Article
Infectious Diseases
Li Wei, Yu Feng, Hongxia Wen, Hu Ya, Fu Qiao, Zhiyong Zong
Summary: This study identified a common carbapenem-resistant Klebsiella pneumoniae (CRKP) lineage in neonates that caused various infections and had been circulating in multiple hospitals for several years. The emergence of this lineage was likely due to the acquisition of a self-transmissible carbapenemase-encoding gene. The findings highlight the potential differences between CRKP lineages in neonates and adults.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Parnrada Nulsopapon, Worapong Nasomsong, Manat Pongchaidecha, Dhitiwat Changpradub, Piraporn Juntanawiwat, Wichai Santimaleeworagun
Summary: The study on antibiotic sensitivity and pharmacokinetic/pharmacodynamic data of CRKP and CoRKP suggests that increasing antibiotic treatment doses and combination therapy may be effective strategies against these bacteria.
Article
Immunology
Sanjay Singh, Ashutosh Pathak, Mohibur Rahman, Avinash Singh, Soumyabrata Nag, Chinmoy Sahu, Kashi Nath Prasad
Summary: The study identified that colistin resistance in Klebsiella pneumoniae is mainly mediated by mcr-1. The co-existence of colistin, carbapenem, and other drug-resistant genes along with efflux pumps suggests significant genomic plasticity in K. pneumoniae, potentially leading to the emergence of super-spreaders of drug resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Po-Han Huang, Yi-Hsiang Cheng, Wen-Yin Chen, Chih-Han Juan, Sheng-Hua Chou, Jin-Town Wang, Chien Chuang, Fu-Der Wang, Yi-Tsung Lin
Summary: This study investigated the risk factors and mechanisms for in vivo emergence of colistin resistance in CRKP, revealing colistin exposure as a significant factor predisposing to resistance. Alterations in the mgrB gene were identified as the predominant mechanism for emergent colistin resistance.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Biochemistry & Molecular Biology
Hao Guo, Yuye Wu, Lirong Li, Jianfeng Wang, Juan Xu, Fang He
Summary: The study investigated the global dissemination trends of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains that co-carry multiple carbapenemases. A CRKP strain, KP424, co-carrying blaNDM-1 and blaKPC-2, was identified and sequenced. Genome sequences of CRKP strains carrying multiple carbapenemase genes were retrieved from the NCBI GenBank database. The prevalence of CRKP strains co-carrying two carbapenemase genes has significantly increased over time, with strains carrying both blaNDM and blaOXA-48-like being the most common worldwide. Further research is needed to understand the mechanisms of multiple carbapenemase gene occurrence and prevent their global dissemination.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Immunology
Miaomiao Xie, Kaichao Chen, Ning Dong, Qi Xu, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Summary: Study findings showed that colistin-resistant K. pneumoniae strains had increased fitness and biofilm formation potential in vitro, as well as higher survival rates in the presence of normal human serum. Interestingly, these strains exhibited reduced virulence in a mouse infection model but enhanced virulence in a Galleria mellonella infection model. Infection with colistin-resistant strains also resulted in lower expression levels of inflammatory cytokines and significantly decreased bacterial loads.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Po-Han Huang, Wen-Yin Chen, Sheng-Hua Chou, Fu-Der Wang, Yi-Tsung Lin
Summary: Treatment of carbapenem-resistant Klebsiella pneumoniae (CRKP) infections is challenging due to limited antibiotic options. The development of colistin resistance during treatment poses a major challenge. This study found that the addition of tigecycline to colistin treatment reduced the risk of acquiring colistin resistance, providing hope for the treatment of CRKP infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Jozef Ficik, Michal Andrezal, Hana Drahovska, Miroslav Bohmer, Tomas Szemes, Adriana Liptakova, Livia Slobodnikova
Summary: The COVID-19 era has significantly increased the incidence of carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospitals. It is vital to monitor the global and local incidence of CRKP and its antimicrobial resistance. The study highlighted the importance of rational antibiotic policy and strict anti-epidemic precautions.
