Article
Plant Sciences
Hongwei Xu, Yingbo Li, Runhong Gao, Rugen Xu, Guimei Guo, Ruiju Lu, Nigel G. Halford, Zhiwei Chen, Chenghong Liu
Summary: The study improved the nitrogen use efficiency (NUE) of barley line DH45 through F-1 microspore embryogenesis and hydroponic field experiments, and investigated the molecular mechanisms behind its superior NUE compared to its parents using RNA-seq analysis. The results revealed enrichment of genes involved in nitrogen compound metabolism in DH45 under low nitrogen conditions.
Article
Plant Sciences
Siyu Fang, Jie Li, Wenfeng Zheng, Zhiyong Liu, Hui Feng, Yun Zhang
Summary: Isolated microspore culture is used for breeding of flowering Chinese cabbage but low embryogenesis frequency has limited its practical application. This study successfully established an efficient microspore culture protocol using different concentrations of sodium nitrophenol and 5-nitrophenol to enhance embryogenesis and plant regeneration rates. The doubled haploid rates of the genotypes reached close to 63%, and the DH lines showed high uniformity and self-incompatibility, making them suitable for hybrid breeding. This method accelerates the use of microspore culture in hybrid breeding of flowering Chinese cabbage.
Article
Biotechnology & Applied Microbiology
Weishu Chen, Yun Zhang, Shengnan Huang, Jie Ren, Hui Feng
Summary: Isolated microspore culture (IMC) is a potential alternative technique in plant breeding for producing homozygous lines. However, low embryogenesis rate, high embryo death rate, and low chromosome doubling frequency limit its implementation. This study investigated the effects of L-ascorbic acid sodium salt (VcNa) concentrations on embryogenesis and regeneration in kale using IMC, and developed an artificial chromosome doubling protocol for kale microspore-derived haploids. The results showed that 4 μM VcNa at pH 5.84 was the optimum concentration for embryogenesis, and dipping haploid plantlet roots in colchicine solution at specific concentrations and durations achieved nearly 50% chromosome doubling rate. This study provides an effective tissue culture process for promoting chromosomal ploidy of microspore-derived regenerated plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Horticulture
Jialei Ji, Henan Su, Wenxue Cao, Xiaoli Zhang, Hongrun Li, Zhiyuan Fang, Limei Yang, Yangyong Zhang, Mu Zhuang, Yong Wang, Vasiliy Taranov, Honghao Lv
Summary: In this study, QTL mapping was conducted using 109 DH lines derived from a cross between the highly embryogenic genotype 01-88 and the nonembryogenic genotype 02-12. The results showed that a QTL locus controlling cabbage embryogenesis ability was mapped to an interval of 57.4-58.4 Mb on chromosome C03. Furthermore, 12 genes with nonsynonymous mutations were identified in the candidate interval, with the DEFECTIVE KERNEL1 (DEK1) gene being identified as an important candidate gene. This study provides a foundation for cloning genes controlling cabbage microspore embryogenesis and the application of microspore culture technology in cabbage breeding on a large scale.
SCIENTIA HORTICULTURAE
(2023)
Review
Biotechnology & Applied Microbiology
Madhu Patial, Ruchi Chauhan, Harinder K. Chaudhary, Kallol K. Pramanick, Arun K. Shukla, Vishnu Kumar, Ramesh P. S. Verma
Summary: The development and improvement of biotechnological tools have greatly contributed to the progress in barley breeding program. The use of doubled haploids has significantly shortened the cultivar development time and has been widely used in various genetic studies. However, challenges such as low efficiency and genotypic specificity still need to be addressed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Multidisciplinary Sciences
Byomkesh Dash, Sudhansu Sekhar Bhuyan, Sandeep Kumar Singh, Manjusha Chandravani, Nibedita Swain, Prachitara Rout, Jawahar Lal Katara, C. Parameswaran, B. N. Devanna, Sanghamitra Samantaray
Summary: Rice is crucial for global food security. This study developed an androgenic protocol for indica rice, contributing to yield improvement.
