Article
Engineering, Environmental
Yuxin Zhuo, Wenjuan Xu, Yuan Chen, Feng Long
Summary: In this study, a mobile microfluidic lab-on-fiber device called SMILE was developed for rapid and accurate detection of AFB1 in food samples. SMILE showed high sensitivity, specificity, and reproducibility, making it a promising tool for point-of-need testing of AFB1 and other trace contaminants.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Chemistry, Applied
Qi Chen, Meng Meng, Wang Li, Ying Xiong, Yong Fang, Qinlu Lin
Summary: This review highlights the recent advances in immunosensors, aptasensors, and other emerging receptor-based sensors for the detection of AFM(1) in milk and dairy products. It discusses their unique advantages, drawbacks, and future opportunities and challenges.
Article
Environmental Sciences
Yanping Liu, Yuyang Chen, Wenjuan Xu, Dan Song, Xiangzhi Han, Feng Long
Summary: This paper presents an improved portable and reusable evanescent wave optofluidic immunosensor (OIP-v2) for rapid and sensitive on-site determination of deoxynivalenol (DON), a frequently detected mycotoxin produced by Fusarium species. The OIP-v2 showed a high limit of detection and linear dynamic detection range under optimal conditions. It also demonstrated good correlation with the enzyme-linked immunosorbent assay (ELISA) when detecting maize-positive samples.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biophysics
Jinchuan Liu, Yunpeng Xing, Xiaohong Zhou, George Y. Chen, Hanchang Shi
Summary: A novel U-shaped fiber-optic evanescent-wave fluorescent immunosensor was designed for real-time detection of microcystin-LR using light-sheet excitation of skew rays. The sensor showed enhanced sensitivity with the use of light-sheet skew rays, leading to successful establishment of a sensitive real-time MC-LR detection method. The system demonstrated high recovery rates for MC-LR detection in environmental water samples, showing potential for on-site environmental monitoring and clinical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Physical
Xudong Zhao, Ni Yao, Xianghua Zhang, Lei Zhang, Guangming Tao, Zijian Li, Quan Liu, Xiujian Zhao, Yinsheng Xu
Summary: This study proposes a new approach of tapered-fiber geometry optimization to improve the evanescent efficiency of mid-infrared chalcogenide fiber sensors. The influence of fiber geometry on sensing performance is discussed, and the analysis reveals that a smaller waist radius and longer taper length lead to higher evanescent efficiency. Additionally, the investigation of waist deformation shows that increasing the waist boundary radius can decrease the sensing performance.
Article
Agriculture, Dairy & Animal Science
Rafael Mora-Medina, Antonio Jesus Lora-Benitez, Ana Maria Molina-Lopez, Nahum Ayala-Soldado, Rosario Moyano-Salvago
Summary: In recent years, there has been a growing concern about milk pollution by aflatoxin M1 due to the increasing prevalence of aflatoxins in animal feeding and dairy product consumption. A study was conducted to determine the transfer of aflatoxin B1 into milk and its effects on production and serological parameters in goats. Goats were exposed to different doses of aflatoxin B1 for 31 days and the concentration of aflatoxin M1 in milk was found to significantly increase with the amount of aflatoxin B1 ingested. However, the ingested amount did not affect the aflatoxin M1 carryover, indicating a certain resistance of goats to the effects of aflatoxin B1.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Marzieh Hajmohammadi, Reza Valizadeh, Abassali Naserian, Mohammad E. Nourozi, Carlos A. F. Oliveira
Summary: The study found that using raw mineral clays with appropriate particle sizes can effectively reduce the content of AFM(1) in dairy cow milk, with a reduction effect equivalent to commercial adsorbents. This method can provide a more economical alternative to reduce the transfer rate of AFM(1) to dairy cow milk.
