Article
Plant Sciences
Jingsong Guo, Sibo Wang, Ruixue Chen, Yu Guo, Jing Han, Guangli Li, Yushuang Guo, Wei Ji
Summary: The SUMO protease GmOTSa was identified in soybean and shown to have SUMO protease activity in the nucleus. Overexpression of GmOTSa conferred drought tolerance in tobacco plants by regulating ROS scavenging, reducing MDA content, increasing chlorophyll content, and promoting stomatal closure.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Review
Agriculture, Multidisciplinary
Zihan Li, Fan Zhong, Jianrong Guo, Zhuo Chen, Jie Song, Yi Zhang
Summary: Salinity is a major abiotic stress that affects crop yield and food supply in saline soil areas. Wheat, being the main crop in these areas, has developed various strategies to enhance salt tolerance through conventional breeding approaches and new techniques such as gene identification, gene stacking, and utilization of wild relatives.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Patricia V. Miranda, Bernardo F. Iglesias, Maria V. Charriere, Moises Burachik
Summary: The expression of HAHB4 sunflower transcription factor in wheat enhances drought tolerance. A feeding study on broilers showed that wheat event IND-empty setempty set412-7 had nutritional similarity with conventional wheat. The study also found no significant differences between wheat event IND-empty setempty set412-7 and its non-GM counterpart, supporting their nutritional equivalence.
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN
(2022)
Article
Biochemistry & Molecular Biology
Xiufang Liu, Qian Lu, Han Xiao, Yunzi Feng, Guowan Su, Mouming Zhao, Mingtao Huang
Summary: A salt-tolerant pseudolysin of Pseudomonas aeruginosa SWJSS3 isolated from deep-sea mud was expressed in Saccharomyces cerevisiae for potential applications in the detergent industry, the production of diagnostic kits, and traditional food fermentation. The proteolytic activity of the recombinant pseudolysin was significantly improved after optimizing the concentration of ion cofactors in the culture medium, and it showed high stability and activity under high-salt conditions.
Article
Biochemistry & Molecular Biology
Andrea Romero-Reyes, Sergio G. Hernandez-Leon, Lilia Leyva-Carrillo, Gloria Yepiz-Plascencia, Matthew P. Reynolds, Matthew J. Paul, Sigrid Heuer, Elisa M. Valenzuela-Soto
Summary: High temperatures hinder high-yield production in bread wheat by negatively impacting photosynthesis. This study aimed to investigate the photosynthetic performance of 10 bread wheat genotypes under high temperatures. The results showed a significant reduction in yield under heat stress, but no impact on chlorophyll content and light-dependent reactions. Three genotypes exhibited heat tolerance and could be potential candidates for breeding heat-tolerant bread wheat in this region.
BIOCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Yongang Yu, Yanxia Wu, Lingyun He
Summary: The WRKY transcription factor TaWRKY17 plays a crucial role in enhancing salt tolerance in wheat by regulating ABA/ROS-related, and stress-response genes, and increasing anti-oxidative stress capabilities.
PLANT MOLECULAR BIOLOGY
(2023)
Review
Biochemical Research Methods
Radheshyam Yadav, Shivam Chaudhary, Wusirika Ramakrishna
Summary: This review explores the role of SUMOylation in plant response to abiotic stresses and its potential contribution to plant stress tolerance. It also discusses the potential of SUMOylation in other biological processes.
PLANT MOLECULAR BIOLOGY REPORTER
(2023)
Article
Agronomy
Hafid Aberkane, Bouchra Belkadi, Zakaria Kehel, Abdelkarim Filali-Maltouf, Izzat S. A. Tahir, Sara Meheesi, Ahmed Amri
Summary: The study investigated the effects of drought and chronic heat stress on physiological parameters of durum wheat lines derived from interspecific crosses and their association with yield. Physiological parameters like canopy temperature (CT) and maximum quantum yield of PSII (Fv/Fm) showed significant correlations with grain yield and biomass under heat and drought stress, providing potential indicators for selecting wheat varieties with tolerance to these abiotic stresses.
