Review
Chemistry, Applied
Kelsey Wilson, Anthony Atala
Summary: This article focuses on the application of three-dimensional printing in the medical field and discusses the strengths and weaknesses of different types of printers. It also explores the challenges and future opportunities in tissue engineering using 3D printing.
ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Yuxi Guo, Xuefeng Chen, Pin Gong, Guoliang Li, Wenbo Yao, Wenjuan Yang
Summary: The gut is a vital digestive and immune organ where microorganisms coexist with the host. The gut microbiota interacts with the host's immune system, affecting the function of other organs. The gut-on-chip technology has been developed to emulate the structure and function of the human gut, providing insights into gut-related interactions and pathophysiological studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Pedro Lavrador, Vitor M. Gaspar, Joao F. Mano
Summary: Engineered living materials are a new generation of human-made biotherapeutics with attractive features, providing encodable bioactivities, extended lifetimes, and environmental adaptability. Novel cell bioengineering tools are driving the assembly of multiscale living materials by utilizing living cells as cooperative building blocks. These materials have the potential to improve precision and personalized medicine strategies in the future.
Review
Chemistry, Analytical
Lijuan Ma, Nan Li, Jing Wang, Chaofu Ma, Xiaoyan Hu, Mingshuang Li, Zhisheng Wu
Summary: Strong research and development barriers and steep patent cliffs have driven the need for higher efficiency and cost-effectiveness in pharmaceutical analysis. Microfluidic platforms have emerged as promising tools in pharmaceutical analysis due to their high precision, accuracy, throughput, efficiency, reagent consumption, detection speed, and automation. This review introduces the applications of microfluidic chips in drug analysis, including rapid offline analysis, real-time and online analysis, model organism cultivation, and organ-on-a-chip/organoids-on-chips analysis, aiming to provide novel insights and effective guidance for future research in pharmaceutical analysis using microfluidic platforms.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Oncology
Christina Bao Xian Huang, Ting-Yuan Tu
Summary: The vasculature plays a critical role in cancer progression and metastasis, and organ-on-a-chip technology has emerged as a robust tool for creating cancer models in vitro. This review discusses the latest developments in 3D microfluidic vascularized tumor models and their applications in studying cellular interactions, metastasis mechanisms, and therapeutic screening. The review also examines the benefits, drawbacks, and future prospects of these models.
FRONTIERS IN ONCOLOGY
(2023)
Review
Pharmacology & Pharmacy
Isabella Francis, Jesus Shrestha, Keshav Raj Paudel, Philip M. Hansbro, Majid Ebrahimi Warkiani, Suvash C. Saha
Summary: The transition from 2D to 3D models and the recent advances in lung-on-a-chip platforms, their implementation, and the challenges faced are discussed, highlighting the potential applications in respiratory disease research and drug discovery.
DRUG DISCOVERY TODAY
(2022)
Review
Biotechnology & Applied Microbiology
Chrysanthi-Maria Moysidou, Chiara Barberio, Roisin Meabh Owens
Summary: Research in cell biology heavily relies on in vitro assays and models, with the recent advancement in 3D cell biology and engineering providing more physiologically relevant culture platforms. These platforms, such as scaffold-based systems and organs-on-chips, show great potential in bridging the gap between conventional animal studies and human physiology by allowing for more accurate modeling of specific processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Behavioral Sciences
Fotios Athanasopoulos, Orionas-Vasilis Saprikis, Myrto Margeli, Christoph Klein, Nikolaos Smyrnis
Summary: Research on oculomotor function in schizophrenia suggests it as a potential candidate for biomarkers, but current data are insufficient to unequivocally support its use in clinical practice.
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Markus Wild, Jintawee Kicuntod, Lisa Seyler, Christina Wangen, Luca D. Bertzbach, Andele M. Conradie, Benedikt B. Kaufer, Sabrina Wagner, Detlef Michel, Jan Eickhoff, Svetlana B. Tsogoeva, Tobias Bauerle, Friedrich Hahn, Manfred Marschall
Summary: Through experiments on various PKIs, this study demonstrated the potential efficacy of these kinase inhibitors not only against HCMV but also potentially against other herpesviruses. Drug combination experiments revealed a strong synergistic effect between LDC4297 and maribavir, opening up new possibilities for future clinical treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Meilin Sun, Kai Han, Rui Hu, Dan Liu, Wenzhu Fu, Wenming Liu
Summary: The article discusses the importance of micro/nanoscale porous membrane materials in biomedical applications and provides an overview of various membrane fabrication techniques. It also explores the practical applications of these membranes in fields such as cell and biomolecule separation, biomimicking, and biosensing.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biophysics
Hojjatollah Nazari, Jesus Shrestha, Vahid Yaghoubi Naei, Sajad Razavi Bazaz, Milad Sabbagh, Jean Paul Thiery, Majid Ebrahimi Warkiani
Summary: Biological barriers are multicellular structures that regulate the transport of various substances. Transendothelial/epithelial electrical resistance (TEER) is a label-free method used to predict barrier properties. Integrating TEER measurement with organ-on-a-chip technology provides valuable insight into barrier behavior and enhances TEER measurement accuracy. This review discusses the applications, advantages, and future perspectives of integrating organ-on-a-chip technology with TEER measurement methods.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Mark J. Lynch, Oliviero L. Gobbo
Summary: Nanotheranostics represent a novel drug delivery system for improving systemic and brain-targeted delivery of diagnostic imaging agents and pharmacological moieties. The challenge lies in effectively evaluating the blood-brain barrier permeation and biocompatibility of nanomaterials to treat neurological disorders.
