Article
Biochemistry & Molecular Biology
Ilda D'Annessa, Elisabetta Moroni, Giorgio Colombo
Summary: The Hsp90 chaperone system forms dynamic multiprotein complexes with a wide range of client proteins, involving cochaperone partners to regulate client activities, protect them from degradation, and respond to biochemical signals. Cryo-electron microscopy has provided a molecular picture of Hsp90 in complex with a kinase and a cochaperone. Structural modulations dependent on nucleotides lead to asymmetry in Hsp90 dynamics, revealing specific long-range coordination patterns between nucleotide binding, client binding, cochaperone, and client regions.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Shannon McMahon, Steven Bergink, Harm H. Kampinga, Heath Ecroyd
Summary: The study shows that DNAJB proteins can inhibit protein aggregation by supporting the Hsp70-dependent degradation pathway, different from the mechanism of preventing fibrillar aggregation. The research also found that DNAJB6 and DNAJB8 have two distinct regions for binding substrates.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Noeli Soares Melo Silva, Luiz Fernando de Camargo Rodrigues, Paulo Roberto Dores-Silva, Carlos Alberto Montanari, Carlos Henrique Inacio Ramos, Leandro Ramos Souza Barbosa, Julio Cesar Borges
Summary: The study reveals that rHSPA8 shares structural and functional similarities with other human Hsp70s, interacts with adenosine nucleotides, has lower ATPase activity compared to HSPA5/hGrp78/hBiP but higher than recombinant HSPA1A/hHsp70-1A, and shows capacity in preventing the aggregation of client proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Francesca A. Vaccaro, David A. Born, Catherine L. Drennan
Summary: G-protein metallochaperone MeaB facilitates the delivery of AdoCbl to MCM and genetic defects in MMAA lead to methylmalonic aciduria. The crystal structure of MeaB bound to a GTP analog GMPPCP with the Cbl-binding domain of MeMCMcbl reveals the activation mechanism of MeaB by target protein binding and the rearrangement of switch III region. These results provide insights into the molecular basis of AdoCbl delivery and the role of GTP hydrolysis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Sabina Kedzierska-Mieszkowska, Michal Zolkiewski
Summary: ClpB, a molecular chaperone in pathogenic bacteria, plays a crucial role in their survival and infection under host-induced stress, making it a promising target for novel antimicrobial therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sebastien Alphonse, Imane Djemil, Andrea Piserchio, Ranajeet Ghose
Summary: Bacterial tyrosine kinases (BY-kinases) and their counteracting phosphatases regulate bacterial synthesis and export processes. The structure of the complex between the BY-kinase Wzc and phosphatase Wzb has been determined, providing a mechanistic understanding of the phosphorylation-coupled assembly/disassembly process involved in BY-kinase function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Erin E. Deans, Judy L. M. Kotler, Wei-Shao Wei, Timothy O. Street
Summary: This study investigates the interaction between Hsp70 chaperones and client protein proIGF2, revealing that electrostatic attraction plays a crucial role in facilitating the recognition of oligomers.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Konstantin O. Muranov, Nicolay B. Poliansky, Vera A. Borzova, Sergey Y. Kleimenov
Summary: aH-Crystallin, a high molecular weight form of α-crystallin, plays a significant role in the development of cataracts. Refolding with urea increases the chaperone-like activity of α-crystallin and HMWA, while reducing their sizes. The chaperone-like activity of a-crystallin and HMWA correlates with denaturation enthalpy, suggesting a correction of misfolded proteins and rearrangement of protein structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sofia Platova, Liudmila Poliushkevich, Milana Kulakova, Maksim Nesterenko, Viktor Starunov, Elena Novikova
Summary: Hedgehog signaling is a crucial regulator of morphogenesis, cell differentiation, and regeneration. Our research reveals that the components of the Hedgehog pathway in annelids are more similar to vertebrates than to fruit flies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Wenfeng Xiong, Chunling Shen, Chaojie Li, Xiaohong Zhang, Haoyang Ge, Lingyun Tang, Yan Shen, Shunyuan Lu, Hongxin Zhang, Mi Han, Aijun Zhang, Jinjin Wang, Youbing Wu, Jian Fei, Zhugang Wang
Summary: The interaction between PRSS37 and other molecular chaperones and ADAM proteins in sperm maturation sheds light on the potential mechanisms of male infertility, providing new insights for contraception and treatment strategies.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Bidisha Das, Sumangal Roychowdhury, Priyesh Mohanty, Azamat Rizuan, Joy Chakraborty, Jeetain Mittal, Krishnananda Chattopadhyay
Summary: The misfolding and mutation of Cu/Zn superoxide dismutase (SOD1) is commonly associated with amyotrophic lateral sclerosis (ALS). This study found that the absence of Zn leads to structural disorder within two loop regions of SOD1, triggering SOD1 liquid-liquid phase separation (LLPS) and amyloid formation. The addition of exogenous Zn to metal-free SOD1 or severe ALS mutation I113T stabilizes the loops and impairs SOD1 LLPS and aggregation. Partial Zn-mediated inhibition of LLPS was observed for another severe ALS mutant, G85R, which shows perturbed Zn-binding.
