Article
Chemistry, Multidisciplinary
Yu Chen, Weipeng Lu, Yanchuan Guo, Yuntao Xie, Yi Zhu, Yeping Song
Summary: Combining ROS-scavenging and antibacterial activities, multifunctional gelatin/chitosan composite microspheres effectively alleviate oxidative stress and bacterial invasion in chronic wounds. The gelatin/chitosan scaffold with good biocompatibility promotes cell proliferation.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Microbiology
Razieh Kebriaei, Katherine L. Lev, Rahi M. Shah, Kyle C. Stamper, Dana J. Holger, Taylor Morrisette, Ashlan J. Kunz Coyne, Susan M. Lehman, Michael J. Rybak
Summary: Bacterial biofilms are difficult to eradicate and can complicate infections. Phage+antibiotic combinations (PAC) may be more effective on biofilms than single agents. Our study demonstrated the effectiveness of phage Sb-1 on MRSA biofilms and suggested PAC regimens as adjuncts to antibiotics.
MICROBIOLOGY SPECTRUM
(2022)
Review
Engineering, Biomedical
Francois Berthiaume, Henry C. Hsia
Summary: This article describes new experimental approaches, including biophysical techniques and biochemical methods, to improve the regenerative response of skin. Various treatment approaches exist to help heal chronic skin wounds, but the healed tissue often lacks mechanical properties and appendages. Therefore, harnessing the potential of stem cells to regenerate the skin appears to be a promising option. These approaches primarily focus on the local wound environment and should be combined with other interventions addressing regional and systemic diseases and social determinants of health.
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Camila Mendes Figueiredo, Marilia Silva Malvezzi Karwowski, Romeu Cassiano Pucci da Silva Ramos, Nicoly Subtil de Oliveira, Lorena Caroline Pena, Everdan Carneiro, Renata Ernlund Freitas de Macedo, Edvaldo Antonio Ribeiro Rosa
Summary: Microbial biofilms are challenging to control due to limited accessibility of antimicrobial drugs. This review discusses the advantages, recommendations, and limitations of using bacteriophages as 'biofilm predators', and the feasibility of using phages to control biofilms in different environments.
Article
Cell Biology
Michelle S. Bach, Christiaan R. de Vries, Arya Khosravi, Johanna M. Sweere, Medeea C. Popescu, Qingquan Chen, Sally Demirdjian, Aviv Hargil, Jonas D. Van Belleghem, Gernot Kaber, Maryam Hajfathalian, Elizabeth B. Burgener, Dan Liu, Quynh-Lam Tran, Tejas Dharmaraj, Maria Birukova, Vivekananda Sunkari, Swathi Balaji, Nandini Ghosh, Shomita S. Mathew-Steiner, Mohamed S. El Masry, Sundeep G. Keswani, Niaz Banaei, Laurence Nedelec, Chandan K. Sen, Venita Chandra, Patrick R. Secor, Gina A. Suh, Paul L. Bollyky
Summary: This study reveals that a bacteriophage called Pf, produced by Pseudomonas aeruginosa, delays healing of chronic wounds by inhibiting the migration of keratinocytes, which is essential for wound closure. The presence of Pf-positive strains of Pseudomonas aeruginosa in chronic wounds is associated with a higher likelihood of wound progression. These findings suggest that Pf may serve as a biomarker and potential therapeutic target for chronic wounds.
CELL REPORTS MEDICINE
(2022)
Article
Medicine, General & Internal
Vincent Falanga, Roslyn Rivkah Isseroff, Athena M. Soulika, Marco Romanelli, David Margolis, Suzanne Kapp, Mark Granick, Keith Harding
Summary: Chronic wounds are wounds that do not heal within an expected time frame due to prolonged dysregulated inflammation. Advances in understanding the cellular and molecular aspects of basic science and innovative treatment modalities have improved the management of chronic wounds. However, gaps still exist in scientific knowledge, highlighting the need for further research to enhance understanding of chronic wounds.