Article
Biochemistry & Molecular Biology
Haixiao Hu, Yujie Meng, Wenxin Liu, Shaojiang Chen, Daniel. E. E. Runcie
Summary: Recent advances in maize DH technology enable the efficient development of large numbers of DH lines. Phenotyping of haploid progenitors can accelerate the selection of DH lines. Multi-trait models improve genomic prediction accuracy for DH lines, but incorporating information from haploid lines does not further improve the prediction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Lei Du, Hao Zhang, Wangsen Xin, Kejun Ma, Dengxiang Du, Changping Yu, Yongzhong Liu
Summary: By investigating two maize populations, we identified multiple quantitative trait loci related to flowering time and height traits. These consensus QTLs and other repeatedly detected loci provide valuable information for further genetic studies and variety improvements.
Article
Plant Sciences
Anna Mineykina, Ludmila Bondareva, Alexey Soldatenko, Elena Domblides
Summary: The study successfully carried out the entire cycle from inducing embryogenesis to regenerating DH plants of red cabbage, achieving a successful cultivation effect by selecting optimal parameters and factors.
Article
Agronomy
Iara Goncalves dos Santos, Anderson Luiz Verzegnazzi, Jode Edwards, Ursula K. Frei, Nicholas Boerman, Leandro Tonello Zuffo, Luiz P. M. Pires, Gerald de la Fuente, Thomas Lubberstedt
Summary: This study demonstrates that spontaneous haploid genome doubling does not affect the performance of inbred lines and that BS39 genes have the potential to add novel alleles to temperate elite germplasm.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Surbhi Grewal, Veronica Guwela, Claire Newell, Cai-yun Yang, Stephen Ashling, Duncan Scholefield, Stella Hubbart-Edwards, Amanda Burridge, Alex Stride, Ian P. King, Julie King
Summary: This study generated 86 stable inherited doubled haploid (DH) wheat-T. urartu introgression lines, which were characterized using genotyping arrays and KASP markers to identify unique introgressions in a bread wheat background. The DH lines have accelerated the breeding process and enabled the early release of homozygous wheat-T. urartu introgression lines, which can greatly advance the identification of beneficial alleles for wheat improvement.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Alejandro Ledesma, Fernando Augusto Sales Ribeiro, Alison Uberti, Jode Edwards, Sarah Hearne, Ursula Frei, Thomas Lubberstedt
Summary: The molecular characterization analysis confirmed the loss of genetic variability from C0 to C17 through the recurrent selection process in maize breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Rabouan Oubaidou, Samira Hentour, Chafika Houasli, Rachid Aboutayeb, Youness El Goumi, Ezzouhra El Maaiden, Fatima Gaboun, Ouafa Lamsaouri, Malika Fakiri
Summary: Salt stress is a major environmental factor affecting cereal productivity, and selecting salt tolerant genotypes is an effective way to overcome yield losses. This study compared phenotypic variability among doubled haploid barley lines and their parent under different salinity conditions. Results showed that traits like plant height and number of grains per spike play significant roles in determining grain yield, with number of fertile spikes per plant and number of grains per spike being indicative of salt tolerance in barley.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2021)
Article
Agronomy
Jahad Soorni, Zahra-Sadat Shobbar, Danial Kahrizi, Federica Zanetti, Kaveh Sadeghi, Sara Rostampour, Peter Gergo Kovacs, Attila Kiss, Iman Mirmazloum
Summary: This study investigated the diverse agronomic traits of 136 doubled haploid (DH) camelina lines, including seed yield, fatty acid composition, and protein content. The results showed significant diversity among the lines for these traits. The positive relationships observed suggest the potential for developing higher-yielding camelina cultivars with high seed oil content. The study also identified promising genotypes for further breeding to increase seed yield and improve food/nonfood traits.