Article
Chemistry, Medicinal
Tsung-Ying Yang, Hao-Yun Kao, Po-Liang Lu, Pei-Yu Chen, Shu-Chi Wang, Liang-Chun Wang, Ya-Ju Hsieh, Sung-Pin Tseng
Summary: AS101, repurposed as an antimicrobial agent, showed significant antibacterial effects against colistin- and carbapenem-resistant Klebsiella pneumoniae strains, indicating its potential for treating drug-resistant bacteria.
Article
Infectious Diseases
Ilker Inanc Balkan, Mustafa Alkan, Gokhan Aygun, Mert Kuskucu, Handan Ankarali, Alper Karagoz, Sumeyye Sen, Hatice Yasar Arsu, Mehtap Bicer, Sibel Yildiz Kaya, Ridvan Karaali, Bilgul Mete, Nese Saltoglu, Fehmi Tabak
Summary: Mortality due to K. pneumoniae bacteremia is increasing, especially in regions with high resistance to carbapenem and colistin. Risk factors for colistin resistance and its impact on mortality were studied in patients with CRKp bacteremia. Colistin resistance was associated with hospital stay duration and prior colistin use, and significantly increased 28-day mortality along with age. Combined antimicrobial therapy was found to be more effective than monotherapy in reducing mortality in CRKp bacteremia.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Immunology
Xi Jin, Qiong Chen, Fang Shen, Yan Jiang, Xueqing Wu, Xiaoting Hua, Ying Fu, Yunsong Yu
Summary: This study reported a case of hv-CRKP-associated infection and revealed the in-host evolution of resistance to tigecycline and polymyxin during clinical therapy. The results suggest that the hypervirulent ST11-KL64 clone poses a potential threat to anti-infection treatment with rapid and diverse evolution of resistance.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Infectious Diseases
Wajiha Imtiaz, Zainab Syed, Zara Rafaque, Simon Colin Andrews, Javid Iqbal Dasti
Summary: The study identified high levels of antibiotic resistance in clinical K. pneumoniae isolates from tertiary care hospitals in Islamabad and Rawalpindi, Pakistan, with significant prevalence of MDR-CRKP and hypermucoviscous traits. The presence of resistance to fosfomycin, the mcr-1 and mcr-2 genes in colistin-resistant isolates, and the detection of rmpA gene in CRKP clinical isolates highlight emerging resistance patterns and virulence factors in these strains.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Yali Gong, Yifei Lu, Dongdong Xue, Yu Wei, Qimeng Li, Gang Li, Shuguang Lu, Jing Wang, Yunying Wang, Yizhi Peng, Yan Zhao
Summary: This study describes the emergence of a carbapenem-resistant Klebsiella pneumoniae (CRKP) strain, KP2e, in a Chinese hospital. The strain, recovered from a fatal septic shock case, carried the NDM-6 gene and exhibited resistance to almost all beta-lactams except for monobactam. It belonged to sequence type 4024 and had a genome composed of one chromosome and three plasmids. Coexistence of bla(NDM-6) on two self-transmissible plasmids, belonging to types IncFIB and IncN, was observed. The study highlights the importance of monitoring and detecting bla(NDM)-harbouring plasmids.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Arnon Chukamnerd, Rattanaruji Pomwised, Kongpop Jeenkeawpiam, Chanida Sakunrang, Sarunyou Chusri, Komwit Surachat
Summary: This study elucidates the genetic features of carbapenem-resistant Klebsiella pneumoniae (CRKP) clinical isolates from a university hospital in Thailand, indicating the presence of various antimicrobial resistance and virulence genes, as well as genomic diversity. The findings highlight the importance of whole-genome sequencing (WGS) in the rapid and accurate genomic analysis of clinical isolates of CRKP.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Yujiao Wang, Bo Zhu, Min Liu, Xiutao Dong, Jianping Ma, Xiaofeng Li, Fang Cheng, Jianzhuang Guo, Sumei Lu, Furong Wan, Yingying Hao, Wanshan Ma, Mingju Hao, Liang Chen