NATURE REVIEWS DISEASE PRIMERS
(2022)
Review
Cell Biology
Jordan Holl, Cezary Kowalewski, Zbigniew Zimek, Piotr Fiedor, Artur Kaminski, Tomasz Oldak, Marcin Moniuszko, Andrzej Eljaszewicz
Summary: With the increasing prevalence of type 2 diabetes mellitus, the management of chronic diabetic wounds and ulcers has become a significant therapeutic challenge. Recent advancements in understanding wound healing mechanisms have led to the development of novel therapies, with a particular focus on biological dressings.
Article
Dermatology
Sita M. Damaraju, Benjamin R. Mintz, J. Genevieve Park, Ankur Gandhi, Sunil Saini, Joseph A. Molnar
Summary: This study evaluated the feasibility of a one-stage procedure using skin substitutes combined with autologous skin cell suspension in a porcine full-thickness wound healing model. Addition of autologous skin cell suspension to DS1 or DS2 resulted in a significant increase in wound re-epithelialization, indicating that this approach may be a viable one-stage treatment in clinical practice.
INTERNATIONAL WOUND JOURNAL
(2022)
Review
Pharmacology & Pharmacy
Karan Razdan, Jorge Garcia-Lara, V. R. Sinha, Kamalinder K. Singh
Summary: This review focuses on the mechanism of biofilm formation and the role of biofilms in chronic wounds, as well as various formulation strategies and delivery systems used to eradicate or disperse biofilms. The clinical research on the treatment of biofilm-infected chronic wounds is also discussed.
DRUG DISCOVERY TODAY
(2022)
Article
Biotechnology & Applied Microbiology
Aparna Nair, Renuka Vyawahare, Krishna Khairnar
Summary: The novel bacteriophage En5822 isolated from the environment shows high lytic activity, specificity, and biofilm reduction capability against Enterobacter cloacae. Its genomic and physical attributes make it a potential alternative antimicrobial for treating drug-resistant infections.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Nicole M. Vecin, Robert S. Kirsner
Summary: Chronic wounds like diabetic foot ulcers and venous leg ulcers are a significant burden on the healthcare system and can have comparable mortality rates to cancer. They negatively affect patients' quality of life due to pain, odor, decreased mobility, and social isolation. Skin substitutes are recommended for non-healing wounds, and the choice of substitute should be evidence-based, taking into account the etiology of the wound. There are currently over 75 available skin substitutes, and this number is increasing.
FRONTIERS IN MEDICINE
(2023)
Review
Microbiology
Cheng Chang, Xinbo Yu, Wennan Guo, Chaoyi Guo, Xiaokui Guo, Qingtian Li, Yongzhang Zhu
Summary: Biofilms are complex microbial microcolonies that are resistant to antibiotics and can adhere to various surfaces. Using bacteriophages and their components for biofilm removal shows promise, but there are still challenges to overcome.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Dermatology
Paulo J. Alves, Ruben T. Barreto, Brigitte M. Barrois, Luc G. Gryson, Sylvie Meaume, Stan J. Monstrey
Summary: Biofilms play a major role in delaying chronic wounds from healing and pose a significant treatment challenge due to their resistance towards antimicrobial agents. However, there is a window of opportunity post-debridement when biofilms are more susceptible to topical antiseptics. Among commonly used antiseptics, PVP-I stands out for its broad spectrum of antimicrobial activity, potent antibiofilm efficacy, lack of acquired resistance, low cytotoxicity, good tolerability, and wound healing promotion.
INTERNATIONAL WOUND JOURNAL
(2021)
Article
Engineering, Biomedical
Shin-Da Wu, Niann-Tzyy Dai, Chao-Yaug Liao, Lan-Ya Kang, Yu-Wen Tseng, Shan-hui Hsu
Summary: A bioprintable hydrogel has been developed for the treatment of chronic and irregular wounds. The hydrogel can be printed in both planar and curvilinear shapes, and has been shown to promote complete repair and regeneration in both types of wounds.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Microbiology
Michelle Marie Esposito, Sara Turku
Summary: Biofilms are large aggregates of various species of bacteria or other microorganisms tightly attached to surfaces through an intricate extracellular matrix. These complex microbial communities present significant challenges in the food processing industry due to the potential for contamination and the resistance of biofilm growth to traditional antimicrobials. Natural methods, such as bacteriocins, bacteriophages, fungi, phytochemicals, and plant extracts, have been explored as potential means to target foodborne biofilms.
