report describes a male patient with an immune GPVI deficiency linked to a mutation in the alpha-actinin gene, who was successfully treated with dual antiplatelet therapy without any bleeding complications.
Article
Hematology
Gina Perrella, Jingnan Huang, Isabella Provenzale, Frauke Swieringa, Floor C. J. Heubel-Moenen, Richard W. Farndale, Mark Roest, Paola E. J. van der Meijden, Mark Thomas, Robert A. S. Ariens, Martine Jandrot-Perrus, Steve P. Watson, Johan W. M. Heemskerk
Summary: The study revealed that fibrin fibers play a consolidating role in microthrombus formation through nonredundant signaling of platelet glycoprotein VI and integrin alpha IIb beta 3 via Syk and low-level Ca2+ rises, even in the absence of thrombin, leading to minimal thrombin generation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Letter
Hematology
Soumaya Jadoui, Ophelie Le Chapelain, Veronique Ollivier, Ali Mostefa-Kara, Lucas Di Meglio, Sebastien Dupont, Angele Gros, Mialitiana Solo Nomenjanahary, Jean-Philippe Desilles, Mikael Mazighi, Bernhard Nieswandt, Stephane Loyau, Martine Jandrot-Perrus, Pierre H. Mangin, Benoit Ho-Tin-Noe
Article
Cell Biology
Francois Delvoye, Stephane Loyau, Julien Labreuche, Guillaume Taylor, Benjamin Maier, Michel Piotin, Raphael Blanc, Simon Escalard, Lucas Di Meglio, Malek Ben Maacha, Hocine Redjem, Stanislas Smajda, Gabriele Ciccio, Solene Hebert, Candice Sabben, Martine Jandrot-Perrus, Alain Maertens De Noordhout, Mikael Mazighi, Benoit Ho-Tin-Noe, Jean-Philippe Desilles
Summary: This study investigated the use of the glycoprotein IIb/IIIa antagonist abciximab in treating immediate reocclusion after mechanical thrombectomy for acute ischemic stroke. The results showed that abciximab treatment led to higher recanalization rates without increasing bleeding complications. In vitro experiments further demonstrated that abciximab effectively limits thrombus accretion by blocking platelet aggregation.
Editorial Material
Hematology
Alexandre Slater, Martine Jandrot-Perrus
Summary: The authors of this study report the structure of platelet glycoprotein VI complexed with collagen-related peptides and propose the structural basis of GPVI clustering on collagen fibers.
Article
Biochemistry & Molecular Biology
Isabella Gizzi Jiacomini, Martina Beltramino, Fanny Boursin, Joao Carlos Minozzo, Juliana Ferreira de Moura, Philippe Billiald, Larissa Magalhaes Alvarenga, Nicolas Aubrey
Summary: The use of monoclonal antibodies in therapy is expanding, with focus on safety, cost-effectiveness, and efficacy. Recombinant DNA technology is used for large-scale production, and strategies for humanization have been developed considering their heterologous origin and potential immunogenicity. This study introduced a mouse monoclonal antibody with binding and neutralizing abilities against spider venom toxins, and explored its humanization potential.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Ilaria De Simone, Constance C. F. M. J. Baaten, Martine Jandrot-Perrus, Jonathan M. Gibbins, Hugo ten Cate, Johan W. M. Heemskerk, Chris Jones, Paola E. J. van der Meijden
Summary: Platelet and coagulation activation are reciprocal processes, and factors other than thrombin or fibrin can induce platelet activation via GPVI and PAR receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Philippe Billiald, Alexandre Slater, Martin Welin, Joanne C. Clark, Stephane Loyau, Martine Pugniere, Isabella G. Jiacomini, Nadia Rose, Kristell Lebozec, Elie Toledano, Deborah Francois, Steve P. Watson, Martine Jandrot-Perrus
Summary: This study shows that glenzocimab, a humanized antibody fragment, blocks the binding of GPVI to vascular collagen and fibrin through steric hindrance and structural change. This finding is significant for the development of new antiplatelet molecules with a low bleeding risk.
Letter
Hematology
Emily Janus-Bell, Muhammad Usman Ahmed, Nicolas Receveur, Clarisse Mouriaux, Bernhard Nieswandt, Elizabeth E. Gardiner, Christian Gachet, Martine Jandrot-Perrus, Pierre H. Mangin
THROMBOSIS AND HAEMOSTASIS
(2021)