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Gege Yu, Jiamin Wang, Yao Zhang, Haofen Wu, Yueqi Wang, Yan Cui, Yuefan Yang, Xiaoqian Tang, Qi Zhang, Jianlong Wang, Jing Sun, Ran Chen, Yanru Wang, Peiwu Li
Summary: Nanobodies are small and stable, and can be used as antigen substitutes in immunoassays. This study investigated the recognition mechanism between nanobody and antiaflatoxin monoclonal antibody, and developed a highly sensitive fluorescent immunosensor for aflatoxin quantification based on this mechanism.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Agriculture, Dairy & Animal Science
Li Min, Johanna Fink-Gremmels, Dagang Li, Xiong Tong, Jing Tang, Xuemei Nan, Zhongtang Yu, Weidong Chen, Gang Wang
Summary: Milk is considered a perfect natural food for humans and animals, but toxins in the milk from dairy cows may pose risks to human health. Further research is needed to understand the metabolism of toxins in dairy cow feed and their effects on milk.
Article
Food Science & Technology
I Salazar, I Lopez, P. Glorio-Paulet, C. Gomez
Summary: Limited research on aflatoxin contamination in Peru focused on detecting AFB(1) and AFM(1) levels, with a study conducted in a typical intensive dairy farm in Northern Peru revealing seasonal variations. The study found that December had higher levels of AFM(1) contamination in milk and AFB(1) in feedstuffs. Commonly used feedstuffs like maize, soybean meal, and whole soybean showed varying levels of AFB(1) contamination.
WORLD MYCOTOXIN JOURNAL
(2021)
Article
Chemistry, Applied
Guangming Li, Chen Liu, Xingcai Zhang, Pengjie Luo, Guimiao Lin, Wenxiao Jiang
Summary: A facile hydrothermal method was used to synthesize highly photoluminescent N-doped carbon dots with quantum yields reaching 97.1%. An label-free immunosensor based on the inner filter effect of carbon dots was developed for ultrasensitive detection of aflatoxin M1 residues in milk, with a detection limit of 0.0186 ng/mL. The immunosensor showed good linear relationship, high recoveries, and low relative standard deviations, making it a reliable on-site screening method for aflatoxin M1 residue analysis.
Article
Mycology
D. Costamagna, M. Gaggiotti, M. L. Signorini
Summary: A quantitative risk assessment was conducted on exposure to aflatoxin M1 (AFM(1)) in milk and dairy products in Argentina. The study found that while certain populations may face higher risks of exposure, overall the impact on the health of infants, toddlers, and children is not significant.
MYCOTOXIN RESEARCH
(2021)
Article
Biochemical Research Methods
Xue Zhang, Xiaoning Liao, Yongfa Wu, Wanming Xiong, Juan Du, Zhui Tu, Wuying Yang, Dan Wang
Summary: A label-free electrochemical immunosensor for small molecular AFB(1) detection was constructed using functionalized black phosphorene and anti-AFB(1) nanobody, showing a linear range from 1.0 pM to 5.0 nM and an ultralow detection limit of 0.27 pM under optimized conditions. The fabricated immunosensor exhibited satisfactory stability, specificity, and reproducibility, providing a reliable reference for label-free sensing of small molecules in food samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Ningning Xu, Yingping Xiao, Qinggang Xie, Yang Li, Junan Ye, Daxi Ren
Summary: In this study, the occurrence of aflatoxin B1 (AFB1) in total mixed rations (TMR) and aflatoxin M1 (AFM1) in raw and commercial milk in northern China during winter season was investigated. Results showed that all samples from northern China were below the legal limit for AFB1 and AFM1 in raw and commercial milk, with only about 30% of samples exceeding the EU legal limit. However, the northwest area of China had higher AFM1 levels and variance in milk samples, indicating a need for more attention to aflatoxin contamination in this region.
Article
Agriculture, Dairy & Animal Science
A. Kihal, M. Rodriguez-Prado, S. Calsamiglia
Summary: The objective of this network meta-analysis was to determine the efficacy of different mycotoxin binders (MTB) to reduce aflatoxin M1 (AFM1) in milk. A literature search identified 28 papers with 131 data points, and the binders used in the studies were hydrated sodium calcium aluminosilicate (HS CAS), yeast cell wall (YCW), bentonite, and mixes of several MTB (MX). The results showed that all MTB reduced the AFM1 transfer into milk, with bentonite having the highest capacity and YCW the lowest.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)