Summary: The study reported three Klebsiella species strains carrying TmexCD2-ToprJ2, indicating the spread of this novel plasmid-mediated tigecycline resistance mechanism to different Klebsiella species. The co-occurrence of bla(NDM-1) and TmexCD2-ToprJ2 in the same plasmid is of particular public health concern.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jinane Tabcheh, Julia Vergalli, Anne Davin-Regli, Noha Ghanem, Jean-Marie Pages, Charbel Al-Bayssari, Jean Michel Brunel
Summary: Antibiotic resistance is increasing morbidity and mortality for non-curable infectious diseases. Novel strategies, such as effective antibiotic combinations and antibiotics with non-antibiotic activity-enhancing compounds, have emerged to combat multidrug-resistant microorganisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Mohamad Yasmin, Laura J. Rojas, Steven H. Marshall, Andrea M. Hujer, Anna Cmolik, Emma Marshall, Helen W. Boucher, Alejandro J. Vila, Maxime Soldevila, Seydina M. Diene, Jean-Marc Rolain, Robert A. Bonomo
Summary: This study characterized the drug resistance mechanisms and evaluated the efficacy of contemporary antibiotics against Elizabethkingia anophelis, an emerging pathogen causing life-threatening infections in immunocompromised patients. Whole-genome sequencing identified drug-resistant genes and determined the susceptibility of the pathogen to different antibiotics. The study provides important guidance for selecting appropriate antimicrobial therapy.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Rima El-Basst, Sanaa Saliba, Lama Saleh, Nicolas Saoud, Eid Azar, Pierre Zalloua, Amanda Chamieh
Summary: This retrospective study analyzed the resistance patterns and trends of 1827 Pseudomonas isolates in a tertiary medical center in Lebanon from January 2010 to December 2018. The incidence of Pseudomonas aeruginosa infection in sputum samples significantly decreased, while there was no trend in bloodstream infection. The study also found a decrease in resistance to certain antibiotics and a reduction in the consumption of antipseudomonal antibiotics. Antimicrobial stewardship had a positive impact on nosocomial epidemiology.
Article
Infectious Diseases
Khouloud Slimene, Allaaeddin El Salabi, Olfa Dziri, Najla Mathlouthi, Seydina M. Diene, Elhussan Ahmed Mohamed, Jadalla M. A. Amhalhal, Mohammed O. Aboalgasem, Jomaa F. Alrjael, Jean-Marc Rolain, Chedly Chouchani
Summary: Antimicrobial resistance, especially to carbapenems, poses a major threat to public health. A study conducted in Ibn Sina Hospital in Libya identified low susceptibility to carbapenems among Gram-negative bacteria. Molecular investigations revealed the presence of New Delhi metallo-beta-lactamases-1 and Verona integron-encoded metallo-beta-lactamase enzymes in Pseudomonas spp. Additionally, a new gene and resistance gene were discovered in Acinetobacter baumannii.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Microbiology
Sarrah Landolsi, Rachid Selmi, Linda Hadjadj, Asma Ben Haj Yahia, Lilia Messadi, Jean-Marc Rolain
Summary: A study conducted in Tunisia found that rodents can potentially transmit multidrug resistant bacteria, posing a public health problem. The researchers isolated Enterobacteriaceae strains from the intestines of rats captured from various locations and found that rodents may play a role in the spread of antimicrobial resistant E. coli.