Review
Chemistry, Analytical
Xingxing Liu, Qiuping Su, Xiaoyu Zhang, Wenjian Yang, Junhua Ning, Kangle Jia, Jinlan Xin, Huanling Li, Longfei Yu, Yuheng Liao, Diming Zhang
Summary: Cancer, as one of the leading causes of death worldwide, presents challenges in understanding its nature and developing effective therapeutics due to its unclear molecular mechanism and complex tumor microenvironment. The organ-on-chip (OoC) platform, integrating 3D cell culture, tissue engineering, and microfluidics, emerges as a new method to simulate the complex tumor microenvironment and has significant implications for personalized treatment and drug development.
Review
Biotechnology & Applied Microbiology
Hyeon Ryoo, Hannah Kimmel, Evi Rondo, Gregory H. Underhill
Summary: Cellular phenotypes and functional responses are regulated by signals present in their microenvironment. High throughput culture platforms can better recapitulate and analyze these complex signals. Various techniques such as microplates, microarrays, and microfluidic cell culture systems have been developed for high throughput cell culture. Current applications include stem cell sourcing, drug discovery, and personalized medicine.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Biomedical
Leyla Amirifar, Amir Shamloo, Rohollah Nasiri, Natan Roberto de Barros, Ze Zhong Wang, Bige Deniz Unluturk, Alberto Libanori, Oleksandr Ievglevskyi, Sibel Emir Diltemiz, Samuel Sances, Ilangko Balasingham, Stephanie K. Seidlits, Nureddin Ashammakhi
Summary: Recent advances in biomaterials, microfabrication, microfluidics, and cell biology have led to the development of organ-on-a-chip devices that can replicate key functions of various organs. These platforms promise to provide novel insights into physiological events, including disease mechanisms, and evaluate the impact of external interventions such as drug administration. Therefore, the neuroscience field is expected to benefit greatly from these innovative tools.
Article
Multidisciplinary Sciences
S. R. A. Kratz, C. Eilenberger, P. Schuller, B. Bachmann, S. Spitz, P. Ertl, M. Rothbauer
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Analytical
Patrick Schuller, Mario Rothbauer, Sebastian R. A. Kratz, Gregor Hoell, Phillipp Taus, Markus Schinnerl, Jakob Genser, Neus Bastus, Oscar H. Moriones, Victor Puntes, Berthold Huppertz, Monika Siwetz, Heinz Wanzenboeck, Peter Ertl
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Multidisciplinary
Hadi Hashemzadeh, Abdollah Allahverdi, Mosslim Sedghi, Zahra Vaezi, Tahereh Tohidi Moghadam, Mario Rothbauer, Michael Bernhard Fischer, Peter Ertl, Hossein Naderi-Manesh
Article
Spectroscopy
Mario Rothbauer, Christoph Eilenberger, Sarah Spitz, Barbara Bachmann, Jasmin Pajenda, Andreas Schwaighofer, Gregor Hoell, Palle Steen Helmke, Yvonne Kohl, Bernhard Lendl, Peter Ertl
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2020)
Article
Cell Biology
Katharina M. Pichler, Anita Fischer, Juergen Alphonsus, Catharina Chiari, Sebastian Schmidt, Michael Kenn, Wolfgang Schreiner, Daniela Weinmann, Mario Rothbauer, Reinhard Windhager, Hans-Joachim Gabius, Stefan Toegel
Summary: Galectin-4 (Gal-4) is a galactose-binding protein that is upregulated in osteoarthritis, leading to the upregulation of pro-degradative/-inflammatory proteins in chondrocytes. This study suggests that targeting galectin activity may be a potential therapeutic approach in OA progression.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Cell Biology
Hadi Hashemzadeh, Ali Hamad Abd Kelkawi, Abdollah Allahverdi, Mario Rothbauer, Peter Ertl, Hossein Naderi-Manesh
Summary: The inadequacy of animal models has led to the development of improved in vitro models, such as 3D multicellular spheroids, for drug efficacy assessments. While these models provide physiological relevance and meet high-throughput and low-cost requirements for drug discovery, their application in nanotoxicity testing is still in its early stages due to limited penetration and uptake rates. Comparative studies between 2D monolayer cultures and 3D spheroids revealed distinct cellular responses to gold nanowires (AuNWs) treatment, emphasizing the need for more physiologically relevant tissue models for investigating nanomaterial-biology interactions.