Article
Multidisciplinary Sciences
Kavita Lamba, Mukesh Kumar, Vikram Singh, Lakshmi Chaudhary, Rajat Sharma, Shikha Yashveer, M. S. Dalal
Summary: This study evaluated the heat stress tolerance of 50 wheat genotypes to improve stress tolerance. The results showed a negative correlation between tolerance index and yield, and a positive correlation between stress tolerance index, mean productivity, and other indices with yield. Several suitable and high-yielding genotypes for cultivation in high temperature conditions were identified based on principal component, biplot, and cluster analysis.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Muhammad Adrees, Zahra Saeed Khan, Muhammad Hafeez, Muhammad Rizwan, Khalid Hussain, Muhammad Asrar, Mohammed Nasser Alyemeni, Leonard Wijaya, Shafaqat Ali
Summary: The study showed that ZnO NPs can promote the growth of wheat, with the best results seen at 100 mg/L under normal moisture conditions. In grains, the Cd concentrations decreased by 26%, 81%, and 87% when using 25, 50, and 100 mg/L NPs, respectively, under normal moisture conditions, and decreased by 35%, 66%, and 81% under water deficit conditions.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Genetics & Heredity
Bin Yang, Xiaojie Wen, Hongwei Wen, Yanru Feng, Jiajia Zhao, Bangbang Wu, Xingwei Zheng, Chenkang Yang, Sanwei Yang, Ling Qiao, Jun Zheng
Summary: In this study, a recombinant inbred line (RIL) population was constructed from a cross of drought-sensitive and drought-resistant wheat varieties to analyze 29 QTLs controlling flag leaf chlorophyll content. The major QTLs detected under different water regimes provide valuable information for understanding the genetic mechanism underlying chlorophyll content and molecular marker-assisted breeding.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Muhammad Adil, Safdar Bashir, Saqib Bashir, Zubair Aslam, Niaz Ahmad, Tasaddaq Younas, Rana Muhammad Ammar Asghar, Jawaher Alkahtani, Yheni Dwiningsih, Mohamed S. Elshikh
Summary: Nanotechnology has a wide range of applications in agriculture, and zinc oxide nanoparticles (ZnO NPs) have been found to improve the growth and yield of wheat under salt stress conditions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Xinran Cheng, Hui Yao, Zuming Cheng, Bingbing Tian, Chang Gao, Wei Gao, Shengnan Yan, Jiajia Cao, Xu Pan, Jie Lu, Chuanxi Ma, Cheng Chang, Haiping Zhang
Summary: This study evaluated the effect of the gene TaVQ14 on salt and drought stress resistance in wheat. The results showed that TaVQ14 could enhance the resistance of Arabidopsis seeds to salt and drought stress by scavenging reactive oxygen species and enhancing calcium signaling.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Junjie Han, Yichen Liu, Yiting Shen, Weihua Li
Summary: Drought is a major limiting factor for wheat productivity, and it is expected to have an increasing negative impact on crop growth in arid areas due to climate deterioration. In this study, the wheat TaXTH gene family was studied, and it was found that TaXTH genes play a role in regulating drought response in wheat. The expression of TaXTH12.5a enhanced drought tolerance in Arabidopsis, further supporting the role of XTH genes in regulating drought stress response in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Jun Chen, Yan Gong, Yuan Gao, Yongbin Zhou, Ming Chen, Zhaoshi Xu, Changhong Guo, Youzhi Ma
Summary: In this study, 43 putative drought-induced NAC genes were identified in wheat, with TaNAC48 showing significantly increased expression under drought and promoting drought tolerance through crosstalk with the ABA signaling pathway. These results suggest an essential role of TaNAC48 in mediating drought stress responses in wheat.
Article
Plant Sciences
Marlon-Schylor L. le Roux, N. Francois Burger, Mare Vlok, Karl J. Kunert, Christopher A. Cullis, Anna-Maria Botha
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Agronomy
Maria Lorena Sereno, Sofia Infante, Adriana Cheavegatti-Gianotto, Kyra Hjelle, Ronald Lirette, Lucas Cutri, Moises Sarto Rocha, Jerry Hjelle, Christopher Cullis
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN
(2020)
Article
Biochemistry & Molecular Biology
Marlon-Schylor L. le Roux, Nicolas Francois V. Burger, Mare Vlok, Karl J. Kunert, Christopher A. Cullis, Anna-Maria Botha
Summary: This study characterizes a new mutant BIG8-1 with superior drought response characteristics, showcasing unique phenotypic traits and exceptional metabolic activity under severe water deficit stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Coenraad R. van Beek, Tapiwa Guzha, Nolusindiso Kopana, Cornelius S. van der Westhuizen, Sanjib K. Panda, Christell van der Vyver
Summary: The study evaluated the effects of overexpressing SlNAC2 TF on tobacco plants under water-deficit stress, showing that the transgenic plants maintained higher relative water content and chlorophyll levels, increased proline levels, improved seed setting, and delayed leaf senescence. The overexpression of SlNAC2 also resulted in lower levels of reactive oxygen species in dehydrated plants, without significant differences in superoxide dismutase and catalase content, but with increased glutathione reductase transcript levels.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Plant Sciences
Jin Li, Christopher Cullis
Summary: The marama bean, a wild legume from tropical Africa, is being considered as a potential crop for local farmers due to its nutritional value. Research has shown that the mitochondrial genome of marama bean is 399,572 bp in length, with a 9,798 bp DNA fragment homologous to the chloroplast genome. Compared to other legumes, certain genes in the mitochondrial genome of marama have remained intact and functional throughout evolution.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Casey Gabriel, Jean-Jacque Fernhout, Franziska Fichtner, Regina Feil, John E. Lunn, Jens Kossmann, James R. Lloyd, Christell Van der Vyver
Summary: Manipulation of Tre6P/trehalose metabolism can alter the profile of soluble sugars accumulated in sugarcane stems. Overexpressing otsA and otsB genes or silencing native TPS expression in sugarcane led to changes in sucrose levels and the accumulation of other sugars in different tissues of the plant.