Article
Genetics & Heredity
Delfien Syx, Yoshihiro Ishikawa, Jan Gebauer, Sergei P. Boudko, Brecht Guillemyn, Tim Van Damme, Sanne D'hondt, Sofie Symoens, Sheela Nampoothiri, Douglas B. Gould, Ulrich Baumann, Hans Peter Bachinger, Fransiska Malfait
Summary: Osteogenesis imperfecta is caused by a homozygous p.(R222S) substitution in the HSP47 gene, resulting in significantly reduced affinity of HSP47-R222S for type I collagen and leading to overmodification of type I collagen. Unlike other types of OI, this overmodification is not due to prolonged exposure of collagen to modifying enzymes, as the intracellular folding rate of type I collagen appears to be normal. Abnormal binding of HSP47-R222S results in upregulation and increased binding of molecular chaperones and collagen-modifying enzymes, suggesting a compensatory mechanism for aberrant binding and eventually leading to overmodification of type I collagen chains.
Article
Microbiology
Steven J. Jensen, Zachary C. Ruhe, August F. Williams, Dinh Q. Nhan, Fernando Garza-Sanchez, David A. Low, Christopher S. Hayes
Summary: The tli immunity gene disruption of Enterobacter cloacae induces hyperpermeability phenotype through unopposed activity of the Tle phospholipase effector. Disruption of the tli lipoprotein signal sequence prevents immunity protein localization to the periplasm. Periplasmic Tli acts as a canonical immunity factor to neutralize incoming effector proteins.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kevin J. Snead, Landon L. Moore, Christina R. Bourne
Summary: Type-II toxin-antitoxin systems consist of two tightly interacting proteins that must remain paired to protect the host cell from toxicity. Studies have shown that antitoxin amino acids play a crucial role in the protein-protein interaction kinetics and affinity.
Article
Multidisciplinary Sciences
Young-Yoon Lee, Haedong Kim, V. Narry Kim
Summary: RNA silencing depends on Dicer's specific and efficient processing of double-stranded RNA to produce microRNAs and small interfering RNAs. A new study found evidence for an additional sequence-dependent determinant, called the "GYM motif", which promotes processing at a specific position and enhances RNA interference. The C-terminal double-stranded RNA-binding domain of DICER recognizes the GYM motif, and alterations in this domain affect miRNA processing and cleavage sites.
Article
Biochemistry & Molecular Biology
Marcin Bielecki, Svetlana Antonyuk, Richard W. Strange, Klaudia Sieminska, John W. Smalley, Pawel Mackiewicz, Michal Smiga, Megan Cowan, Michael J. Capper, Paulina Slezak, Mariusz Olczak, Teresa Olczak
BIOCHEMICAL JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Daisuke Sasaki, Tatiana F. Watanabe, Robert R. Eady, Richard C. Garratt, Svetlana Antonyuk, S. Samar Hasnain
Summary: This study identified unique structural and functional characteristics of Cu-containing nitrite reductases, demonstrating that the 4-domain BrNiR utilizes N-terminal tethering to downregulate enzymatic activity. Compared to classical 2-domain CuNiRs, BrNiR and its variants showed significant differences in catalytic efficiency and activity levels.
