Following the Evolutionary Track of a Highly Specific L-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes

Benchmark Analysis of Native and Artificial NAD+-Dependent Enzymes Generated by a Sequence-Based Design Method with or without Phylogenetic Data

(2018) Shogo Nakano et al.   BIOCHEMISTRY

Structure and Enzymatic Properties of an Unusual Cysteine Tryptophylquinone-Dependent Glycine Oxidase from Pseudoalteromonas luteoviolacea

(2018) Andres Andreo-Vidal et al.   BIOCHEMISTRY

Evolution of chalcone isomerase from a noncatalytic ancestor

(2018) Miriam Kaltenbach et al.   Nature Chemical Biology

Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein

(2018) Ben E. Clifton et al.   Nature Chemical Biology

Recombinant expression and characterization of a l-amino acid oxidase from the fungus Rhizoctonia solani

(2016) Katharina Hahn et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Exploring the past and the future of protein evolution with ancestral sequence reconstruction: the 'retro approach to protein engineering

(2016) Y. Gumulya et al.   BIOCHEMICAL JOURNAL

Evolution of Enzyme Superfamilies: Comprehensive Exploration of Sequence–Function Relationships

(2016) F. Baier et al.   BIOCHEMISTRY

Ancestral protein reconstruction: techniques and applications

(2016) Rainer Merkl et al.   BIOLOGICAL CHEMISTRY

l -Arginine oxidase from Pseudomonas sp. TPU 7192: Characterization, gene cloning, heterologous expression, and application to l -arginine determination

(2016) Daisuke Matsui et al.   ENZYME AND MICROBIAL TECHNOLOGY

Roles of Conserved Residues of the Glycine Oxidase GoxA in Controlling Activity, Cooperativity, Subunit Composition, and Cysteine Tryptophylquinone Biosynthesis

(2016) Esha Sehanobish et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases

(2016) Titu Devamani et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

On the Potential Origins of the High Stability of Reconstructed Ancestral Proteins

(2016) Devin L. Trudeau et al.   MOLECULAR BIOLOGY AND EVOLUTION

Consensus protein design

(2016) Benjamin T. Porebski et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Evolutionary drivers of thermoadaptation in enzyme catalysis

(2016) Vy Nguyen et al.   SCIENCE

An experimental phylogeny to benchmark ancestral sequence reconstruction

(2016) Ryan N. Randall et al.   Nature Communications

Heterologous production ofl-lysine ε-oxidase by directed evolution using a fusion reporter method

(2015) Daisuke Matsui et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

l-Amino acid oxidase as biocatalyst: a dream too far?

(2013) Loredano Pollegioni et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

X-Ray crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in l-lysine ε-oxidase from Marinomonas mediterranea

(2013) Seiji Okazaki et al.   JOURNAL OF BIOCHEMISTRY

Hyperstability and Substrate Promiscuity in Laboratory Resurrections of Precambrian β-Lactamases

(2013) Valeria A. Risso et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0

(2013) Koichiro Tamura et al.   MOLECULAR BIOLOGY AND EVOLUTION

Experimental evidence for the thermophilicity of ancestral life

(2013) S. Akanuma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Arg305 of Streptomyces l-glutamate oxidase plays a crucial role for substrate recognition

(2011) Tomohiro Utsumi et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

L-Amino acid oxidase of the fungus Hebeloma cylindrosporum displays substrate preference towards glutamate

(2011) J. T. Nuutinen et al.   MICROBIOLOGY-SGM

HMMER web server: interactive sequence similarity searching

(2011) R. D. Finn et al.   NUCLEIC ACIDS RESEARCH

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

O2Reactivity of Flavoproteins

(2010) Jan Saam et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Consensus Protein Design without Phylogenetic Bias

(2010) Christian Jäckel et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural characterization of l-glutamate oxidase fromStreptomycessp. X-119-6

(2009) Jiro Arima et al.   FEBS Journal

The macromolecule with antimicrobial activity synthesized by Pseudoalteromonas luteoviolacea strains is an l-amino acid oxidase

(2008) Daniel Gómez et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Structural Basis of Proteolytic Activation of l-Phenylalanine Oxidase fromPseudomonassp. P-501

(2008) Koh Ida et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Palaeotemperature trend for Precambrian life inferred from resurrected proteins

(2008) Eric A. Gaucher et al.   NATURE