Article
Biochemical Research Methods
Carolyn J. Lawrence-Dill, Robyn L. Allscheid, Albert Boaitey, Todd Bauman, Edward S. Buckler, Jennifer L. Clarke, Christopher Cullis, Jack Dekkers, Cassandra J. Dorius, Shawn F. Dorius, David Ertl, Matthew Homann, Guiping Hu, Mary Losch, Eric Lyons, Brenda Murdoch, Zahra-Katy Navabi, Somashekhar Punnuri, Fahad Rafiq, James M. Reecy, Patrick S. Schnable, Nicole M. Scott, Moira Sheehan, Xavier Sirault, Margaret Staton, Christopher K. Tuggle, Alison Van Eenennaam, Rachael Voas
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Agronomy
Christopher Cullis, Percy Chimwamurombe, Karl Kunert, Juan Vorster
Summary: Recent attention has been given to indigenous knowledge of plant species that have been consumed by humans but not actively cultivated in organized agriculture. This article focuses on the marama bean, an orphan plant that has not been grown as a crop but has potential for wider agricultural use. The authors discuss the current status of marama bean domestication and identify possible pathways for its integration into formal agriculture. They emphasize the need for scientific improvement, added value characteristics, and the involvement of growers in this process. The domestication of marama bean could also benefit other legume crops and contribute to food security in arid regions.
FOOD AND ENERGY SECURITY
(2023)
Article
Agriculture, Multidisciplinary
M. Masoabi, S. Snyman, C. Van der Vyver
Summary: A mutant line, M9.2, was generated from a drought susceptible sugarcane cultivar and showed improved drought tolerance compared to the parental genotype. The mutant plants displayed higher photosynthetic efficiency, increased stomatal conductance, accumulation of proline, and enhanced antioxidant enzymes under water deficit stress. Differentially expressed proteins related to photosynthesis, carbohydrate metabolism, energy metabolism, methylation homeostasis, and DNA repair were observed. This study characterizes the new M9.2 mutant as a potential valuable resource for future sugarcane breeding programs.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Plant Sciences
Jin Li, Christopher Cullis
Summary: The chloroplast genomes of 84 marama samples from southern Africa were compared, revealing that certain regions of the marama plastome were more diverse. 15 haplotypes were identified, divided into two groups with differences at specific loci and an inversion. Furthermore, it was found that most individuals had both types of chloroplast genomes, albeit at a low frequency. The inheritance and coexistence of these complex chloroplast genomes raised questions for further research.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
M. Masoabi, N. F. V. Burger, A. -M. Botha, M. L. Le Roux, M. Vlok, S. Snyman, C. Van der Vyver
Summary: In this study, sugarcane was genetically transformed with the OTS1 gene from Arabidopsis thaliana to enhance drought tolerance. The transgenic plants showed improved drought tolerance and delayed leaf senescence, maintaining high moisture content and photosynthesis rate under water deficit. Additionally, they were able to maintain higher stomatal conductance and chlorophyll content under moderate stress and exhibited reduced oxidative damage under severe water deficit. The SUMOylation of proteins and protease activity were lower in the transgenic plants, suggesting that SUMO-related post-translational modifications played a role in enhancing drought tolerance in sugarcane.
Article
Plant Sciences
M. Masoabi, S. Snyman, S. Pols, P. N. Hills, C. van der Vyver
Summary: In this study, the response of sugarcane plants to water deficit stress was investigated by manipulating cytokinin levels. The results showed that the manipulation of cytokinin levels positively affected the plant's response to water deficit stress through various physiological mechanisms.
Article
Biochemical Research Methods
Nelisa Mbambalala, Sanjib K. Panda, Christell van der Vyver
Summary: B-box proteins play crucial roles in controlling growth, development, and response to stresses in plants. Overexpression of the AtBBX29 gene in sugarcane resulted in enhanced drought tolerance, delayed senescence, and improved physiological and biochemical mechanisms, making it a potential target for genetic engineering of drought tolerance in sugarcane.
PLANT MOLECULAR BIOLOGY REPORTER
(2021)