Article
Horticulture
Peng Jia, Rui Yan, Yuan Wang, Feng-huan Gao, Yang Liu, Qing-long Dong, Hao-an Luan, Xue-mei Zhang, Han Li, Su-ping Guo, Guo-hui Qi
Summary: The KNOX gene plays crucial roles in plant development, and this study identified 19 KNOX gene members in kiwifruit. One of the genes, AcKNOX11, is selectively expressed in flower buds and shoots, and its expression can be induced by ABA and melatonin. Functional analysis revealed that AcKNOX11 delays flowering, reduces plant height, alters leaf shape, and inhibits seed germination through ABA-melatonin interaction.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Dan Hua, Wen-Shu Chen, Rong-Yu Rao, Xu-Feng Chen, Huan-Huan Chen, Ning-Wei Lai, Lin-Tong Yang, Xin Ye, Li -Song Chen
Summary: This study revealed that nitrogen deficiency can lead to increased production of reactive oxygen species (ROS) and methylglyoxal (MG), but also enhances the ability of leaves and roots to detoxify these molecules. The effects of nitrogen deficiency on ROS and MG generation and their detoxification systems were found to be different in leaves and roots, with roots being more affected.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Shenghan Yang, Chonlong Chio, Wensheng Qin, Yanxi Pei, Guangpeng Pei, Yuen Zhu, Hua Li
Summary: This study evaluates the immobilization and deactivation of pathogen-produced enzymes and toxic metabolites by biochar in tomato Fusarium wilt and finds that biochar can effectively prevent disease occurrence.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Deborah Pagliaccia, Michelle Ortiz, Michael Rodriguez, Sophia Abbott, Agustina De Francesco, Madison Amador, Valeria Lavagi, Benjamin Maki, Francesca Hopkins, Jonathan Kaplan, Samantha Ying, Georgios Vidalakis
Summary: This study investigates the effectiveness of organic soil amendments (bokashi, biochar, and their combination) in promoting soil health, nutrient availability, and plant growth. The results show that these amendments can significantly alter soil parameters and have positive effects on soil and plant health.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yu-Jeong Kwon, Min Jeong Hong, Minkyu Park, Dawon Jeon, Gah-Hyun Lim, Sungyul Chang, Dong-Hwan Kim, Jin-Baek Kim, Jundae Lee, Yeong Deuk Jo
Summary: Flowing LOCUS T (FT)-like genes play crucial roles in flowering induction in plants. Pepper FT-like genes are mostly expressed in leaves and interact with FLOWERING LOCUS D, promoting or delaying flowering. The unique evolutionary process of pepper FT-like genes provides insights for improving productivity.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Ziwen Su, Juan Yan, Binbin Zhang, Meng Sun, Zhixiang Cai, Zhijun Shen, Ruijuan Ma, Mingliang Yu
Summary: Bagging is a crucial cultivation measure that affects the quality of peach fruit by regulating exposure to light. This study investigated the effects of bagging on the content of phenolic compounds in peach fruit and identified the most sensitive compounds to bagging treatment. The study also explored the regulatory role of bagging on phenolic synthetic-related genes. The results showed that bagging affected the accumulation of different phenolics in different peach cultivars and light intensity played a significant role in the accumulation of anthocyanins and other phenolic substances in peach fruit. Additionally, the expression of structural genes and transcription factors related to phenolic synthesis was regulated by bagging and light conditions. These findings provide a foundation for understanding the regulation mechanism of light on the synthesis of phenolic compounds in peach fruit.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yanfang Zhang, Xinzhi Cui, Zhilei Du, Xiulian Li, Bangdi Liu, Meng Liu, Xiangquan Zeng, Fengjun Guo, Xinguang Fan, Shuyang Sun
Summary: In this study, melatonin treatment effectively improved the aroma quality of apricots during low temperature storage by maintaining firmness and soluble solids content, inhibiting ethylene production, and regulating enzyme activities related to aroma synthesis.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Sang-Mo Kang, Shifa Shaffique, Md. Injamum-Ul-Hoque, Sarah Owdah Alomrani, Yong-Sung Park, In-Jung Lee
Summary: Salinity is a global environmental problem that affects the physiology and morphology of plants. Melatonin has been found to improve photosynthesis, antioxidant activities, and seedling characteristics in milk thistle plants exposed to salinity stress, thereby enhancing their salt tolerance.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Nadia Borgini, Haifa Benmoussa, Mohamed Ghrab, Mehdi Ben Mimoun
Summary: This study investigates the agroclimatic requirements of Prunus species cultivars growing in warm areas using Partial Least Squares regression. The findings reveal that the chilling and heat requirements of the cultivars appear discontinuous, with overlaps or transition periods between the two phases. The warm mean temperatures occurring during the chilling period are the main determinant of the flowering of the studied cultivars.