Article
Biochemical Research Methods
Jiraporn Panmanee, Svetlana V. Antonyuk, S. Samar Hasnain
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Daisuke Sasaki, Tatiana F. Watanabe, Robert R. Eady, Richard C. Garratt, Svetlana Antonyuk, S. Samar Hasnain
Article
Multidisciplinary Sciences
Gareth S. A. Wright, Tatiana F. Watanabe, Kangsa Amporndanai, Steven S. Plotkin, Neil R. Cashman, Svetlana V. Antonyuk, S. Samar Hasnain
Review
Biochemistry & Molecular Biology
Gareth S. A. Wright
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Biographical-Item
Chemistry, Multidisciplinary
Henry N. Chapman, S. Samar Hasnain, Uwe Weierstall
Article
Biochemistry & Molecular Biology
Chai C. Gopalasingam, S. Samar Hasnain
Summary: Metalloproteins are a large portion of proteins that use the redox properties of transition metals. The development of advanced storage ring sources and X-ray free-electron lasers has revolutionized metalloprotein crystallography. Cryogenic electron microscopy single-particle analysis has also been widely used in the past decade, especially for membrane-bound metalloprotein systems. This article explores recent advancements in metalloprotein crystallography and cryogenic electron microscopy, focusing on the contributions of each technique and their ability to preserve the redox states of metals.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana S. Luis, Arnaud Basle, Dominic P. Byrne, Gareth S. A. Wright, James A. London, Chunsheng Jin, Niclas G. Karlsson, Gunnar C. Hansson, Patrick A. Eyers, Mirjam Czjzek, Tristan Barbeyron, Edwin A. Yates, Eric C. Martens, Alan Cartmell
Summary: This study uses structural biology to uncover how sulfatases from the human gut microbiota recognize sulfated glycans. The data reveals that carbohydrate sulfatases are highly selective for the glycan component of their substrate.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alex J. Flynn, Svetlana V. Antonyuk, Robert R. Eady, Stephen P. Muench, S. Samar Hasnain
Summary: The cryoEM structure of qNOR from Alcaligenes xylosoxidans provides insights into its crucial role in the denitrification pathway and sheds light on the mechanisms of electron, substrate, and proton transport.
NATURE COMMUNICATIONS
(2023)
Letter
Chemistry, Multidisciplinary
Richard Henderson, Samar Hasnain
Summary: Structural biology benefits from an expanding toolkit, particularly cryoEM, which provides unprecedented insight into the assembly and organization of multi-protein machineries. CryoEM has significantly advanced the determination of membrane proteins and large protein complexes structures, allowing the examination of fundamental questions regarding enzyme mechanisms. It is important for the scientific community to engage and clarify the definition of cryoEM to ensure consistency in literature.
Article
Chemistry, Medicinal
Juliana O. de Souza, Suzete M. Almeida, Guilherme E. Souza, Camila L. Zanini, Everton M. da Silva, Juliana Calit, Daniel Y. Bargieri, Kangsa Amporndanai, Svetlana Antonyuk, S. Samar Hasnain, Fabio C. Cruz, Dhelio B. Pereira, Glaucius Oliva, Arlene G. Corre, Anna C. C. Aguiar, Rafael V. C. Guido
Summary: The study reports the synthesis and antiplasmodial profiling of a series of 4(1H)-quinolone derivatives, showing inhibitory activities against sensitive and resistant strains of Plasmodium falciparum. The frontrunner compound 4 (LSPN182) demonstrated potent and selective inhibition against the cytochrome bc1 complex, as well as considerable activity against clinical field isolates of both P. falciparum and P. vivax. These findings suggest that 4(1H)-quinolone derivatives are promising candidates for antimalarial drug discovery.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Gareth S. A. Wright
Summary: The internalization of a bacteria by an archaeal cell accelerated eukaryotic evolution by utilizing a specific copper chaperone to activate CuZnSOD for combating oxidative stress. The adoption of a single protein interface with structural characteristics necessary for homodimerization and heterodimerization played a crucial role in evolution.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biology
Varunya Chantadul, Gareth S. A. Wright, Kangsa Amporndanai, Munazza Shahid, Svetlana V. Antonyuk, Gina Washbourn, Michael Rogers, Natalie Roberts, Matthew Pye, Paul M. O'Neill, S. Samar Hasnain
COMMUNICATIONS BIOLOGY
(2020)