Article
Pharmacology & Pharmacy
Christoph Eilenberger, Mario Rothbauer, Konstanze Brandauer, Sarah Spitz, Eva-Kathrin Ehmoser, Seta Kuepcue, Peter Ertl
Summary: Stem cell technology and embryonic stem cell models are crucial for studying neurogenesis and early brain development. However, the lack of standardization and comparability in the establishment of embryoid bodies (EBs) hinders the broader application of stem cell technology. This study focuses on optimizing differentiation protocols and the size of EBs to enhance the reliability of neurodevelopmental in vitro models.
Article
Biochemistry & Molecular Biology
Helene Stuetz, Eva I. Reihs, Winfried Neuhaus, Maren Pflueger, Harald Hundsberger, Peter Ertl, Christian Resch, Gerald Bauer, Guenter Povoden, Mario Rothbauer
Summary: Airborne engineered nanomaterials are by-products of industry and are considered to be important environmental toxins that can pose health risks to humans and animals. The main routes of exposure to airborne nanoparticles are through nasal and/or oral inhalation, which allow the particles to enter the bloodstream and spread throughout the body. Mucosal barriers in the nose, mouth, and lungs play a key role in preventing the translocation of nanoparticles, but there is limited understanding of their tolerance to nanoparticle exposures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Mario Rothbauer, Ruth A. Byrne, Silvia Schobesberger, Isabel Olmos Calvo, Anita Fischer, Eva Reihs, Sarah Spitz, Barbara Bachmann, Florian Sevelda, Johannes Holinka, Wolfgang Holnthoner, Heinz Redl, Stefan Toegel, Reinhard Windhager, Hans P. Kiener, Peter Ertl
Summary: Research established a tissue coculture model mimicking rheumatoid arthritis, simulating the reciprocal cross talk between synovial and chondral organoids. Results showed that when chondral organoids were co-cultivated with synovial organoids, they induced a higher degree of cartilage physiology and architecture, with differential cytokine response.
Article
Chemistry, Analytical
Ali Khodayari Bavil, Drago Sticker, Mario Rothbauer, Peter Ertl, Jungkyu Kim
Summary: An impedimetric biosensor platform was developed to enhance the sensitivity of immunoassays using microparticles as labels by measuring electrical impedance changes. Through testing various microparticles, it was found that magnetic microparticles provided high signal enhancement and stability on the sensor array. By incorporating magnetic microparticles, over ten times improvement of LOD was achieved in sandwich immunoassays.
Article
Chemistry, Analytical
Cristian Zanetti, Sarah Spitz, Emanuel Berger, Silvia Bolognin, Lisa M. Smits, Philipp Crepaz, Mario Rothbauer, Julie M. Rosser, Martina Marchetti-Deschmann, Jens C. Schwamborn, Peter Ertl
Summary: This study developed a novel electrochemical sensing approach to detect dopamine in human midbrain organoids, allowing for monitoring differences in dopamine release between healthy and Parkinson's disease-specific midbrain organoids. The practical feasibility of the sensing approach for drug screening applications and personalized disease modeling was demonstrated.
Article
Biochemical Research Methods
Michaela Purtscher, Mario Rothbauer, Sebastian Rudi Adam Kratz, Andrew Bailey, Peter Lieberzeit, Peter Ertl
Summary: Detection, quantification and monitoring of virus-host cell interactions play a crucial role in evaluating the safety of pharmaceutical products. Regulatory agencies recommend viral clearance studies to assess the efficiency of production processes in removing viruses. A microfluidic single step assay can significantly reduce assay time to 3 to 4 days, and continuous fluid flow can enhance virus-cell interactions.
Article
Biochemical Research Methods
Mario Rothbauer, Gregor Hoell, Christoph Eilenberger, Sebastian R. A. Kratz, Bilal Farooq, Patrick Schuller, Isabel Olmos Calvo, Ruth A. Byrne, Brigitte Meyer, Birgit Niederreiter, Seta Kuepcue, Florian Sevelda, Johannes Holinka, Oliver Hayden, Sandro F. Tedde, Hans P. Kiener, Peter Ertl
Review
Chemistry, Analytical
Sebastian Rudi Adam Kratz, Gregor Hoell, Patrick Schuller, Peter Ertl, Mario Rothbauer
Review
Biochemical Research Methods
Pei-Ru Chen, Peng-Fei Xia
Summary: Carbon dioxide plays a crucial role in mitigating global climate change and supporting life on Earth. Synthetic biology and metabolic engineering offer a promising approach to enhance CO2 fixation by combining the advantages of autotrophic and heterotrophic organisms. This article reviews the current progress in constructing synthetic CO2 fixation pathways, discusses the underlying design principles and challenges, and provides insights into the future of carbon recycling through engineered synthetic carbon fixation pathways.
CURRENT OPINION IN BIOTECHNOLOGY
(2024)