VETERINARY MICROBIOLOGY
(2023)
Editorial Material
Microbiology
Ziad Daoud, Jean-Marc Rolain
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Khouloud Slimene, Asrra A. Ali, Elhussan A. Mohamed, Allaaeddin El Salabi, Faraj S. Suliman, Agela A. Elbadri, Fadi F. El-fertas, Ahmed El-awjly, Salah A. Shokri, Jean-Marc Rolain, Chedly Chouchani
Summary: The emergence of carbapenem-resistant bacteria in SARS-CoV-2 patients in eastern Libya highlights the need for increased surveillance and rational use of antibiotics, particularly carbapenems, during the pandemic. Multidrug-resistant strains, including K. pneumoniae, A. baumannii, and colistin-resistant E. coli, were identified, emphasizing the importance of maintaining the efficacy of these antibiotics and minimizing their overuse.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Sabah El-Sawalhi, Oceane Revol, Amanda Chamieh, Alexandre Lacoste, Alexandre Annessi, Bernard La Scola, Jean-Marc Rolain, Isabelle Pagnier
Summary: This study examines the circulation of bacterial strains and their antibiotic resistance genes in the aquatic environment in Marseille, France. The importance of monitoring the frequency of these circulating bacteria in water treatments is emphasized.
MICROBIOLOGY SPECTRUM
(2023)
Letter
Infectious Diseases
May Khoder, Marwan Osman, Issmat I. Kassem, Rayane Rafei, Ahmad Shahin, Seydina M. Diene, Jean-Marc Rolain, Monzer Hamze
NEW MICROBES AND NEW INFECTIONS
(2023)
Correction
Public, Environmental & Occupational Health
Thi Loi Dao, Van Thuan Hoang, Amal Magmoun, Tran Duc Anh Ly, Sophie Alexandra Baron, Linda Hadjadj, Naomie Canard, Tassadit Drali, Frederique Gouriet, Didier Raoult, Philippe Parola, Pierre Marty, Jean-Marc Rolain, Philippe Gautret
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Microbiology
Mohamad Maatouk, Vicky Merhej, Pierre Pontarotti, Ahmad Ibrahim, Jean-Marc Rolain, Fadi Bittar
Summary: The Candidate Phyla Radiation (CPR) harbors a large number of genes encoding enzymes with potential antibiotic resistance activity, particularly metallo-beta-lactamase-like (MBL-like) enzymes. These CPR MBL-like sequences are longer and more diverse than bacterial MBLs, and they have a high content of functional domains. Most CPR MBL-like sequences have not been identified with known MBLs, indicating the presence of many potential, yet undiscovered, functions in CPR.
Article
Microbiology
Habibou Sarr, Aissatou Ahmet Niang, Amadou Diop, Oleg Mediannikov, Hanane Zerrouki, Seydina M. Diene, Seynabou Lo, Mouhamadou Lamine Dia, Ahmad Iyane Sow, Florence Fenollar, Jean-Marc Rolain, Linda Hadjadj, Carla Calia, Lawrence S. Young
Summary: This study investigated the antibiotic resistance of enterobacteria in Senegal and identified the emergence of carbapenemase-producing Enterobacterales (CPE) infections. The results showed that carbapenemase genes and class A β-lactamase genes were widely present in enterobacteria. This phenomenon is concerning, and strict surveillance is necessary to prevent further spread.
Article
Infectious Diseases
Irene Sanchez-Leon, Teresa Garcia-Martinez, Seydina M. Diene, Elena Perez-Nadales, Luis Martinez-Martinez, Jean-Marc Rolain
Summary: This study aimed to investigate heteroresistance in nine clinical isolates of Klebsiella pneumoniae producing OXA-48 and describe genomic changes in vitro. It was found that the heteroresistance in K. pneumoniae isolates was mainly attributed to insertions in the mgrB gene and point mutations in colistin resistance proteins. These findings contribute to the understanding of colistin resistance mechanisms in OXA-48-producing K. pneumoniae mutants.
Article
Infectious Diseases
Ayan Ali Ragueh, Mohamed Houmed Aboubaker, Sitani Idriss Mohamed, Jean-Marc Rolain, Seydina M. Diene
Summary: This study investigated the resistance rate to carbapenems among multidrug-resistant Gram-negative bacteria (MDR-GNB) in Djibouti and characterized their resistance mechanisms. The results showed that out of 256 isolates, 20 were resistant to carbapenems. Carbapenemase genes such as bla(OXA-48) and bla(NDM-5) were identified in the isolates.