Review
Chemistry, Multidisciplinary
Catarina Milho, Jani Silva, Rafaela Guimaraes, Isabel C. F. R. Ferreira, Lillian Barros, Maria Jose Alves
Summary: Oral microbial biofilms are directly linked to oral diseases, and the search for natural products with bioactive potential from plants is crucial in preventing the growth of oral bacteria. Some medicinal plants show promise in inhibiting the formation of biofilms.
APPLIED SCIENCES-BASEL
(2021)
Article
Infectious Diseases
Ana Mafalda Pinto, Alberta Faustino, Lorenzo M. Pastrana, Manuel Banobre-Lopez, Sanna Sillankorva
Summary: Pseudomonas aeruginosa is a major pathogen responsible for nosocomial and chronic infections, with increasing antibiotic resistance posing a challenge for treatment. Bacteriophage therapy has shown promise as an alternative, with this study highlighting the importance of careful selection and combination of phages in formulations to maximize efficacy and prevent emergence of resistant bacteria.
Review
Infectious Diseases
Rafaela Guimaraes, Catarina Milho, Angela Liberal, Jani Silva, Carmelia Fonseca, Ana Barbosa, Isabel C. F. R. Ferreira, Maria Jose Alves, Lillian Barros
Summary: The historical use of natural products for promoting health has been increasingly highlighted, with plant extracts being explored as potential sources for new pharmaceutical formulations. The resistance of Candida fungi to conventional antimicrobials has led to the search for new molecules in plant extracts as alternative agents for infection prevention and treatment.
Article
Nanoscience & Nanotechnology
Marta Ribeiro, Meriem Boudoukhani, Efres Belmonte-Reche, Nuria Genicio, Sanna Sillankorva, Juan Gallo, Carlos Rodriguez-Abreu, Nadji Moulai-Mostefa, Manuel Banobre-Lopez
Summary: This study introduces a biocompatible hydrogel with magnetic nanoparticles, allowing controlled drug release and non-invasive monitoring, which is crucial for precise treatment of internal diseases.
ACS APPLIED NANO MATERIALS
(2021)
Article
Virology
C. Milho, S. Sillankorva
Summary: In this study, the influence of deleting a gene with unknown function on the replication parameters of Salmonella phage PVP-SE2 was assessed using Bacteriophage Recombineering of Electroporated DNA. The results showed that deletion of the specific gene affected the replication process of the phage and led to a smaller progeny release compared to the wild-type phage.
Article
Microbiology
Maria Daniela Silva, Antonio Lima, Nuno Marcal, Luis Dias, Miguel Gama, Sanna Sillankorva
Summary: Understanding the bacterial causes of otitis media is crucial for effective treatment. This study analyzed samples from 49 children with otitis media in Portugal, finding a correlation between bacteria in the middle ear fluid and nasopharynx. The most commonly identified species were Moraxella catarrhalis and Streptococcus pneumoniae, but Haemophilus influenzae was more prevalent in the middle ear fluid. The study also revealed the importance of polymicrobial infections in otitis media.
Article
Pharmacology & Pharmacy
Maria Daniela Silva, Kaelin Ray, Miguel Gama, Aaron K. Remenschneider, Sanna Sillankorva
Summary: The article discusses the increasing bacterial resistance to antibiotics and the need for alternative antimicrobial compounds and local drug delivery methods. The study evaluates the permeation ability of liposomes loaded with the pneumococcal endolysin MSlys and the free MSlys on human tympanic membranes. The results show that MSlys loaded in PEGylated liposomes have increased permeation across the membranes and can reduce the pneumococcal cell load. However, the antipneumococcal activity decreases after 4 hours of permeation. The study provides insights into non-invasive delivery of endolysins for local treatment of otitis media.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Virology
Sanna Sillankorva, Liliana Pires, Lorenzo M. Pastrana, Manuel Banobre-Lopez
Summary: The prevalence of drug-resistant bacteria in chronic skin wounds and biofilm-related infections is a major concern. This study demonstrates the potential of using dissolving microneedles loaded with bacteriophages to effectively combat biofilm-related skin infections.