SCIENTIA HORTICULTURAE
(2024)
Review
Horticulture
Kayise Hypercia Maseko, Thierry Regnier, Belinda Meiring, Obiro Cuthbert Wokadala, Tonna Ashim Anyasi
Summary: Bananas are a significant tropical fruit with diverse cultivars, providing essential minerals, vitamins, and phytochemicals. The Cavendish group is preferred, but identifying suitable cultivars and maturity stages is crucial for various applications.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Godfrey Ouma, Joshua Wanyama, Isa Kabenge, Joseph Jjagwe, Mukulu Diana, John Muyonga
Summary: Irrigation plays a crucial role in increasing eggplant yields amidst climate change, but the deficit irrigation level for optimal growth remains uncertain. This study examined the effect of deficit drip irrigation on eggplant performance and found that irrigating at 75% field capacity maximizes yield and water saving in sandy clay loam soil.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Andrea Marcone, Giorgio Impollonia, Michele Croci, Henri Blandinieres, Stefano Amaducci
Summary: This study used Sentinel-2 satellite images to estimate biophysical and biochemical parameters of spinach crops and found that canopy-level parameters were estimated more accurately. The estimation of the canopy-scale parameter AGB using MCARI showed the highest accuracy, while the estimation of the leaf-scale parameter LCC using NDWI showed the lowest accuracy. At the field scale, the validation results for AGB estimation using SR were the best.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Chengsheng Gong, Guangjun Guo, Baogui Pan, Changzhou Gao, Xianwei Zhu, Shubin Wang, Jinbing Liu, Weiping Diao
Summary: This study investigated the role of key cytokines and metabolic factors in the formation of plant-type traits in pepper. Through integrated analysis of RNA-seq and metabolite determination, the study identified the low content of gibberellin and high content of auxin as important factors causing plant dwarfing, and jasmonic acid as a metabolic factor affecting branch traits. A total of 131 candidate genes involved in metabolite synthesis and the formation of plant-type traits were identified.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yue Ma, Jia-Chen Hu, Yang Yu, Xin Cheng, Yan-Li Du, Qiang Zhao, Ji-Dao Du
Summary: Cadmium and drought stress significantly inhibited the growth of adzuki bean seedling plants, with the combined stress showing the highest degree of inhibition. Cd+D treatment reduced Cd accumulation in adzuki bean seedling roots. The treatment altered antioxidant enzyme activities and cell cycle phases in the roots, leading to changes in reactive oxygen species content.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Lucette Adet, Danae M. A. Rozendaal, Arthur Tapi, Pieter A. Zuidema, Philippe Vaast, Niels P. R. Anten
Summary: This study investigated the effects of water stress on cocoa trees and found that they are highly sensitive to water stress, with different genotypes exhibiting varied responses. Potassium application did not mitigate the negative effects of water stress. There was significant variation among genotypes in terms of leaf and crown traits, and positive interactions between genotype and irrigation were observed. These findings provide insights into the acclimation strategies of cocoa trees and can be useful for selecting drought-tolerant genotypes.
SCIENTIA HORTICULTURAE
(2024)