Article
Genetics & Heredity
Jessica C. Sousa, Sanna Sillankorva, Alberta Faustino, Carla M. Carvalho
Summary: This work describes a newly isolated Klebsiella pneumoniae phage with unique features, providing new insights into the diversity and evolutionary pathways of Klebsiella phages.
Review
Microbiology
Ana Oliveira, Carla Dias, Ricardo Oliveira, Carina Almeida, Pablo Fucinos, Sanna Sillankorva, Hugo Oliveira
Summary: Escherichia coli is a notorious pathogen that can adapt, colonize, and proliferate in different environments. Its presence in the food industry causes food poisoning and imposes economic burden on the food, agriculture, and health sectors. Bacteriophages have emerged as a promising strategy to control E. coli pathogens without harming humans and animals. This review comprehensively analyzes the research on E. coli phages for veterinary, food, and human use, discussing their potential as a One Health approach and exploring advanced technologies for phage-based control strategies.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Jonata M. Ueda, Catarina Milho, Sandrina A. Heleno, Anton Soria-Lopez, Maria Carpena, Maria J. Alves, Tania Pires, Miguel A. Prieto, Jesus Simal-Gandara, Ricardo C. Calhelha, Isabel C. F. R. Ferreira, Lillian Barros
Summary: Infectious diseases and antibiotic resistance are significant concerns for human health. Continuous medical research is needed to address the emergence of new infectious diseases and the increasing resistance of bacterial strains. MRSA is an example of a bacterial strain that has developed resistance against commonly used antibiotics, making the treatment of infections caused by MRSA limited and challenging. Researchers are exploring natural resources, such as plants, to discover bioactive molecules that can inhibit or kill MRSA, due to their high antimicrobial properties.
CURRENT PHARMACEUTICAL DESIGN
(2023)
Article
Biochemistry & Molecular Biology
Marcia Keller Alves, Wellington Vieira de Souza, Junia Capua de Lima Novello, Sanna Sillankorva, Cilene Labre, Rubem Luis Sommer, Joao Antonio Pegas Henriques, Mariana Roesch Ely
Summary: A novel bacteriophage infecting Pseudomonas cichorii, a plant phytobacterium, was isolated from leafy vegetables in Brazil. Its genome size is 40,117 bp and shows high sequence identity with other Pseudomonas phages.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Virology
Maria J. Costa, Lorenzo M. Pastrana, Jose A. Teixeira, Sanna M. Sillankorva, Miguel A. Cerqueira
Summary: Currently, one-third of all food produced worldwide is wasted or lost, largely due to bacterial contamination which is also a major cause of foodborne diseases. New solutions are necessary to address these issues and enhance food safety. Bacteriophages (phages) are a potential solution for bacterial contamination as they are harmless to humans and can prevent or reduce food contamination by foodborne pathogens. However, the use of phages in their free form may result in decreased effectiveness. Therefore, research is being conducted to develop new delivery systems that can ensure prolonged activity and controlled release of phages in food systems.
Article
Pharmacology & Pharmacy
K. B. Vinayakumar, Maria Daniela Silva, Artur Martins, Stephen Mundy, Pedro Gonzalez-Losada, Sanna Sillankorva
Summary: Additive manufacturing advancements have significant contributions to various fields, and it is gaining attention in the medical field for its customizable, low-cost, and fast drug delivery and diagnostic tool development. This study reports the fabrication of a microneedle platform using a stereolithography 3D printer, with varying printing angles and aspect ratios. The optimal printing angle of 30 degrees resulted in needle tip and base diameters of approximately 50 and approximately 330 μm, with heights of approximately 550/850/1180 μm. Levofloxacin-loaded polyvinyl alcohol (PVA) microneedles showed gradual release of the drug against Klebsiella pneumoniae biofilms. 3D printing is an attractive solution for producing molds to fabricate biopolymeric microneedles for topical drug delivery.
ADVANCED THERAPEUTICS
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)